Phunpruch, Saranya

Preferred name

Phunpruch, Saranya

Alternative Name

Phunpruch, S.

Main Affiliation

School of Science

saranya.ph@kmitl.ac.th

Now showing 1 - 10 of 51

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Morphological and genetic characterization of marine filamentous cyanobacterium Geitlerinema isolated from Thailand
(2018-04-01)
Tinpranee, Nichanan
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Worananthakij, Worakrit
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Incharoensakdi, Aran
;
Phunpruch, Saranya
Geitlerinema is a dominant benthic filamentous cyanobacterium found in the Gulf of Thailand and the Andaman Sea. We investigate the diversity of Geitlerinema strains isolated from Thailand using morphological and molecular characterization. No morphological differences were observed in the Geitlerinema isolates. Nucleotide sequencing and phylogenetic analyses of the 16S rDNA, the 16S–23S rRNA internal transcribed spacer (16S–23S ITS) and the cpcB-cpcA intergenic spacer (cpcB-cpcA IGS) showed that the marine Geitlerinema isolates belong to a single cluster that includes marine Geitlerinema sp. Flo1 and Geitlerinema sp. PCC7105 but is distinct from a type species, Geitlerinema splendidum and Geitlerinema strains which were recently transferred to the genus Anagnostidinema. From our results, it is confirmed that marine Geitlerinema differs from true freshwater Geitlerinema and is proposed to be a new genus in Oscillatoriales. In addition, using random amplification of polymorphic DNA (RAPD), the marine Geitlerinema isolates in this study could be classified into eight clades; however, this classification revealed no correlation with the geographic locations.
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LexA regulates the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 as a transcription activator
(2005-11-01)
Gutekunst, Kirstin
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Phunpruch, Saranya
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Schwarz, Christoph
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Schuchardt, Sven
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Schulz-Friedrich, Rüdiger
The bidirectional NiFe-hydrogenase of Synechocystis sc. PCC 6803 is encoded by five genes (hoxEFUYH) which are transcribed as one unit. The transcription of the hox-operon is regulated by a promoter situated upstream of hoxE. The transcription start point was located at -168 by 5′Race. Several promoter probe vectors carrying different promoter fragments revealed two regions to be essential for the promoter activity. One is situated in the untranslated 5′leader region and the other is found -569 to -690 nucleotides upstream of the ATG. The region further upstream was shown to bind a protein. Even though an imperfect NtcA binding site was identified, NtcA did not bind to this region. The protein binding to the DNA was purified and found to be LexA by MALDI-TOF. The complete LexA and its DNA binding domain were overexpressed in Escherichia coli. Both were able to bind to two sites in the examined region in band-shift-assays. Accordingly, the hydrogenase activity of a LexA-depleted mutant was reduced. This is the first report on LexA acting not as a repressor but as a transcriptional activator. Furthermore, LexA is the first transcription factor identified so far for the expression of bidirectional hydrogenases in cyanobacteria. © 2005 The Authors. Journal compilation © 2005 Blackwell Publishing Ltd.
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Microwave-assisted biosynthesis of silver nanoparticles using two marine microalgal extracts and their antimycobacteriosis activity against bacteria isolated from Betta splendens
(2025-12-01)
Manklinniam, Piyapan
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Phunpruch, Saranya
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Sakulkalavek, Aparporn
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Sakdanuphab, Rachsak
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Worananthakij, Worakrit
