Passara, Hataichanok

Preferred name

Alternative Name

Passara, H.

Main Affiliation

School of Agricultural Technology

hataichanok.pa@kmitl.ac.th

Now showing 1 - 10 of 16

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Synergistic Larvicidal and Pupicidal Effects of Monoterpene Mixtures Against Aedes aegypti with Low Toxicity to Guppies and Honeybees
(2025-07-01)
Sittichok, Sirawut
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Passara, Hataichanok
;
Moungthipmalai, Tanapoom
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Sinthusiri, Jirisuda
;
Murata, Kouhei
The present study evaluated the larvicidal and pupicidal activities of pure and mixed monoterpene formulations—eucalyptol, geranial, trans-anethole, and trans-cinnamaldehyde—against Aedes aegypti and compared them with 1% (w/w) temephos. Safety bioassays of all formulations on non-target species confirmed their safety. The combined mixture of eucalyptol + trans-anethole at 400 µg/mL exhibited stronger larvicidal activity, with an LC<inf>50</inf> of 176 µg/mL, while the combination of trans-anethole + geranial at 400 µg/mL exhibited stronger pupicidal activity with an LC<inf>50</inf> of 167 µg/mL. Both formulations were more effective than a 1% temephos. All the mixture formulations were more strongly synergistic compared to pure formulations, with an increased mortality value (IMV) of 25% to 95%. External morphological aberrations observed at death included swelling of the respiratory system. Importantly, all the formulations were safe for two non-target species: guppies (Poecilia reticulata) and honeybees (Apis mellifera). The combination formulations are strong larvicides and pupicides for controlling Ae. Aegypti, which will help reduce the spread of viruses carried by this vector.
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Impact of botanical essential oils on pupation and survival of Musca domestica L.
(2026-05-01)
Moungthipmalai, T.
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Sriboonjit, N.
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Khuendee, P.
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Phunglakchai, J.
;
Thongsaiklaing, M.
Among the tested botanical extracts, Illicium verum (star anise) essential oil at 10% demonstrated the highest efficacy in disrupting normal pupal development. Only 65.00% of individuals successfully emerged as fully developed adults after a 10-day exposure period. A notable proportion exhibited transitional deformities, particularly malformed pupal-adult intermediates, indicating interference with metamorphic progression. In comparison, cypermethrin, a commonly applied synthetic insecticide at the same concentration, resulted in a lower mortality rate of 39.67% under identical experimental conditions. The superior performance of star anise oil highlighted its potential as a plant-derived alternative for insect control. These findings suggested that the incorporation of such natural products into integrated pest management strategies may reduce dependence on conventional synthetic chemicals.
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Head lice prevalence in suburban Bangkok, Thailand, after the COVID-19 pandemic and herbal shampoo treatment
(2026-01-01)
Sinthusiri, J.
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Sittichok, S.
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Passara, H.
;
Soonwera, M.
The prevalence of pediculosis among 3,461 girls from 10 government kindergartens and primary schools in Ladkrabang district was 48.1% in the 1st semester (May-September 2022) and 44.6% in the 2nd semester (November 2022-March 2023), higher among primary school girls, at 51.8-55.4%, than in kindergarteners, at 30.2-33.1%. Over 80% of the infested girls came from a low-income family (<3,000 USD per year). The Zingiberaceae essential oil and shampoo treatments were highly effective with 100% cure rate for every infested girl after the 2nd treatment (on Day 10, after the 1st treatment on Day 1). Effective strategies for reducing head lice prevalence are improved living standards and head lice eradication by treatments with effective natural pediculicides and improved head lice educational program.
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Binary Mixtures of Essential Oils: Potent Housefly Adulticides That Are Safe Against Non-Target Species
(2025-08-01)
Passara, Hataichanok
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Sittichok, Sirawut
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Moungthipmalai, Tanapoom
;
Laosinwattana, Chamroon
;
Murata, Kouhei
