Bunyaatichart, Kanokpon

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Bunya-Atichart, Kanokpon

Bunya-atichart, Kanokpon

Bunya-Atichart, K.

Bunya-Atichart, Kanokporn

Bunya-Athichart, Kanokpon

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KMITL Prince of Chumphon Campus

kanokpon.bu@kmitl.ac.th

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Effects of 1-Methylcyclopropene, Gibberellic Acid, and Aloe vera Coating on Lime Storage Life and Fruit Quality
(2023-01-01)
Lerslerwong, Ladawan
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Buapuean, Chompoonut
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Rugkong, Adirek
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Bunya-Atichart, Kanokporn
Limes (Citrus aurantifolia Swingle) have a short postharvest life. Therefore, effective treatments increasing storage life to lengthen the time that produce remains fresh is desirable. This study evaluated the effects of postharvest treatments of 1-methylcyclopropene (1-MCP), gibberellic acid (GA<inf>3</inf>), and Aloe vera on lime storage life and fruit quality. The optimum individual treatments of 50% Aloe vera coating, 300 mL·L−1 GA<inf>3</inf>, and 750 nL·L−1 1-MCP were identified as most effective in extending lime postharvest life. Under ambient conditions (26.4 ± 1.0°C, 64.4 ± 7.4% RH), 1-MCP fumigation was the best treatment, increasing lime postharvest life to 28.2 days. The 1-MCP-treated fruit had a higher juice content than the control fruit. Also, soluble solid contents and titratable acidity were not significantly different, while ascorbic acid was lower than the control. Under cold room conditions (10.0 ± 0.3°C, 68.9 ± 12.4% RH), 1-MCP fumigation followed by Aloe vera coating was the best treatment, increasing lime storage life by 38.7 days. The longer storage time did reduce juice content in treated limes compared to untreated fruit. However, the treatment did not affect the soluble solid contents, titratable acidity, or ascorbic acid level. In conclusion, for optimum postharvest life in limes stored under ambient conditions, fumigation with 1-MCP is best. While for limes stored in cold rooms, a cotreatment of 1-MCP fumigation followed by Aloe vera coating maximizes postharvest life.
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Histological and biochemical aspects as potential markers for evaluation graft compatibility in durian at the early nursery stage
(2024-11-01)
Thippan, Saithip
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Bunya-Atichart, Kanokporn
;
Ayuttaya, Supat Isarangkool Na
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Lerslerwong, Ladawan
Despite being primarily utilized for the commercial propagation of durian seedlings, there still needs to be more understanding regarding the histological and biochemical changes that durian plants undergo during compatibility. Further research is required to elucidate this aspect of durian plant biology. The investigation utilized the cleft-grafted ‘Monthong’ durian cultivar onto indigenous durian rootstock. Examining histological and transverse sections elucidated the three fundamental biological processes involved in graft union formation: scion-rootstock adhesion and wound response, callus bridge construction, and vascular reconnection essential for effective wound healing. These changes are related to the appearance of successfully grafted durian with new sprouting, smooth graft union, and dark green leaves. Quantification of endogenous hormones through LC-MS analysis during the process of graft union formation revealed distinct levels of auxins (IAA), cytokinins (zeatin), gibberellins (GA<inf>3</inf>), and abscisic acid (ABA) at various stages of graft development post-grafting. Low levels of IAA were observed during the wound response, callus cell formation, and callus differentiation. It was accompanied by significantly higher concentrations of zeatin, which were present at all stages, with particularly pronounced levels during callus bridge formation and vascular reconnection. Upon completion of the union formation, the highest levels of IAA, zeatin, and GA<inf>3</inf> were observed simultaneously with the emergence of a new sprout in the graft, coinciding with the whole connection of the new vascular cambium between the scion and rootstock. After the grafting, ABA appeared only three days later, at the time of the wound response. The phenolic compounds in the durian graft union decreased gradually after the vascular cambium of the scion and rootstock had become fully connected, following the callus formation and proliferation period. Total non-structural carbohydrates (TNC) levels at the graft union area peaked during the initial wound response phase and gradually declined as callus bridge formation and wound healing progressed. TNC increased during vascular cambium formation and decreased when the graft produced new sprouts. The anatomical and visual characteristics, endogenous hormones, phenolic acid, and TNC were influential in the modulation of graft union formation and the early growth of the 'Monthong' durian cleft graft on the indigenous rootstock. These parameters show the potential markers detected since the early nursery stage of durian graft.
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Effect of plant growth regulators on in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr.
(2023-02-01)
Montri, N.
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Charoensrimuang, S.
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Deewatthanawong, R.
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Montri, W.
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Bunya-Atichart, K.
The effect of plant growth regulators on the in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr. was evaluated. The protocorms were cultured in VW media supplemented with combinations of indole acetic acid (IAA) and thidiazuron (TDZ) at the concentrations of 0, 0.1 and 0.2 mg L-1. The 2 leaf stage seedlings were cultured on VW media supplemented with cytokinins; 6-furfurylaminopurine (kinetin: K), benzyladenine (BA) and TDZ or auxins; IAA, indole-3-butyric-acid (IBA), α-naphthalene acetic acid (NAA) at the concentrations of 0, 2 and 4 mg L-1 for 2 months. The results found that combinations of IAA and TDZ had higher protocorm fresh weight when compared with control. Adding with 0.1 and 0.2 mg L-1 IAA alone were promoted protocorm fresh weight. On the other hand, combinations of 0.1 mg L-1 IAA and 0.2 mg L-1 TDZ also improved the fresh weight number. The combinations of TDZ and IAA treatments were achieved for the number of 1st stage protocorm. For 2 leaf stage seedling culture, treated with cytokinins was not significantly different in shoot length, seedling fresh weight and leaf number. However, cytokinins suppressed root number compared to untreated. The maximum root numbers at 4.21 roots were found in free cytokinins medium. Seedling fresh weight and leaf numbers were significantly increased when increased the concentration of auxins. Our results indicated that auxins and cytokinins played an instrumental role for P. hookeriana seedling growth.
