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    Item type:Publication,
    Impact of botanical essential oils on pupation and survival of Musca domestica L.
    (2026-05-01)
    Moungthipmalai, T.
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    Sriboonjit, N.
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    Khuendee, P.
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    Phunglakchai, J.
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    Thongsaiklaing, M.
    Among the tested botanical extracts, Illicium verum (star anise) essential oil at 10% demonstrated the highest efficacy in disrupting normal pupal development. Only 65.00% of individuals successfully emerged as fully developed adults after a 10-day exposure period. A notable proportion exhibited transitional deformities, particularly malformed pupal-adult intermediates, indicating interference with metamorphic progression. In comparison, cypermethrin, a commonly applied synthetic insecticide at the same concentration, resulted in a lower mortality rate of 39.67% under identical experimental conditions. The superior performance of star anise oil highlighted its potential as a plant-derived alternative for insect control. These findings suggested that the incorporation of such natural products into integrated pest management strategies may reduce dependence on conventional synthetic chemicals.
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    Item type:Publication,
    Binary Mixtures of Essential Oils: Potent Housefly Adulticides That Are Safe Against Non-Target Species
    (2025-08-01) ;
    Sittichok, Sirawut
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    Moungthipmalai, Tanapoom
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    Murata, Kouhei
    In this study, we investigated the insecticidal potential of Eucalyptus globulus Labill. and Cymbopogon citratus Stapf essential oils (EOs), both alone and in synergistic blends with their primary active compounds, against adult houseflies (Musca domestica L.). Toxicity assessments were also conducted on non-target organisms—dwarf honeybees (Apis florea Fabricius) and guppies (Poecilia reticulata Peters)—to evaluate environmental safety. All binary EO mixtures demonstrated superior efficacy compared to individual EOs and the synthetic pyrethroid α-cypermethrin (1% positive control). The most potent formulation, combining 2.5% (v/v) geranial with 2.5% (v/v) E. globulus EO, exhibited a synergistic effect, achieving complete fly mortality (LT<inf>50</inf>: 0.06 h). This mixture’s mortality index significantly exceeded those of single-component formulations, with a mortality index of 0.22, confirming greater toxicity to flies than α-cypermethrin. Importantly, all the tested EOs and their blends were non-toxic to honeybees and guppies; in comparison, α-cypermethrin caused significant harm. These findings highlight the 2.5% (v/v) geranial + 2.5% (v/v) E. globulus EO blend as a highly effective and environmentally friendly alternative to conventional insecticides. Further research is recommended to optimize its formulation for practical use in sustainable fly management.
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    Item type:Publication,
    Synergistic Adulticidal Activity of Lemongrass (Cymbopogon citratus), Star Anise (Illicium verum), Nutmeg (Myristica fragrans) Essential Oil and Their Components Against the Housefly (Musca domestica) and Their Safety for Key Non-Target Organisms
    (2026-04-01) ; ;
    Moungthipmalai, Tanapoom
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    Murata, Kouhei
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    Botanical insecticides containing a mixture of plant essential oils (EOs) are considered suitable for the management of houseflies (M. domestica). The adulticidal efficacies of single EOs and mixtures of EOs, including lemongrass (C. citratus), star anise (I. verum), nutmeg (M. fragrans), and their components (geranial, trans-anethole, and α-pinene), against houseflies were determined in comparison to 2% (w/v) α-cypermethrin as the positive control and distilled water as the negative control. The mixture of star anise EO (1%) + geranial (1%) was the most effective adulticide, superseding single EOs, other combinations of EOs, and its active component, α-cypermethrin, and distilled water. This mixture was highly synergistic and was found to be over 74% more toxic than all single EOs and almost 2.6 times more toxic than α-cypermethrin. Furthermore, the tested EOs did not cause mortality in guppies (P. reticulata) or earthworms (E. fetida), and caused a maximum of 48% mortality in honeybees (A. mellifera) at 24 h; by contrast, α-cypermethrin led to 100% mortality in honeybees within 0.5 h and in guppies and earthworms within 24 h, although it had low toxicity toward houseflies. Thus, a mixture of star anise EO + geranial is a promising source of EO-derived insecticides for housefly control that is also safe for important non-target species.
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    Item type:Publication,
    Plant essential oils, trans-anethole and eugenol, for housefly knockdown and mortality
    (2025-03-01)
    Sittichok, S.
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    Sinthusiri, J.
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    Thongsaiklaing, T.
    The knockdown and mortality of some compounds from natural oils (trans-anethole, eugenol and a combination of trans-anethole and eugenol) against adult houseflies (Musca domestica) compared with cypermethrin was investigated. The knockdown and mortalities were highest at 100% of 1% trans-anethole with KT50= 31 min and LT50 = 31 min. The mortality index of trans-anethole was 82.1 times that of cypermethrin. In contrast, 1% eugenol had knockdown and mortality was 36% and 0.5% trans-anethole + 0.5% eugenol had knockdown and mortality of 68%, with mortality indexes of 1.6 and 3.2 times versus cypermethrin. The trans-anethole high toxicity and low residue made it a superior control for houseflies.