In this study, we investigated the insecticidal potential of Eucalyptus globulus Labill. and Cymbopogon citratus Stapf essential oils (EOs), both alone and in synergistic blends with their primary active compounds, against adult houseflies (Musca domestica L.). Toxicity assessments were also conducted on non-target organisms—dwarf honeybees (Apis florea Fabricius) and guppies (Poecilia reticulata Peters)—to evaluate environmental safety. All binary EO mixtures demonstrated superior efficacy compared to individual EOs and the synthetic pyrethroid α-cypermethrin (1% positive control). The most potent formulation, combining 2.5% (v/v) geranial with 2.5% (v/v) E. globulus EO, exhibited a synergistic effect, achieving complete fly mortality (LT<inf>50</inf>: 0.06 h). This mixture’s mortality index significantly exceeded those of single-component formulations, with a mortality index of 0.22, confirming greater toxicity to flies than α-cypermethrin. Importantly, all the tested EOs and their blends were non-toxic to honeybees and guppies; in comparison, α-cypermethrin caused significant harm. These findings highlight the 2.5% (v/v) geranial + 2.5% (v/v) E. globulus EO blend as a highly effective and environmentally friendly alternative to conventional insecticides. Further research is recommended to optimize its formulation for practical use in sustainable fly management.
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Impact of Nigrospora oryzae-Derived Natural Products on Photosynthesis and Oxidative Stress in Eichhornia crassipes
(2025-01-01)
Laosinwattana, Chamroon
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Manichart, Nutcha
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Wichittrakarn, Pattharin
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Yoneyama, Kaori
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Teerarak, Montinee
Interest in natural herbicides has been growing due to government policies restricting synthetic herbicide use in many countries. In that regard, this study investigates the potential of Nigrospora oryzae extract as a natural herbicide against the aquatic invasive weed Eichhornia crassipes. A stable formulation was developed with a droplet size of 36.44 ± 0.36 nm and a zeta potential of -62.59 mV. Pot-based experiments revealed the N. oryzae extract induced 38.33% phytotoxicity within 24 hours, increasing to 84.72% by 28 days post-treatment. Scanning electron microscopy demonstrated morphoanatomical changes in epidermal tissue and stroma of E. crassipes, such as erosion of epicuticular waxes and degeneration of epidermis cells. The treatment significantly reduced the photosynthetic pigment content while increasing hydrogen peroxide (46.26%), malondialdehyde (17.49%), and proline (19.16%) levels, causing cellular electrolyte leakage. Activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase were significantly elevated (p<0.05), indicating oxidative damage. These findings demonstrate that N. oryzae extract can disrupt growth and photosynthesis and induce oxidative stress in E. crassipes, suggesting its potential as a source of natural herbicide for industrial application.
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Development of a Melia azedarach L. based natural herbicide: formulation and efficacy studies
(2025-08-01)
Wichittrakarn, Pattharin
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Manichart, Nutcha
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Laosinwattana, Chamroon
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Charoenying, Patchanee
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Sikhao, Potjana
This study evaluated the phytotoxic activity of Melia azedarach L. leaf extract against Echinochloa crus-galli and Phaseolus lathyroides. Crude extracts were obtained using sequential solvent separation, of which the dichloromethane fraction exhibited the highest phytotoxicity for both weed species. However, P. lathyroides appeared more sensitive to the extracts compared to E. crus-galli. GC-MS analysis of the dichloromethane crude extract identified phytol (29.14 %), linolenic acid (21.74 %), and palmitic acid (7.20 %) as the main chemical components. Three formulations, a soluble liquid (SL), pellet, and wettable powder, were developed and evaluated for their effects on seed germination and seedling growth. The SL formulation showed the highest efficacy in inhibiting germination (p < 0.05). Further testing of its pre-emergence utility via soil application in greenhouse conditions confirmed the herbicidal efficacy, effectively suppressing P. lathyroides emergence (36.8 % over control) and reducing growth parameters. Mechanistic assays showed the SL product to affect seed imbibition, α-amylase activity, and cell division in a dose-dependent manner, suggesting these as potential mechanisms of action. This study highlights the potential of a M. azedarach-based SL product as an alternative to synthetic herbicides, potentially leading to the development of a new botanical herbicide.
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Identification of new position of DNA insertion and 24 bp INDEL mutation polymorphism in Prolactin gene promoter of Thai native chickens
