Aue-Umnoey, Dusit

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Aue-Umnoey, Dusit
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Aueumneoyyyyy, Dusit
Aue-umneoy, Dusit
Aueumneoy, Dusit
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dusit.au@kmitl.ac.th

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    Use of a filamentous green alga (Chaetomorpha sp.) and microsnail (Stenothyra sp.) as feed at an early stage of intensive aquaculture promotes growth performance, artificial feed efficiency, and profitability of giant tiger prawn (Penaeus monodon)
    (2020-12-01)
    Tsutsui, Isao
    ;
    Aueumneoy, Dusit
    ;
    Pinphoo, Piyarat
    ;
    Thuamsuwan, Worachet
    ;
    Janeauksorn, Kittipong
    With the worldwide demand for tropical penaeid prawn increasing in recent decades, more research on shrimp culture methods is needed to enhance efficiency and profitability for shrimp farmers. The objective of this study was to develop a technique to boost the productivity, feed efficiency, and profitability of the giant tiger prawn (Penaeus monodon). To accomplish this, a novel culture setup was established in which two benthic organisms, a filamentous green alga (Chaetomorpha sp.) and a microsnail (Stenothyra sp.), were propagated together with P. monodon post-larvae during an early culture stage and then offered to shrimp as supplementary live feeds in intensive aquaculture ponds. For the experiment, shrimp post-larvae (density: approximately 33 individuals m<sup>-2</sup>) were cultured in outdoor concrete ponds (9 × 9 × 1.2 m) under either control (fed only artificial feed, n = 3) or experimental (fed artificial feed and benthic organisms, n = 3) conditions until they reached marketable size (15 weeks). Apparent green algae consumption was 6.81 kg (8.4% green alga to total feed consumption), whereas microsnail consumption was 1.96 kg (2.4% microsnail to total feed consumption). Compared with the control group of giant tiger prawn, the experimental group showed significantly higher productivity (total number of shrimp produced: 118%; total shrimp production: 133%), feed efficiency (feed conversion ratio of artificial shrimp feed: 89%), and profitability (shrimp sales: 139%; balance between shrimp sales and costs: 146%), while labor and financial costs were kept minimal. These results can be explained by the enhanced growth of shrimp at the early stages of culture. The techniques developed in this study will help to advance the efficiency of intensive aquaculture operations for giant tiger prawn and also improve profitability for shrimp farmers.
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    Cultivation of Wolffia globosa and its application in functional food development
    (2025-12-01)
    Sirison, Jiraporn
    ;
    Ruangsomboon, Suneerat
    ;
    Jongput, Buppha
    ;
    Aue-umneoy, Dusit
    ;
    Tongsri, Pajongjit
    This study assessed the biomass productivity, biochemical composition, and functional food potential of Wolffia globosa cultivated in four nutrient media: AB hydroponic solution, Chlorella medium, 16-16-16 fertilizer, and 16-16-16 supplemented with a vitamin B complex and FeSO<inf>4</inf> (16-16-16-B). Cultivation was conducted for eight weeks, with biomass harvested weekly. Among the treatments, the Chlorella medium produced the greatest biomass yield (133.82–236.00 g FW tank⁻<sup>1</sup>) and daily productivity (23.90–42.14 g FW m⁻<sup>2</sup> day⁻<sup>1</sup>), significantly surpassing the other media (p < 0.05), followed by 16-16-16-B. Medium replenishment at four weeks initially enhanced growth but was later accompanied by a decline in biomass. Biochemical analysis indicated that the Chlorella medium yielded the highest protein content (46.10 ± 0.93% DW), while the AB medium supported the greatest chlorophyll-a (4.08 ± 0.00% DW) and carbohydrate levels (37.21 ± 1.12% DW); all differences among treatments were statistically significant (p < 0.05). Considering cost-effectiveness and nutritional value, the 16-16-16-B medium was optimal for cultivating W. globosa for food applications. Dried biomass from this medium was incorporated into fresh pasta and fried sweet potato balls, significantly enhancing chlorophyll-a, carotenoids, protein, fiber, and calcium contents. Sensory evaluation using a 9-point hedonic scale showed strong consumer acceptance even at higher inclusion levels. These findings highlight the role of nutrient-enriched media, particularly 16-16-16-B, in enhancing biomass yield and nutritional quality of W. globosa, affirming its potential as a functional food ingredient. Beyond nutritional enhancement, W. globosa-based foods exhibit immunomodulatory and anticancer potential through the antioxidant activity of pigments and other bioactive compounds.
