Sanguanchaipaiwong, Vorapat

Preferred name

Alternative Name

Sanguanchaipaiwong, V.

Main Affiliation

School of Science

vorapat.sa@kmitl.ac.th

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Using Glycerol as a Sole Carbon Source for Clostridium beijerinckii Fermentation
(2017-01-01)
Sanguanchaipaiwong, Vorapat
;
Leksawasdi, Noppol
Butanol is a promising biofuel with its fuel properties which are similar to gasoline and able to be synthesized from acetone-butanol-ethanol fermentation of Clostridium sp. This study has been focused on using glycerol, a by-product from biodiesel manufacturing as a carbon source for Clostridium beijerinckii TISTR 1390. The culture was cultivated at 37°C in P2 medium with 20-60 g/L glycerol under anaerobic condition, compared with glucose as a control. Samples were collected periodically for the analysis of viable cell concentration, reducing sugar and metabolite concentrations. It has been found that C. beijerinckii grown much slower in glycerol. After 168 h cultivation, the cell growth in 20 g/L glycerol has achieved the maximum concentration of 4.78 × 106 CFU/mL. In P2 medium containing glucose, the amount of viable cell (3.93 x 106 CFU/mL with 20 g/L glucose) had reached the maximum level at 24 h. The much longer period for highest cell number might be the reason that C. beijerinckii could not produce butanol from merely glycerol. On the other hand, the butanol concentration of 8.12 g/L was obtained from 60 g/L glucose. Glycerol probably induced C. beijerinckii in switching to a reductive pathway.
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Butanol production by Clostridium beijerinckii from pineapple waste juice
(2018-01-01)
Sanguanchaipaiwong, Vorapat
;
Leksawasdi, Noppol
Renewable energy has received increasing attention, due to global energy crisis and limited supply of fossil fuels. Butanol is one of the alternative biofuels with the similar energy properties as gasoline, such as, energy density and heat of vaporization. It could be produced by Clostridia via acetone-butanol-ethanol fermentation from various renewable sources. To obtain economical raw materials, pineapple waste juice was utilized as a carbon source for Clostridium beijerinckii TISTR 1461. The juice was collected from ‘Pattavia' pineapple waste and utilized to prepare culture medium. The maximum viable C. beijerinckii concentration (2.40 ± 0.12 x 108 CFU/mL) was obtained at 168 h of cultivation with pineapple waste juice under anaerobic condition at 37 °C. The butanol concentration of 3.14 ± 0.16 g/L was subsequently produced. A yield of 0.08 g butanol∙g-1 reducing sugars was achieved suggesting the necessity to improve fermentation process for higher level of butanol concentration.
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Effect of Media on Acetone-Butanol-Ethanol Fermentation by Isolated Clostridium spp.
(2017-01-01)
Buakhiaw, Budsaba
;
Sanguanchaipaiwong, Vorapat
Butanol is one of the important industrial chemicals and currently recognized as an alternative and renewable biofuel. It has been receiving an increased attention due to the energy crisis and the decrease of petroleum. Butanol can be obtained from several anaerobic microorganisms, which belong to genus Clostridium. The problem is the ability of Clostridium sp. to produce low concentration of butanol. Consequently, the screening and isolating for new Clostridium strains which can produce high amount of biobutanol has been required. Clostridium sp. strain G10 was isolated from marsh sediment in King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand. Its capability of butanol production was 5.89 g/L with a total acetone-butanol-ethanol (ABE) concentration of 8.48 g/L under anaerobic conditions. The medium composition significantly affected the performance of (ABE) production by strain G10. To investigate the effect of media on solvent production by isolated strain, four media, including RCM, T6, GYCC, and P2 medium (30 g/L glucose in each medium) were utilized to cultivate Clostridium sp. strain G10. The results revealed that, T6 medium could produce the highest biobutanol concentration (13.49 g/L) and ABE was 16.22 g/L (acetone 2.63 g/L and ethanol 0.10 g/L).
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PRETREATMENT AND HYDROLYSIS OF EUCALYPTUS FOR BIOBUTANOL PRODUCTION USING CLOSTRIDIUM SP. G10
(2025-01-01)
Rinthanapipat, Tunlaya
;
Wongyou, Tarinee
;
Sanguanchaipaiwong, Vorapat
