Now showing 1 - 10 of 18
  • Some of the metrics are blocked by your 
    Item type:Publication,
    The family 36 carbohydrate-binding module of Paenibacillus xylaniclasticus TW1 xylanase: Characterization and recognition in epidermal tissue of sweet potato roots
    Paenibacillus xylaniclasticus TW1 was able to aerobically produce a multienzyme complex. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the crude enzyme preparation revealed at least 12 proteins that were bound to insoluble cellulose. Only the band representing cellulose-bound protein 12 (CBP12) from SDS-PAGE was identified as xylanase family 11 with a carbohydrate-binding module family 36 (CBM36) using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS). In this report, CBM36 of P. xylaniclasticus TW1 (PxTW1CBM36) was cloned, expressed, purified and studied for binding characteristic. The results found that PxTW1CBM36 displayed broad binding ability to polysaccharides which high affinity for xylan and insoluble cellulose. Interestingly, this is the first report indicated that CBM36 had an affinity for insoluble cellulose. Although the amino acid residues involved in PxTW1CBM36 binding were conserved, the binding capacity of PxTW1CBM36 do not perturb by the addition of ethylenediaminetetraacetic acid (EDTA). It is possible that PxTW1CBM36 had different binding mechanisms with other CBM36. In addition, the binding characteristic of CBM36 on polysaccharides embedded within plant cell walls was also elucidated. It displayed the strong recognition for ligands located in epidermal tissue of sweet potato roots. Therefore, this study might provide a new tool for targeting enzymes to surface of plant.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Comparative evaluation of leaf and seed methanolic extracts obtained from Sophora tomentosa Linn. for phytochemical, phenolic content, antioxidant and antibacterial activities
    (2023-01-01)
    Laowklang. Poeaim, P. S.
    ;
    Sophora tomentosa Linn. (Fabaceae), also known as a yellow necklace pod, is a traditional medicinal plant widely used to treat dysentery and diarrhea. This study evaluated the biological activities of methanolic leaf and seed extracts of S. tomentosa. The total phenolic content of methanolic leave (STL) seed (STS) and dechlorophyll of leave extract (DSTL) showed 92.02 ±1.78, 38.48 ± 0.61, and 35.35 ± 2.45 mg GAE/g extract), respectively. For antioxidant activity, DPPH, ABTS and FRAP assay was found in leaves (STL) more than in dechlorophyll of leave extract (DSTL) and seed (STS). The methanolic extracts were assessed for antibacterial activity against eight pathogenic bacterial species. The seed extract showed the most potent antibacterial activity against Kocuria rhizophila with 12.85±3.85 mm inhibition zones. For phytochemical evaluation, both leaf and seed extracts exhibited alkaloids and coumarin. The results showed that the leaf extract (STL) had higher antioxidant activity and total phenolic content than seeds and dechlorophyll of leave extract (DSTL). On the other hand, the seed extract presented the most potent antibacterial activity. The current study suggested that both leave and seed extracts of S. tomentosa gave the potential source of natural bioactive compounds, which will be further evaluated in other biological activities.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Application of organic soybean tempeh in purple sweet potato raisin cookie
    (2022-05-01) ;
    Organic soybean tempeh or tempeh kedelai is made by fermentation with Rhizopus oligosporus was used to produce cookies, as a plant-based protein snack. Four samples with various recipes - purple sweet potato cookie, purple sweet potato raisin cookie, tempeh purple sweet potato cookie and tempeh purple sweet potato raisin cookie – were prepared to determine the physicochemical and sensory features. Chemical analysis found that the tempeh purple sweet potato raisin cookie has the highest moisture (18%) and total proteins (29%). However, the purple sweet potato cookies showed the highest ashes content (2%) and tempeh purple sweet potato cookie showed the highest total lipids (29%). The tempeh purple sweet potato raisin cookie was hardest (61 N). The tempeh purple sweet potato raisin cookie had the highest scores for food acceptability of all qualities in appearance, texture, aroma, colour, taste and general acceptability using a 9-point hedonic scale ranging from slightly liked to very much liked. Therefore, the tempeh purple sweet potato raisin cookie can be accepted as a new snack, that includes a good level of protein and thus a healthy food alternative. Furthermore, if all steps of the production are carefully controlled by USDA or other certifying bodies, thus this product may be labelled as organic product.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Diversity of Sordariales Fungi: Identification of Seven New Species of Naviculisporaceae Through Morphological Analyses and Genome Sequencing
