Laosinwattana, Chamroon
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Preferred name
Laosinwattana, Chamroon
Alternative Name
Laosinwattana, C.
Main Affiliation
Email
chamroon.la@kmitl.ac.th
27 results
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Item type:Publication, Potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus leaf extract in minced pork(2017-09-01) ;Nguyen, Tuyen Thi Kim; ; Objective: This study investigated the effect of extraction solvents on antioxidant bio-active compounds as well as potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus (P. acidus) leaf extract in minced pork. Methods: The effect of various solvent systems of water, 25%, 50%, 75% (v/v) ethanol in water and absolute ethanol on the extraction crude yield, total phenolic content, total flavonoid content and in vitro antioxidant activities of P. acidus leaves was determined. In addition, antioxidant activities of the addition of crude extract from P. aciuds leaves at 2.5 and 5 g/kg in minced pork on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation decolorization, reducing power and inhibition of lipid peroxidation (thiobarbituric acid reactive substances; TBARS) were determined. Moreover, sensory evaluation of the samples was undertaken by using a 7-point hedonic scale. Results: The results showed that the highest crude yield (2.8 g/100 g dry weight) was obtained from water which also had the highest recovery yield for total phenolic content, total flavonoid content and the strongest antioxidant activity. The addition of crude water extract from P. acidus leaves was more effective in retarding lipid peroxidation and higher antioxidant activity than control and butylated hydroxytoluene in minced pork. In particular, the samples containing P. acidus extract had no significant effect on the sensory scores of overall appearance, color, odor, texture, flavor, and overall acceptability compared to the control. Conclusion: Water solvent was an optimally appropriate solvent for P. acidus leaf extraction because of its ability to yield the highest amount of bio-active compounds and in vitro antioxidant property. Particularly, P. acidus crude water extract also strongly expressed the capacity to retard lipid oxidation, radical scavenging, radical cation decolorization and reducing power in minced pork. The results of this study indicated that P. acidus leaf extract could be used as natural antioxidant in the pork industry. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)(2015-11-01) ;Thanaboripat, Dusanee; ; ; An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of natural herbicide from tagetes erecta on echinochloa crus-galli (L.) beauv(2018-01-01) ;Wichittrakarn, P.; ; We studied the inhibitory effects of leaves extracts of Tagetes erecta on weed-seed germination and the mechanisms involved. Hydroethanolic crude extract (OR) from Tagetes erecta dried leaves was partitioned into four fractions: hydrolyzed (HY), aqueous (AQ), neutral (NE) and acidic (AE). The inhibitory effects of crude extracts and its fractions were evaluated on germination and seedlings growth of Echinochloa crus-galli (L.) Beauv. The total phenolic and flavonoid contents of each fraction were also determined. Germination and seedling growth were drastically inhibited by HY and AE followed by NE, OR and AQ. The HY and AE fractions had higher concentrations of both phenolics and flavonoids than other fractions. The HY fraction was selected as most potent for development as natural herbicide due to its high yield and strong inhibitory effects. The HY fraction was formulated into a soluble concentrate product (SCT) and its inhibition potential and modes of action were investigated. The SCT drastically inhibited the seed germination of E. crus-galli, inhibiting both imbibition, α-amylase activity and also seedling growth. Cytogenetic bioassays of SCT on root-tip cells of Allium cepa L. showed that it inhibited the mitosis and thereby the cell division. It is likely that a natural herbicide may be developed from the hydrolyzed fraction of a hydroethanolic extract of Mexican marigold leaves. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Potential allelopathic effects of Suregada multiflorum and the influence of soil type on its residue's efficacy(2010-09-01); ;Boonleom, Chaninat; ;Thitavasanta, SompopThis experiment was conducted to determine the potential allelopathic effects of the aqueous extracts and dried leaf powder of Suregada multiflorum and to find the herbicidal effects of its granule on the weed species, barnyardgrass (Echinochloa crus-galli) and slender amaranth (Amaranthus viridis), in various growth media (germination paper, sand, and soil). Observations in this study demonstrated that the potential and magnitude of their inhibitory effects varied among the plant parts. The leaves and branches possessed the strongest inhibition levels, followed by the bark. The aqueous extract from the leaves of S. multiflorum had a remarkably stronger inhibitory effect on the germination and growth of slender amaranth, compared with barnyardgrass; when applied at the concentration of 100-g-L<sup>-1</sup>, it