Phunpruch, Saranya

Preferred name

Phunpruch, Saranya

Alternative Name

Phunpruch, S.

Main Affiliation

School of Science

saranya.ph@kmitl.ac.th

Now showing 1 - 10 of 25

Some of the metrics are blocked by your
consent settings
Morphological and genetic characterization of marine filamentous cyanobacterium Geitlerinema isolated from Thailand
(2018-04-01)
Tinpranee, Nichanan
;
Worananthakij, Worakrit
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Geitlerinema is a dominant benthic filamentous cyanobacterium found in the Gulf of Thailand and the Andaman Sea. We investigate the diversity of Geitlerinema strains isolated from Thailand using morphological and molecular characterization. No morphological differences were observed in the Geitlerinema isolates. Nucleotide sequencing and phylogenetic analyses of the 16S rDNA, the 16S–23S rRNA internal transcribed spacer (16S–23S ITS) and the cpcB-cpcA intergenic spacer (cpcB-cpcA IGS) showed that the marine Geitlerinema isolates belong to a single cluster that includes marine Geitlerinema sp. Flo1 and Geitlerinema sp. PCC7105 but is distinct from a type species, Geitlerinema splendidum and Geitlerinema strains which were recently transferred to the genus Anagnostidinema. From our results, it is confirmed that marine Geitlerinema differs from true freshwater Geitlerinema and is proposed to be a new genus in Oscillatoriales. In addition, using random amplification of polymorphic DNA (RAPD), the marine Geitlerinema isolates in this study could be classified into eight clades; however, this classification revealed no correlation with the geographic locations.
Some of the metrics are blocked by your
consent settings
Overexpression of eis without a mutation in promoter region of amikacin- and kanamycin-resistant Mycobacterium tuberculosis clinical strain
(2018-07-16)
Sowajassatakul, Angkanang
;
Prammananan, Therdsak
;
Chaiprasert, Angkana
;
Phunpruch, Saranya
Background: Aminoglycosides such as amikacin and kanamycin are effective injectable second-line drugs for treatment of multidrug-resistant tuberculosis. Molecular mechanisms underlying aminoglycoside resistance are not well understood. We have previously identified the amikacin- and kanamycin-resistant M. tuberculosis MT433 clinical strain, of which all known mutations related to resistance have not been found. Drug efflux pump is one of reported resistance mechanisms that might play a role in aminoglycoside resistance. Methods: The expression levels of sixteen putative efflux pump genes, including eis and one regulator gene, whiB7, of MT433 in the presence of kanamycin were determined using the reverse transcription-quantitative PCR method. The effects of upregulated genes on amikacin and kanamycin resistance were investigated by overexpression in M. tuberculosis H37Ra strain. Results: Upon kanamycin exposure, other than whiB7 and eis that were found extremely overexpressed, two drug efflux pump genes, namely Rv1877 and Rv2846c, showed specifically high-level of expression in M. tuberculosis MT433 strain. However, direct effect of overexpressed Rv1877 and Rv2846c on amikacin and kanamycin resistance could not be demonstrated in M. tuberculosis H37Ra overexpressed strain. Conclusions: Our finding demonstrated that overexpression of eis could occur without any mutations in the promoter region and be detectable in clinical isolate. This might be a consequence of overexpressed whiB7, resulting in amikacin and kanamycin resistance in M. tuberculosis MT433 strain.
Some of the metrics are blocked by your
consent settings
Effect of light intensity and light pattern on hydrogen production by unicellular green alga chlorella sp. LSD-W2
(2019-01-01)
Puangplub, Amornrat
;
Phunpruch, Saranya
Green microalgae can use solar energy and water to produce H2 via hydrogenase enzyme activity. The unicellular green alga Chlorella sp. LSD-W2 has been previously shown to produce high H2 under nitrogen deprivation. This research aimed to examine the effects of light intensity and light pattern on H2 production by Chlorella sp. LSD-W2 under nitrogen deprivation. The result showed that H<inf>2</inf> production rate was significantly enhanced when light intensities were increased. The cells could hardly produce H2 in the dark. The highest H2 production rate with 0.956 ± 0.015 mL L-1 h-1 was obtained in cells incubated in TAP-N medium in a 120-mL glass bottle under light intensity of 60 μmol photons m-2 s-1. H<inf>2</inf> production by cells incubated under light/dark or dark/light cycles was lower than that under continuous light illumination. In order to reduce O2 which is an inhibitor of hydrogenase enzyme, the PSII inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was added to the Chlorella sp. LSD-W2 cell cultures. It was found that O<inf>2</inf> was obviously decreased in cells treated with 10 μM DCMU. Unexpectedly, DCMU caused the reduction of H<inf>2</inf> production by Chlorella sp. LSD-W2.
Some of the metrics are blocked by your
consent settings
Screening cyanobacteria from marine coastal waters of Thailand for biohydrogen production
(2018-12-01)
Tinpranee, Nichanan