    (2025-12-01) ;
    Gautier, Valérie
    ;
    Lalanne, Christophe
    ;
    Bonometti, Lucas
    ;
    Cros-Arteil, Sandrine
    Thanks to next-generation sequencing (NGS) technologies, the diversity of fungi can now be investigated through the analysis of their genome sequences. Naviculisporaceae is a family within the Sordariales, whose diversity is not well-known, with only one genome sequence published for this family. Here, we report on the isolation and cultivation of 20 new strains of Naviculisporaceae. Their genome sequences, as well as those of the five commercially available strains, were determined, thus providing complete genome sequences for 25 new Naviculisporaceae strains. Species delimitation was conducted using a combination of (1) ITS + LSU phylogenetic analysis of the new isolates along with other known species of the family, (2) comparisons between DNA barcode sequences of the new strains with those of the known species, and (3) average genome-wide nucleotide identity calculation. We built a phylogenomic tree and studied the organization of the mating-type locus. In vitro fruiting was obtained for 16 strains, enabling the definition of seven new species, namely Pseudorhypophila gallica, Pseudorhypophila guyanensis Rhypophila alpibus, Rhypophila brasiliensis, Rhypophila camarguensis, Rhypophila reunionensis and Rhypophila thailandica, as well as two new combinations, namely Pseudorhypophila latipes and Pseudorhypophila oryzae. Eight strains for which in vitro fruiting was not obtained may belong to additional new species. These results expand the known diversity of the Naviculisporaceae and greatly enlarge the genomic data available for the family.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    The Podospora anserina (Rabenh.) Niessl Species Complex in Metropolitan and Overseas France with Description of a New Species, Podospora reunionensis Silar, sp. nov.
    (2025-12-16)
    Silar, Philippe
    ;
    Gautier, Valérie
    ;
    Lalanne, Christophe
    ;
    ;
    Arthur, Marvyn
    Podospora anserina (Rabenh.) Niessl is a model fungus that was shown to belong to a complex of seven species with morphologically indistinguishable sexual fruiting bodies. Through the morphological analyses and ITS (Intergenic Transcribed Spacer) sequences of 86 newly-isolated strains, as well as the genome sequences of five strains, we show that members of the P. anserina species complex present different distribution ranges in metropolitan and overseas France. Podospora anserina is present all-over metropolitan France, including Corsica, while P. comata Milovtz. is restricted to the Northern part and P. pauciseta (Ces.) Traverso to the Southern part of continental France. The Guadeloupe hosts P. pseudoanserina C.Boucher, T.S.Nguyen & P.Silar and La Réunion island a species new to science, described here as Podospora reunionensis Silar, sp. nov. This species is closely related to P. comata, but exhibit clear morphological and biological differences.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Evaluation of genetic diversity by molecular markers in Indian gooseberry (Phyllanthus emblica)
    (2025-01-01) ;
    Sangsoy, V.
    ;
    The genetic diversity and relationships among 16 cultivars of Indian gooseberry (Phyllanthus emblica) using Sequence-Related Amplified Polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) markers were investigated. The cultivars were collected from the Phrae Horticultural Research Center, Thailand. For SRAP analysis, 30 primer combinations were initially tested, comprising five forward and six reverse primers. Seven primer combinations consistently generated well-defined bands and were selected for genetic profiling. These SRAP primers amplified 172 fragments, with 145 (84.30%) polymorphic. For RAPD analysis, 50 primers were evaluated, and eight primers consistently produced clear bands of genetic profiling. These RAPD primers amplified 215 fragments, with 188 (87.44%) polymorphic. The genetic relationships among the cultivars were assessed using a dendrogram constructed with NTSYS-pc (version 2.1X) based on the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The genetic similarity coefficients ranged from 0.66 to 0.90 for SRAP and from 0.58 to 0.89 for RAPD markers, indicating a high level of genetic diversity among the cultivars.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Taxonomy and phylogeny of hyaline-spored coelomycetes
    (2020-01-01)
    Li, Wen Jing
    ;
    McKenzie, Eric H.C.
