Poeaim, Supattra

Preferred name

Poeaim, Supattra

Alternative Name

Poeaim, S.

Poeaim, Supattar

Main Affiliation

School of Science

supattra.poe@kmitl.ac.th

Now showing 1 - 10 of 28

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Investigation of Biodegradation and Biocompatibility of Chitosan–Bacterial Cellulose Composite Scaffold for Bone Tissue Engineering Applications
(2025-05-01)
Yodsanga, Somchai
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Poeaim, Supattra
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Chantarangsu, Soranun
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Swasdison, Somporn
Developing scaffolds with a three-dimensional porous structure and adequate mechanical properties remains a key challenge in tissue engineering of bone. These scaffolds must be biocompatible and biodegradable to effectively support osteoblastic cell attachment, metabolic activity, and differentiation. This study successfully fabricated a chitosan–bacterial cellulose (CS–BC) composite scaffold using the solvent casting/particle leaching (SCPL) technique, with NaOH/urea solution and sodium chloride crystals as the porogen. The scaffold exhibited a well-distributed porous network with pore sizes ranging from 300 to 500 µm. Biodegradation tests in PBS containing lysozyme revealed a continuous degradation process, while in vitro studies with MC3T3-E1 cells (pre-osteoblastic mouse cell line) demonstrated excellent cell attachment, as observed through SEM imaging. The scaffold also promoted increased metabolic activity (OD values) in the MTT assay, and enhanced alkaline phosphatase (ALP) activity and upregulated expression of osteogenic-related genes. These findings suggest that the CS–BC composite scaffold, fabricated using the SCPL method, holds great potential as a candidate for bone tissue engineering applications.
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Four new species of genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae) from Southern Thailand
(2025-01-01)
Seedee, Kunya
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Dumrongrojwattana, Pongrat
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Poeaim, Supattra
This study explores the diversity of microsnails inhabiting limestone caves in Southern Thailand. It describes four new species of the genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae): Acmella krueangensis sp. nov. from Ranong Province, A. thamsingensis sp. nov. and A. changphueakensis sp. nov. from Chumphon Province, and A. kanchanaditensis sp. nov. from Surat Thani Province. These species are distinguished by shell morphology, particularly the number of striae on the last whorl and the protoconch sculpture. Phylogenetic analyses based on mitochondrial cytochrome c oxidase subunit I (COI) gene sequences further support their distinctiveness and clarify their taxonomic placement. The results enhance the understanding of Acmella diversity in Thailand and shed light on biogeographical patterns found in this genus and the adaptations needed to survive under the conditions of cave systems.
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Plant regeneration of Bauhinia purpurea by tissue culture technique
(2024-05-01)
Saparam, W.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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Chemosensory-Driven Foraging and Nocturnal Activity in the Freshwater Snail Rivomarginella morrisoni (Gastropoda, Marginellidae): A Laboratory-Based Study
(2025-12-01)
Subpayakom, Navapong
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Dumrongrojwattana, Pongrat
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Poeaim, Supattra
Rivomarginella morrisoni is a freshwater snail endemic to Thailand, yet its behavioral ecology remains poorly understood. This study described the feeding behavior of R. morrisoni, focusing on its foraging activity, behavioral patterns, and food detection mechanisms under laboratory conditions using specimens collected from four river basins in central Thailand. Daily monitoring revealed nocturnal emergence, peaking between 21:00 and 22:00 h, with stable rhythms established 72 h post-feeding. Feeding trials revealed a preference for aged shrimp over fresh or decayed ones. Behavioral observations confirmed that food localization in R. morrisoni was mediated by chemical cues. Light–dark tests indicated a slight tendency toward darkness, but no significant phototactic response was observed. These findings suggest that R. morrisoni is a generalist scavenger with chemosensory-driven foraging and nocturnal activity. Its apparent sensitivity to habitat disturbance underscores the relevance of behavioral studies for informing future conservation and captive breeding efforts.
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Identification of SRAP and AFLP molecular markers associated with fruit traits in santol (Sandoricum koetjape)
(2024-07-01)
Pedklang, N.
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Poeaim, S.
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Sabpayakom, N.
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Vanijajiva, O.
The genetic diversity of santol (Sandoricum koetjape) using sequence-related amplified polymorphism (SRAP) and amplified fragment length polymorphism (AFLP) markers was investigated. Both molecular markers showed associations between fruit quality traits of economic importance, such as fruit weight and sweetness. The SRAP results revealed 128 amplified bands across nine primer pairs, indicating a polymorphism rate of 41.41%. Moreover, utilizing a genetic similarity coefficient of 0.85, santol cultivars were categorized into two major groups. Additionally, the AFLP analysis identified 171 amplified bands from seven primer pairs with a genetic similarity coefficient of 0.79. Consequently, santol cultivars were further classified into three distinct groups based on these findings. When combining the SRAP and AFLP results, a total of 299 amplified bands were analyzed, resulting in the separation of santol cultivars into four groups. These findings demonstrated that using SRAP and AFLP, as well as their combined results, could elucidate the genetic diversity within santol cultivars and may contribute to their classification.
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Influence of leaf type, genotype, and location on bioactivities and assessment of genetic diversity in teak (Tectona grandis L. f.)
(2026-03-01)
Poeaim, S.
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Phonmakham, J.