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Cyanobacteria are prokaryotic organisms capable of oxygenic photosynthesis. H <inf>2</inf> can be produced by cyanobacterial bidirectional hydrogenase, but mainly during anaerobic dark fermentation. Here, we screened H <inf>2</inf> producing cyanobacteria isolated from marine environments in Thailand and optimized physiological conditions for maximizing H <inf>2</inf> production of the selected isolate. Most of the 54 cyanobacterial strains isolated and purified from samples of seawater, stones, sand, and shells in the Gulf of Thailand and the Andaman Sea, southern part of Thailand produced H <inf>2</inf> when cells were incubated in nitrogen-deprived medium under dark/anaerobic condition. The filamentous non-heterocystous cyanobacterium Geitlerinema sp. RMK-SH10 gave the highest H <inf>2</inf> yield with highest H <inf>2</inf> production rate found in 7-day grown cells. Geitlerinema sp. RMK-SH10 showed maximum H <inf>2</inf> production rate of 0.271 ± 0.013 μmol H <inf>2</inf> mg −1 dry weight h −1 when incubated in NaNO <inf>3</inf> -free ASN III medium containing 0.2 M NaCl, 18.9 mmol C-atom of glucose L −1 , and 0.1 μM Ni 2+ . These results suggest that the marine filamentous cyanobacterium Geitlerinema sp. RMK-SH10 has high potential as a H <inf>2</inf> producer amenable for further improvement by genetic manipulation.
Some of the metrics are blocked by your
consent settings
Effect of pH on Biohydrogen Production in Green Alga Tetraspora sp. CU2551
(2017-01-01)
Maneeruttanarungroj, Cherdsak
;
Phunpruch, Saranya
The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in a high H<inf>2</inf> production yield, we further studied the enhancement of the H<inf>2</inf> production capacity by a substrate-level control to the key enzyme, Hydrogenase. This enzyme catalyzes the formation of biohydrogen gas from protons and electrons. Here, we investigated the effect of pH on the production rate which directly affected the flow of proton flux to the hydrogenase enzyme. The result revealed that pH of culture TAP medium was simply controlled by the use of HCl/NaOH solution over the pH range of 6.5 - 9.0 without any effect on the growth of algal cells. The H<inf>2</inf> production was observed in all ranges of our focusing pH with comparable to the control condition (pH 7.0 of TAP medium). In our best condition, an increase of the H<inf>2</inf> yield about 24% was found in cells cultivated in TAP medium adjusted the pH to 6.5 compared to the control condition. Our finding confirms the successful biohydrogen production enhancement by a substrate-level control for the key hydrogenase enzyme.
Some of the metrics are blocked by your
consent settings
Draft genome sequence of amikacin- and kanamycin-resistant Mycobacterium tuberculosis MT433 without rrs and eis mutations
(2015-01-01)
Sowajassatakul, Angkanang
;
Coker, Olabisi O.
;
Prammananan, Therdsak
;
Chaiprasert, Angkana
;
Phunpruch, Saranya
We announce the draft genome sequence of amikacin- and kanamycin-resistant Mycobacterium tuberculosis MT433, which has been previously described as the strain carrying an unknown resistance mechanism.
Some of the metrics are blocked by your
consent settings
Effect of Metal Cofactors of Key Enzymes on Biohydrogen Production by Nitrogen Fixing Cyanobacterium Anabaena siamensis TISIR 8012
(2017-01-01)
Taikhao, Samart
;
Phunpruch, Saranya
In N<inf>2</inf>-fixing cyanobacteria, three enzymes are involved in the H<inf>2</inf> metabolism. Nitrogenase catalyzes the N<inf>2</inf> fixation which produces H<inf>2</inf> as a by-product. The produced H<inf>2</inf> is taken up to protons and electrons by an activity of uptake hydrogenase. Reversible enzyme catalyzes both reactions of the H<inf>2</inf> evolution and the H<inf>2</inf> uptake. These enzymes are all metalloenzyme. The cyanobacterial nitrogenase normally requires molybdenum and iron as cofactors; however nitrogenase of few cyanobacterial species is dependent on vanadium. The cyanobacterial uptake and reversible hydrogenase requires nickel and iron as cofactors. This research aimed to investigate the effect of these metal cofactors on H<inf>2</inf> production and hydrogenase activity by N<inf>2</inf>-fixing cyanobacterium Anabaena siamensis TISTR 8012 isolated from rice paddle field in Thailand. The result showed that A. siamensis cells incubated in N-deprived BG11 medium (BG11<inf>0</inf>) gave clearly higher H<inf>2</inf> production rate and hydrogenase activity than those in normal BG11 medium. Under nitrogen deprivation, an increase of iron, nickel, and molybdenum concentrations obviously enhanced H<inf>2</inf> production rate. But only higher iron concentrations increased hydrogenase activity, indicating that the iron metal assisted in the function of reversible hydrogenase activity. In addition, vanadium seemed not to be a metal cofactor of key enzymes involving in H<inf>2</inf> production in A. siamensis. The optimal concentrations of iron, nickel and molybdenum ions for H<inf>2</inf> production rate by A. siamensis were 60 μM, 4 μM and 4 μM, respectively. The highest H<inf>2</inf> production rate of 0.057 μmolH<inf>2</inf> mg chl a-1 h-1 was observed in cells incubated in BG11<inf>0</inf> medium supplemented with 4 μM nickel ion.