This study explores the synthesis and antibacterial properties of silver nanoparticles (AgNPs) as a safer, eco-friendly alternative to traditional chemical treatments for bacterial infections. AgNPs were synthesized using aqueous extracts of marine microalgae, Isochrysis galbana and Chaetoceros calcitrans, via conventional and microwave-assisted methods, with the latter accelerating nanoparticle production. Extracts in ethanol, hexane, and acetone were tested, with the ethanolic extract of I. galbana showing the strongest antibacterial effects. The AgNPs exhibited broad-spectrum antibacterial activity against pathogens such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and fish pathogens like Aeromonas veronii. Microwave-assisted synthesis with ethanolic extracts resulted in the highest inhibition, particularly against fish and tuberculosis-related pathogens, including Mycobacterium marinum. Nanoparticle formation was confirmed using various characterization methods, including ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), which revealed crystalline structures. Transmission electron microscopy (TEM) analysis revealed that AgNPs varied in size, with an average diameter of less than 50 nm and all particles being smaller than 100 nm. This research demonstrates the potential of AgNPs as an effective alternative to antibiotics, offering targeted bacterial inhibition while reducing the risk of antibiotic resistance. This makes it a promising approach for treating bacterial infections in ornamental fish.
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Occurrence of Parasitic and Bacterial Pathogen in Ornamental and Wild Populations of Siamese Fighting Fish (Betta splendens) in a Region of Thailand
(2025-05-01)
Manklinniam, Piyapan
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Phunpruch, Saranya
;
Worananthakij, Worakrit
The diversity of fish parasites reflects the diversity of parasites in the water source, providing insights into the effects of pathogens and essential information about parasite-host relationships. Parasitic infections are valuable indicators of the aquatic ecosystem, influenced by various factors. This study aimed to investigate the prevalence and seasonality of parasite species and pathogenic bacteria in Siamese fighting fish (Betta splendens) over 12 months, involving sixty ornamental Siamese fighting fish collected from commercial sources and 81 wild Siamese fighting fish from different natural habitats. Parasite prevalence in ornamental Siamese fighting fish (25.00%) was lower than in the wild (34.57%). The protozoa parasite Trichodina was found in both Siamese fighting fish, with the highest prevalence recorded for Trichodina sp. in wild Siamese fighting fish. The winter season exhibited the highest parasitic prevalence, with a tremendous diversity of parasites found at each location, followed by the rainy and summer seasons. This study also reported the first finding of Henneguya sp. infection in Siamese fighting fish and on the body surface. The prevalence and seasonality of parasite genera were significant in the wild compared to ornamental Siamese fighting fish. Bacterial isolation was performed on internal organs, and isolates were identified using PCR techniques. Aeromonas veronii and Mycobacterium marinum were detected in ornamental Siamese fighting fish, while A. veronii was found in wild Siamese fighting fish. These findings indicate that infections in Siamese fighting fish display seasonal variation and are impacted by their ecology. This information is fundamental for managing the biodiversity of parasites in fish and preventing parasite infections in aquaculture.
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Overexpression of eis without a mutation in promoter region of amikacin- and kanamycin-resistant Mycobacterium tuberculosis clinical strain
(2018-07-16)
Sowajassatakul, Angkanang
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Prammananan, Therdsak
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Chaiprasert, Angkana
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Phunpruch, Saranya
Background: Aminoglycosides such as amikacin and kanamycin are effective injectable second-line drugs for treatment of multidrug-resistant tuberculosis. Molecular mechanisms underlying aminoglycoside resistance are not well understood. We have previously identified the amikacin- and kanamycin-resistant M. tuberculosis MT433 clinical strain, of which all known mutations related to resistance have not been found. Drug efflux pump is one of reported resistance mechanisms that might play a role in aminoglycoside resistance. Methods: The expression levels of sixteen putative efflux pump genes, including eis and one regulator gene, whiB7, of MT433 in the presence of kanamycin were determined using the reverse transcription-quantitative PCR method. The effects of upregulated genes on amikacin and kanamycin resistance were investigated by overexpression in M. tuberculosis H37Ra strain. Results: Upon kanamycin exposure, other than whiB7 and eis that were found extremely overexpressed, two drug efflux pump genes, namely Rv1877 and Rv2846c, showed specifically high-level of expression in M. tuberculosis MT433 strain. However, direct effect of overexpressed Rv1877 and Rv2846c on amikacin and kanamycin resistance could not be demonstrated in M. tuberculosis H37Ra overexpressed strain. Conclusions: Our finding demonstrated that overexpression of eis could occur without any mutations in the promoter region and be detectable in clinical isolate. This might be a consequence of overexpressed whiB7, resulting in amikacin and kanamycin resistance in M. tuberculosis MT433 strain.
