Prachayawarakorn, Jutarat

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Asymmetric dot-patterned wettable and antibacterial wound dressings from bacterial cellulose–alginate composites coated with stearic acid-modified ZnO/chitosan/AgNPs
(2025-01-01)
Ieamviteevanich, Pimchanok
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Onklam, Panida
;
Kampechdee, Wariya
;
Churiwan, Achana
;
Vittayakorn, Naratip
To improve the wound dressing characteristics of bacterial cellulose-based materials and address the issue of asymmetric wound dressing with one hydrophilic side and another hydrophobic side, this study developed a new concept for the fabrication of an asymmetric wettable and antibacterial wound dressing by selective drop coating of stearic acid-modified ZnO, chitosan, and AgNPs to form a dot pattern on both surfaces of a bacterial cellulose–alginate composite (BA-ZnS/Ch/Ag). The coated surface was hydrophobic, with a WCA of 150° due to the formation of a low surface energy zinc stearate (C<inf>17</inf>H<inf>35</inf>COO)<inf>2</inf>Zn) monolayer on the ZnO particles and a high degree of hierarchical roughness. The asymmetric wettable BA-ZnS/Ch/Ag wound dressing maintained good water absorptivity (swelling rate 417%) and natural breathability (water vapor transmission rate 792 g.m−2 day−1) of the superhydrophilic bacterial cellulose-alginate composite that consisted of dense outer surfaces and porous inner layers and simultaneously possessed the superhydrophobic property of the coating area that can reduce the risk of infection from external fluids and improve the blood repellency and anti-adhesion properties. The BA-ZnS/Ch/Ag wound dressing showed good antibacterial activity against S. aureus and E. coli and non-toxicity to human keratinocyte immortal cells (HaCaT), making it suitable for clinical applications.
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Smart Antibacterial Sponge Based on Basil Seed Mucilage/Agar/Mandelic Acid with pH Responsive Feature by Butterfly Pea Extract
(2026-01-01)
Subyai, Natkitta
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Pitpisutkul, Vipawan
;
Kruta, Pannita
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Phonrat, Arnon Sai anan
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Prachayawarakorn, Jutarat
The development of antimicrobial wound dressings is critical for enhancing wound healing and preventing infections. This study presented a novel antimicrobial sponge composed of both basil seed mucilage and agar with the addition of mandelic acid as an antibacterial agent, including the incorporation of butterfly pea extract as a pH sensitive substance. The sponges were fabricated through a freeze-drying process, using succinic acid as a crosslinking agent. The functional group analysis offered the infrared-shifted peak position of O-H stretching and O-H bending in basil seed mucilage/agar/succinic acid sponges with the addition of mandelic acid and the extract, indicating of hydrogen bond formation among the crosslinked natural polymers, mandelic acid and the extract. The morphology of the crosslinked sponges incorporated mandelic acid and the extract revealed the opened-cell structures with interconnected cell walls. The crosslinked antibacterial sponges greatly promoted their mechanical properties and gel fraction. The inclusion of mandelic acid did not change the porosity or water retention of the sponges, but the addition of the extract enhanced their swelling behavior. While mandelic acid improved antibacterial activity against both Staphylococcus aureus and Escherichia coli; the extract, provided notable pH-responsive characteristics. The color measurement and cytotoxicity assay were also examined.
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Valorization of eggshell waste to metal-doped CaO catalysts for producing dimethyl carbonate by transesterification of propylene carbonate with methanol
(2026-05-01)
Seeharaj, Panpailin
;
Werupho, Sakila
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Puyamsai, Araya
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Choengchan, Nathawut
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Kim-Lohsoontorn, Pattaraporn
To align with a circular economy, this study aimed to extend the lifecycle of eggshell waste by valorizing it into a catalyst for producing the green chemical dimethyl carbonate (DMC) through the transesterification of propylene carbonate (PC) with methanol. CaO derived from eggshells was modified by doping with 10 mol% of aliovalent metal cations, including Na+, Mg2+, and Ce3+, via a calcination-hydration-dehydration process. The catalyst activity was tested under atmospheric air at 40–70 °C for 1–3 h. Metal-doped CaO possessed better activity than pure CaO, and the performance was ranked in order as CaO < Na–CaO < Ce–CaO < Mg–CaO. At the optimized reaction conditions of 50 °C for 2 h, the Mg–CaO catalyst exhibited the best performance, with 77% PC conversion, 62% DMC selectivity, and 37% DMC yield. This improvement correlated with the high surface-active area and optimum basicity induced by incorporating Mg2+ into the CaO structure and forming MgO–CaO mixed oxide phases. This study demonstrated a greener process for producing DMC under mild conditions using metal-doped CaO catalysts derived from eggshell waste.
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Alternative Polysaccharide Wound Dressing from Heat-Moisture Starch/Basil Seed (O. Basilicum L.) Mucilage with Incorporated Glycolic Acid Antimicrobial Agent
(2025-01-01)
Phonrat, Arnon
;
Subyai, Natkitta
;
Prachayawarakorn, Jutarat
In order to improve the behavior in water and the mechanical properties of heat-moisture treated starch, natural basil seed mucilage with high hydrophilicity and crystallinity was incorporated for wound dressing application. In addition, succinic acid cross-linking was used to enhance the durability of the material in water and glycolic acid was utilized as an antimicrobial agent. The effectiveness of the cross-linking process could be observed from the significant increase in dimensional stability from higher gel fraction value and increment of mechanical properties. Furthermore, the incorporation of glycolic acid into the wound dressing films demonstrated antibacterial efficacy, resulting in 100% bacterial reduction against S. aureus and E. coli strains. These films exhibited low cytotoxicity toward human epidermal keratinocytes (HaCaT) cells, indicating their potential for biomedical applications. Moreover, functional group analysis, crystallinity, morphology, thermal properties, gel fraction, water vapor transmission rate, free swell absorption, and mechanical properties were also examined.

Prachayawarakorn, Jutarat

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