Poeaim, Supattra

Preferred name

Poeaim, Supattra

Alternative Name

Poeaim, S.

Poeaim, Supattar

Main Affiliation

School of Science

supattra.poe@kmitl.ac.th

Now showing 1 - 10 of 18

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Investigation of Biodegradation and Biocompatibility of Chitosan–Bacterial Cellulose Composite Scaffold for Bone Tissue Engineering Applications
(2025-05-01)
Yodsanga, Somchai
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Poeaim, Supattra
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Chantarangsu, Soranun
;
Swasdison, Somporn
Developing scaffolds with a three-dimensional porous structure and adequate mechanical properties remains a key challenge in tissue engineering of bone. These scaffolds must be biocompatible and biodegradable to effectively support osteoblastic cell attachment, metabolic activity, and differentiation. This study successfully fabricated a chitosan–bacterial cellulose (CS–BC) composite scaffold using the solvent casting/particle leaching (SCPL) technique, with NaOH/urea solution and sodium chloride crystals as the porogen. The scaffold exhibited a well-distributed porous network with pore sizes ranging from 300 to 500 µm. Biodegradation tests in PBS containing lysozyme revealed a continuous degradation process, while in vitro studies with MC3T3-E1 cells (pre-osteoblastic mouse cell line) demonstrated excellent cell attachment, as observed through SEM imaging. The scaffold also promoted increased metabolic activity (OD values) in the MTT assay, and enhanced alkaline phosphatase (ALP) activity and upregulated expression of osteogenic-related genes. These findings suggest that the CS–BC composite scaffold, fabricated using the SCPL method, holds great potential as a candidate for bone tissue engineering applications.
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Four new species of genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae) from Southern Thailand
(2025-01-01)
Seedee, Kunya
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Dumrongrojwattana, Pongrat
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Poeaim, Supattra
This study explores the diversity of microsnails inhabiting limestone caves in Southern Thailand. It describes four new species of the genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae): Acmella krueangensis sp. nov. from Ranong Province, A. thamsingensis sp. nov. and A. changphueakensis sp. nov. from Chumphon Province, and A. kanchanaditensis sp. nov. from Surat Thani Province. These species are distinguished by shell morphology, particularly the number of striae on the last whorl and the protoconch sculpture. Phylogenetic analyses based on mitochondrial cytochrome c oxidase subunit I (COI) gene sequences further support their distinctiveness and clarify their taxonomic placement. The results enhance the understanding of Acmella diversity in Thailand and shed light on biogeographical patterns found in this genus and the adaptations needed to survive under the conditions of cave systems.
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Chemosensory-Driven Foraging and Nocturnal Activity in the Freshwater Snail Rivomarginella morrisoni (Gastropoda, Marginellidae): A Laboratory-Based Study
(2025-12-01)
Subpayakom, Navapong
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Dumrongrojwattana, Pongrat
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Poeaim, Supattra
Rivomarginella morrisoni is a freshwater snail endemic to Thailand, yet its behavioral ecology remains poorly understood. This study described the feeding behavior of R. morrisoni, focusing on its foraging activity, behavioral patterns, and food detection mechanisms under laboratory conditions using specimens collected from four river basins in central Thailand. Daily monitoring revealed nocturnal emergence, peaking between 21:00 and 22:00 h, with stable rhythms established 72 h post-feeding. Feeding trials revealed a preference for aged shrimp over fresh or decayed ones. Behavioral observations confirmed that food localization in R. morrisoni was mediated by chemical cues. Light–dark tests indicated a slight tendency toward darkness, but no significant phototactic response was observed. These findings suggest that R. morrisoni is a generalist scavenger with chemosensory-driven foraging and nocturnal activity. Its apparent sensitivity to habitat disturbance underscores the relevance of behavioral studies for informing future conservation and captive breeding efforts.
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Influence of leaf type, genotype, and location on bioactivities and assessment of genetic diversity in teak (Tectona grandis L. f.)
(2026-03-01)
Poeaim, S.
;
Phonmakham, J.
Marked variation in biological activities was observed among teak (Tectona grandis L. f.) leaf extracts as influenced by leaf type, tree genotype, and planting location. Fresh leaves generally exhibited stronger antibacterial activity than fallen leaves, with significant differences detected among plus trees originating from Chiang Mai, Phrae, Sukhothai, Lampang, and Khon Kaen and grown at the Thongphaphum and Phitsanulok Silviculture Research Stations. Among all samples, the Phrae plus tree cultivated at Thongphaphum showed the highest antibacterial inhibition against the tested microorganisms. Methanol extracts demonstrated antibacterial, anti-tyrosinase, and anti-inflammatory activities, while sequential extraction of Phrae plus tree leaves using hexane and dichloromethane yielded fractions with potent antibacterial effects, indicating promising potential for the development of new antibacterial agents. Genetic analysis based on sequence-related amplified polymorphism (SRAP) markers revealed similarity coefficients ranging from 0.67 to 1.00, reflecting low to moderate genetic diversity among the studied germplasm, with some samples exhibiting nearly identical genetic profiles. Overall, the observed variation in biological activities was closely associated with both genetic background and environmental conditions, providing valuable information for teak germplasm selection and the utilization of leaf-derived bioactive compounds in product development.