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Plant host selection for spore production of Glomas mosseae
(2022-04-01)
Deewatthanawong, R.
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Bannajit, W.
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Deewatthanawong, P.
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Bunya-Atichart, K.
Five species including Ipomoea aquatica, Triticum aestivum, Oryza sativa, Zea mays, and Vigna radiata were used as plant hosts for spore production of Glomas mosseae. Seeds of all plant species were surface sterilized and grown in sterilized growing medium with a ratio of 1 part soil to 8 part sand. Each pot of each plant species was inoculated with 30 spores of G. mosseae and compared with the control without inoculum. Each treatment consisted of five replicates containing four pots. Half-strength Hoagland solution was applied once a week and plant growth parameters including diameter, height, and number of leaves were recorded every week for 2 months. Spores of G. mosseae in the rhizosphere, root colonization, and plant dry mass were measured after 2 months of inoculation. Results showed that G. mosseae improved plant growth of all plant species, with maximum increase in plant diameter found in Z. mays; while, maximum effects on plant height and root dry mass were observed in V. radiata. Results of spore formation suggested Z. mays as the most suitable host for mass spore production with the highest spore numbers of 97.4 100 g-1 soil; however, no significant differences in root colonization were shown among the five plant species.
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Application of Melaleuca cajuputi Powell crude extract on controlling postharvest anthracnose disease in Gross Michel banana (Musa (AAA group) ‘Kluai Hom Thong’)
(2026-03-01)
Montri, N.
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Leujantuak, P.
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Tongsri, V.
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Deewatthanawong, R.
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Bunya-Atichart, K.
Exporting Gross Michel bananas, known as ‘Kluai Hom Thong’ in Thailand, to Japan involves several considerations to ensure compliance with Japan’s stringent food safety and quality standards, especially regarding pesticide residues and other contaminants. The research aimed to evaluate the impact of various concentrations of Melaleuca cajuputi Powell crude extract on controlling postharvest anthracnose disease in Gross Michel bananas, focusing on developing a non-toxic production method. The 70% ripening stage bananas were dipped with 0, 800, 1,000, and 1,200 mg L-1 crude extract for 2 min before spraying with 10-6 spore mL-1 of Colletotrichum musae spore suspension, followed by a 2-day incubation, subsequent spraying with 500 mg L-1 of ethephon solution, and a further 1-day incubation at ambient temperature (29±2°C). The shelf-life of banana, weight loss, total soluble solids, and surface morphology of banana peel were analyzed. Results showed that applying M. cajuputi Powell crude extract could extend shelf-life and maintain the quality of banana fruits. The 800 mg L-1 crude extract resulted in 2.13% weight loss, with peel density at 14.80 N cm-3 and pulp density at 6.23 N cm-3. Additionally, it resulted in the lowest percentage of anthracnose disease at 7.5% and the longest shelf life of 8 days.
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Paclobutrazol enhances yield and secondary compound accumulation in Curcuma longa L.
(2024-11-01)
Montri, N.
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Chauytam, S.
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Deewatthanawong, R.
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Bunya-Atichart, K.
The results indicated that the application of different concentrations of PBZ at 60 days preharvest had an impact on yield after harvest at 9 months while the color of turmeric remained relatively consistent. In all treatments, the rhizome color was yellow to orange. The fresh weight of the rhizome showed no significant differences within the range of 0-800 mg/L PBZ treatments, with the lowest yield observed in 1,200 mg/L PBZ treatment. The dry weight percentage was highest when treated with 800 mg/L PBZ for the mother rhizome and 600 mg/L PBZ for the finger rhizome. Regarding the accumulation of secondary compounds, highly significant differences among the treatments were found for the content of total flavonoids and total phenolics while curcumin showed significant differences. The treatment with 1,200 mg/L PBZ had the highest total phenolics content at 78.18 mg GAE/gDW and total flavonoids at 295.54 mg QUE/gDW. PBZ-treated plants also showed higher curcumin content and percentage than the non-treated plants. These findings indicated that the application of PBZ has the potential to improve both the yield and quality of turmeric, which is advantageous for cultivating this valuable medicinal plant.
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Application of calcium chloride on yield and quality of Capsicum annuum L.
(2024-09-01)
Montri, N.
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Sengseng, S.
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Deewatthanawong, R.
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Bunya-Atichart, K.
Application of calcium chloride on yield and quality of Capsicum annuum L. 'Super Hot' were investigated. The experiments were conducted in randomized complete block design (RCBD). 0, 20, 40, 60, 80, and 100 mg L-1calcium chloride was sprayed preharvest at the orange-red ripening stage. The ripe red fruits were harvested. Yield and quality in terms of fruit color and secondary compound content were measured. The results showed that spraying with various concentrations of calcium chloride did not affect the percentage of dry weight and color of fruit but significantly influenced fresh and dry weight, capsaicin, total phenolics, and total flavonoid contents. Preharvest spraying of calcium chloride at 40 mg L-1treatment gave the maximum fresh and dry weight and total phenolics at 1,257.83 g, 361.35 g, and 44.75 mg GAE g-1DW, respectively. A high content of total flavonoid at 63.50 and 6.75 mg QE g-1DW was observed in 20 to 40 mg L-1treatment, while high contents of capsaicin contents at 64.32 mg g-1 DW were achieved in 80 mg L-1,.

Bunyaatichart, Kanokpon

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