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    Assessment of Bacterial Community and Other Microorganism Along the Lam Takhong Watercourse, Nakhon Ratchasima, Thailand
    (2025-06-01)
    Klangnurak, Wanlada
    ;
    Hinthong, Woranich
    ;
    Aue-umneoy, Dusit
    ;
    Yomla, Rungtawan
    Lam Takhong, a vital watercourse in Nakhon Ratchasima province, Thailand, supports agricultural, recreational, and urban activities. Originating in a national park, it flows through urban areas before discharging into a dam and running off via the sluice gate. While water quality monitoring is routine, microbial community data have never been reported. This study assesses the microorganism diversity and functional genes in Lam Takhong watercourse using a shotgun sequencing metagenomics approach. Water samples were collected from the upstream, midstream, and downstream sections. The midstream area exhibited the highest abundance of fecal coliform bacteria, plankton, and benthos, suggesting elevated pollution levels. Genes related to metabolism, particularly carbohydrate and amino acid pathways, were predominant. Proteobacteria was the most abundant phylum found in the water, with Limnohabitans as the dominant planktonic bacteria. Bacteria such as Staphylococcus, Mycobacterium, Escherichia, Pseudomonas, Enterococcus, Neisseria, Streptomyces, and Salmonella were detected, along with antibiotic resistance genes, raising public health concerns. These findings emphasize the need for microbial monitoring in the Lam Takhong to determine the potential water quality bioindicator and prevent potential disease spread through the water system.
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    Utilization of micro-snail Stenothyra species as a co-feed for giant tiger prawn
    (2026-06-01)
    Aue-umneoy, Dusit
    ;
    Pinphoo, Piyarat
    ;
    Thuamsuwan, Worachet
    ;
    Janeauksorn, Kittipong
    ;
    Meethong, Grissada
    Giant tiger prawn (Penaeus monodon) is one of the most commercially important species in global intensive shrimp aquaculture. However, rising fishmeal prices have driven increases in feed costs, prompting some farmers to use lower-quality feeds, which can negatively affect shrimp growth performance. Natural micro-snails, such as Stenothyra sp., have been identified as potential co-feeds for P. monodon. Therefore, this study aimed to investigate the effects of Stenothyra sp. as a co-feed on the growth performance and feed efficiency of P. monodon. Juvenile shrimp were assigned to two treatment groups (n = 20 per group): control (fed artificial pellets) and co-feed (fed artificial pellets supplemented with live micro-snails). The specific growth rate was higher in the co-feed group (8.4 ± 0.11% per day) than that in the control group (7.2 ± 0.34% per day). The final body weight was approximately 61% higher in the co-feed group than that in the control group. Similarly, feed efficiency (119.6 ± 2.73% vs. 77.5 ± 2.90%) and total protein intake (6.8 ± 0.17 g vs. 1.7 ± 0.06 g) were significantly higher in the co-feed group than those in the control group, indicating better feed and nutrient utilization following Stenothyra sp. supplementation. In conclusion, under the tested conditions, providing Stenothyra sp. as a co-feed improved growth performance and feed efficiency in juvenile P. monodon, possibly due to increased total protein intake. Overall, this study presents an innovative co-feeding strategy with potential benefits; however, its broader practical and economic implications require further evaluation.