Phunpruch, Saranya

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Phunpruch, Saranya
Alternative Name
Phunpruch, S.
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Email
saranya.ph@kmitl.ac.th

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    Physiological and Environmental Factors Influencing Hydrogen Production by Unicellular Green Alga Monoraphidium sp. KMITL-1
    (2025-10-01)
    Krutpan, Varanya
    ;
    Supakriangkrai, Thaninthorn
    ;
    Lohasupthawee, Pana
    ;
    Phunpruch, Saranya
    With the growing global energy demand and the urgent need to reduce carbon emissions, hydrogen (H<inf>2</inf>) has emerged as a promising clean energy carrier. Among various biological H<inf>2</inf> production, green algae present a sustainable and eco-friendly alternative due to their ability to produce H<inf>2</inf> via photobiological pathways. This study aimed to investigate H<inf>2</inf> production by unicellular green alga Monoraphidium sp. KMITL-1, isolated from hydroponic water at the Plant Tissue Culture Laboratory, King Mongkut’s Institute of Technology Ladkrabang. The taxonomic identity of the strain, belonging to the genus Monoraphidium within the Selenastraceae family, was confirmed through morphological observation and molecular characterization using 23S plastid rRNA gene sequencing. Various physiological and environmental parameters influencing H<inf>2</inf> production were evaluated, including cell age, cell density, nutrient deprivation, carbon source, pH, temperature, and light intensity. A 24-hour-old culture with an OD<inf>750</inf> of 0.8 exhibited a significant increase in H<inf>2</inf> production. The optimal medium was potassium-deprived Tris-acetate-phosphate (TAP-K) supplemented with glucose at a concentration of 350 mmol C-atom L<sup>-1</sup>. The ideal environmental conditions for H<inf>₂</inf> production were pH 7.2, a temperature of 30 °C, and a light intensity of 60 μmol photons m<sup>-2</sup> s<sup>-1</sup>. Under these optimized conditions, Monoraphidium sp. KMITL-1 achieved a maximum H<inf>2</inf> production rate of 67.976 ± 1.096 μmol H<inf>2</inf> mg Chl<sup>-1</sup> h<sup>-1</sup> and a cumulative H<inf>2</inf> yield of 3,190.436 ± 2.219 μmol H<inf>2</inf> mg Chl<sup>-1</sup> after 72 h of incubation. These results highlight the potential of Monoraphidium sp. KMITL-1 for large-scale biohydrogen production and its applicability in the development of sustainable energy technologies.
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    Effect of cell density and nutrient deprivation on hydrogen production by unicellular green alga Scenedesmus sp. KMITL-OVG1
    (2019-01-01)
    Warichanan, Kittiphat
    ;
    Phunpruch, Saranya
    Hydrogen is considered as one of the energy carriers for the near future. H2 production by green algae is catalyzed by hydrogenase activity using electrons from photosynthetic process under the light and from accumulated carbohydrate catabolism in the dark. This research aimed to investigate the effect of cell density and nutrient deprivation on H<inf>2</inf> production by Scenedesmus sp. KMITL-OVG1 isolated in Thailand. The result showed that cell culture with the optical density at 750 nm of 0.8 gave the highest H2 production rate. Interestingly, the highest H2 production rate of 1.957 ± 0.100 mL L<sup>-1</sup> h<sup>-1</sup> and hydrogenase activity of 0.031 ± 0.001 ml L<sup>-1</sup> min<sup>-1</sup> were found in cells incubated under potassium deprivation. H<inf>2</inf> production rate was approximately 3 folds higher than that of cells incubated in normal TAP medium. The increased H2 production rate and hydrogenase activity might be involved in the reduction of starch accumulation. Moreover, the deprivation of potassium combined with other nutrients did not enhance H<inf>2</inf> production rate by Scenedesmus sp. KMITL-OVG1.