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Evaluation of genetic diversity by molecular markers in Indian gooseberry (Phyllanthus emblica)
(2025-01-01)
Poeaim, S.
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Sangsoy, V.
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Tangthirasunun, N.
The genetic diversity and relationships among 16 cultivars of Indian gooseberry (Phyllanthus emblica) using Sequence-Related Amplified Polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) markers were investigated. The cultivars were collected from the Phrae Horticultural Research Center, Thailand. For SRAP analysis, 30 primer combinations were initially tested, comprising five forward and six reverse primers. Seven primer combinations consistently generated well-defined bands and were selected for genetic profiling. These SRAP primers amplified 172 fragments, with 145 (84.30%) polymorphic. For RAPD analysis, 50 primers were evaluated, and eight primers consistently produced clear bands of genetic profiling. These RAPD primers amplified 215 fragments, with 188 (87.44%) polymorphic. The genetic relationships among the cultivars were assessed using a dendrogram constructed with NTSYS-pc (version 2.1X) based on the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The genetic similarity coefficients ranged from 0.66 to 0.90 for SRAP and from 0.58 to 0.89 for RAPD markers, indicating a high level of genetic diversity among the cultivars.
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Efficient rice straw saccharification by enzyme extract from Pseudolagarobasidium acaciicola TDW-48 and recycling its solid residue as a green and novel support for laccase immobilization
(2025-02-01)
Luong, Thi Thu Huong
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Poeaim, Supattra
This study aimed to provide a novel and feasible approach for rice straw bioconversion regarding the combination of enzymatic saccharification and recycling of its solid residue for enzyme immobilization. Firstly, rice straw was saccharified by Pseudolagarobasidium acaciicola TDW-48's enzyme extract and then optimized to improve its performance. Next, the enzymatic-degraded rice straw (a solid residue from saccharification) was recycled as an enzyme support for laccase immobilization. The result indicates that the rice straw saccharification by P. acaciicola TDW-48's enzyme extract was a practical process for biofuel and other value-added product production. It reached 13.4 g/L of reducing sugar concentration under optimum conditions: 12 h of incubation time, 43.22 °C of temperature, pH of 4.5 and substrate loading of 0.48 g. In the next regard, enzymatic-degraded rice straw under adsorption-crosslinking mode was a promising novel support for laccase immobilization. This process achieved 1.24 U/gsupport of immobilized laccase activity and 88.3% in immobilization yield. The immobilized laccase could maintain 96.71% activity after 4 cycles and 53% activity after 10 cycles with ABTS substrate, and its stability was enhanced compared with free form. In addition, the immobilized laccase could decolorize 0.14 mg of bromophenol blue after 3 h and retain 53% relative efficiency after 6 cycles under biocatalyst bag form. This study encouraged the recycling of solid residue from rice straw saccharification, improving rice straw bioconversion. Moreover, it provided green, novel and economic support for enzyme immobilization with high applicability and simplicity.
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Isolation and Characterization of a Promising Lignocellulolytic Enzyme Producer Pseudolagarobasidium acaciicola SL3-03 from Mangrove Soil in Thailand
(2025-01-01)
Jadtanim, Chanaphon
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Luong, Thi Thu Huong
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Poeaim, Supattra
Lignocellulolytic enzymes isolation from mangrove-derived organisms has many industrial advantages due to their efficiency in dealing with extreme and challenging conditions, such as high temperatures and salt concentrations. This study aimed to isolate fungal enzyme producers from mangrove soil in Thailand to produce lignocellulolytic enzymes (carboxymethyl cellulase: CMCase, xylanase, and laccase) and to characterize these enzymes to support industrial applications. Forty-eight fungi were isolated from the mangrove samples, and their enzyme-producing capabilities were assessed using primary and secondary screening methods. The findings revealed that Pseudolagarobasidium acaciicola SL3-03 emerged as a promising producer of lignocellulolytic enzymes. It exhibited the ability to produce 1.345 U/mL of CMCase, 1.293 U/mL of xylanase, and 43.126 U/mL of laccase. Furthermore, the enzymatic characteristics of P. acaciicola SL3-03 were analyzed. The CMCase exhibited optimal activity at 50 °C and pH 5.5, the xylanase at 50 °C and pH 4.8, and the laccase at 55 °C and pH 5. Besides, the CMCase and xylanase from P. acaciicola SL3-03 expressed high halotolerance abilities that could maintain activity and stability under high salt concentrations (149% activity at 5 M NaCl). Future studies may focus on structural analysis of the enzymes to further characterize and identify their specific types. The results suggest that mangrove soil harbors significant potential for discovering proficient lignocellulolytic enzyme producers with desirable characteristics, which can be advantageous for industrial applications.
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Isolation, screening wood rot fungi from the tropical forest of Thailand and their lignocellulolytic enzyme production under solid-state fermentation using agricultural waste as substrate
