Now showing 1 - 10 of 34
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    A scanner-based microplate platform for high-throughput oxytetracycline detection using diazotization-coupling chemistry with greenness evaluation
    (2026-06-01)
    Puttasa, Papawarin
    ;
    Ngernpimai, Sawinee
    ;
    Tippayawat, Patcharaporn
    ;
    Park, Myoung‑Hwan
    ;
    Routine oxytetracycline (OTC) monitoring of aquaculture effluents, water sources, and pharmaceutical products is essential for environmental management and regulatory compliance. However, existing analytical methods remain largely instrumental and low-throughput. Herein, a microvolume, high-throughput, scanner-based colorimetric microplate platform is developed for OTC determination using a sensitive and selective diazotization-coupling chemistry with RGB digital image analysis. Sulfanilamide is diazotized with nitrite to generate reactive diazonium species, which subsequently couple with OTC to form a strongly colored yellow-orange azo dye. Only microliter-scale volumes of reagents and samples are required in each well, and the reaction is completed within a few minutes, enabling simultaneous analysis of up to 96 samples. The microplate is imaged with a flatbed scanner, and color responses are quantified using ImageJ software. A linear calibration range of 1 − 40 μM was obtained with a detection limit of 0.33 μM. The platform was successfully applied to OTC determination in aquaculture effluents, tap water, drinking water, and pharmaceutical samples. It exhibited high selectivity against other antibiotics with no observable cross-reactivity. The green analytical procedure index (GAPI) confirmed the method’s environmental friendliness. Overall, the scanner-based microplate platform offers a simple, low-cost, portable, and high-throughput alternative to conventional OTC assays.
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    Poly(vinyl alcohol) capped silver nanoparticles for antioxidant assay based on seed-mediated nanoparticle growth
    (2017-08-01) ;
    Jinnarak, Amornrassamee
    ;
    Chaneam, Sumonmarn
    ;
    Wilairat, Prapin
    ;
    Nacapricha, Duangjai
    A simple and rapid method for measurement of total antioxidant capacity (TAC) was developed. In this work, gallic acid was used as the antioxidant standard. Poly(vinyl alcohol) embedded silver nanoparticles (PVA-AgNPs) were employed as a colorimetric sensor. The detection principle was based on the seed-mediated nanoparticle growth technique. The PVA-AgNPs act as a catalyst in the reduction of Ag<sup>+</sup> by gallic acid by providing nucleation seeds. Ag<sup>+</sup> was reduced to Ag° and accumulated on the PVA-AgNP surface, leading to an increase in the size of particles. The absorbance of the colloidal solution was drastically enhanced with a small red shift. Under optimal conditions, a linear response was established between the change in absorbance and the TAC value expressed in terms of gallic acid equivalents. The linear range was from 25 to 200 μM with a detection limit of 22.1 μM. Satisfactory precision was obtained with % relative standard deviation (RSD) of 2.17. The developed sensor was successfully applied for TAC assessment of commercial ginger products. The PVA-AgNP sensor offers rapid analysis (within 5 min) compared to other nanoparticle-based antioxidant assays. Synthesis of the particles and assay involved less-toxic chemicals, and is therefore a “greener” method.