    ;
    Liu, Jian Kui (Jack)
    ;
    Bhat, D. Jayarama
    ;
    Dai, Dong Qin
    Coelomycete is a general term used for asexual fungi which produce conidia in fruiting bodies: pycnidial, acervular, cupulate, pycnothyria or stromatic conidiomata. The group contains numerous plant pathogenic, saprobic and endophytic species associated with a wide range of hosts. Traditionally, morphological characters and host associations have been used as criteria to identify and classify coelomycetes, and this has resulted in a poor understanding of their generic and species boundaries. DNA based taxonomic studies have provided a better outlook of the phylogenetic and evolutionary trends in coelomycetes. However, the present outcomes represent only a preliminary step towards the understanding of coelomycetes. Many genera have not been revisited since they were first described. The present study revises the classification of the hyaline-spored coelomycetes and provides a modern taxonomic framework based on both morphology and phylogeny. In total, 248 genera were investigated, of which less than 100 are known to have sequence data. Multi-locus sequence data analyses of 28S nrDNA, 18S nrDNA, ITS, RNA polymerase II second largest subunit (rpb2), and part of the translation elongation factor 1-alpha gene (tef1) and β-tubulin (tub2) gene regions were analysed. As a result, three new genera and 23 new species are introduced. In addition, three new links between sexual and asexual genera are provided. There are 138 genera that lack sequence data, and these are treated as Ascomycota, genera incertae sedis. Line drawings and descriptions are provided based on the examination of types and fresh collections and on the literature.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10
    (2023-01-01)
    Luong, Thi Thu Huong
    ;
    ;
    Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Development of Sustainable Biomaterials Composites from Waste Materials (Spent Mushroom Compost, Garlic Shell, and Water Hyacinth) with Edible Mushroom Mycelium-Based
    Purpose: To evaluate performance and add value of bio-based degradable waste materials of spent mushroom compost cooperate with garlic shell, and water hyacinth with mushroom mycelium-based methods. Method: The Ganoderma lucidum, Lentinus polychrous, L. squarrosulus, and Pleurotus sajor-caju were used to study bio-based composites from various formula of waste materials (spent mushroom compost (Agrocybe cylindracea or P. ostreatus), garlic shell, and water hyacinth). Then we selected the best species and the formula of biomaterial composites to prepare pot prototypes. Finally, the biomaterial composite pots were measured physical and mechanical properties. Results: L. squarrosulus exhibited the fastest mycelial growth and highly effective to composed materials. Then, selected further pot prototypes with variation formulation and physical and mechanical properties were presented; density ranged from 0.18−0.22 g cm<sup>-3</sup> and water absorption reaching between 172−250% in 24 hr., the absorption increases more gradually, reaching 302%−368% after 168 hr. Variations between formulation, the composites AC-F1 and PO-F1 exhibited superior water absorption. Whereas composites AC-F2 and PO-F2 demonstrated the lowest water absorption. For the compressive strength significant differences between samples prepared with spent mushroom compost from A. cylindracea and P. ostreatus. The spent mushroom compost from P. ostreatus is higher compressive strength, ranging from 385.4−451.5 kPa. In contrast the spent mushroom compost derived from A. cylindracea ranged between 174.9−197.3 kPa. Conclusion: The mycelium-based biodegradable composite produced demonstrates excellent performance and suitability for various applications. As such, they contribute to efficient upcycling and support zero-waste practices.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Antibacterial and lignocellulose-degrading enzyme activities of coprophilous fungi obtained from cow dung in Thailand
    (2024-01-01) ;
    Bhat, Darbhe Jayarama
    ;
    Twenty-seven coprophilous fungi, isolated from field-fed cow dung in an organic farm in Thailand, were identified using morphology and ITS barcode. A total of five genera viz. Aspergillus, Hamigera, Paecilomyces, Penicillium, and Talaromyces were identified with varying numbers and growth rates. These fungi were evaluated for their antibacterial properties against Gram-positive (Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and St. epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. Pe. javanicum NTD-SP2- 01 and Talaromyces sp. NTD-SP5-48 exhibited activity against all bacteria when tested with agar plug diffusion method. Talaromyces sp. NTD-SP5-48 was particularly effective against Gram-negative bacteria. As. terreus NTD-NG1-05 displayed the highest activity against five bacterial strains, except Ps. aeruginosa. Notably, As. terreus NTD-NG1-05 and Talaromyces sp. NTD-SP5-48 demonstrated extended antibacterial activity in the agar disk diffusion method, with fermented broth (FB) showing superior inhibitory effects compared to mycelial extract (MY). Both isolates demonstrated significant antibacterial activity against B. Subtilis. Furthermore, all isolates exhibited significant antibacterial activity against B. subtilis, with a diffusion of 0.125 mg/disk. Only Talaromyces sp. NTD-SP5-48 (FB) displayed the highest inhibition activity against Ps. aeruginosa, with a diffusion of 1 mg/disk (100 mg/mL). In terms of enzyme activity, all isolates exhibited cellulase activity, with Talaromyces sp. showing the highest cellulase activity, followed by As. terreus. Laccase activity was only observed in the unidentified isolate NTD-SP5-34, while none of the isolates showed pectinase activity.