Worananthakij, Worakrit
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Item type:Publication, Morphological and genetic characterization of marine filamentous cyanobacterium Geitlerinema isolated from Thailand(2018-04-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Microwave-assisted biosynthesis of silver nanoparticles using two marine microalgal extracts and their antimycobacteriosis activity against bacteria isolated from Betta splendens(2025-12-01); ; ; This study explores the synthesis and antibacterial properties of silver nanoparticles (AgNPs) as a safer, eco-friendly alternative to traditional chemical treatments for bacterial infections. AgNPs were synthesized using aqueous extracts of marine microalgae, Isochrysis galbana and Chaetoceros calcitrans, via conventional and microwave-assisted methods, with the latter accelerating nanoparticle production. Extracts in ethanol, hexane, and acetone were tested, with the ethanolic extract of I. galbana showing the strongest antibacterial effects. The AgNPs exhibited broad-spectrum antibacterial activity against pathogens such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and fish pathogens like Aeromonas veronii. Microwave-assisted synthesis with ethanolic extracts resulted in the highest inhibition, particularly against fish and tuberculosis-related pathogens, including Mycobacterium marinum. Nanoparticle formation was confirmed using various characterization methods, including ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), which revealed crystalline structures. Transmission electron microscopy (TEM) analysis revealed that AgNPs varied in size, with an average diameter of less than 50 nm and all particles being smaller than 100 nm. This research demonstrates the potential of AgNPs as an effective alternative to antibiotics, offering targeted bacterial inhibition while reducing the risk of antibiotic resistance. This makes it a promising approach for treating bacterial infections in ornamental fish. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Occurrence of Parasitic and Bacterial Pathogen in Ornamental and Wild Populations of Siamese Fighting Fish (Betta splendens) in a Region of Thailand(2025-05-01); The diversity of fish parasites reflects the diversity of parasites in the water source, providing insights into the effects of pathogens and essential information about parasite-host relationships. Parasitic infections are valuable indicators of the aquatic ecosystem, influenced by various factors. This study aimed to investigate the prevalence and seasonality of parasite species and pathogenic bacteria in Siamese fighting fish (Betta splendens) over 12 months, involving sixty ornamental Siamese fighting fish collected from commercial sources and 81 wild Siamese fighting fish from different natural habitats. Parasite prevalence in ornamental Siamese fighting fish (25.00%) was lower than in the wild (34.57%). The protozoa parasite Trichodina was found in both Siamese fighting fish, with the highest prevalence recorded for Trichodina sp. in wild Siamese fighting fish. The winter season exhibited the highest parasitic prevalence, with a tremendous diversity of parasites found at each location, followed by the rainy and summer seasons. This study also reported the first finding of Henneguya sp. infection in Siamese fighting fish and on the body surface. The prevalence and seasonality of parasite genera were significant in the wild compared to ornamental Siamese fighting fish. Bacterial isolation was performed on internal organs, and isolates were identified using PCR techniques. Aeromonas veronii and Mycobacterium marinum were detected in ornamental Siamese fighting fish, while A. veronii was found in wild Siamese fighting fish. These findings indicate that infections in Siamese fighting fish display seasonal variation and are impacted by their ecology. This information is fundamental for managing the biodiversity of parasites in fish and preventing parasite infections in aquaculture. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Microwave-Assisted Biosynthesis of Silver Nanoparticles Using Chlorella sp. for Antibacterial and Cytotoxicity Effects of Breast Cancer Cell Line(2026-03-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antibacterial activity of marine microalgae extracts against pathogen of Siamese fighting fish (Betta splendens)(2025-01-01); Background: Marine microalgae extracts are a promising alternative to commercial drugs because of their antimicrobial properties. Aim: This study aimed to investigate the antibacterial properties of crude extracts containing bioactive compounds derived from Chlorella sp., Isochrysis galbana, and Chaetoceros calcitrans. Methods: Crude extracts from microalgae were obtained via maceration using ethanol and hexane as solvents. Antibacterial activities were initially screened using the agar well diffusion method, whereas antimycobacterial activity was assessed using the microplate 7H11 agar proportion method. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the crude extracts were determined. Gas chromatographymass spectrometry (GC-MS) was used for phytochemical composition analysis to identify significant antimicrobial compounds in the extracts. Results: The ethanolic extract of I. galbana demonstrated detectable antibacterial activity, with an inhibition zone of 12.40 ± 0.06 mm against Bacillus subtilis, 12.22 ± 0.28 mm against Staphylococcus aureus, 11.10 ± 0.11 mm against Pseudomonas aeruginosa, and 10.99 ± 0.33 mm against Aeromonas veronii at 100 mg/ml. Extracts from I. galbana and C. calcitrans inhibited Mycobacterium marinum, suggesting potential benefits for betta fish health. MIC values were 25 mg/ml for A. veronii in the ethanolic extracts of I. galbana, C. calcitrans, Chlorella sp., and C. calcitrans hexane extract. The GC-MS chromatogram of the ethanolic extract of I. galbana revealed the presence of several bioactive compounds. The major components identified include Phthalate, Tetradecanoic acid, Hexadecanoic acid, and Phytol. Conclusion: This study demonstrated the promising antimicrobial potential of crude extracts from marine microalgae, particularly the ethanolic extract of I. galbana, which showed strong activity against both terrestrial and aquatic pathogens. The findings suggest that bioactive compounds in these extracts, especially those from I. galbana and C. calcitrans, can be effective in managing bacterial infections such as A. veronii and M. marinum in betta fish. These results highlight the potential of marine microalgae as natural, sustainable alternatives for disease control in aquaculture.