completely inhibited the germination and initial seedling growth of slender amaranth. In addition, all the treatments of the dried leaf powder had stronger inhibition effects than the aqueous extract. Interestingly, the inhibitory effects of the dried leaf granule of S. multiflorum were stronger than the effects of the dried leaf powder at equal rates. The degree of inhibitory effect of the S. multiflorum granule, applied in various growing media, can be classified in order of decreasing inhibition as germination paper = sand > clay soil. The present study indicates that the S. multiflorum granule product that was developed in this research has the potential to provide a powerful organic herbicide for controlling slender amaranth. © 2010 The Authors. Journal compilation © 2010 Weed Science Society of Japan. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Chemical composition and herbicidal action of essential oil from Tagetes erecta L. leaves(2018-12-15); ;Wichittrakarn, PattharinPlant derived substances, particularly essential oils have been an increasing interest in their safe and environmentally-friendly application to crops, as a powerful alternative to chemical herbicides. The present investigation studied the chemical composition of the essential oil extracted from Tagetes erecta L. and evaluated its herbicidal activities (pre-and post-emergence) against Echinochloa cruss-galli (L.) Beauv. The yield of essential oil from T. erecta was 0.72 mg.kg<sup>−1</sup> fresh weight. Gas chromatography-mass spectrometry was employed to determine essential oil chemical composition. Relatively high amounts of monoterpines were detected, consisting mainly of piperitone (17.12%), piperitenone (10.46), and ocimine (8.59%); identified sesquiterpenoids consisted mainly of neophytadiene (16.18%) and caryophyllene (11.10%). The essential oil was formulated as emulsifiable concentrate (EC-EO) for herbicidal applications. Seed germination and seedling growth of E. crus-galli was determined by Petri dish bioassay for EC-EO concentrations ranging from 0.25 to 2 mL.L<sup>−1</sup>. At 2 mL.L<sup>−1</sup>, the EC-EO completely inhibited germination and growth of E. crus-galli seed due to the inhibition of α-amylase activity. Post-emergence application of EC-EOs was also tested, with concentrations of 10 to 80 mL.L<sup>−1</sup> foliar applied to 21-day-old E. crus-galli plants. The leaves of treated plants appeared wilted and desiccated. Increasing concentrations of oil decreased chlorophyll a, b, and carotenoid content, indicating that the EC-EO interferes with photosynthetic metabolism. The EC-EOs also caused loss of membrane integrity and increase of thiobarbituric acid reactive substances. Taken together, these results indicate that the T. erecta essential oil has phytotoxic compounds that could be utilized as natural herbicides for future weed control. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Essential oil from ginger as a novel agent in delaying senescence of cut fronds of the fern (Davallia solida (G. Forst.) Sw.)(2019-10-01); Rabbit's foot fern (Davallia solida (G. Forst.) Sw.) is traded as cut floral greens and commonly used in orchid flower arrangements and bouquets; however, its use is limited by its relatively rapid postharvest yellowing and desiccation. This study was designed to investigate the chemical profile of the essential oil from ginger rhizome (Zingiber officinale Roscoe) and to determine the oil's effectiveness in extending the postharvest quality of rabbit's foot fern. Essential oil was extracted from ginger rhizomes by hydrodistillation. The percentage composition of major identified compounds was citral (29.91%), zingiberene (13.3%), α-farnesene (11.68%), 1,8-cineole (10.60%), and β-sesquiphellandrene (6.14%). The ginger essential oil was applied in emulsion form to rabbit's foot fern fronds as a pulse (25, 50, or 100 mg L<sup>−1</sup>) or a holding solution (5, 10 and 20 mg L<sup>−1</sup>). Pulse treatments were applied for 6 h before the fronds were transferred into a holding solution containing either distilled water or essential oil of ginger. Control fronds received distilled water as both pulsing and holding solutions. Rabbit's foot fern pulsed with 25 mg L<sup>−1</sup> essential oil and transferred into a holding solution with 5 mg L<sup>−1</sup> essential oil increased frond longevity by 4.9 days and helped maintain both water uptake and visual quality. Under this treatment, fronds exhibited higher chlorophyll a and b contents, lower malondialdehyde content, and delayed loss of radical scavenging ability compared to control. Together, these results indicate that when dosed appropriately in pulsing and holding solutions, essential oil of ginger prolongs the vase life of fronds; it also enhances postharvest quality by simultaneously retarding the degradation of chlorophyll a and b, preserving DPPH scavenging activity, and lowering malondialdehyde accumulation. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Micromonospora oryzae sp. Nov., isolated from roots of upland rice(2015-11-01); ;Thanaboripat, Dusanee; ; An actinomycete strain, designated CP2R9-1<sup>T</sup>, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1<sup>T</sup> was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>) and MK-10(H<inf>4</inf>). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1<sup>T</sup> was closely related to Micromonospora haikouensis 232617<sup>T</sup> (99.32% similarity), Micromonospora carbonacea DSM 43168<sup>T</sup> (99.18%) and Micromonospora krabiensis MA-2<sup>T</sup> (99.16%). Strain CP2R9-1<sup>T</sup> was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3¡0.1– 41.7¡0.7%) and phenotypic differences. The results presented in this study showed that strain CP2R9-1<sup>T</sup> represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1<sup>T</sup> (5BCC 67266T5NBRC 110007<sup>T</sup>). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A Potent Phytotoxic Substance in Aglaia odorata Lour.(2016-05-01) ;Kato-Noguchi, Hisashi ;Suzuki, Masahiko ;Noguchi, Kazutaka ;Ohno, OsamuSuenaga, KiyotakeAglaia odorata Lour. (Meliaceae) was found to have very strong allelopathic activity and a bioherbicide PORGANIC<sup>™</sup> was developed from its leaf extracts. However, the phytotoxic substances causing the strong allelopathic activity of the plants have not yet been determined. Therefore, we investigated allelopathic properties and phytotoxic substances in A. odorata. Aqueous EtOH extracts of A. odorata leaves inhibited root and shoot growth of garden cress (Lepidum sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), ryegrass (Lolium multiflorum), and Echinochloa crus-galli with the extract concentration-dependent manner. The extracts were then purified and a major phytotoxic substance with allelopathic activity was isolated and identified by spectral data as rocaglaol. Rocaglaol inhibited the growth of garden cress and E. crus-galli at concentrations > 0.3 and 0.03 μm, respectively. The concentrations required for 50% inhibition ranged from 0.09 to 2.5 μm. The inhibitory activity of rocaglaol on the weed species, E. crus-galli, was much greater than that of abscisic acid. These results suggest that rocaglaol may be a major contributor to the allelopathic effect of A. odorata and bioherbicide PORGANIC<sup>™</sup>. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Inhibitory effects of a variety of aldehydes on Amaranthus tricolor l. And echinochloa crus-galli (L.) beauv.(2018-01-01); ; Thirty-seven commercial aldehydes containing aliphatic chains and aromatic rings as well as heteroaromatic rings were evaluated for their inhibitory activities against Chinese amaranth (Amaranthus tricolor L.) and barnyardgrass (Echinochloa crus-galli (L.) Beauv). Polysorbate 80 (Tween<sup>fi</sup> 80) was used as a surfactant and the research was preliminarily conducted at 400 µM of all aldehydes. Among these aldehydes, (E)-cinnamaldehyde (7) showed the greatest inhibitory effect on seed germination, shoot and root growth of Chinese amaranth by 54.55%, 75.53%, and 85.13% respectively. Similarly, (E)-crotonaldehyde (5), a related α,β-unsaturated aldehyde, inhibited the germination and seedling growth of the tested species at a high percentage. Apart from these two unsaturated aldehydes, no other aliphatic aldehydes had a harmful effect on Chinese amaranth. In terms of benzaldehyde (6), it had no effect on the tested plant; however, many of its derivatives displayed some inhibitory activity. Furthermore, for the ten common heteroaromatic aldehydes, picolinaldehyde (32) had a high inhibitory effect on Chinese amaranth which closely related to the effect of (E)-crotonaldehyde (5) and (E)-cinnamaldehyde (7), whereas, other heteroaromatic aldehydes showed lower effects. In the case of a monocot plant, barnyardgrass, no tested aldehydes reduced seed germination, however, (E)-cinnamaldehyde (7), 2,4,6-trimethoxybenzaldehyde (16) and 4-(dimethylamino)benzaldehyde (24) could inhibit the seedling growth of the plant with low to moderate levels. The herbicidal effects of the most active aldehydes were then further investigated in order to find the minimum concentration of these aldehydes suppressing the germination and growth of the tested plants. At concentrations as low as 50–100 µM some aldehydes could inhibit the seedling growth of the tested species. The structure-activity relationship (SAR) study reported here demonstrates the chemical clues governing the inhibitory activity of aldehydes which could be utilized in the development of highly effective herbicides in the near future. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Retarding Changes of Postharvest Qualities of Sweet Basil (Ocimum basilicum Linn.) by Vapor-Phase Vinegar(2017-10-02) ;Changsawake, Kanokporn ;Krusong, Warawut; Fresh leaves of sweet basil (Ocimum basilicum) were exposed to ambient upland rice vinegar (URV) vapor containing 4% acetic acid (AA) for 0, 2, 4, 6, 8, or 10 min, before packing in polyethylene bags and storing at 12°C. This extended the postharvest storage life of sweet basil by retaining greenness, freshness, and antioxidants. The gas chromatography-mass spectrometry (GC-MS) results showed a number of other volatiles in URV vapor, including ethyl acetate, propane, pentanal, and acetic acid.