The primary objective of this study was to optimize the pretreatment and hydrolysis of eucalyptus for the cultivation of Clostridium sp. G10, a laboratory-isolated strain. Optimal alkali pretreatment conditions were determined to be 0.6 M NaOH at 121°C and 15 psi for 20 minutes, yielding a reducing sugar concentration of 11.91 g/L. Subsequent cellulose hydrolysis using ACCELERASE® 1500 (2000 endoglucanase U/g eucalyptus) for 24 hours further increased the reducing sugar concentration to 15.53 g/L. Eucalyptus hydrolysate was then utilized as a carbon source for Clostridium sp. G10 cultivation. In T6 medium supplemented with 50 g/L glucose, the maximum concentration of acetone-butanol-ethanol was 2.7 g/L. When T6 medium was supplemented with eucalyptus hydrolysate (50 g/L reducing sugar equivalent), the solvent concentration was found at 1.21 g/L. The results clearly indicate that the type of medium significantly impacts the production of acetone, butanol, and ethanol by Clostridium sp. G10. While eucalyptus hydrolysate can serve as a suitable carbon source, further optimization of hydrolysis conditions is necessary to achieve higher reducing sugar concentrations. Additionally, the removal of fermentation inhibitors from the hydrolysate is crucial for enhancing the efficiency of acetone-butanol-ethanol fermentation.
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Pretreated and enzyme-hydrolyzed peanut shell for cultivation of clostridium sp. g10
(2021-01-01)
Sanguanchaipaiwong, V.
;
Yoddamnern, P.
;
Matngammnuang, W.
;
Leksawasdi, N.
Peanut shell (PS) is an agricultural residue and generated in large quantities at the peanut processing location. Normally, these lignocellulosic materials are utilized as feed or disposed of to landfill or burnt. In this research, PS was pretreated, enzymatically hydrolyzed, and utilized as a carbon source for Clostridium sp. G10 to produce butanol through acetone-butanol-ethanol (ABE) fermentation. PS was pretreated with H<inf>2</inf>SO<inf>4</inf> and NaOH (0- 1.0M). The highest reducing sugar concentration in the supernatant obtained from 0.4M H2SO4 was 23.1 g/L. Subsequently, the solid portion of pretreated PS was hydrolyzed at 50 °C for 24 h using commercial cellulose (ACCELLERASE™ 1500) with various treatment times duration and enzyme activity to solid ratios. The result suggested that enzyme activityof pretreated PS weight ratio of 1250 carboxymethyl cellulose (CMC) U:1 g yielded the maximum concentration of reducing sugars(12.74 g/L). Mixture of acidic pretreatment hydrolysate and enzyme hydrolysate contained41.2 g/L glucose concentration. Clostridium sp. G10 was then cultured in three conditions; T6 medium with 45 g/L glucose as a control, T6 medium prepared with 45 g/L reducing sugar equivalent of PS hydrolysate, and PS hydrolysate with 45 g/L reducing sugar equivalent at 37°C for 168 h. The implementation ofPS hydrolysate solely as a carbon source produced the butanol concentration of 10.8 g/L. This was not significantly different (p < 0.05) from using T6 medium with glucose (9.3 g/L). The highest butanol production of 15.2 g/Lwas yielded from T6 medium with PS hydrolysate. It could be concluded that hydrolyzed PS could be utilized as a carbon source for production of butanol and ethanol.
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DILUTE ACID HYDROLYZED SOYBEAN MEAL FOR CULTIVATION OF ISOLATED CLOSTRIDIUM SP. G10
(2022-01-01)
Sanguanchaipaiwong, Vorapat
;
Sabua, Chonthicha
;
Hemnusoornnanon, Nutcha
;
Nuangpanom, Phornnapa
;
Leksawasdi, Noppol
Soybean meal is a by-product from soybean oil manufacturing. It contained, on the mass basis, crude fiber (6.3%) and protein (47.6%) after oil extraction. The aim of study was to investigate the cultivation of isolated Clostridium sp. G10 using soybean meal hydrolysate as a N-and C-source in fermentation medium. Soybean meal hydrolyzed with 0.02 to 0.1 M of HCl and H2SO4 and was heated at 121 °C, 15 psi for 10-30 min. The result showed that hydrolyzing soybean with 0.1 M HCl and 20-min heating period were the optimal conditions to obtain soluble protein (24.45 ±2.21 g/L) and reducing sugar (13.12 ±1.09 g/L). To study the effect of N-and C-sources replacement in T6 medium, soybean meal hydrolysate was utilized to replace yeast extract and tryptone resulting in 8 g/L soluble protein and used as a C-source with the addition of glucose with 50 g/L reducing sugar equivalent. The butanol concentration (10.80 ±0.44 g/L) was slightly lowered than that of control T6 medium (12.15 ±0.07 g/L). These results demonstrated that Clostridium sp. was able to uptake the N-and C-sources contained in the hydrolyzed soybean meal. However, a C-source alternative is required to mitigate the cost of raw materials.

Sanguanchaipaiwong, Vorapat

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