Tongsri, Pajongjit
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Tongsri, Pajongjit
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pajongjit.to@kmitl.ac.th
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Item type:Publication, Enhancing biomass, hydrocarbon and biodiesel properties of green microalga Botryococcus braunii KMITL through gamma and UV radiation exposure(2024-12-01) ;Jongput, Buppha ;Chiwpreecha, Pattanasak; This study investigated the effects of gamma (<sup>137</sup>Cs, 0–250 Gy) and UV (UV-C, 0–12 h) radiation on growth and biodiesel properties of Botryococcus braunii KMITL. For gamma radiation, maximum biomass (1.37 ± 0.02 g L<sup>−1</sup>) was achieved with 50 Gy, while a dose of 200 Gy resulted in the highest hydrocarbon content (51.84 ± 0.20%) and yield (0.66 ± 0.01 g L<sup>−1</sup>). For UV radiation, a 9 h exposure produced the highest biomass (2.45 ± 0.05 g L<sup>−1</sup>), hydrocarbon content (55.01 ± 1.22%), and yield (1.35 ± 0.04 g L<sup>−1</sup>). Algae exposed to gamma radiation within the range of 0–150 Gy exhibited C16:0 as the dominant fatty acid methyl ester (FAME), similar to those exposed to UV radiation, while algae exposed to 200–250 Gy displayed C18:1n9t as the dominant FAME. High levels of gamma and UV radiation were observed to lengthen fatty acid chains and increase unsaturated fatty acids. The cetane values of biodiesel from algae exposed to gamma and UV radiation ranged from 64.55 ± 0.14–66.47 ± 0.20 and 59.43 ± 0.04–65.27 ± 0.22, respectively, all meeting standard criteria. Both gamma and UV radiation also improved the saponification value and cold flow properties of the biodiesel. These findings suggest that controlled levels of gamma and UV radiation effectively enhance hydrocarbon yields with significant implications for biofuel production. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Siamese neem tree as a natural preservative: Chemical profile, antioxidant properties, and antibacterial efficacy against foodborne pathogens and spoilage bacteria(2025-03-01); ;Manichart, Nutcha; ; The objective of the present study was to investigate the effect of various ethanol in water solvent systems (0, 25, 50, 75 and 100 % v/v) on the extraction crude yield, total phenolic content, total flavonoid content, and chemical profile of Azadirachta indica (Siamese neem tree) leaf extracts. In addition, the antioxidant and antimicrobial properties were investigated using in vitro assays. Crude yield was highest for the water extract (5.78 g/100 g DW) and decreased with increased ethanol. Total phenolic and flavonoid contents were highest in the 75 % ethanol extract (26.95 mg GAE/g crude and 23.73 mg QE/g crude, respectively). GC-MS analysis of the 75 % ethanol extract revealed 33 bioactive compounds, accounting for 96.33 % of the total area. Significant components included benzene, 1,4-bis(phenylmethyl)- (15.05 %), and naphthalene, 1,6-dimethyl- (10.98 %); also present were sterols, fatty acids, hydrocarbons, aromatic, and heterocyclic compounds, indicating a complex chemical profile. In antioxidant assays, the 75 % ethanol extract revealed the highest DPPH radical scavenging activity (IC<inf>50</inf>: 143.03 mg/L), reducing power (IC<inf>50</inf>: 378.69 mg/L), and lipid peroxidation (IC<inf>50</inf>: 526.25 mg/L). However, the absolute ethanol extract exhibited both the highest metal chelating activity (IC<inf>50</inf>: 382.60 mg/L) and the strongest antimicrobial activity, inhibiting all 11 tested foodborne pathogenic and spoilage bacteria. The lowest minimum inhibitory concentration (MIC) of 25 mg/mL was observed for Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas fluorescens, Latilactobacillus sakei, Streptococcus sp., and Lactococcus cremoris, with inhibition zone diameters of 27.21, 28.79, 28.19, 17.60, 15.09 and 11.20 mm, respectively. Similarly, the lowest minimum bactericidal concentration (MBC) of 25 mg/mL was obtained for S. aureus and L. sakei, indicating potent antibacterial effects against these species. Future studies should include direct comparative analyses of neem extracts and synthetic preservatives to evaluate further their relative efficacy and applicability in real-world food systems. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cultivation of Wolffia globosa and its application in functional food development(2025-12-01); ; ;Jongput, Buppha; This study assessed the biomass productivity, biochemical composition, and functional food potential of Wolffia globosa cultivated in four nutrient media: AB hydroponic solution, Chlorella medium, 16-16-16 fertilizer, and 16-16-16 supplemented with a vitamin B complex and FeSO<inf>4</inf> (16-16-16-B). Cultivation was conducted for eight weeks, with biomass harvested weekly. Among the treatments, the Chlorella medium produced the greatest biomass yield (133.82–236.00 g FW tank⁻<sup>1</sup>) and daily productivity (23.90–42.14 g FW m⁻<sup>2</sup> day⁻<sup>1</sup>), significantly surpassing the other media (p < 0.05), followed by 16-16-16-B. Medium replenishment at four weeks initially enhanced growth but was later accompanied by a decline in biomass. Biochemical analysis indicated that the Chlorella medium yielded the highest protein content (46.10 ± 0.93% DW), while the AB medium supported the greatest chlorophyll-a (4.08 ± 0.00% DW) and carbohydrate levels (37.21 ± 1.12% DW); all differences among treatments were statistically significant (p < 0.05). Considering cost-effectiveness and nutritional value, the 16-16-16-B medium was optimal for cultivating W. globosa for food applications. Dried biomass from this medium was incorporated into fresh pasta and fried sweet potato balls, significantly enhancing chlorophyll-a, carotenoids, protein, fiber, and calcium contents. Sensory evaluation using a 9-point hedonic scale showed strong consumer acceptance even at higher inclusion levels. These findings highlight the role of nutrient-enriched media, particularly 16-16-16-B, in enhancing biomass yield and nutritional quality of W. globosa, affirming its potential as a functional food ingredient. Beyond nutritional enhancement, W. globosa-based foods exhibit immunomodulatory and anticancer potential through the antioxidant activity of pigments and other bioactive compounds.
