Teerasong, Saowapak

Preferred name

Teerasong, Saowapak

Alternative Name

Teerasong, S.

Main Affiliation

School of Science

saowapak.te@kmitl.ac.th

Now showing 1 - 4 of 4

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Direct determination of ethanol in alcoholic beverages based on its anti-aggregation of melamine-silver nanoparticle assembly
(2022-12-01)
Duangdeewong, Chomphunud
;
Choengchan, Nathawut
;
Wattanasin, Panwadee
;
Teerasong, Saowapak
We report a new method for determination of ethanol based on anti-aggregation of silver nanoparticles (AgNPs) in the presence of melamine. In the system, ethanol and melamine act as protecting and aggregating agents, respectively. Melamine can induce citrate-stabilized AgNPs to aggregate, leading to a color change from yellow to green. However, if the AgNPs are pre-incubated in ethanol, ethanol readily surrounds the particles by forming hydrogen bonds with the citrate stabilizer. An external nanoshell of ethanol hinders particle aggregation caused by melamine. Minor aggregation of AgNPs was observed, the solution color maintained its yellow-orange color. Higher ethanol concentrations result in a lower degree of particle aggregation. The colorimetric response of AgNPs was monitored using a UV–vis spectrophotometer at 390 nm. The current method could determine ethanol concentrations over a wide dynamic range of 5–80% (v/v), with a detection limit of 3.1% (v/v) (3SD of blank/slope). This method was applied for direct quantification of ethanol in alcoholic drinks without sample pretreatment and the results are well correlated with those of gas chromatography. Our method is convenient and cost effective, making it auspicious for ethanol monitoring in alcoholic drink manufacture and control.
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Smartphone RGB camera-based colorimetric platform with double-layer spherical Ca (II)-alginate/ZnONPs hydrogel liquid-core curcumin emulsion for rapid and selective detection of pyridoxine (Vitamin B6) in functional beverages
(2025-08-01)
Chinnawat, Sirinyakorn
;
Detsri, Ekarat
;
Lerdpiriyaskulkij, Natee
;
Teerasong, Saowapak
;
Mathaweesansurn, Arjnarong
A portable smartphone-based RGB colorimetric sensor was developed for the quantitative detection of pyridoxine (vitamin B6, VB-6) in functional beverages, employing a novel core–shell hydrogel probe (CUR<inf>Hydrogel</inf>), engineered with a calcium alginate matrix encapsulating a liquid-phase curcumin emulsion and externally layered by poly(diallyl dimethyl ammonium chloride)-functionalized ZnO nanoparticles (ZnONPs/PDADMAC). The CUR<inf>Hydrogel</inf> spheres were fabricated via a molecular self-assembly reverse spherification and demonstrated high mechanical stability (stiffness: 6.45 × 104 N/m, compressive strength: 1.01 × 106 N/m2) along with excellent UV-blocking photostability for up to 28 days. The CUR<inf>Hydrogel</inf> probe was applied for detecting VB-6 via a smartphone-based sensing platform. The colorimetric assay was based on a two-step strategy involving initially the formation of the colorless pyridoxine-boron complex by VB-6 and boric acid, after which the remaining boric acid binds with curcumin to produce a red rosocyanine dye. A higher VB-6 concentration yields less rosocyanine and a discernible color shift from orange to yellow, which is quantified via smartphone RGB analysis. The RGB values were assessed via a smartphone application, providing the linearity of VB-6 detection of 10–125 mg L−1 with LOD and LOQ of 2.93 mg L−1 and 9.77 mg L−1. The CUR<inf>Hydrogel</inf> exhibited excellent precision with relative standard deviations (RSDs) ranging from 0.20 to 0.27 %, while recoveries ranged from 99.84 % to 103.03 %. Particularly, the results of this method were also validated by comparing with HPLC and UV–vis spectrophotometry. The smartphone RGB camera-based colorimetric sensor of CUR<inf>Hydrogel</inf> has great potential application prospects for detecting VB-6 in functional beverage samples.
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Eco-friendly pectin/BCG film sensor: a smartphone-compatible platform for gamma-aminobutyric acid detection
(2026-05-01)
Niamlaoong, Rungthiwa
;
Teerasong, Saowapak
;
Phengdaam, Apichat
;
Tantirungrotechai, Yuthana
;
Wattanasin, Panwadee
This work introduces a biodegradable pectin–bromocresol green (BCG) film integrated into a microplate platform for the colorimetric determination of gamma-aminobutyric acid (GABA). The sensing mechanism relies on electrostatic ion-pair formation between the protonated amine group of GABA (-NH₃+) and the sulfonate group of BCG, producing a distinct color transition from yellow to green. The resulting colorimetric response was quantitatively analyzed using a smartphone-assisted RGB system coupled with ImageJ software. Molecular modelling provided insight into the interaction mechanism and indicated that the GABA-BCG association is dominated by electrostatic interactions. Under optimized conditions, the developed assay exhibited a wide linear range (0.1–1000 mg L−1) with detection and quantification limits of 0.07 mg L−1 and 0.22 mg L−1, respectively. Good analytical precision was achieved (%RSD ≤ 5%), while negligible interference from common amino acids, vitamins and food additives was observed. The method was successfully applied to beverage and dietary supplement samples, demonstrating good agreement with HPLC analysis. The biodegradable BCG–pectin film platform provides a rapid, low-cost, and portable approach for GABA determination and represents a promising sensing strategy aligned with the principles of green analytical chemistry.
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A rapid cotton swab for on-site screening of coloring curcumin on durian skin: food safety aspects
(2023-08-01)
Teerasong, Saowapak
;
Boonyaratsewee, Phanaporn
;
Aunruan, Panuwat
;
Saard, Worawan
;
Saetear, Phoonthawee
Exported durians from Thailand are sometimes immersed in curcumin to give the fruits a good appearance. Curcumin is regarded as non-toxic additive, however some importing countries prohibited use of any additive to fresh fruits and vegetables. This work aims to develop a rapid, low cost and convenient cotton swab device for curcumin detection. The detection principle involves a colorimetric acid–base characteristic of curcumin. Curcumin in an acidic/neutral solution presents a bright yellow color, while it displays an intense orange–red color in basic solution. A cotton swab acted for both sample collection and as a sensing platform. A pre-moistened swab was used to wipe a durian surface. Afterward, a NaOH solution was dropped onto the swab. A distinct orange–red color appearing on the swab indicates the presence of curcumin. The cotton swab was applied for qualitative analysis of curcumin contaminated on durian husks via visual detection. The developed device provided good reliability, 93.75% (36 samples). Furthermore, the device was demonstrated for quantitative determination using camera detection. Two linear calibrations were obtained in ranges of 10–75 and 75–250 mg L−1, with a detection limit of 3.2 mg L−1. The method was also successfully applied to quantification of curcumin in durians (three samples) and dietary supplements (two samples). The test can be done in a few minutes. The developed device was established as an useful tool for food safety and control of contamination by curcumin in an on-site application. Graphical abstract: [Figure not available: see fulltext.]

Teerasong, Saowapak

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