Sanguanchaipaiwong, Vorapat

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Preferred name
Sanguanchaipaiwong, Vorapat
Alternative Name
Sanguanchaipaiwong, V.
Main Affiliation
Email
vorapat.sa@kmitl.ac.th

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    Item type:Publication,
    PRETREATMENT AND HYDROLYSIS OF EUCALYPTUS FOR BIOBUTANOL PRODUCTION USING CLOSTRIDIUM SP. G10
    (2025-01-01)
    Rinthanapipat, Tunlaya
    ;
    Wongyou, Tarinee
    ;
    Sanguanchaipaiwong, Vorapat
    The primary objective of this study was to optimize the pretreatment and hydrolysis of eucalyptus for the cultivation of Clostridium sp. G10, a laboratory-isolated strain. Optimal alkali pretreatment conditions were determined to be 0.6 M NaOH at 121°C and 15 psi for 20 minutes, yielding a reducing sugar concentration of 11.91 g/L. Subsequent cellulose hydrolysis using ACCELERASE® 1500 (2000 endoglucanase U/g eucalyptus) for 24 hours further increased the reducing sugar concentration to 15.53 g/L. Eucalyptus hydrolysate was then utilized as a carbon source for Clostridium sp. G10 cultivation. In T6 medium supplemented with 50 g/L glucose, the maximum concentration of acetone-butanol-ethanol was 2.7 g/L. When T6 medium was supplemented with eucalyptus hydrolysate (50 g/L reducing sugar equivalent), the solvent concentration was found at 1.21 g/L. The results clearly indicate that the type of medium significantly impacts the production of acetone, butanol, and ethanol by Clostridium sp. G10. While eucalyptus hydrolysate can serve as a suitable carbon source, further optimization of hydrolysis conditions is necessary to achieve higher reducing sugar concentrations. Additionally, the removal of fermentation inhibitors from the hydrolysate is crucial for enhancing the efficiency of acetone-butanol-ethanol fermentation.
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    DILUTE ACID HYDROLYZED SOYBEAN MEAL FOR CULTIVATION OF ISOLATED CLOSTRIDIUM SP. G10
    (2022-01-01)
    Sanguanchaipaiwong, Vorapat
    ;
    Sabua, Chonthicha
    ;
    Hemnusoornnanon, Nutcha
    ;
    Nuangpanom, Phornnapa
    ;
    Leksawasdi, Noppol
    Soybean meal is a by-product from soybean oil manufacturing. It contained, on the mass basis, crude fiber (6.3%) and protein (47.6%) after oil extraction. The aim of study was to investigate the cultivation of isolated Clostridium sp. G10 using soybean meal hydrolysate as a N-and C-source in fermentation medium. Soybean meal hydrolyzed with 0.02 to 0.1 M of HCl and H2SO4 and was heated at 121 °C, 15 psi for 10-30 min. The result showed that hydrolyzing soybean with 0.1 M HCl and 20-min heating period were the optimal conditions to obtain soluble protein (24.45 ±2.21 g/L) and reducing sugar (13.12 ±1.09 g/L). To study the effect of N-and C-sources replacement in T6 medium, soybean meal hydrolysate was utilized to replace yeast extract and tryptone resulting in 8 g/L soluble protein and used as a C-source with the addition of glucose with 50 g/L reducing sugar equivalent. The butanol concentration (10.80 ±0.44 g/L) was slightly lowered than that of control T6 medium (12.15 ±0.07 g/L). These results demonstrated that Clostridium sp. was able to uptake the N-and C-sources contained in the hydrolyzed soybean meal. However, a C-source alternative is required to mitigate the cost of raw materials.