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    Measurement of sucrose concentration using Imbibition length on paper: A device for equipment-free and environmentally-friendly detection
    (2024-04-01)
    Sitanurak, Jirayu
    ;
    Kumpong, Anongnat
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    Yaimai, Orawan
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    Wilairat, Prapin
    ;
    The Lucas-Washburn equation is commonly used to predict the distance (L) that a liquid travels through paper. This equation establishes that L<sup>2</sup> is linear with time and inversely proportional to the viscosity of the liquid. However, there is currently no theoretical equation connecting the viscosity of a solution to its concentration. In this study, the imbibition flow of a sucrose solution was measured along the length of a horizontal strip of filter paper, featuring a printed, thermometer-shaped hydrophobic boundary. A sample (38 μL) was dispensed onto the bulb area, and the solution's flow was visually tracked using a red dye added to the sample. The imbibition length (L) was measured by a vernier caliper at 10.0 min after the sample addition. An empirical equation, based on literature values of the viscosity (η) and concentration (C) of sucrose solutions, was proposed. By integrating this empirical equation with the Lucas-Washburn equation, the following equation was derived: L = a⋅exp{-(bC + cC<sup>2</sup>)}, where ‘a’, ‘b’ and ‘c’ are parameters. This equation was fitted to the dataset of L and C, covering C values from 0 to 60 % w/w standard sucrose solutions, resulting in a coefficient of determination of 0.9987. The plot of L against C was observed to closely follow a linear line, with a fitting providing a coefficient of determination of 0.9986. The sucrose contents in samples, such as soft drinks, syrups, and sugarcanes, determined using the imbibition length method and conventional refractometry, were in statistical agreement via the paired t-test at the 95 % confidence level. This method is simple, instrument-free, requiring only a small amount of safe red food dye, and can be conducted on-site.
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    Photoregulation of Gold Nanoparticles Stabilized in a Diacetylenic Nanocapsule
    (2017-01-01)
    Somjid, Saowalak
    ;
    Chompoosor, Apiwat
    ;
    Kanokmedhakul, Somdej
    ;
    The results of releasing a drug in a burst are unpredictable and one of the inherent drawbacks of using nanocarriers. Here, photoresponsive cationic gold nanoparticles to stabilize diacetylenic nanocapsules enabling photoregulated release of payloads are reported. The fabrication of these nanocapsules relied on an electrostatic interaction of a negatively charged diacetylenic core and a positively charged gold nanoparticle shell. Gold nanoparticles with photoresponsive ligands on their surfaces act as both hydrophobic core stabilizers and gatekeepers of the nanocapsules, while their polydiacetylene cores serve as hydrophobic drug carriers that can be tuned using UV irradiation. The morphology of nanocapsules was analyzed using TEM and dynamic light scattering. The resultant nanocapsules had a spherical shape with an average diameter of 152.9 ± 6.7 nm. Upon UV irradiation, the nanocapsules lost their integrity and an encapsulated model compound was released through diffusion. The release of a hydrophobic molecule was irradiation time dependent and thereby controllable. This light-triggered release provides an alternative strategy for controlled drug delivery.
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    Smartphone-based disposable cotton-swab sensor loaded with creatinine/Cu–chlorophyllin–stabilized AuNPs for ultrasensitive RGB colorimetric detection of mercury (II) ions in aquatic environments
    (2026-05-01)
    Lerdpiriyaskulkij, Natee
    ;
    ; ;
    Chansai, Sarayute
    ;
    A simple, rapid, selective, and sensitive colorimetric sensor based on a disposable cotton swab loaded with Au nanoparticle–modified Cu-chlorophyllin (Au<sup>0</sup>–NPs<inf>CHL</inf>) and creatinine was developed for mercury (II) detection in aquatic environments using smartphone–based RGB analysis. Au<sup>0</sup>–NPs<inf>CHL</inf> were synthesized by ultrasonic-assisted chemical reduction, employing Cu-chlorophyllin as a stabilizing and NaBH<inf>4</inf> as a reducing agent. A vivid red Au<sup>0</sup>–NPs<inf>CHL</inf> colloidal (7.96 ± 0.29 nm) with a sharp SPR peak at 515 nm was successfully obtained. An aliquot of 75 µL of Au<sup>0</sup>-NPs<inf>CHL</inf> (0.103 ± 0.03 nmol L<sup>−1</sup>) and 25 µL of creatinine solution (40 mg L<sup>−1</sup>) were sequentially loaded into an 8.0 × 0.5 cm cotton swab, separated by a 0.5 cm air gap. Detection began by immersing the swab into Hg<sup>2+</sup>–contaminated samples for 3 min, allowing Hg<sup>2+</sup> adsorption. Breaking the swab generated pressure differential, which, along with gravity, drove the Au<sup>0</sup>–NPs<inf>CHL</inf> to mix with creatinine. The resulting mixture migrated toward the swab tip by capillary action and reacted with adsorbed Hg<sup>2+</sup> through metallophilic 5d<sup>10</sup>–5d<sup>10</sup> interactions. Creatinine acted as a bridging ligand, inducing Au<sup>0</sup>–NPs<inf>CHL</inf> aggregation and shifting the SPR to 620 nm, causing a visible red-to-blue transition. This sensor enables rapid and visual detection and quantitative evaluation via smartphone RGB analysis. The system demonstrated excellent linearity from 1 to 100 µg L<sup>−1</sup> with a low LOD of 0.82 µg L<sup>−1</sup>. Recoveries of 97.6–101.8 % confirmed high accuracy and minimal matrix interference. The disposable cotton swab RGB sensor provides a rapid, portable, and practical tool for on-site primary assessment of Hg<sup>2+</sup> contamination in environmental samples.
