Tangthirasunun, Narumon

Preferred name

Alternative Name

Tangthirasunun, N.

Main Affiliation

School of Science

narumon.ta@kmitl.ac.th

Now showing 1 - 10 of 18

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Antibacterial and lignocellulose-degrading enzyme activities of coprophilous fungi obtained from cow dung in Thailand
(2024-01-01)
Tangthirasunun, Narumon
;
Bhat, Darbhe Jayarama
;
Poeaim, Supattra
Twenty-seven coprophilous fungi, isolated from field-fed cow dung in an organic farm in Thailand, were identified using morphology and ITS barcode. A total of five genera viz. Aspergillus, Hamigera, Paecilomyces, Penicillium, and Talaromyces were identified with varying numbers and growth rates. These fungi were evaluated for their antibacterial properties against Gram-positive (Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and St. epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. Pe. javanicum NTD-SP2- 01 and Talaromyces sp. NTD-SP5-48 exhibited activity against all bacteria when tested with agar plug diffusion method. Talaromyces sp. NTD-SP5-48 was particularly effective against Gram-negative bacteria. As. terreus NTD-NG1-05 displayed the highest activity against five bacterial strains, except Ps. aeruginosa. Notably, As. terreus NTD-NG1-05 and Talaromyces sp. NTD-SP5-48 demonstrated extended antibacterial activity in the agar disk diffusion method, with fermented broth (FB) showing superior inhibitory effects compared to mycelial extract (MY). Both isolates demonstrated significant antibacterial activity against B. Subtilis. Furthermore, all isolates exhibited significant antibacterial activity against B. subtilis, with a diffusion of 0.125 mg/disk. Only Talaromyces sp. NTD-SP5-48 (FB) displayed the highest inhibition activity against Ps. aeruginosa, with a diffusion of 1 mg/disk (100 mg/mL). In terms of enzyme activity, all isolates exhibited cellulase activity, with Talaromyces sp. showing the highest cellulase activity, followed by As. terreus. Laccase activity was only observed in the unidentified isolate NTD-SP5-34, while none of the isolates showed pectinase activity.
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Assessing genetic diversity of Yellow Star tree using SRAP markers and efficacy of their endophytic fungi in biological control
(2023-05-01)
Poeaim, S.
;
Tangthirasunun, N.
;
Chareonsap, P. P.
;
Poeaim, A.
The result showed that 52 fragments (64.22%) were polymorphic bands from seven combinations of Sequence-Related Amplified Polymorphism (SRAP) markers. The genetic relationship was analyzed using the UPGMA method by NTSYpc software version 2.11X showed a similarity coefficient ranging from 0.59 to 0.98. Endophytic fungi were isolated from fresh leaves of Yellow Star (Schoutenia glomerata King subsp. peregrina (Craib) Roekm. & Hartono and identified by morphology and DNA barcoding. The fungi were identified in Alternaria alternata, Colletotrichum siamense, Diaporthe arengae, Phyllosticta capitalensis, and Xylaria cubensis. The dual culture test evaluated the efficacy of endophytic fungi isolated from Yellow Star tree against Colletotrichum gloeosporioides and Phomopsis asparagi causing disease in asparagus. The results revealed that X. cubensis had the potential to inhibit the spore germination of the C. gloeosporioides ASGC06 and P. asparagi ASGP04, which inhibited spore germination of 99.97 and 100 percent, respectively. However, this fungus should be further studied for biological control and bioactive secondary metabolites.
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Characterization of the Podospora anserina (Rabenh.) Niessl peroxidase gene family
(2024-02-15)
Ferrari, Roselyne
;
Gautier, Valérie
;
Tangthirasunun, Narumon
;
Chan Ho Tong, Laetitia
;
Silar, Philippe
Major role of peroxidases in plant biomass degradation is well-established in the white rot basidiomycetes. On the contrary, peroxidases are not used for this purpose by brown rot basidiomycetes, which use instead a non-enzymatic mechanism. In the case of the ascomycetes, not much is known although these fungi have peroxidase genes. Here, we identify and characterize the peroxidase genes of Podospora anserina (Rabenh.) Niessl, an ascomycete used to study development and lignocellulose degradation. We show that this fungus has one class II peroxidase, one hybrid B peroxidase, one haloperoxidase, four functional aromatic peroxygenases, one glutathione peroxidase, one cytochrome C peroxidase and one alkyl peroxidase, but lacks a dye peroxidase. We show that potentially secreted peroxidases (i.e., the class II, hybrid B, haloperoxidase and aromatic peroxygenase peroxidases) present a patchy phylogenetic distribution compatible with an accessory role in finely adapting the different fungal species to their ecological niche, rather than being involved in fundamental roles in fungal biology. Accordingly, targeted gene deletions of the different P. anserina peroxidase genes identified only one phenotype, seemingly an alteration of the timing of ascospore maturation at intermediate concentration of vanillic acid. However, direct measure of peroxidase activity did not show drastic loss of activity in the tested mutants, suggesting compensation between the enzymes. Hence, in P. anserina peroxidases appears to have a minor role in biomass degradation, unlike what has been described in white rot fungi, and thus in this regard appears to be similar to the brown rot fungi.
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Effective of Neosatorya to control Phomopsis asparagi causing stem blight of asparagus
(2018-12-01)
Mangkalad, T.
;
Soytong, K.
;
