Phunpruch, Saranya

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Phunpruch, Saranya
Alternative Name
Phunpruch, S.
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Email
saranya.ph@kmitl.ac.th

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    LexA regulates the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 as a transcription activator
    (2005-11-01)
    Gutekunst, Kirstin
    ;
    Phunpruch, Saranya
    ;
    Schwarz, Christoph
    ;
    Schuchardt, Sven
    ;
    Schulz-Friedrich, Rüdiger
    The bidirectional NiFe-hydrogenase of Synechocystis sc. PCC 6803 is encoded by five genes (hoxEFUYH) which are transcribed as one unit. The transcription of the hox-operon is regulated by a promoter situated upstream of hoxE. The transcription start point was located at -168 by 5′Race. Several promoter probe vectors carrying different promoter fragments revealed two regions to be essential for the promoter activity. One is situated in the untranslated 5′leader region and the other is found -569 to -690 nucleotides upstream of the ATG. The region further upstream was shown to bind a protein. Even though an imperfect NtcA binding site was identified, NtcA did not bind to this region. The protein binding to the DNA was purified and found to be LexA by MALDI-TOF. The complete LexA and its DNA binding domain were overexpressed in Escherichia coli. Both were able to bind to two sites in the examined region in band-shift-assays. Accordingly, the hydrogenase activity of a LexA-depleted mutant was reduced. This is the first report on LexA acting not as a repressor but as a transcriptional activator. Furthermore, LexA is the first transcription factor identified so far for the expression of bidirectional hydrogenases in cyanobacteria. © 2005 The Authors. Journal compilation © 2005 Blackwell Publishing Ltd.
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    Nucleotide sequencing and transcriptional analysis of uptake hydrogenase genes in the filamentous N2-fixing cyanobacterium Anabaena siamensis
    (2006-12-01)
    Phunpruch, Saranya
    ;
    Baebprasert, Wipawee
    ;
    Thongpeng, Chamaporn
    ;
    Incharoensakdi, Aran
    The hupSL encoding uptake hydrogenase of the N<inf>2</inf>-fixing cyanobacterium Anabaena siamensis was cloned and sequenced. Anabaena siamensis hupS and hupL contained 963 and 1,596 bp, respectively. The predicted gene products for hupS and hupL consisted of 320 amino acids with a molecular mass of 35,436 Da and 531 amino acids with a molecular mass of 60,704 Da, respectively. Their deduced amino acid sequences showed higher than 90% and 88% similarity for HupS and HupL respectively, compared to those of other cyanobacteria including non N<inf>2</inf>-fixing cyanobacteria. The intergenic 195-bp sequence between hupS and hupL showed no homology to others among cyanobacteria but it still contained the repetitive sequence, which could form a hairpin structure commonly found in other N<inf>2</inf>-fixing cyanobacteria. RT-PCR analysis showed higher transcription level of hupS and hupL in Anabaena siamensis grown in BG11<inf>0</inf> under anaerobic conditions. Nickel exogenously added to the culture was also found to increase hupL transcript. © 2006 Springer Science+Business Media, Inc.
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    Distribution of hsp65 PCR-restriction enzyme analysis patterns among Mycobacterium avium complex isolates in Thailand
    (2006-10-01)
    Prammananan, Therdsak
    ;
    Phunpruch, Saranya
    ;
    Tingtoy, Nipa
    ;
    Srimuang, Somboon
    ;
    Chaiprasert, Angkana
    A total of 227 clinical Mycobacterium avium complex isolates from Thailand were differentiated into species and types by using PCR-restriction enzyme analysis of hsp65. The distribution of types showed the predominance of M. avium I (77%) in blood specimens, whereas M. intracellulare I was more commonly found in pulmonary specimens (44.2%). In addition, infections with M. avium were more likely to be found in younger adults (20 to 39 years old), while infections with M. intracellulare were more likely to be found in older adults (≥60 years old). Our results provide the useful epidemiological information that some particular types have more invasive and virulent characters than others. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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    Characterization of the antibacterial activity and probiotic properties of lactic acid bacteria isolated from raw fish and nham-plaa
    (2008-10-01)
    Nanasombat, Suree
    ;
    Phunpruch, Saranya
    ;
    Sriwong, Niracha
    ;
    Jaichalad, Thitirut
    ;
    Onnom, Waragul
    Isolates of lactic acid bacteria from fish fillets and nham-plaa were screened to select the most suitable strains for the formulation of starter cultures with probiotic properties. Using an agar spot test, 30 of the 180 isolates that were tested inhibited at least one of four indicator strains; Staphylococcus aureus TISTR 118, Pediococcus acidilactici TISTR 051, Lactobacillus bulgaricus TISTR 415 and Listeria monocytogenes DMST 11256. Two bacterial isolates, 13IS3 and 13IS4, from fish fillets were resistant to a low pH in lactic acid (pH 2.9-3.2) and in HCl (pH 2.2) and a high concentration of NaCl (6.0%) and bile salts (5.25%). These isolates were identified by morphological, biochemical and molecular analysis as Lactococcus lactis 13IS3 and Lactobacillus sakei 13IS4. The fermentation of nham-plaa model broth (NMB) by L. lactis 13IS3 seemed to be stimulated by 1.0% garlic extract and 0-1.5% NaCl at all temperatures of incubation. L. sakei 13IS4 fermented at a slower rate than L. lactis 13IS3, but fermented more rapidly in a 3.0%-garlic extract NMB with 0-1.5% NaCl at 30°C and in the same medium containing 1.0-1.5% NaCl at 35°C.
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    Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation detection
    (2008-01-01)
    Prammananan, T.
    ;
    Cheunoy, W.
    ;
    Taechamahapun, D.
    ;
    Yorsangsukkamol, J.
    ;
    Phunpruch, S.
    Since rifampicin resistance is a surrogate marker for multidrug-resistant Mycobacterium tuberculosis (MDRTB), the present study aimed to investigate rpoB mutations conferring rifampicin resistance in M. tuberculosis strains from Thailand, and to develop a rapid, inexpensive and simple PCR-based method for rapid detection of MDRTB. Overall, 267 M. tuberculosis isolates, including 143 MDRTB isolates, were investigated. Isolates of the Beijing strain predominated among the MDRTB isolates (79.1%), but accounted for only 45.5% of the susceptible isolates. Mutations in the rpoB gene were found most commonly at codons 531, 526 and 516 (58%, 25.2% and 9.1%, respectively). A multiplex allele-specific PCR was developed and tested with 216 clinical isolates. In comparison with the proportion method, the method showed 94.2% sensitivity and 100% specificity, and had a 100% positive predictive value and a 95% negative predictive value, which suggested that this method could be useful for screening for MDRTB, particularly in resource-limited countries. © 2008 European Society of Clinical Microbiology and Infectious Diseases.