Papan, Phakorn
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Item type:Publication, Foodomics of Rice Grains in Astrobiology: Spaceflight-Induced Modulation of Aroma, Texture, and Protein Digestibility in Thai Landrace Rice (466HM) Aboard the Shijian-19 (SJ-19) Low Earth Orbit Mission(2026-02-01) ;Tulyananda, Tatpong ;Yingchutrakul, Yodying ;Tantraphongsathon, Kakanang ;Khamsuwan, AtiggamasMoung-Ngam, PeeraponEnsuring a sustainable source of nutritious food is critical for long-duration space missions. Thai landrace rice 466HM exhibits high nutritional value and stress resilience, making it a promising candidate for space cultivation, yet its response to low Earth orbit (LEO) conditions remains poorly understood. This study compared rice grains maintained under terrestrial conditions with grains stored aboard the Shijian-19 (SJ-19) reusable satellite, orbiting at ~336 km for 13.5 days under microgravity (2<sup>−7</sup> × 10<sup>−7</sup> g) and an absorbed radiation dose of ~0.153 rad (Si). Volatile compound profiling, texture analysis of cooked grains, and simulated gastrointestinal digestion followed by peptide mass fingerprinting were performed. LEO-exposed rice grains exhibited a 1.67-fold increase in adhesiveness compared to Earth-based rice (p < 0.01), while hardness remained unchanged between the two groups (p > 0.05), alongside distinct alterations in flavor-related volatile compounds and peptide profiles. Principal component analysis revealed clear separation between Earth and LEO-exposed samples, indicating microgravity-associated shifts in digestible peptide composition. Cytotoxicity assessment using MTT assays in HT-29 and HepG2 cells confirmed the safety of both rice types. These findings demonstrate that orbital conditions influence the compositional, functional, and sensory attributes of rice, providing insights relevant to space agriculture and astronaut nutrition. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Integrative leaf–root metabolomics and root proteomics reveal proline-associated mechanisms underlying drought tolerance in RRIT 251 versus RRIM 600 rubber trees(2026-05-01) ;Tantraphongsathon, Kakanang ;Thanasuttanithi, Dechathon ;Khamsuwan, Atiggamas ;Sooksaksun, KrittadheeTitioatchasai, JatdilokDrought stress represents an increasing constraint on rubber tree (Hevea brasiliensis) cultivation under climate change. This study investigated drought responses in a grafted system, where contrasting scion genotypes (RRIM 600 and RRIT 251) systemically influence a shared, seedling-derived rootstock. An integrative framework combining metabolomics, root proteomics, and physiological measurements was applied under progressive soil moisture deficits (85%, 50%, and 30% field capacity). Multivariate analyses of metabolomic data revealed distinct clustering trends associated with drought severity and scion genotype, with more consistent responses observed in RRIT 251, particularly in the roots. Metabolic reprogramming was more pronounced in RRIT 251 roots, highlighted by the strong accumulation of proline, indicating osmoprotective responses. Root proteomics indicated differential stress-associated protein responses between scion types. RRIT 251 showed increased abundance of proteins related to redox regulation and membrane stability, including glutathione S-transferase (7.85-fold) and annexin (4.17-fold), whereas RRIM 600 exhibited a comparatively limited response. Physiological measurements supported these molecular findings, as RRIT 251 maintained higher Photosystem II efficiency and delayed leaf senescence under severe drought. Although based on a limited number of biological replicates, this integrative analysis suggests that RRIT 251 may promote a more coordinated root-associated drought response through scion-mediated systemic regulation. These findings highlight candidate molecular features for future validation and development of drought-resilient rubber tree cultivars. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Sensitive electrochemical detection of glycated hemoglobin (HbA1c) using cobalt metal-organic framework/two-dimensional molybdenum diselenide nanocomposite-based immunosensors amplified by polyoxometalate/DNA aptamer(2025-04-01) ;Anuthum, Siriporn; ;Pasena, Arnat ;Yimklan, SaranphongAramrat, ChanchanokClinical diagnosis and long-term diabetes management are advanced by monitoring glycated hemoglobin A1c (HbA1c) levels. New sensitive sandwich-like immunosensors for the diagnosis of early diabetes toward detecting HbA1c and hemoglobin (Hb) are demonstrated for the first time. DNA aptamers are used for signal amplification in the sensors for the detection of HbA1c and Hb. The immunosensors are constructed by coating with a cobalt-based metal-organic framework (Co-MOF)/two-dimensional molybdenum diselenide (2D MoSe<inf>2</inf>) composite onto a working electrode of an ItalSens screen-printed electrode (SPE) inserted into a Sensit/Smart Potentiostat affixed to a smartphone. After the immobilization of the antibodies, the detection is obtained by incubating the resultant SPEs in target solutions and then detecting the response of Keggin-type polyoxometalate (POM) bound on the DNA aptamer chains. In the selected potential window, the POM (silicotungstic acid, H<inf>4</inf>[α−SiW<inf>12</inf>O<inf>40</inf>]) used in this study exhibits the electron-transfer processes I and II ([α-SiW<inf>12</inf>O<inf>40</inf>]<sup>4−/5−</sup> and [α-SiW<inf>12</inf>O<inf>40</inf>]<sup>5−/6−</sup>, respectively) in the acidic buffer electrolyte. Our proposed device demonstrates exceptional performance in the recovery test of %HbA1c in healthy human plasma samples. The sensitivity, selectivity, and stability of this immunosensor are exceedingly outstanding, which makes it one of the potential analytical devices for diagnosing early diabetes by a %HbA1c assay.