Tangthirasunun, N.
;
Poeaim, S.
The biocontrol of stem blight of asparagus Phomosis asparagi was proved by Neosartorya and Talaromyces. The symptoms are appearance as oval-shaped lesions with light brown centres and slightly darker margins. Ten isolates of pathogen were isolated by tissue transplanting technique and confirmed by morphology and molecular identification based on internal transcribed spacer (ITS)-nrDNA sequence as P. asparagi. Fungal mycelia showed undulate margins and colonies white to gray and reached a diameter of 9 cm petri dish after 10 days. Alpha conidia were oblong or spindle-shaped, with a size of 2‒3.5 × 6.5‒8.8 µm. The fungal pathogenicity test showed the asparagus was infected by P. asparagi within 2 days on moisture chamber at room temperature. Neosartorya and Talaromyces were proved antifungal acrtivety against P. asparagi causing of stem blight of asparagus in dual culture. It revealed that N. hiratsuka EU06 was the highest inhibition P. asparagi causing the stem blight of asparagus at 59.50%.
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Russula orientalovirescens sp. nov., a common Southeast Asian edible fungus is different from the European look-alike R. virescens
(2025-08-01)
Wisitrassameewong, Komsit
;
Adamčík, Slavomír
;
Adamčíková, Katarína
;
Tang, Song Ming
;
Tangthirasunun, Narumon
Green-cracking Russulas are edible fungi that are widely consumed and traded in Southeast Asia. Asian collections of this morphotype were frequently identified as R. virescens in local literature. Multilocus phylogenetic analyses of ITS nrDNA, rpb2 and tef1 regions presented in this study strongly supported that the majority of green cracking Russula collections from Southeast Asia represent a species different from European R. virescens and these collections are described here as R. orientalovirescens sp. nova. Analysis of ITS barcoding region confirmed that published sequence data from China, Laos and Myanmar reported this species as R. virescens. In addition, this analysis showed that the species is widely distributed in Southeast Asia from Malayan Peninsula to Japan, preferring areas with dry season, and is associated with coniferous and deciduous trees as well as heterotrophic plants. Morphological analyses and detailed comparison with recent collections of R. virescens showed that R. orientalovirescens differs from the latter by larger spores and shorter and more abundant pileocystidia. Green-cracking Russula species with distinctly areolate pileus formed a monophyletic lineage where our new species is grouped with Asian R. viridirubrolimbata, European R. virescens and North American R. parvovirescens. Few publicly available ITS sequences from Southeast Asia clustered with either European or North American species suggesting that the phylogenetic lineage of green-cracking Russulas urgently require further attention.
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The Podospora anserina (Rabenh.) Niessl Species Complex in Metropolitan and Overseas France with Description of a New Species, Podospora reunionensis Silar, sp. nov.
(2025-12-16)
Silar, Philippe
;
Gautier, Valérie
;
Lalanne, Christophe
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Tangthirasunun, Narumon
;
Arthur, Marvyn
Podospora anserina (Rabenh.) Niessl is a model fungus that was shown to belong to a complex of seven species with morphologically indistinguishable sexual fruiting bodies. Through the morphological analyses and ITS (Intergenic Transcribed Spacer) sequences of 86 newly-isolated strains, as well as the genome sequences of five strains, we show that members of the P. anserina species complex present different distribution ranges in metropolitan and overseas France. Podospora anserina is present all-over metropolitan France, including Corsica, while P. comata Milovtz. is restricted to the Northern part and P. pauciseta (Ces.) Traverso to the Southern part of continental France. The Guadeloupe hosts P. pseudoanserina C.Boucher, T.S.Nguyen & P.Silar and La Réunion island a species new to science, described here as Podospora reunionensis Silar, sp. nov. This species is closely related to P. comata, but exhibit clear morphological and biological differences.
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Evaluation of genetic diversity by molecular markers in Indian gooseberry (Phyllanthus emblica)
(2025-01-01)
Poeaim, S.
;
Sangsoy, V.
;
Tangthirasunun, N.
The genetic diversity and relationships among 16 cultivars of Indian gooseberry (Phyllanthus emblica) using Sequence-Related Amplified Polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) markers were investigated. The cultivars were collected from the Phrae Horticultural Research Center, Thailand. For SRAP analysis, 30 primer combinations were initially tested, comprising five forward and six reverse primers. Seven primer combinations consistently generated well-defined bands and were selected for genetic profiling. These SRAP primers amplified 172 fragments, with 145 (84.30%) polymorphic. For RAPD analysis, 50 primers were evaluated, and eight primers consistently produced clear bands of genetic profiling. These RAPD primers amplified 215 fragments, with 188 (87.44%) polymorphic. The genetic relationships among the cultivars were assessed using a dendrogram constructed with NTSYS-pc (version 2.1X) based on the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The genetic similarity coefficients ranged from 0.66 to 0.90 for SRAP and from 0.58 to 0.89 for RAPD markers, indicating a high level of genetic diversity among the cultivars.