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Effect of light intensity and light pattern on hydrogen production by unicellular green alga chlorella sp. LSD-W2
(2019-01-01)
Puangplub, Amornrat
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Phunpruch, Saranya
Green microalgae can use solar energy and water to produce H2 via hydrogenase enzyme activity. The unicellular green alga Chlorella sp. LSD-W2 has been previously shown to produce high H2 under nitrogen deprivation. This research aimed to examine the effects of light intensity and light pattern on H2 production by Chlorella sp. LSD-W2 under nitrogen deprivation. The result showed that H<inf>2</inf> production rate was significantly enhanced when light intensities were increased. The cells could hardly produce H2 in the dark. The highest H2 production rate with 0.956 ± 0.015 mL L-1 h-1 was obtained in cells incubated in TAP-N medium in a 120-mL glass bottle under light intensity of 60 μmol photons m-2 s-1. H<inf>2</inf> production by cells incubated under light/dark or dark/light cycles was lower than that under continuous light illumination. In order to reduce O2 which is an inhibitor of hydrogenase enzyme, the PSII inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was added to the Chlorella sp. LSD-W2 cell cultures. It was found that O<inf>2</inf> was obviously decreased in cells treated with 10 μM DCMU. Unexpectedly, DCMU caused the reduction of H<inf>2</inf> production by Chlorella sp. LSD-W2.
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Enhanced Hydrogen Production by the Halotolerant Cyanobacterium Aphanothece halophytica Through Bacterial Co-Cultivation
(2026-05-01)
Somsin, Chutikarn
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Chinchusak, Nattanon
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Incharoensakdi, Aran
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Phunpruch, Saranya
Hydrogen (H<inf>2</inf>) is a promising clean energy carrier with the potential to partially replace fossil fuels. Biological H<inf>2</inf> production using microorganisms offers an environmentally friendly alternative. The halotolerant cyanobacterium Aphanothece halophytica can produce H<inf>2</inf> under nitrogen-deprived and dark anaerobic conditions. In this study, a co-culture strategy was investigated to enhance H<inf>2</inf> production. Five bacterial strains were screened for their ability to improve H<inf>2</inf> production when co-cultivated with A. halophytica. Among them, Staphylococcus aureus significantly enhanced H<inf>2</inf> production, achieving a maximum rate of 11.11 ± 0.18 µmol H<inf>2</inf> g−1 dry weight h−1. Optimization of the bacterial partner revealed that S. aureus cells harvested at 12 h in the mid-logarithmic phase with an OD<inf>600</inf> of 4.0 were the most effective. An inoculum ratio of A. halophytica to S. aureus of 4:1 further enhanced H<inf>2</inf> production, increased bidirectional hydrogenase activity, and reduced O<inf>2</inf> accumulation. Under optimal conditions (0.945 mmol C-atom L−1 glucose, 0.25 M NaCl, pH 7.4, and 35 °C), the maximum H<inf>2</inf> production rate reached 132.49 ± 4.45 µmol H<inf>2</inf> g−1 dry weight h−1, approximately 5.5-fold higher than that under normal conditions. The co-culture achieved a cumulative H<inf>2</inf> yield of 3248.51 ± 88.11 µmol H<inf>2</inf> g−1 dry weight after 48 h.
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Factors affecting hydrogen production by unicellular green alga chlamydomonas reinhardtii cc-125
(2021-01-01)
Sereetrakul, Kodchaporn
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Phunpruch, Saranya
In this study, factors affecting H<inf>2</inf> production by the unicellular green alga Chlamydomonas reinhardtii CC-125 were investigated. It was found that the cell density, O<inf>2</inf> concentration and nutrient deprivation in media play important roles in H<inf>2</inf> production by C. reinhardtii CC-125. C. reinhardtii CC-125 at a cell age of 36 hours with an optical density at 750 nm of 0.8 shows the highest H<inf>2</inf> production rate. Similar to other microorganisms, higher O<inf>2</inf> concentrations decrease H<inf>2</inf> production. However, hydrogenase activity in C. reinhardtii CC-125 seems to show high O<inf>2</inf> tolerance, with an O<inf>2</inf>I<inf>50</inf> of 16.87 ± 0.81%. Under atmospheric air, maximum H<inf>2</inf> production of 100.90 ± 7.92 and 107.47 ± 3.72 mL L-1 was found in cells incubated in sulfur-deprived and sulfur-nitrogen-deprived media, respectively, after 7 days of incubation. The deprivation of both sulfur and nitrogen prolongs H<inf>2</inf> production in this algal strain.