(2025-01-01)
Luong, Thi Thu Huong
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Silar, Philippe
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Poeaim, Supattra
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Tangthirasunun, Narumon
The present study aimed to discover good lignocellulolytic enzyme (LCE) producers from Thailand’s tropical forest and then examine their multiple LCE production (including carboxymethyl cellulase (CMCase), xylanase, and laccase) using agricultural wastes as substrate. The total collection was 50 fungi, mainly from the Polyporales, Agaricales, and Xylariales orders. During primary screening by qualitative method and secondary screening by quantitative method, two potential fungi were proposed for multiple LCE production, including Auricularia auricula-judae 088 and Pseudolagarobasidium acaciicola TDW-48. Under solid-state fermentation (SSF) using agricultural wastes as substrates, P. acaciicola TDW-48 performed as a good producer that highly secreted simultaneous CMCase, xylanase, and laccase. In the next stage, the simplex lattice mixture design assessed the interaction of agricultural waste substrates and their effects on P. acaciicola TDW-48’s enzyme production. The results indicated that agricultural waste has different influences on CMCase, xylanase, and laccase production: orange peel showed a positive effect on both CMCase and xylanase activity, but a negative effect on laccase. In contrast, wheat bran positively influenced laccase, while it limited CMCase and xylanase. However, the combination of these substrates in the mixture showed synergic effects and improved enzyme activity. Through numerical optimization, a ternary mixture of wheat bran (1.27 g), orange peel (1.53 g), and rice husk (0.2 g) was identified as the most appropriate formulation for simultaneous multiple LCE production, reaching 20.96 U/g substrate for CMCase, 23.94 U/g substrate for xylanase, and 27.55 U/g substrate for laccase. These results provided a promising candidate for LCE production with high applicability in lignocellulose bioconversion and successfully demonstrated the relationship between the agricultural waste substrate and multiple LCE production that supported the enzyme production following the environmentally friendly and economical approach.
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Study on the regeneration of sausage tree (Kigelia africana) by tissue culture
(2026-05-01)
Poeaim, S.
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Poeaim, A.
This study investigated the regeneration of sausage tree (Kigelia africana) using tissue culture techniques, focusing on callus induction, shoot regeneration from seeds, and root induction from shoot explants. Seeds were cultured on solid MS medium supplemented with various concentrations of BA (0.5, 1, 2, 3, and 5 mg L⁻¹) for 10 weeks. Callus formation was observed at all BA concentrations, with the largest mean callus volume (5214.17 mm³) and highest induction rate (80%) achieved at 5 mg L⁻¹. Shoot regeneration occurred across all BA treatments, with the highest number of shoots (45 shoots; 86.54%) obtained on medium containing 1 mg L⁻¹ BA. Root induction was studied using shoot segments derived from seed-induced shoots cultured on MS medium supplemented with IAA, NAA, or IBA for 4 weeks. IAA at 0.5 mg L⁻¹ induced roots in 33.33% of explants with an average root length of 6.68 mm. NAA at 2 mg L⁻¹ promoted the highest root induction (70.83%), though roots were shorter, while 1 mg L⁻¹ NAA produced fewer roots but with greater length (7.00 mm). IBA at 3 mg L⁻¹ induced rooting in 33.33% of explants with an average root length of 3.78 mm and also stimulated callus formation, with the largest callus volume observed at 2 mg L⁻¹. These results demonstrate that BA is effective for callus and shoot induction, whereas IAA, NAA, and IBA promote root formation, with distinct responses at different concentrations. The optimized protocols provide a foundation for in vitro propagation, mass multiplication, and conservation of K. africana, with potential applications in biotechnology and secondary metabolite production.
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Three-phase partitioning (TPP) purification, characterization and dye decolorization application of laccase from Pseudolagarobasidium acaciicola TDW-48
(2026-02-01)
Luong, Thi Thu Huong
;
Poeaim, Supattra
This study focused on the efficient and economical purification of laccase from P. acaciicola TDW-48 and its application in dye decolorization. Firstly, the P. acaciicola TDW-48's laccase was purified by the three-phase partitioning (TPP) method, and then the artificial neural network-genetic algorithm (ANN-GA) optimization was used to improve the purification yield. The result indicated that the TPP method successfully purified P. acaciicola TDW-48's laccase with a high purification yield. After ANN-GA optimization, the strong interactive effects of the TPP parameters on purification yield were demonstrated by a high-accuracy ANN model (R-value of 0.99918 for all datasets). An optimum TPP system was developed, achieving 138.7 % activity recovery and 1.62-fold purity at a 57.82 % salt concentration, pH 5.75, and a t-butanol/enzyme ratio of 1.5. In the following, the enzyme characteristics and application potential of purified laccase were determined. The P. acaciicola TDW-48's laccase showed a molecular weight of 60.5 kDa and functioned optimally at pH 3 and 30 °C. The kinetic parameters implied high affinity and catalytic efficiency for the ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) substrate, with a low Km (37.9 μM) and a high Vmax (46.01 mM/min). Moreover, the purified laccase showed excellent potential for dye decolorization, with 44 % Congo red, 80 % bromophenol blue, and 58 % phenol red decolorized after 8 h of treatment.

Poeaim, Supattra

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