Sriket, Chodsana
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Preferred name
Sriket, Chodsana
Alternative Name
Sriket, C.
Main Affiliation
Email
chodsana.sr@kmitl.ac.th
4 results
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Item type:Publication, Impact of Bio-calcium from Nile Tilapia Bone as a Supporting Material on the Stability Enhancement of Immobilized Probiotics(2025-07-01) ;Petsong, Kantiya ;Yarnpakdee, Suthasinee ;Senphan, Theeraphol; Kingwascharapong, PassakornThis study presented the potential of bio-calcium powder (BC) from Nile tilapia bone on enhancement of probiotics (Lactobacillus acidophilus) stability as supporting material in probiotic immobilization and novel nutritive resource, particularly calcium. Combined immobilization techniques of absorption using BC at different concentrations (0, 10, and 15%) and encapsulation using fish collagen peptide (CP) (10%) were applied. Immobilized probiotics (IP) were investigated for resistance through various adverse conditions, including freeze-drying, severe pH and temperature levels, and gastro-intestinal tract (GI) conditions. After freeze-drying, all IPs showed resistance with > 90% survivability. Under various pHs, all samples revealed non-significant differences on cell survival under neutral and alkaline conditions throughout the tested incubation period (P > 0.05). Notably decreased survivability was detected in IP with BC compared to other samples, especially under acid condition. IP with 10 and 15% BC (IP-10 and IP-15) showed higher stability to thermal condition (55 °C) with increased incubation time, compared with those without BC (IP-0). After GI digestion, IP with BC reveals a better survivability with greater recovery ability, compared with IP-0 (P < 0.05). Calcium solubility increased with BC level in a dose-dependent manner (P < 0.05). Based on morphology and microscopy analysis, IP with BC illustrated cells attached to the rough surface of BC, which was coated with collagen peptide. Elemental mappings and distribution content suggested BC could be a great supporting material and calcium resource, particularly IP-15. Therefore, the absorption by BC together with encapsulation by collagen peptide could render a highly stable immobilized probiotic and a good calcium resource. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A comprehensive study of diverse techniques for enhanced physicochemical and structural properties of bio-calcium from hybrid catfish bone(2023-12-01); ;Niwet, Janejira ;Kuimalee, Surasak ;Benjakul, SoottawatYarnpakdee, SuthasineeThis research examined the impact of various comparative preparation methods on enhanced physicochemical, and structural properties of bio-calcium (BC) from hybrid catfish bone. Raw hybrid catfish bones (RB) were used to make bio-calcium in four steps. High-pressure water jet processing produced washed bone (WB), soaking the bone in an alkaline solution to remove proteins (PR), immersing it in ethanol to remove lipids (LR), then bleaching, and grinding it to make bio-calcium powder. The BC had greater lightness values (L*) (p < 0.05), and contained significant calcium, and phosphorus. The SDS-PAGE showed three significant protein bands with molecular weights of 250, 133, and 116 kDa. The crystalline structure of hydroxyapatite in BC was confirmed using X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The percentage of crystallinity of BC had a notable rise, increasing from the RB to BC of 36.25%–45.32%. The BC powder exhibited a consistent distribution of particle sizes, ranging from 2 to 10 μm. Additionally, the analysis of elemental profiles, and mappings using scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) revealed that BC exhibits a significant concentration of calcium, and phosphorus. BC showed superior %bioavailability of calcium (18.94%) in an in vitro simulated gastrointestinal system (GIMs) compared to RB (10.58%), and calcium carbonate (11.19%). Hence, the BC production process proved to be a viable method for acquiring premium-grade BC, which holds potential for further development as a calcium dietary supplement. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Low-sodium Chaya Leaf Seasoning Powder with Potassium Chloride Substitution: Nutritional, Antioxidant, and Microbial Quality Assessment(2025-01-01) ;Senphan, Theeraphol ;Puangtong, Kotchaporn ;Namdamrassiri, Benyapa; Hoque, Md SazedulThis study investigates the development of a low-sodium seasoning powder using Chaya (Cnidoscolus chayamansa), popularly known as Mexican spinach, by substituting sodium chloride (NaCl) with potassium chloride (KCl) at various ratios. The antioxidant capabilities of dried Chaya leaves, dried at 60°C for five hours, were tested using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) techniques, demonstrating excellent free radical scavenging activity and making it suitable for low sodium seasoning compositions. The substitution of 25% KCl was the most favored option, as it effectively balanced flavor and health advantages without causing noticeable changes in appearance, color, and aroma. This recipe successfully reduced the sodium content to 1171 mg/100 g while maintaining Chaya’s original nutritional values, which include 23.57 g/100 g of protein, 4.77 g/100 g of fats, and 54.12 g/100 g of carbohydrates, as well as dietary fibers. Additionally, the formulation demonstrated physicochemical stability, as indicated by an ash content of 14.00 g/100 g, moisture content of 3.51 g/100g, and a total energy of 353.69 kcal/100 g. The safety of this new seasoning is validated by its microbiological quality, which is indicated by a total bacterial count of 2.6×10<sup>4</sup> cfu/g and the absence of mold and yeast. This emphasizes the potential of the seasoning to promote healthy dietary choices by reducing sodium intake without affecting sensory appeal. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods(2024-11-01) ;Thirabunyanon, Mongkol ;Mungmueang, Natthapong ;Daengprok, Wichittra ;Ngampeerapong, ChitrapornKarnjanapratum, SupatraThe present research examines different techniques for isolating proteins from Cordyceps militaris fruiting body powder (COR), namely ammonium sulfate precipitation (ASP), organic solvent precipitation (OSP), and isoelectric precipitation (IEP). ASP yielded the highest protein content (78.90 %), effectively concentrating proteins, while OSP and IEP also produced substantial yields (57.41 % and 69.06 %, respectively). Color analysis revealed differences among isolates, with ASP resulting in a darker hue. ASP and IEP showed higher red and yellow components, respectively. Essential amino acid content was significantly higher in ASP isolate compared to COR, indicating effective amino acid concentration. Thermal analysis revealed different decomposition temperatures and enthalpy values among isolates. OSP displayed the highest phenolic content, while IEP exhibited the highest flavonoid content. Antioxidant assays showed ASP to have the highest DPPH-RSA and ABTS-RSA, while IEP showed the highest FRAP value. ASP demonstrated anticancer activity against HT-29 colon cancer cells inducing cell death apoptosis and inhibiting cell migration, with an IC<inf>50</inf> of 360.63 µg/mL, comparable to Paclitaxel. These findings suggest that Cordyceps militaris protein isolate, particularly from ASP, has significant antioxidant and anticancer potential, supporting its potential as a natural alternative to synthetic drugs with fewer side effects.
