Passara, Hataichanok

Preferred name

Alternative Name

Passara, H.

Main Affiliation

School of Agricultural Technology

hataichanok.pa@kmitl.ac.th

Now showing 1 - 10 of 24

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Ovicidal Toxicity and Morphological Changes in Housefly Eggs Induced by the Essential Oils of Star Anise and Lemongrass and Their Main Constituents
(2024-07-01)
Passara, Hataichanok
;
Sittichok, Sirawut
;
Sinthusiri, Jirisuda
;
Moungthipmalai, Tanapoom
;
Puwanard, Cheepchanok
This study attempted to evaluate the ovicidal activity of single-component formulations and combination formulations of lemongrass and star anise essential oils (EOs) and their main constituents against housefly eggs. The efficacies of the combinations were compared with those of single-component formulations and α-cypermethrin. Safety bioassays of all treatments and α-cypermethrin on non-target predators—guppy and molly—were conducted. Two combinations: 1% lemongrass EO + 1% trans-anethole and 1% star anise EO + 1% geranial, exhibited a strong ovicidal activity with an inhibition rate of 94.4 to 96.2%. They were 1.1 times as effective as α-cypermethrin. The two combinations also showed high synergistic activity compared to single-component formulations, with a high synergistic index and a high increased inhibition value of 37.4 to 57.7%. All EO treatments were benign for all non-target aquatic species with a high 50% lethal time (LT<inf>50</inf>) and safety index. In contrast, α-cypermethrin was highly toxic to them with a low LT<inf>50</inf>. The morphological abnormalities observed in housefly eggs at death were those such as the shrivelling of the eggs, aberrations and damage to the eggshells, hatching lines, aeropyles, plastron, and micropyle. The potential of these two combinations as a cypermethrin replacement is compelling.
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Evaluating the acaricidal effect of essential oil nanoemulsion against the cutworm, Spodoptera litura
(2022-09-01)
Passara, H.
;
Pumnaun, J.
;
Thipmanee, K.
The cutworm, Spodoptera litura is an important pest of cruciferous vegetables and it causes economic impact worldwide. The efficiency of turmeric and star anise essential oil nanoemulsions (nEOs) against the cutworm, including mortality, antifeedant, and growth inhibition effects was investigated. Essential oil nanoemulsions (nEOs) were prepared by mixing the essential oils with surfactants and co-surfactants. The 2nd, stage instar larvae of cutworms were tested by leaf dipping method and were observed after 24 hours. The results showed that the two nEOs, which were turmeric and star anise, performing the mortality, and growth development effect on Spodoptera litura as well as having antifeedant property. Complete mortality rate was shown at 0.35 % concentration of turmeric and star anise nEOs, when 100% antifeedant effect was found at 0.20% concentrations of nEOs, and 100% growth inhibition was observed at 0.30% concentrations of nEOs. It could be concluded that the nEOs from star anise had antifeedant and insecticide properties.
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Synergistic Larvicidal and Pupicidal Effects of Monoterpene Mixtures Against Aedes aegypti with Low Toxicity to Guppies and Honeybees
(2025-07-01)
Sittichok, Sirawut
;
Passara, Hataichanok
;
Moungthipmalai, Tanapoom
;
Sinthusiri, Jirisuda
;
Murata, Kouhei
The present study evaluated the larvicidal and pupicidal activities of pure and mixed monoterpene formulations—eucalyptol, geranial, trans-anethole, and trans-cinnamaldehyde—against Aedes aegypti and compared them with 1% (w/w) temephos. Safety bioassays of all formulations on non-target species confirmed their safety. The combined mixture of eucalyptol + trans-anethole at 400 µg/mL exhibited stronger larvicidal activity, with an LC<inf>50</inf> of 176 µg/mL, while the combination of trans-anethole + geranial at 400 µg/mL exhibited stronger pupicidal activity with an LC<inf>50</inf> of 167 µg/mL. Both formulations were more effective than a 1% temephos. All the mixture formulations were more strongly synergistic compared to pure formulations, with an increased mortality value (IMV) of 25% to 95%. External morphological aberrations observed at death included swelling of the respiratory system. Importantly, all the formulations were safe for two non-target species: guppies (Poecilia reticulata) and honeybees (Apis mellifera). The combination formulations are strong larvicides and pupicides for controlling Ae. Aegypti, which will help reduce the spread of viruses carried by this vector.
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Impact of botanical essential oils on pupation and survival of Musca domestica L.
(2026-05-01)
Moungthipmalai, T.
;
Sriboonjit, N.
;
Khuendee, P.
;
Phunglakchai, J.
;
Thongsaiklaing, M.
