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    Item type:Publication,
    A "dual-acceptor channel" membraneless gas-diffusion unit for simultaneous determination of ethanol and acetaldehyde in liquors using reverse flow injection
    (2018-01-01) ;
    Poontong, Bangerdsuk
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    ; ;
    Motomizu, Shoji
    A new design of membraneless gas-diffusion unit with dual acceptor channels for separation, collection and simultaneous determination of two volatile analytes in liquid sample is presented. The unit is comprised of three parallel channels in a closed module. A sample is aspirated into the central channel and two kinds of reagents are introduced into the other two channels. Two analytes are isolated from the sample matrix by diffusion into head-space and absorbed into the specific reagents. Non-absorbed vapor is released by opening the programmable controlled lid. The unit was applied to liquors for measurement of ethanol and acetaldehyde using reverse flow injection. Dichromate and nitroprusside were exploited as reagents for colorimetric detection of ethanol and acetaldehyde, respectively. Good linearity ranges (r2 > 0.99) with high precision (RSD < 2%) and high accuracy (recovery: 90 - 105%) were achieved. The results were compared to the results by GC-FID and no significant difference was observed by paired t-test (95% confidence).
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    Item type:Publication,
    A mobile phone-based analyzer for quantitative determination of urinary albumin using self-calibration approach
    This work demonstrates use of a smart mobile phone installed with an Android application, termed ‘Albumin smart test’, as an analyzer for quantitative determination of urinary albumin. The reaction between albumin and tetrabromophenolphthalein ethyl ester (TBPE) in the presence of Triton X-100 was employed for detection principle.The mobile phone was exploited with the sample cassette and the test paper. One sample cassette composes of two holders for accommodation of control and test samples. The test paper was designed in order to contain standard colorimetric strip and space for situating the sample cassette. Optical images of the strip and the samples were simultaneously captured in a single shot by a digital camera of the mobile phone and were digitally processed by the developed application for quantification of the albumin concentration based on self-calibration approach. With the advantage of self-calibration, the albumin test by our mobile phone can be performed in ambient light without using any extra module integrated with lighting control device. The other advantages are portability, ease of implementation and rapid analysis (3 min) with high precision (RSD ≤ 2.5%) and high accuracy (Recovery = 98.7% ± 1.6). The mobile device was successfully applied to diagnosis of microalbuminuria.
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    Item type:Publication,
    A design of colorimeter for chemical quantitative analysis based on a web-camera
    (2017-10-19)
    Phongern, Komsun
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    ; ;
    Chengchan, Nathawut
    This paper presents the design of colorimeter for chemical quantitative analysis based on image processing. It comprises of two main parts which are test box and software. The test box consists of a web camera and LED lamp plat which is used to illuminate a micro wells. The software is graphic user interface and image processing. The main of image processing are split RGB channel of each pit in micro wells so calculate calibration curves for finding and unknown concentric solution then show result on screen. The experiment shows the design in this paper can calculate concentric of the solution. The result of the experiment have a correlation of reaction between Fe (II) and Phenanthroline (Red product) 0.985, the reaction between Ethanol and Methyl orange (Yellow product) 0.933 and reaction between Uric acid and Phosphotungstic acid (Blue product) 0.986. Moreover it takes a less time and lower cast compared with the spectrophotometer. The device can be applied to chemical laboratory by possibility.