Maneeruttanarungroj, Cherdsak

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School of Science

cherdsak.ma@kmitl.ac.th

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Effect of pH on Biohydrogen Production in Green Alga Tetraspora sp. CU2551
(2017-01-01)
Maneeruttanarungroj, Cherdsak
;
Phunpruch, Saranya
The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in a high H<inf>2</inf> production yield, we further studied the enhancement of the H<inf>2</inf> production capacity by a substrate-level control to the key enzyme, Hydrogenase. This enzyme catalyzes the formation of biohydrogen gas from protons and electrons. Here, we investigated the effect of pH on the production rate which directly affected the flow of proton flux to the hydrogenase enzyme. The result revealed that pH of culture TAP medium was simply controlled by the use of HCl/NaOH solution over the pH range of 6.5 - 9.0 without any effect on the growth of algal cells. The H<inf>2</inf> production was observed in all ranges of our focusing pH with comparable to the control condition (pH 7.0 of TAP medium). In our best condition, an increase of the H<inf>2</inf> yield about 24% was found in cells cultivated in TAP medium adjusted the pH to 6.5 compared to the control condition. Our finding confirms the successful biohydrogen production enhancement by a substrate-level control for the key hydrogenase enzyme.
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Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp. CU2551 grown under anaerobic condition
(2018-04-01)
Maswanna, Thanaporn
;
Phunpruch, Saranya
;
Lindblad, Peter
;
Maneeruttanarungroj, Cherdsak
The green alga Tetraspora sp. CU2551 has previously been identified and characterized as a photosynthetic microorganism with high potential for H<inf>2</inf> production. In the present study, cells of Tetraspora CU2551 were entrapped and immobilized in an alginate matrix with the aim to analyze the effect of cell stacking and a reduced exposure of O<inf>2</inf> to the cells. The results showed that the most favorable immobilization conditions were 4% (w/v) of final alginate concentration and a cell concentration of 0.125 mg cell dry wt/mL alginate with a bead diameter of 2.80–3.35 mm. The H<inf>2</inf> production yields increased when the immobilized cells were incubated in S-deprived medium and this could be repeated at least for 3 production times. Maximal total H<inf>2</inf> production reached 7.68 ± 0.88 mL H<inf>2</inf>/25 mL medium, corresponding to a rate of 1182.45 ± 24.40 nmol H<inf>2</inf>/h/mg DW. This production is about 6 times higher compared to by cells in suspension, 2–10 times higher when compared to by other green algae, and 10–50 times higher when comparing with cyanobacteria. Based on our observations, immobilized cells of Tetraspora CU2551 is considered a very promising biological system for significant photobiological H<inf>2</inf> production by a photosynthetic microorganism.
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Rapid method for DNA isolation from a tough cell wall green alga Tetraspora sp. CU2551
(2016-06-01)
Maneeruttanarungroj, Cherdsak
;
Incharoensakdi, Aran
Genetic studies are important to understand the complex biological system of various organisms. Some eukaryotic green organisms have tough cell wall which precludes the efficient extraction of the genetic materials. Here, we developed the method for simple and rapid isolation of high quality DNA from a green alga Tetraspora sp. CU2551. The cell homogenization procedures were combined with physical force plus heat treatment to disrupt the cell envelope of Tetraspora sp. CU2551. Without protease treatment, vortexing with glass bead for 30–105 s at 70 °C led to the isolation of a high purity DNA which was suitable for downstream process. The improved method was successfully developed and could be applied for the rapid isolation of DNA from other unicellular and filamentous green microalgal strains.
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Bioethanol fermentation from acid/base-treated water hyacinth biomass using fermentation yeasts saccharomyces cerevisiae yrk 017 and candida shehatae ATCC 22984
(2019-01-01)
Ochaikul, Duangjai
;
Jongmeesuk, Atcharaporn
;
Maneeruttanarungroj, Cherdsak
Water hyacinth is considered as one of most abundant lignocellulosic feedstock in Thailand. It has been selected as a substrate for producing ethanol at an economically feasible manner. This study focuses on the bioethanol production from enzyme-treated hydrolysate of acid/base pretreatment water hyacinth. Two different pretreatment methods of water hyacinth were used including either 2 %(v/v) sulfuric acid or 2 %(w/v) sodium hydroxide at 121°C for 15 min. After acid/base pretreatments, the biomass residues in order to release glucose molecules were treated with a commercial cellulase enzyme complex (ACCELLERASE® 1500) at a concentration of 0.30 mL enzyme/g biomass residue. Two yeast species consisting of Saccharomyces cerevisiae YRK 017 and Candida shehatae ATCC 22984 were separately used as inoculum in fermentation process of the hydrolysate from enzymatic treatments. The results showed that ethanol yield obtained from acid pretreatment was slightly higher than the yield from base pretreatment. In acid pretreatment, S. cerevisiae YRK 017 could produce bioethanol in a yield of 3.82 ± 0.10 g/L, whereas C. shehatae ATCC 22984 could produce yielding 2.85 ± 0.04 g/L.
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Comparison of conventional polymerase chain reaction and routine blood smear for the detection of Babesia canis, Hepatozoon canis, Ehrlichia canis, and anaplasma platys in Buriram Province, Thailand
(2019-01-01)
Rucksaken, Rucksak
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Maneeruttanarungroj, Cherdsak
;
Maswanna, Thanaporn
;
Sussadee, Metita
;
Kanbutra, Pithai
Background and Aim: Dog blood parasites are important tick-borne diseases causing morbidity and mortality in dogs worldwide. Four dog blood parasites species are commonly found in Thailand: Babesia canis, Hepatozoon canis, Ehrlichia canis, and Anaplasma platys. They are transmitted easily by tick species. However, there is little prevalence data available in Thailand. Diseases presentation of blood parasites infection is similar, but the treatment of each species is different. Current diagnosis mainly relies on microscopic examination of a stained blood smear, which has low sensitivity. Therefore, accurate diagnosis is important. This study aims to evaluate the efficacy of the conventional polymerase chain reaction (PCR) method and routine blood smears in the detection of four blood parasites species in dogs from Buriram Province, Thailand. Materials and Methods: In total, 49 EDTA-blood samples were collected from dogs in Buriram Province, Thailand. Blood parasite infection was compared using the Giemsa-stained blood smear technique to identify the parasite under a 100× oil immersion with PCR amplification of the 18S rDNA gene of B. canis and H. canis and the 16S rDNA gene of E. canis and A. platys. Results: Only one dog out of 49 was positive for H. canis based on microscopic examination whereas the PCR results showed that 2.04% (1/49), 4.08% (2/49), 36.73% (18/49), and 30.61% (15/49) of dogs were positive for B. canis, H. canis, E. canis, and A. platys, respectively. Moreover, coinfection was found in 16.33% (8/49) of dogs. Conclusion: This study is the first report to demonstrate the molecular prevalence of blood parasites in domestic dogs in Buriram Province. The results indicated that the PCR method exhibited much higher sensitivity and reliability for blood parasites diagnosis in dogs. Therefore, our data support serious concern regarding the diagnostic technique used in routine blood testing and also provide prevalence data for the management and control of blood parasites in this area.

Maneeruttanarungroj, Cherdsak

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