Phunpruch, Saranya

Loading...
Thumbnail Image
Preferred name
Phunpruch, Saranya
Alternative Name
Phunpruch, S.
Main Affiliation
Email
saranya.ph@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Enhanced hydrogen production by green alga Scenedesmus obliquus TISTR 8546 under atmospheric air through potassium deprivation and cysteine supplementation
    (2026-03-01)
    Sereetrakul, Kodchaporn
    ;
    Taikhao, Samart
    ;
    Chinchusak, Nattanon
    ;
    Phunpruch, Saranya
    Hydrogen is a promising alternative energy carrier that can be produced by green microalgae. However, the key enzyme catalyzing this process, [FeFe]‑hydrogenase, is highly sensitive to O<inf>2</inf> generated during photosynthesis, which severely restricts its activity. This study aimed to screen the microalgal strain capable of producing high levels of H<inf>2</inf> in the presence of O<inf>2</inf> and to enhance H<inf>2</inf> production yields using O<inf>2</inf>-scavenging strategies to levels comparable to or exceeding those achieved under anaerobic conditions. Among the 24 strains tested, Scenedesmus obliquus TISTR 8546 demonstrated the highest H<inf>2</inf> production under both aerobic and anaerobic conditions. The O<inf>2</inf>I<inf>50</inf> of S. obliquus TISTR 8546 cells for H<inf>2</inf> evolution was 15.93 ± 0.24%. Potassium deprivation significantly enhanced H<inf>2</inf> production and hydrogenase activity by lowering O<inf>2</inf> levels through reduced photosynthetic O<inf>2</inf> evolution and increased dark respiration. Moreover, potassium deprivation promoted intracellular starch accumulation, providing reducing equivalents for H<inf>2</inf> generation. Cysteine supplementation further stimulated H<inf>2</inf> production by serving as a reducing agent. S. obliquus TISTR 8546 exhibited a maximum H<inf>2</inf> production rate of 22.92 ± 1.05 μmol H<inf>2</inf> mg Chl<sup>−1</sup> h<sup>−1</sup> and achieved a maximum cumulative H<inf>2</inf> production of 1153.78 ± 52.65 μmol H<inf>2</inf> mg Chl<sup>−1</sup> when incubated in potassium-deprived TAP medium supplemented with 0.1 mM cysteine under atmospheric air for 6 days. This rate was 23.6- and 2.7-fold higher than those obtained in TAP and TAP-K media, respectively. These findings demonstrate the potential of S. obliquus TISTR 8546 as a robust microalgal strain for sustainable H<inf>2</inf> production under atmospheric air, highlighting its promise for future industrial applications.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Physiological and Environmental Factors Influencing Hydrogen Production by Unicellular Green Alga Monoraphidium sp. KMITL-1
    (2025-10-01)
    Krutpan, Varanya
    ;
    Supakriangkrai, Thaninthorn
    ;
    Lohasupthawee, Pana
    ;
    Phunpruch, Saranya
    With the growing global energy demand and the urgent need to reduce carbon emissions, hydrogen (H<inf>2</inf>) has emerged as a promising clean energy carrier. Among various biological H<inf>2</inf> production, green algae present a sustainable and eco-friendly alternative due to their ability to produce H<inf>2</inf> via photobiological pathways. This study aimed to investigate H<inf>2</inf> production by unicellular green alga Monoraphidium sp. KMITL-1, isolated from hydroponic water at the Plant Tissue Culture Laboratory, King Mongkut’s Institute of Technology Ladkrabang. The taxonomic identity of the strain, belonging to the genus Monoraphidium within the Selenastraceae family, was confirmed through morphological observation and molecular characterization using 23S plastid rRNA gene sequencing. Various physiological and environmental parameters influencing H<inf>2</inf> production were evaluated, including cell age, cell density, nutrient deprivation, carbon source, pH, temperature, and light intensity. A 24-hour-old culture with an OD<inf>750</inf> of 0.8 exhibited a significant increase in H<inf>2</inf> production. The optimal medium was potassium-deprived Tris-acetate-phosphate (TAP-K) supplemented with glucose at a concentration of 350 mmol C-atom L<sup>-1</sup>. The ideal environmental conditions for H<inf>₂</inf> production were pH 7.2, a temperature of 30 °C, and a light intensity of 60 μmol photons m<sup>-2</sup> s<sup>-1</sup>. Under these optimized conditions, Monoraphidium sp. KMITL-1 achieved a maximum H<inf>2</inf> production rate of 67.976 ± 1.096 μmol H<inf>2</inf> mg Chl<sup>-1</sup> h<sup>-1</sup> and a cumulative H<inf>2</inf> yield of 3,190.436 ± 2.219 μmol H<inf>2</inf> mg Chl<sup>-1</sup> after 72 h of incubation. These results highlight the potential of Monoraphidium sp. KMITL-1 for large-scale biohydrogen production and its applicability in the development of sustainable energy technologies.