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    A silver nanoparticle thin film modified glass substrate as a colourimetric sensor for hydrogen peroxide
    (2015-11-22) ;
    Sani, M.
    ;
    Numsawat, P.
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    Martchoo, R.
    ;
    Chompoosor, A.
    In this work, a silver nanoparticle (AgNP) coated glass slide was developed as a device for sensing hydrogen peroxide. AgNPs were synthesised using borohydride reduction with a citrate stabiliser, resulting in a negatively charged stabilised particle surface. The particles were attached to the glass surface using the layer-by-layer (LbL) technique. Poly (diallyldimethylammonium chloride) and poly (styrene sulphonate) were used as cationic and anionic polyelectrolyte layers, respectively. The glass slide was modified with polyelectrolytes leaving a cationic layer on the top surface. The AgNPs were subsequently deposited on the slide via electrostatic interaction. As a result, a dark yellow film of AgNPs was obtained with maximum absorption at 410 nm. Film fabrication based on LbL assembly provided acceptable reproducibility (relative standard deviation = 6.5%). The fabricated film had long-term stability (>6 weeks). A very small quantity of AgNPs was used in this method. Fabrication was performed under ambient conditions. Therefore this fabrication was considered as a green method. The AgNP modified slide was developed to sense hydrogen peroxide. Detection is based upon oxidation of AgNPs by hydrogen peroxide. This results in a change in colour of the film from dark yellow to colourless. Linear calibration was obtained over the range of 1.0--100.0 mM of hydrogen peroxide. The device was successfully used for measuring hydrogen peroxide in urine.
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    A flow-circulation system incorporating a PVP-BiOBr@rGO assembly for simultaneous degradation and detection of oxytetracycline in fish farm wastewater
    (2025-05-27) ;
    Suknakhin, Nichakarn
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    Sonsaket, Thanamat
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    Teerasong, Wanatchaporn
    ;
    This work focuses on developing a new flow-circulation system for simultaneous detection and degradation of oxytetracycline (OTC) in fish farm wastewater to address a need for antibiotic abatement in wastewater treatment. Polyvinyl pyrrolidone capped bismuth oxybromide assembled with a reduced graphene oxide (PVP-BiOBr@rGO) photocatalyst was solvothermally synthesized and characterized. The prepared photocatalyst exhibited a morphological flower-like structure with a high surface area, 47.59 m<sup>2</sup> g<sup>−1</sup>. Its band gap energy was 2.93 eV. A ternary PVP-BiOBr@rGO composite showed lower charge recombination than its pure form. PVP-BiOBr@rGO was filled inside a catalyst column of a flow system, with a spectrophotometer at the column end. Wastewater was continuously transported through the column and OTC spectrophotometrically examined during its degradation. The wastewater was recirculated until the OTC concentration was minimized. This system achieved 90.3% degradation of OTC within 180 min. The catalyst column could be regenerated for 2 cycles. The proposed flow system offers the advantages of ease of use, inline operation, and real-time sensing. This highlights a potential for real-world sustainable wastewater treatment applications.