Some of the metrics are blocked by your
consent settings
A role for 16S rRNA dimethyltransferase (ksgA) in intrinsic clarithromycin resistance in Mycobacterium tuberculosis
(2013-06-01)
Phunpruch, Saranya
;
Warit, Saradee
;
Suksamran, Rungaroon
;
Billamas, Pamaree
;
Jaitrong, Sarinya
The emergence of extensively drug-resistant tuberculosis (XDR-TB) makes the control of tuberculosis (TB) difficult. As a result, there is an urgent need to develop new anti-TB drugs. Alternatively, drugs that have already been used in humans as anti-infectives and later found to have antitubercular activity might be useful as anti-TB drugs, particularly against drug-resistant TB. Clarithromycin (CLR), a 14-membered macrolide and protein synthesis inhibitor, has potent activity against most mycobacterial infections, except Mycobacterium tuberculosis. Mycobacterium tuberculosis is naturally resistant to CLR [minimum inhibitory concentration (MIC) of 8-16 μg/mL] owing to the presence of inducible erm methylase (ErmMT). With a view to gaining further insight into the mechanisms of innate CLR resistance in M. tuberculosis, CLR-susceptible M. tuberculosis H37Rv mutants were generated by transposon mutagenesis. One mutant, designated as Tn-196, was further investigated and it was found that ksgA (Rv1010) was inactivated by the transposon. The ksgA gene encodes a 16S rRNA adenine dimethyltransferase that methylates A1518 and A1519 (Escherichia coli numbering) of 16S rRNA and plays an important role in ribosome biogenesis. Complementation of the Tn-196 mutant with a wild-type ksgA gene restored the resistant phenotype (MIC of 8-16 μg/mL), corroborating the association of ksgA with intrinsic CLR resistance in M. tuberculosis. © 2013 Elsevier B.V. and the International Society of Chemotherapy.
Some of the metrics are blocked by your
consent settings
Cellular lipid production of a heterotrophic bacterium isolated from poultry processing wastewater
(2014-01-01)
Ongmali, Rattiya
;
Phunpruch, Saranya
;
Thawornchaisit, Usarat
Cell growth and lipid production of a heterotrophic bacterium, R4.4 which accumulated the highest lipid content among 12 bacterial colonies being isolated from wastewater of a poultry processing plant in Thailand, were evaluated in this study. The culture was identified as Aeromonas sp. by 16S ribosomal DNA sequencing analysis.The highest lipid content was obtained when the cells were in the early stationary growth phase compared to the cells in the exponential and the late stationary phase. Over 50% of the fatty acid production by Aeromonas sp. KMITL-R4.4 were unsaturated fatty acids, including linoleic acid (C18:2, 43.2%), oleic acid (C18:1, 19.0%), and palmitoleic acid (C16:1, 8.2%). The culture had a preference for glucose and fructose as seen from the maximum biomass and lipid contents that were obtained. Among volatile fatty acid (VFA) species tested, acetic acid was the preferred substrate for lipid production but not favorable for cell growth. In addition, ammonium sulfate was found to be the best among nitrogen sources tested. The C/N ratio exerts significant impact on lipid production as seen from an increase of the lipid content from 10.8% to 18.2% by exposing the bacterial cells to a medium with lower nitrogen concentration (0.1g/l) and higher level of glucose (28 g/l).
Some of the metrics are blocked by your
consent settings
Screening and identification of lactic acid bacteria from raw seafoods and thai fermented seafood products for their potential use as starter cultures
(2012-01-01)
Nanasombat, Suree
;
Phunpruch, Saranya
;
Jaichalad, Thitirut
The number of lactic acid bacteria (LAB) was analyzed from 52 samples of raw seafoods (shrimp and mussel), and Thai fermented seafood products including fermented shrimp (kung-jom), mussel (hoi-dong), and fish (pla-jom). The viable LAB were 3.0×103 to 3.4×108 CFU/g. LAB were isolated and screened for their inhibitory activities against eight indicator bacteria by agar spot test. Among all selected LAB isolates, 52 isolates showed strong inhibitory activity. They were further characterized for their ability to resist hydrochloric acid, lactic acid, bile salts, and sodium chloride, and their ability to produce bacteriocins and amino acid decarboxylase. The selected LAB isolates, 1IS11 and 4IS17, were bacteriocin-producing strains, and showed no amino acid decarboxylase activity, which was suitable property for starter cultures. The isolate 1IS11 could resist both hydrochloric and lactic acid at the lowest pH of 2.0, while the isolate 4IS17 was able to tolerate hydrochloric and lactic acid at the lowest pH of 1.5 and 2.0, respectively. Both isolates could grow in MRS broth containing a high concentration of sodium chloride (10 %) and bile salts (1.5%). They were identified by morphological characterization, biochemical test, and 16S rDNA sequence analysis. The isolate 1IS11 was found to be Enterococcus faecium, whereas the isolate 4IS17 was Enterococcus faecalis.

Phunpruch, Saranya

Filters

Settings

Sort By

Results per page