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    The effectiveness of star anise nanoemulsion and chemical insecticide for controlling of beet armyworm
    (2024-05-01) ;
    Pumnuan, J.
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    Thongsaiklaing, T.
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    Thipmanee, K.
    The effectiveness of star anise (Illicium verum) nanoemulsion and deltamethrin (chemical insecticide) was tested with beet armyworm (Spodoptera exigua) to evaluate their mortality, antifeedant, and growth inhibition effects at proper concentrations. As a result, at the obtained highest effectiveness, star anise nanoemulsion required 0.35% concentration for the maximum effectiveness with 100% mortality, antifeedant and growth inhibition effects against beet armyworm, when compared with the control (Tween20 and water), while deltamethrin exhibited a lower rate. Therefore, star anise nanoemulsion seem to be utilized as a highly effective and environmentally friendly insecticide to control beet armyworm.
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    Identification of new position of DNA insertion and 24 bp INDEL mutation polymorphism in Prolactin gene promoter of Thai native chickens
    (2025-07-01)
    Thongsaiklaing, T.
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    Datumada, H.
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    Morris, J.
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    This research identified a 24-bp INDEL (-358) on the promoter region of the PRL gene in Thai native chickens. The allele frequencies for allele I and allele D were 0.20 and 0.80, respectively. The genotype frequencies were II (0.028), ID (0.349), and DD (0.622), respectively. The heterozygosity values comprised the Ho (0.350), higher than the He value (0.323). Moreover, our findings revealed new DNA duplication on the PRL promoter region, with three distinct polymorphisms of 30 bp (-358), 24 bp (-358), and 14 bp (-358), respectively.
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    Adulticidal activity of star anise, turmeric, cloves and combinations against houseflies
    (2025-01-01)
    Sinthusiri, J.
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    Puwanard, C.
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    Thongsaiklaing, T.
    The small droplet size could increase the absorbed ingredients and high stability. The star anise nanoemulsion was determined optimum efficacy, with a 1% concentration resulting in the highest knockdown and mortality rate of 100%. Its KT<inf>50</inf> and mortality effective values were 28 min and 36 times, respectively. Furthermore, the adult stage of houseflies is found to be susceptible to star anise nanoemulsion. In contrast, cypermethrin was less toxic to it, with a high KT<inf>50</inf> and resistance to adult houseflies. Consequently, the star anise nanoemulsion could be developed into an efficient and safe environment for controlling housefly populations.
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    Antifungal efficacy of stingless bee honey extracts against a black mould causing pathogen Lasiodiplodia spp
    (2026-03-01)
    Datumada, H.
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    Niyomdecha, A.
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    Ramdoung, T.
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    Sudwisai, W.
    Fungal species causing the black sooty mould were isolated from fruit peels and subjected to molecular genetic analysis. The nucleotide sequences were found to be 100% identical to the fungus Lasiodiplodia iranensis. Results from testing the antifungal properties of stingless bee honey extracts showed that the extract from the H. itama species exhibited the highest inhibitory effect, followed by T. pagdeni, G. thoracica, and T. fuscobalteata. The average inhibition values were 21.43+1.27, 18.54+1.50, 17.51+1.76, and 17.18+0.91 mm., respectively.
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    Item type:Publication,
    Plant essential oils, trans-anethole and eugenol, for housefly knockdown and mortality
    (2025-03-01)
    Sittichok, S.
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    Sinthusiri, J.
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    Thongsaiklaing, T.
    The knockdown and mortality of some compounds from natural oils (trans-anethole, eugenol and a combination of trans-anethole and eugenol) against adult houseflies (Musca domestica) compared with cypermethrin was investigated. The knockdown and mortalities were highest at 100% of 1% trans-anethole with KT50= 31 min and LT50 = 31 min. The mortality index of trans-anethole was 82.1 times that of cypermethrin. In contrast, 1% eugenol had knockdown and mortality was 36% and 0.5% trans-anethole + 0.5% eugenol had knockdown and mortality of 68%, with mortality indexes of 1.6 and 3.2 times versus cypermethrin. The trans-anethole high toxicity and low residue made it a superior control for houseflies.
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    Item type:Publication,
    Insecticidal efficiency of plant essential oil nanoemulsion formulas against Spodoptera exigua
    (2024-01-01) ;
    Pumnuan, J.
    ;
    Thongsaiklaing, T.
    ;
    Thipmanee, K.
    The findings revealed that the nEO formula of I. verum and C. longa demonstrated optimal effectiveness, with a concentration of 0.35% resulting in the highest mortality rate of 43.3%, 100% antifeedant effect and 85.0% growth inhibition activities in pupae. Additionally, a concentration of 0.25% led to 100% growth inhibition activities in adults. Consequently, the nEO formulas of I. verum and C. longa promise for the future development of botanical insecticides targeting the control of S. exigua.