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Synechococcus marine microalgae peptide: Melanogenesis inhibition in cellular and zebrafish models
(2024-08-01)
Srimongkol, Piroonporn
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Sangtanoo, Papassara
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Saisavoey, Tanatorn
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Puthong, Songchan
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Buakeaw, Anumart
In this study, the AILQSYSAGKTK (named AK-12) peptide, also known as AK-12, from the Synechococcus marine microalgae cell extract is examined to determine the mechanism by which tyrosinase inhibition takes place. According to the docking simulation, it is expected that the peptides bind and interact at the tyrosinase active site, with the potential to support the inhibition of tyrosinase. For testing, the required peptides were first synthesized, before the results revealed tyrosinase inhibitory activity for which the respective IC<inf>50</inf> values for mono- and di-phenolase activities were 489.71 ± 0.01 μM, and 765.57 ± 0.01 μM. The characteristics of the different competitive types were shown in a Lineweaver-Burk plot. For the treatment of B16F10 cells, peptide concentrations of 50–400 μM were selected, confirming the absence of cytotoxicity. When the peptide was introduced, both tyrosinase activity and the production of melanin were significantly inhibited. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then used to assess the suppression of melanin synthesis in MITF (microphthalmia-associated transcription factor, TYR (tyrosinase), TRP-1 (tyrosinase-related protein-1) and TRP-2 (tyrosinase-related protein-2). An in vivo toxicity assay was also carried out to evaluate zebrafish embryo cell death, revealing that AK-12 did not significantly affect cell death, while strong anti-melanogenic activity was observed for the concentration of 50 μM. From these findings it could be concluded that the peptide in question may offer potential in future hyperpigmentation treatments.
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Stable Nanoemulsion System Development Enabling the Topical Delivery of Synechococcus-Derived Peptides
(2026-03-01)
Keawbankrud, Wannisa
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Nakyai, Wongnapa
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Phunpruch, Saranya
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Sangtanoo, Papassara
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Karnchanatat, Aphichart
Marine microalgae including Synechococcus sp. VDW offer the potential to serve as a source of bioactive peptides offering valuable antioxidant and antimelanogenic qualities for use in the pharmaceutical and cosmetics industries. At present, however, the use of such peptides is challenging due to their poor physicochemical stability. This research therefore sought to achieve the production and characterization of a stable nanoemulsion system based upon the use of synthetic Synechococcus-derived peptides through the process of high-pressure homogenization (HPH). Preparation of the nanoemulsions involved the use of Tween-80 and caprylic/capric triglyceride at a pressure of 7,500 psi to perform homogenization for varying durations of 15, 30 and 45 min. Using the optimized formulation with 0.1% w/w peptide for a time of 45 min resulted in 122.16 nm droplets while the zeta potential was −80.09 mV and the PDI (polydispersity index) value was 0.13. Colloidal stability could be considered high, while physical stability under thermal cycling, centrifugation and freeze-thaw cycles was very good, with no phase separation. For all testing intervals, the viscosity and refractive index were stable. It can thus be argues that the HPH approach is suitable to produce peptide-loaded nanoemulsions offering good stability and useful physicochemical characteristics. The developed nanoemulsion system has been optimized to offer potential for applications involving the transdermal delivery of marine peptides in the cosmeceutical sector and for a range of dermal therapies.

Phunpruch, Saranya

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