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    Preparation of PVP-BiOBr Adsorbent for Efficient Indigo Carmine Dye Removal Using Flow-Circulation Systems
    (2024-07-09) ;
    Saenghirun, Thanakrit
    ;
    Sunthornchainukul, Thanawat
    ;
    Thammaso, Supinya
    ;
    Chompoosor, Apiwat
    This work presents an adsorptive removal of indigo carmine (IC) dye using a polyvinylpyrrolidone capped bismuth oxybromide (PVP-BiOBr) adsorbent. PVP-BiOBr was synthesized via a simple precipitation method. The morphology and surface chemical structure of the adsorbent were characterized using XRD, SEM, FTIR, and BET analyses. The adsorption isotherm and kinetics were investigated to reveal the mechanism of dye removal. Prepared PVP-BiOBr has a crystallite size of 19.7 nm, with a mean particle size of ∼2 μm and a surface area of 5.14 m<sup>2</sup> g<sup>-1</sup>. The optimum pH for this adsorptive process spanned the range of 4 to 9. Experimental data indicated applicability of the Langmuir isotherm model, and the study confirms a pseudo-second-order kinetics model. The maximum adsorption capacity for IC dye was 208.3 mg g<sup>-1</sup>. A flow-circulation system was developed for the treatment of IC dye contaminated water samples. PVP-BiOBr was packed inside a column and did not spill into the water sample after treatment. The removal efficiency was ≥90% after 25 min. The PVP-BiOBr adsorbent could be reused for three cycles. This work demonstrates that PVP-BiOBr is a promising candidate as an adsorbent for IC dye removal. Additionally, the flow-based system establishes an automated operation in continuous mode, which is viable for large scale applications.
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    A low-cost method for determination of calcium carbonate in cement by membraneless vaporization with capacitively coupled contactless conductivity detection
    (2010-05-15)
    Sereenonchai, Kamonthip
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    Chan-Eam, Sumonmarn
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    Saetear, Phoonthawee
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    This work presents a flow analysis method for direct quantitation of calcium carbonate in cement without pretreatment of the sample. The method is based on online vaporization of CO<inf>2</inf> gas following acidification of the sample inside a small chamber that has a flow of acceptor solution passing around it. Solubilization of the CO<inf>2</inf> gas into the acceptor stream changes the conductivity of the acceptor solution causing an increase of signal at the capacitively coupled contactless conductivity detection (C<sup>4</sup>D) placed at the outlet of the vaporization chamber. This chamber is an adaption from previous work reported on 'membraneless vaporization' (MBL-VP). The method can be used in the quality control of production of mixed cement. These cement materials usually have calcium carbonate contents at high concentration range (e.g., 33-99% (w/w) CaCO<inf>3</inf>). Analysis of samples by this method is direct and convenient as it requires no sample pretreatment. The method is low-cost with satisfactory accuracy and acceptable precision. © 2010 Elsevier B.V.
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    A new mechanism for resonance Rayleigh scattering detection of minoxidil based on catalytic oxidation of silver nanoparticles
    (2022-07-05) ; ;
    Chompoosor, Apiwat
    This work presents a new method for minoxidil detection based on silver nanoparticle (AgNP) oxidation. Minoxidil, which is a pyrimidine N-oxide, can be reduced to its corresponding pyrimidine via a redox reaction. In this system, acetate buffer serves as a proton source. AgNPs act as electron donors that contribute electrons to the reaction, producing Ag<sup>+</sup>. Consequently, the sizes and numbers of AgNPs in the system decrease, which results in a decline in their resonance Rayleigh scattering (RRS). By monitoring the RRS intensity at 409 nm, a change in intensity was linearly related to the minoxidil concentration over a concentration range of 0.5 – 5.0 mM. The detection limit was 0.35 mM. This approach is simple and rapid. It is done by directly mixing the drug and AgNPs in an acidic buffer. The reaction was completed within 2 min. This proposed method was successfully utilized for quantification of minoxidil in topical hair-growth formulations.