Marked variation in biological activities was observed among teak (Tectona grandis L. f.) leaf extracts as influenced by leaf type, tree genotype, and planting location. Fresh leaves generally exhibited stronger antibacterial activity than fallen leaves, with significant differences detected among plus trees originating from Chiang Mai, Phrae, Sukhothai, Lampang, and Khon Kaen and grown at the Thongphaphum and Phitsanulok Silviculture Research Stations. Among all samples, the Phrae plus tree cultivated at Thongphaphum showed the highest antibacterial inhibition against the tested microorganisms. Methanol extracts demonstrated antibacterial, anti-tyrosinase, and anti-inflammatory activities, while sequential extraction of Phrae plus tree leaves using hexane and dichloromethane yielded fractions with potent antibacterial effects, indicating promising potential for the development of new antibacterial agents. Genetic analysis based on sequence-related amplified polymorphism (SRAP) markers revealed similarity coefficients ranging from 0.67 to 1.00, reflecting low to moderate genetic diversity among the studied germplasm, with some samples exhibiting nearly identical genetic profiles. Overall, the observed variation in biological activities was closely associated with both genetic background and environmental conditions, providing valuable information for teak germplasm selection and the utilization of leaf-derived bioactive compounds in product development.
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Study on optimization for in vitro propagation of Dalbergia oliveri by plant tissue culture
(2024-03-01)
Nonthasila, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
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Poeaim, S.
The experimental findings demonstrated that the highest proliferation frequency (100%), along with the maximum number of shoots per explant (3.33±0.52 shoots) and an average shoot length of 30.04±1.70 mm, were achieved in the Murashige and Skoog (MS) medium supplemented with 2.0 mg/l of 6-benzylaminopurine (BAP). Subsequently, upon reaching a height of 3 to 4 cm, the in vitro micro shoots were transferred to a ¼ MS medium enriched with 0.75 mg/l of indole-3-acetic acid (IAA). This particular regimen resulted in the highest number of roots per shootlet (3.50±0.58) with an average root length of 34.19±0.79 mm. The acclimatization phase of the plantlets to the soil was successful, demonstrating a commendable survival rate of 80%.
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Effects of NaOH/urea solution as a solvent and salt crystals as a porogen on the fabrication of porous composite scaffold of bacterial cellulose-chitosan for tissue engineering
(2024-03-01)
Yodsanga, S.
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Poeaim, S.
A bacterial cellulose-chitosan composite scaffold fabricated through solvent casting-particular leaching method using NaOH/urea solution as a solvent and salt crystals as a porogen revealed a three-dimensional structure with the high porosity investigated by scanning electron microscope. The average porous size of the composite scaffold was between 300 and 500 um. The composite scaffold exhibited high water uptake, indicating to enhance water absorption capacity. The compressive test showed that the composite scaffold had good mechanical strength. Fourier-transform infrared spectroscopy analysis confirmed that bacterial cellulose and chitosan were found to be the main components of the composite scaffold. These results indicated that bacterial cellulose-chitosan composite scaffold could be used for application in tissue engineering.
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Evaluation of genetic diversity by molecular markers in Indian gooseberry (Phyllanthus emblica)
(2025-01-01)
Poeaim, S.
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Sangsoy, V.
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Tangthirasunun, N.
The genetic diversity and relationships among 16 cultivars of Indian gooseberry (Phyllanthus emblica) using Sequence-Related Amplified Polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) markers were investigated. The cultivars were collected from the Phrae Horticultural Research Center, Thailand. For SRAP analysis, 30 primer combinations were initially tested, comprising five forward and six reverse primers. Seven primer combinations consistently generated well-defined bands and were selected for genetic profiling. These SRAP primers amplified 172 fragments, with 145 (84.30%) polymorphic. For RAPD analysis, 50 primers were evaluated, and eight primers consistently produced clear bands of genetic profiling. These RAPD primers amplified 215 fragments, with 188 (87.44%) polymorphic. The genetic relationships among the cultivars were assessed using a dendrogram constructed with NTSYS-pc (version 2.1X) based on the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The genetic similarity coefficients ranged from 0.66 to 0.90 for SRAP and from 0.58 to 0.89 for RAPD markers, indicating a high level of genetic diversity among the cultivars.
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Efficient rice straw saccharification by enzyme extract from Pseudolagarobasidium acaciicola TDW-48 and recycling its solid residue as a green and novel support for laccase immobilization
(2025-02-01)
Luong, Thi Thu Huong
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Poeaim, Supattra
This study aimed to provide a novel and feasible approach for rice straw bioconversion regarding the combination of enzymatic saccharification and recycling of its solid residue for enzyme immobilization. Firstly, rice straw was saccharified by Pseudolagarobasidium acaciicola TDW-48's enzyme extract and then optimized to improve its performance. Next, the enzymatic-degraded rice straw (a solid residue from saccharification) was recycled as an enzyme support for laccase immobilization. The result indicates that the rice straw saccharification by P. acaciicola TDW-48's enzyme extract was a practical process for biofuel and other value-added product production. It reached 13.4 g/L of reducing sugar concentration under optimum conditions: 12 h of incubation time, 43.22 °C of temperature, pH of 4.5 and substrate loading of 0.48 g. In the next regard, enzymatic-degraded rice straw under adsorption-crosslinking mode was a promising novel support for laccase immobilization. This process achieved 1.24 U/gsupport of immobilized laccase activity and 88.3% in immobilization yield. The immobilized laccase could maintain 96.71% activity after 4 cycles and 53% activity after 10 cycles with ABTS substrate, and its stability was enhanced compared with free form. In addition, the immobilized laccase could decolorize 0.14 mg of bromophenol blue after 3 h and retain 53% relative efficiency after 6 cycles under biocatalyst bag form. This study encouraged the recycling of solid residue from rice straw saccharification, improving rice straw bioconversion. Moreover, it provided green, novel and economic support for enzyme immobilization with high applicability and simplicity.

Poeaim, Supattra

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