Among the tested botanical extracts, Illicium verum (star anise) essential oil at 10% demonstrated the highest efficacy in disrupting normal pupal development. Only 65.00% of individuals successfully emerged as fully developed adults after a 10-day exposure period. A notable proportion exhibited transitional deformities, particularly malformed pupal-adult intermediates, indicating interference with metamorphic progression. In comparison, cypermethrin, a commonly applied synthetic insecticide at the same concentration, resulted in a lower mortality rate of 39.67% under identical experimental conditions. The superior performance of star anise oil highlighted its potential as a plant-derived alternative for insect control. These findings suggested that the incorporation of such natural products into integrated pest management strategies may reduce dependence on conventional synthetic chemicals.
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Adulticidal synergy of two plant essential oils and their major constituents against the housefly Musca domestica and bioassay on non-target species
(2024-03-15)
Soonwera, Mayura
;
Moungthipmalai, Tanapoom
;
Puwanard, Cheepchanok
;
Sittichok, Sirawut
;
Sinthusiri, Jirisuda
Single and mixture formulations of lemongrass (Cymbopogon citratus (DC.) Stapf.) and star anise (Illicium verum (J. Presl.)) essential oils (EOs) and their major constituents were assayed for their adulticidal activities against housefly, Musca domestica L., and two non-target species, stingless bee (Tetragonula pegdeni Schwarz) and guppy (Poecilia reticulata Peters). The efficacies of the mixture formulations were compared against those of the single formulations and 1.0% α-cypermethrin, a common synthetic insecticide. GC-MS analysis found that the major constituent of lemongrass EO was geranial (45.23%), and that of star anise EO was trans-anethole (93.23%). Almost all mixture formulations were more effective in adulticidal activity against housefly adults than single formulations and 1.0% α-cypermethrin. A mixture of 1.0% lemongrass EO + 1.0% trans-anethole exhibited the strongest synergistic insecticidal activity with a 100% mortality rate (KT<inf>50</inf> of 3.2 min and LT<inf>50</inf> of 0.07 h). The relative percentage increase in mortality rate over single formulations was between 1.6 and 91.9%. In addition, it was three times more effective than 1.0% α-cypermethrin. To find the mechanism of adulticidal action, scanning electron microscopy (SEM) was done to find morphological aberrations, such as antennal and mouthpart aberrations, after the houseflies were treated with 1.0% lemongrass EO + 1.0% trans-anethole. The aberrations included deformed and abnormal shape of arista and flagellum, change in labellum pigmentation, and damage to pseudotracheae. Regarding toxicity against non-target species, all single and mixture formulations were not toxic to the two non-target species, while 1.0% α-cypermethrin was highly toxic. To conclude, a mixture of 1.0% lemongrass EO + 1.0% trans-anethole can be an excellent, natural, sustainable housefly adulticidal agent.
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Head lice prevalence in suburban Bangkok, Thailand, after the COVID-19 pandemic and herbal shampoo treatment
(2026-01-01)
Sinthusiri, J.
;
Sittichok, S.
;
Passara, H.
;
Soonwera, M.
The prevalence of pediculosis among 3,461 girls from 10 government kindergartens and primary schools in Ladkrabang district was 48.1% in the 1st semester (May-September 2022) and 44.6% in the 2nd semester (November 2022-March 2023), higher among primary school girls, at 51.8-55.4%, than in kindergarteners, at 30.2-33.1%. Over 80% of the infested girls came from a low-income family (<3,000 USD per year). The Zingiberaceae essential oil and shampoo treatments were highly effective with 100% cure rate for every infested girl after the 2nd treatment (on Day 10, after the 1st treatment on Day 1). Effective strategies for reducing head lice prevalence are improved living standards and head lice eradication by treatments with effective natural pediculicides and improved head lice educational program.
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Combinations of Lemongrass and Star Anise Essential Oils and Their Main Constituent: Synergistic Housefly Repellency and Safety against Non-Target Organisms
(2024-03-01)
Soonwera, Mayura
;
Sinthusiri, Jirisuda
;
Passara, Hataichanok
;
Moungthipmalai, Tanapoom
;
Puwanard, Cheepchanok