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Genetic characterization of cassava (Manihot esculenta Crantz) cultivars using ISSR molecular marker
(2024-05-01)
Poeaim, S.
;
Sansanee, S.
;
Chantaraprasit, T.
;
Chareonsap, P. P.
;
Tangthirasunun, N.
The genetic diversity of 22 cassava varieties using the inter-simple sequence repeat (ISSR) molecular marker was investigated. Genetic similarity was derived from the matrix of simple similarity coefficients using the unweighted pair group method with arithmetic average (UPGMA). The genetic similarity coefficient ranged from 0.73 to 0.94. The similarity coefficient at 0.73, cluster analysis was clustered to be 22 cassava varieties into two significant groups. The first group was the sweet variety of cassava used for consumption, consisting of Rayong2, Hana-tee and variegated leaves used for decoration. Another group, the bitter variety of cassava used for industry, consisted of Rayong1, Rayong3, Rayong5, Rayong7, Rayong9, Rayong11, Rayong15, Rayong60, Rayong72, Rayong86-13, Rayong90, Kasetsart50, Kasetsart72, Huay Bong60, Huay Bong80, Huay Bong90, Pirun1, Pirun2 and unknown. The sweet and bitter variety contained different levels of cyanide content. So, the ISSR marker might be able characterize two major cassava varieties.
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Isolation, screening wood rot fungi from the tropical forest of Thailand and their lignocellulolytic enzyme production under solid-state fermentation using agricultural waste as substrate
(2025-01-01)
Luong, Thi Thu Huong
;
Silar, Philippe
;
Poeaim, Supattra
;
Tangthirasunun, Narumon
The present study aimed to discover good lignocellulolytic enzyme (LCE) producers from Thailand’s tropical forest and then examine their multiple LCE production (including carboxymethyl cellulase (CMCase), xylanase, and laccase) using agricultural wastes as substrate. The total collection was 50 fungi, mainly from the Polyporales, Agaricales, and Xylariales orders. During primary screening by qualitative method and secondary screening by quantitative method, two potential fungi were proposed for multiple LCE production, including Auricularia auricula-judae 088 and Pseudolagarobasidium acaciicola TDW-48. Under solid-state fermentation (SSF) using agricultural wastes as substrates, P. acaciicola TDW-48 performed as a good producer that highly secreted simultaneous CMCase, xylanase, and laccase. In the next stage, the simplex lattice mixture design assessed the interaction of agricultural waste substrates and their effects on P. acaciicola TDW-48’s enzyme production. The results indicated that agricultural waste has different influences on CMCase, xylanase, and laccase production: orange peel showed a positive effect on both CMCase and xylanase activity, but a negative effect on laccase. In contrast, wheat bran positively influenced laccase, while it limited CMCase and xylanase. However, the combination of these substrates in the mixture showed synergic effects and improved enzyme activity. Through numerical optimization, a ternary mixture of wheat bran (1.27 g), orange peel (1.53 g), and rice husk (0.2 g) was identified as the most appropriate formulation for simultaneous multiple LCE production, reaching 20.96 U/g substrate for CMCase, 23.94 U/g substrate for xylanase, and 27.55 U/g substrate for laccase. These results provided a promising candidate for LCE production with high applicability in lignocellulose bioconversion and successfully demonstrated the relationship between the agricultural waste substrate and multiple LCE production that supported the enzyme production following the environmentally friendly and economical approach.
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Diversity of Sordariales Fungi: Identification of Seven New Species of Naviculisporaceae Through Morphological Analyses and Genome Sequencing
(2025-12-01)
Tangthirasunun, Narumon
;
Gautier, Valérie
;
Lalanne, Christophe
;
Bonometti, Lucas
;
Cros-Arteil, Sandrine
Thanks to next-generation sequencing (NGS) technologies, the diversity of fungi can now be investigated through the analysis of their genome sequences. Naviculisporaceae is a family within the Sordariales, whose diversity is not well-known, with only one genome sequence published for this family. Here, we report on the isolation and cultivation of 20 new strains of Naviculisporaceae. Their genome sequences, as well as those of the five commercially available strains, were determined, thus providing complete genome sequences for 25 new Naviculisporaceae strains. Species delimitation was conducted using a combination of (1) ITS + LSU phylogenetic analysis of the new isolates along with other known species of the family, (2) comparisons between DNA barcode sequences of the new strains with those of the known species, and (3) average genome-wide nucleotide identity calculation. We built a phylogenomic tree and studied the organization of the mating-type locus. In vitro fruiting was obtained for 16 strains, enabling the definition of seven new species, namely Pseudorhypophila gallica, Pseudorhypophila guyanensis Rhypophila alpibus, Rhypophila brasiliensis, Rhypophila camarguensis, Rhypophila reunionensis and Rhypophila thailandica, as well as two new combinations, namely Pseudorhypophila latipes and Pseudorhypophila oryzae. Eight strains for which in vitro fruiting was not obtained may belong to additional new species. These results expand the known diversity of the Naviculisporaceae and greatly enlarge the genomic data available for the family.
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Tangthirasunun, Narumon

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