Pornpukdeewattana, Soisuda

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Preferred name
Pornpukdeewattana, Soisuda
Alternative Name
Pornpukdeewattana, S.
Main Affiliation
Email
soisuda.po@kmitl.ac.th

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    Item type:Publication,
    Thermal degradation kinetics and purification of C-phycocyanin from thermophilic and mesophilic cyanobacteria
    (2025-02-01)
    Chittapun, Supenya
    ;
    Suwanmanee, Kattiya
    ;
    Kongsinkaew, Chatchol
    ;
    Pornpukdeewattana, Soisuda
    ;
    Chisti, Yusuf
    The natural blue colorant C-phycocyanin (C-PC) has many potential applications but its poor heat stability limits its commercial use. This study compares the production and thermal stability of C-PC from two cyanobacteria: the thermophilic Thermosynechococcus sp. TUBT-T01 and the mesophilic Synechococcus cedrorum TISTR8589. Thermosynechococcus sp. produced nearly 1.9-fold more C-PC than S. cedrorum. Batch adsorption using a chromatographic cationic ion exchange resin (Streamline Direct HST1) was used to effectively purify the C-PC. The equilibrium adsorption capacity (Q<inf>eq</inf>) of the resin for C-PC was the highest at pH 5. At this pH, the Q<inf>eq</inf> for the thermophilic C-PC was 5.5 ± 0.1 mg mL⁻¹, whereas for the mesophilic C-PC it was 1.5 ± 0.2 mg mL⁻¹. Purification increased the concentration of the thermophilic C-PC by 5.9-fold, and that of mesophilic C-PC by 4.2-fold. The purity ratios of the final products from the two cyanobacteria were similar at ∼2.2. At 60 °C and pH 7, the C-PC of Thermosynechococcus sp. had ∼12-times longer half-life than the mesophilic C-PC; however, the productivity of the thermophilic C-PC was comparatively low because of a low biomass productivity of Thermosynechococcus sp.
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    Fed-batch strategies for growing β-carotene enriched yeast Rhodotorula paludigena using untreated molasses
    (2025-11-01)
    Kongsinkaew, Chatchol
    ;
    Chisti, Yusuf
    ;
    Ketudat-Cairns, Mariena
    ;
    Chittapun, Supenya
    ;
    Pornpukdeewattana, Soisuda
    The β-carotene-rich yeast Rhodotorula paludigena CM33 was grown in a 22-L bioreactor using sugarcane molasses in combination with several distinct fed-batch strategies: DO (dissolved oxygen)-stat; stepwise feeding; and hybrid feeding (an initial exponential feeding phase followed by a DO-stat operation). The impacts of feeding strategies on biomass production, carotenoid production and nutrient conversion efficiencies were evaluated. The stepwise feeding proved most effective, achieving: a final dry biomass concentration of 73.1 ± 2.7 g L<sup>−1</sup>; a β-carotene titer of 181.9 ± 1.0 mg L<sup>−1</sup>; a biomass volumetric productivity of 1.33 ± 0.05 g L<sup>−1</sup> h<sup>−1</sup>; and β-carotene productivity of 3.31 ± 0.02 mg L<sup>−1</sup> h<sup>−1</sup>. However, with this feeding strategy the sugar and nitrogen conversion efficiencies were the lowest: the β-carotene yield on total sugar was 0.92 ± 0.00 mg g<sup>−1</sup> and its yield on nitrogen was 5.47 ± 0.06 mg g<sup>−1</sup>. In contrast, the DO-stat operation was the most resource efficient, providing a biomass yield on sugar of 0.47 ± 0.02 g g<sup>−1</sup>, a biomass yield on nitrogen of 2.64 ± 0.05 g g<sup>−1</sup>, a β-carotene yield on sugar of 1.08 ± 0.04 mg g<sup>−1</sup>, and β-carotene yield on nitrogen of 6.08 ± 0.08 mg g<sup>−1</sup>. The hybrid strategy resulted in intermediate values of the various performance parameters. Raw sugarcane molasses proved to be excellent for high-cell-density culture of R. paludigena. A compromise between productivity and resource use efficiency may be required for commercial production of β-carotene especially if the substrate is expensive compared to the product.