The present study evaluated the housefly repellency of single-component formulations and combinations of lemongrass and star anise essential oils (EOs) and their main constituents. The efficacies of the combinations were compared against those of single-component formulations and DEET. Safety bioassays of all formulations and DEET on non-target species—guppy, molly, dwarf honeybee, and stingless bee—were conducted. GC–MS analysis showed that the main constituent of lemongrass EO was geranial (46.83%) and that of star anise EO was trans-anethole (92.88%). All combinations were highly synergistic compared to single-component formulations, with an increased repellent value (IR) of 34.6 to 51.2%. The greatest synergistic effect was achieved by 1.0% lemongrass EO + 1.0% trans-anethole combination, with an IR of 51.2%. The strongest, 100% repellent rate at 6 h was achieved by 1.0% geranial + 1.0% trans-anethole. They were twice as effective as DEET and caused obvious damage to housefly antennae under microscopic observation. All single-component formulations and combinations were benign to the four tested non-target species. In contrast, DEET was highly toxic to them. The synergistic repellency and biosafety of these two combinations are compellingly strong support for developing them into an effective green repellent.
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Binary Mixtures of Essential Oils: Potent Housefly Adulticides That Are Safe Against Non-Target Species
(2025-08-01)
Passara, Hataichanok
;
Sittichok, Sirawut
;
Moungthipmalai, Tanapoom
;
Laosinwattana, Chamroon
;
Murata, Kouhei
In this study, we investigated the insecticidal potential of Eucalyptus globulus Labill. and Cymbopogon citratus Stapf essential oils (EOs), both alone and in synergistic blends with their primary active compounds, against adult houseflies (Musca domestica L.). Toxicity assessments were also conducted on non-target organisms—dwarf honeybees (Apis florea Fabricius) and guppies (Poecilia reticulata Peters)—to evaluate environmental safety. All binary EO mixtures demonstrated superior efficacy compared to individual EOs and the synthetic pyrethroid α-cypermethrin (1% positive control). The most potent formulation, combining 2.5% (v/v) geranial with 2.5% (v/v) E. globulus EO, exhibited a synergistic effect, achieving complete fly mortality (LT<inf>50</inf>: 0.06 h). This mixture’s mortality index significantly exceeded those of single-component formulations, with a mortality index of 0.22, confirming greater toxicity to flies than α-cypermethrin. Importantly, all the tested EOs and their blends were non-toxic to honeybees and guppies; in comparison, α-cypermethrin caused significant harm. These findings highlight the 2.5% (v/v) geranial + 2.5% (v/v) E. globulus EO blend as a highly effective and environmentally friendly alternative to conventional insecticides. Further research is recommended to optimize its formulation for practical use in sustainable fly management.
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Impact of Nigrospora oryzae-Derived Natural Products on Photosynthesis and Oxidative Stress in Eichhornia crassipes
(2025-01-01)
Laosinwattana, Chamroon
;
Manichart, Nutcha
;
Wichittrakarn, Pattharin
;
Yoneyama, Kaori
;
Teerarak, Montinee
Interest in natural herbicides has been growing due to government policies restricting synthetic herbicide use in many countries. In that regard, this study investigates the potential of Nigrospora oryzae extract as a natural herbicide against the aquatic invasive weed Eichhornia crassipes. A stable formulation was developed with a droplet size of 36.44 ± 0.36 nm and a zeta potential of -62.59 mV. Pot-based experiments revealed the N. oryzae extract induced 38.33% phytotoxicity within 24 hours, increasing to 84.72% by 28 days post-treatment. Scanning electron microscopy demonstrated morphoanatomical changes in epidermal tissue and stroma of E. crassipes, such as erosion of epicuticular waxes and degeneration of epidermis cells. The treatment significantly reduced the photosynthetic pigment content while increasing hydrogen peroxide (46.26%), malondialdehyde (17.49%), and proline (19.16%) levels, causing cellular electrolyte leakage. Activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase were significantly elevated (p<0.05), indicating oxidative damage. These findings demonstrate that N. oryzae extract can disrupt growth and photosynthesis and induce oxidative stress in E. crassipes, suggesting its potential as a source of natural herbicide for industrial application.
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The effectiveness of star anise nanoemulsion and chemical insecticide for controlling of beet armyworm
(2024-05-01)
Passara, H.
;
Pumnuan, J.
;
Thongsaiklaing, T.
;
Thipmanee, K.
The effectiveness of star anise (Illicium verum) nanoemulsion and deltamethrin (chemical insecticide) was tested with beet armyworm (Spodoptera exigua) to evaluate their mortality, antifeedant, and growth inhibition effects at proper concentrations. As a result, at the obtained highest effectiveness, star anise nanoemulsion required 0.35% concentration for the maximum effectiveness with 100% mortality, antifeedant and growth inhibition effects against beet armyworm, when compared with the control (Tween20 and water), while deltamethrin exhibited a lower rate. Therefore, star anise nanoemulsion seem to be utilized as a highly effective and environmentally friendly insecticide to control beet armyworm.

Passara, Hataichanok

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