Phunpruch, Saranya

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Phunpruch, Saranya
Alternative Name
Phunpruch, S.
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saranya.ph@kmitl.ac.th

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    Item type:Publication,
    Simazine Enhances Dark Fermentative H2 Production by Unicellular Halotolerant Cyanobacterium Aphanothece halophytica
    (2022-07-15)
    Pansook, Sunisa
    ;
    Incharoensakdi, Aran
    ;
    Phunpruch, Saranya
    The halotolerant cyanobacterium Aphanothece halophytica is a potential H<inf>2</inf> producer that induces H<inf>2</inf> evolution under nitrogen deprivation. H<inf>2</inf> is mainly produced via the catabolism of stored glycogen under dark anaerobic condition. H<inf>2</inf> evolution is catalyzed by O<inf>2</inf>-sensitive bidirectional hydrogenase. The aim of this study was to improve H<inf>2</inf> production by A. halophytica using various kinds of inhibitors. Among all types of inhibitors, simazine efficiently promoted the highest H<inf>2</inf> production under dark conditions. High simazine concentration and long-term incubation resulted in a decrease in cell and chlorophyll concentrations. The optimal simazine concentration for H<inf>2</inf> production by A. halophytica was 25 µM. Simazine inhibited photosynthetic O<inf>2</inf> evolution but promoted dark respiration, resulting in a decrease in O<inf>2</inf> level. Hence, the bidirectional hydrogenase activity and H<inf>2</inf> production was increased. A. halophytica showed the highest H<inf>2</inf> production rate at 58.88 ± 0.22 µmol H<inf>2</inf> g<sup>−1</sup> dry weight h<sup>−1</sup> and H<inf>2</inf> accumulation at 356.21 ± 6.04 μmol H<inf>2</inf> g<sup>−1</sup> dry weight after treatment with 25 µM simazine under dark anaerobic condition for 2 and 24 h, respectively. This study demonstrates the potential of simazine for the enhancement of dark fermentative H<inf>2</inf> production by A. halophytica.