(2025-07-01)
Thongsaiklaing, T.
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Datumada, H.
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Morris, J.
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Passara, H.
This research identified a 24-bp INDEL (-358) on the promoter region of the PRL gene in Thai native chickens. The allele frequencies for allele I and allele D were 0.20 and 0.80, respectively. The genotype frequencies were II (0.028), ID (0.349), and DD (0.622), respectively. The heterozygosity values comprised the Ho (0.350), higher than the He value (0.323). Moreover, our findings revealed new DNA duplication on the PRL promoter region, with three distinct polymorphisms of 30 bp (-358), 24 bp (-358), and 14 bp (-358), respectively.
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Synergistic Adulticidal Activity of Lemongrass (Cymbopogon citratus), Star Anise (Illicium verum), Nutmeg (Myristica fragrans) Essential Oil and Their Components Against the Housefly (Musca domestica) and Their Safety for Key Non-Target Organisms
(2026-04-01)
Passara, Hataichanok
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Laosinwattana, Chamroon
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Moungthipmalai, Tanapoom
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Murata, Kouhei
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Soonwera, Mayura
Botanical insecticides containing a mixture of plant essential oils (EOs) are considered suitable for the management of houseflies (M. domestica). The adulticidal efficacies of single EOs and mixtures of EOs, including lemongrass (C. citratus), star anise (I. verum), nutmeg (M. fragrans), and their components (geranial, trans-anethole, and α-pinene), against houseflies were determined in comparison to 2% (w/v) α-cypermethrin as the positive control and distilled water as the negative control. The mixture of star anise EO (1%) + geranial (1%) was the most effective adulticide, superseding single EOs, other combinations of EOs, and its active component, α-cypermethrin, and distilled water. This mixture was highly synergistic and was found to be over 74% more toxic than all single EOs and almost 2.6 times more toxic than α-cypermethrin. Furthermore, the tested EOs did not cause mortality in guppies (P. reticulata) or earthworms (E. fetida), and caused a maximum of 48% mortality in honeybees (A. mellifera) at 24 h; by contrast, α-cypermethrin led to 100% mortality in honeybees within 0.5 h and in guppies and earthworms within 24 h, although it had low toxicity toward houseflies. Thus, a mixture of star anise EO + geranial is a promising source of EO-derived insecticides for housefly control that is also safe for important non-target species.
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Adulticidal activity of star anise, turmeric, cloves and combinations against houseflies
(2025-01-01)
Sinthusiri, J.
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Soonwera, M.
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Puwanard, C.
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Passara, H.
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Thongsaiklaing, T.
The small droplet size could increase the absorbed ingredients and high stability. The star anise nanoemulsion was determined optimum efficacy, with a 1% concentration resulting in the highest knockdown and mortality rate of 100%. Its KT<inf>50</inf> and mortality effective values were 28 min and 36 times, respectively. Furthermore, the adult stage of houseflies is found to be susceptible to star anise nanoemulsion. In contrast, cypermethrin was less toxic to it, with a high KT<inf>50</inf> and resistance to adult houseflies. Consequently, the star anise nanoemulsion could be developed into an efficient and safe environment for controlling housefly populations.
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Antifungal efficacy of stingless bee honey extracts against a black mould causing pathogen Lasiodiplodia spp
(2026-03-01)
Datumada, H.
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Niyomdecha, A.
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Passara, H.
;
Ramdoung, T.
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Sudwisai, W.
Fungal species causing the black sooty mould were isolated from fruit peels and subjected to molecular genetic analysis. The nucleotide sequences were found to be 100% identical to the fungus Lasiodiplodia iranensis. Results from testing the antifungal properties of stingless bee honey extracts showed that the extract from the H. itama species exhibited the highest inhibitory effect, followed by T. pagdeni, G. thoracica, and T. fuscobalteata. The average inhibition values were 21.43+1.27, 18.54+1.50, 17.51+1.76, and 17.18+0.91 mm., respectively.

Passara, Hataichanok

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