Sanguanchaipaiwong, Vorapat

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Preferred name
Sanguanchaipaiwong, Vorapat
Alternative Name
Sanguanchaipaiwong, V.
Main Affiliation
Email
vorapat.sa@kmitl.ac.th

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    Enzymatic hydrolysis of cassava stems for butanol production of isolated Clostridium sp.
    (2020-02-01)
    Saekhow, B.
    ;
    Chookamlang, S.
    ;
    Na-u-dom, A.
    ;
    Leksawasdi, N.
    ;
    Sanguanchaipaiwong, V.
    This research focused on the hydrolysis of cassava stems (CS) and subsequent utilization as a carbon source for the cultivation of isolated Clostridium sp. To yield the highest amount of reducing sugars (RS), the studies on the pretreatment with sodium hydroxide (NaOH) and the hydrolysis with cellulases, amylases, and mixed enzymes were carried out. Afterwards, the hydrolysate was utilized for the cultivation of isolated Clostridium sp. Experimental results revealed that CS after 1.0 M NaOH pretreatment at 121 °C for 15 min and cellulase hydrolysis (Accellerase® 1500, 2500 CMC U/g CS) obtained 10.94 ± 0.29 g/L RS concentration. Hydrolysis of CS with amylases (Termamyl® 120, 1.2 U/g CS and AMG 300L™ 3.5 U/g CS) provided 34.85 ± 0.75 g/L RS and the maximum RS amount of 47.90 ± 0.39 g/L was obtained from the hydrolysis with mixed enzymes (Termamyl® 120, 1.2 U/g CS, AMG 300L™ 3.5 U/g CS followed by Accellerase® 1500, 2500 CMC U/g CS). From the cultivation of Clostridium sp. G10 using CS hydrolysate, the highest dry cell weight concentration of 1.28 ± 0.07 g/L was obtained with 11.68 ± 0.31 g/L butanol. It could be concluded that CS hydrolysate was comparable with glucose for utilization as a carbon source for butanol production.
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    PRETREATMENT AND HYDROLYSIS OF EUCALYPTUS FOR BIOBUTANOL PRODUCTION USING CLOSTRIDIUM SP. G10
    (2025-01-01)
    Rinthanapipat, Tunlaya
    ;
    Wongyou, Tarinee
    ;
    Sanguanchaipaiwong, Vorapat
    The primary objective of this study was to optimize the pretreatment and hydrolysis of eucalyptus for the cultivation of Clostridium sp. G10, a laboratory-isolated strain. Optimal alkali pretreatment conditions were determined to be 0.6 M NaOH at 121°C and 15 psi for 20 minutes, yielding a reducing sugar concentration of 11.91 g/L. Subsequent cellulose hydrolysis using ACCELERASE® 1500 (2000 endoglucanase U/g eucalyptus) for 24 hours further increased the reducing sugar concentration to 15.53 g/L. Eucalyptus hydrolysate was then utilized as a carbon source for Clostridium sp. G10 cultivation. In T6 medium supplemented with 50 g/L glucose, the maximum concentration of acetone-butanol-ethanol was 2.7 g/L. When T6 medium was supplemented with eucalyptus hydrolysate (50 g/L reducing sugar equivalent), the solvent concentration was found at 1.21 g/L. The results clearly indicate that the type of medium significantly impacts the production of acetone, butanol, and ethanol by Clostridium sp. G10. While eucalyptus hydrolysate can serve as a suitable carbon source, further optimization of hydrolysis conditions is necessary to achieve higher reducing sugar concentrations. Additionally, the removal of fermentation inhibitors from the hydrolysate is crucial for enhancing the efficiency of acetone-butanol-ethanol fermentation.
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    Pretreated and enzyme-hydrolyzed peanut shell for cultivation of clostridium sp. g10
    (2021-01-01)
    Sanguanchaipaiwong, V.
    ;
    Yoddamnern, P.
    ;
    Matngammnuang, W.
    ;
    Leksawasdi, N.
    Peanut shell (PS) is an agricultural residue and generated in large quantities at the peanut processing location. Normally, these lignocellulosic materials are utilized as feed or disposed of to landfill or burnt. In this research, PS was pretreated, enzymatically hydrolyzed, and utilized as a carbon source for Clostridium sp. G10 to produce butanol through acetone-butanol-ethanol (ABE) fermentation. PS was pretreated with H<inf>2</inf>SO<inf>4</inf> and NaOH (0- 1.0M). The highest reducing sugar concentration in the supernatant obtained from 0.4M H2SO4 was 23.1 g/L. Subsequently, the solid portion of pretreated PS was hydrolyzed at 50 °C for 24 h using commercial cellulose (ACCELLERASE™ 1500) with various treatment times duration and enzyme activity to solid ratios. The result suggested that enzyme activityof pretreated PS weight ratio of 1250 carboxymethyl cellulose (CMC) U:1 g yielded the maximum concentration of reducing sugars(12.74 g/L). Mixture of acidic pretreatment hydrolysate and enzyme hydrolysate contained41.2 g/L glucose concentration. Clostridium sp. G10 was then cultured in three conditions; T6 medium with 45 g/L glucose as a control, T6 medium prepared with 45 g/L reducing sugar equivalent of PS hydrolysate, and PS hydrolysate with 45 g/L reducing sugar equivalent at 37°C for 168 h. The implementation ofPS hydrolysate solely as a carbon source produced the butanol concentration of 10.8 g/L. This was not significantly different (p < 0.05) from using T6 medium with glucose (9.3 g/L). The highest butanol production of 15.2 g/Lwas yielded from T6 medium with PS hydrolysate. It could be concluded that hydrolyzed PS could be utilized as a carbon source for production of butanol and ethanol.
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    DILUTE ACID HYDROLYZED SOYBEAN MEAL FOR CULTIVATION OF ISOLATED CLOSTRIDIUM SP. G10
    (2022-01-01)
    Sanguanchaipaiwong, Vorapat
    ;
    Sabua, Chonthicha
    ;
    Hemnusoornnanon, Nutcha
    ;
    Nuangpanom, Phornnapa
    ;
    Leksawasdi, Noppol
    Soybean meal is a by-product from soybean oil manufacturing. It contained, on the mass basis, crude fiber (6.3%) and protein (47.6%) after oil extraction. The aim of study was to investigate the cultivation of isolated Clostridium sp. G10 using soybean meal hydrolysate as a N-and C-source in fermentation medium. Soybean meal hydrolyzed with 0.02 to 0.1 M of HCl and H2SO4 and was heated at 121 °C, 15 psi for 10-30 min. The result showed that hydrolyzing soybean with 0.1 M HCl and 20-min heating period were the optimal conditions to obtain soluble protein (24.45 ±2.21 g/L) and reducing sugar (13.12 ±1.09 g/L). To study the effect of N-and C-sources replacement in T6 medium, soybean meal hydrolysate was utilized to replace yeast extract and tryptone resulting in 8 g/L soluble protein and used as a C-source with the addition of glucose with 50 g/L reducing sugar equivalent. The butanol concentration (10.80 ±0.44 g/L) was slightly lowered than that of control T6 medium (12.15 ±0.07 g/L). These results demonstrated that Clostridium sp. was able to uptake the N-and C-sources contained in the hydrolyzed soybean meal. However, a C-source alternative is required to mitigate the cost of raw materials.