Thawai, Chitti

Preferred name

Thawai, Chitti

Main Affiliation

School of Science

chitti.th@kmitl.ac.th

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Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)
(2015-11-01)
Thanaboripat, Dusanee
;
Thawai, Chitti
;
Kittiwongwattana, Chokchai
;
Laosinwattana, Chamroon
;
Koohakan, Prommart
An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T).
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Asanoa endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Boesenbergia rotunda
(2016-03-01)
Niemhom, Nantawan
;
Chutrakul, Chanikul
;
Suriyachadkun, Chanwit
;
Thawai, Chitti
A novel Gram-stain-positive, non-motile, endophytic actinomycete, designated strain BR3-1T, which produced spore chains borne on the tips of short sporophores, was isolated from the rhizome of Boesenbergia rotunda collected from Udon Thani province, Thailand. This strain was investigated for its taxonomic position using a polyphasic approach. The strain contained 3-hydroxydiaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars comprised glucose, mannose, rhamnose, ribose and xylose. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides were found as the characteristic phospholipids. The predominant menaquinones were MK-10(H<inf>8</inf>) and MK-10(H<inf>6</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf> and anteiso-C<inf>15:0</inf>. The G+C content of the genomic DNA was 71.4 mol%. 16S rRNA gene sequence analysis revealed that strain BR3-1T belonged to the genus Asanoa and was most closely related to Asanoa ishikariensis (99.39%), Asanoa iriomotensis (99.31%), Asanoa siamensis (99.17%), Asanoa ferruginea (98.84%) and Asanoa hainanensis (98.71%). The DNA–DNA relatedness value between strain BR3-1Tand its phylogenetically closest relatives was in the range of 15.4%±1.2 to 45.8%±2.6. In addition, some physiological and biochemical properties indicated that strain BR3-1T could be readily distinguished from all type strains in the genus Asanoa. Thus, strain BR3-1T should be classified as a representative of a novel species, for which the name Asanoa endophytica sp. nov. is proposed. The type strain is BR3-1T(5BCC 66355T=NBRC 110002T).
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Jishengella zingiberis sp. Nov., isolated from root tissue of zingiber montanum
(2018-10-01)
Thawai, Chitti
;
He, Ya Wen
;
Tadtong, Sarin
A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose. The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol. The major menaquinone was MK-9 (H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>15: 0</inf> and C<inf>17: 1</inf> w8c. The G+C content of the genomic DNA was 70.6 mol%. 16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %). Based on the data of physiological and biochemical tests, including the result of DNA–DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengella zingiberis sp. nov. is proposed. The type strain is PLAI 1-1T (=TBRC 7644T =NBRC 113144T ).
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Dactylosporangium maewongense sp. nov., isolated from soil
(2010-05-01)
Chiaraphongphon, Suthamat
;
Suriyachadkun, Chanwit
;
Tamura, Tomohiko
;
Thawai, Chitti
Morphological and chemotaxonomic characterization of actinomycete strain MW2-25T, isolated from tropical forest soil in Nakhon Sawan Province, Thailand, clearly demonstrated that this strain belongs to the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that this strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium aurantiacum IFO 12592T (99.3% sequence similarity). DNA-DNA hybridization values and some physiological and biochemical properties indicated that this strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Dactylosporangium maewongense sp. nov. is proposed. The type strain is MW2-25T (=BCC 34832T=JCM 15933T). © 2010 IUMS.
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Identification and antimicrobial activity of Micromonospora strains from Thai peat swamp forest soils
(2018-04-01)
Thawai, Chitti
;
Kanchanasin, Pawina
;
Ohkuma, Moriya
;
Kudo, Takuji
;
Tanasupawat, Somboon
The identification and antimicrobial activity of thirteen actinomycete strains isolated from peat swamp forest soils collected from Narathiwat, Patthaloong and Yala provinces, the southern part of Thailand were carried out. Based on the phenotypic and chemotaxonomic characteristics, all isolates were belonged to genus Micromonospora. They were separated into six groups based on 16S ribosomal RNA gene sequence analysis and were identified as M. narathiwatensis (Group 1, 5 isolates), M. humi (Group 4, 3 isolates), M. aurantiaca (Group 5, 2 isolates), one of each isolate as M. chalcea (Group 2) and M. maritima (Group 6). The isolate LK6-12 (Group 3) showed low similarity (99.16%) with the type strains of Micromonospora siamensis that will be the novel species of the genus Micromonospora. Meso-diaminopimelic acid (cell wall type II), xylose and arabinose (pattern D) were detected in their whole-cell hydrolysates. The major polar lipid was phosphatidylethanolamine (type II). The predominant cellular fatty acids were C<inf>17:0</inf>, C<inf>17:1</inf>ω8c, iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>15:0</inf>, and anteiso-C<inf>17:0</inf>. The predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>), or MK-10(H<inf>4</inf>). The DNA G+C contents of the isolates ranged from 71.6-72.6 mol%. On the primary screening, 2 isolates exhibited the antimicrobial activity against Bacillus subtilis ATCC 6633 and Kocuria rhizophila ATCC 9341.
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Rhizobium paknamense sp. nov., isolated from lesser duckweeds (Lemna aequinoctialis)
(2013-10-01)
Kittiwongwattana, Chokchai
;
Thawai, Chitti
A Gram-stain-negative, rod-shaped bacterium was isolated and designated strain L6-8T during a study of endophytic bacterial communities in lesser duckweed (Lemna aequinoctialis). Cells of strain L6-8T were motile with peritrichous flagella. The analysis of the nearly complete 16S rRNA gene sequence indicated that strain L6-8T was phylogenetically related to species of the genus Rhizobium. Its closest relatives were Rhizobium borbori DN316T (97.6 %), Rhizobium oryzae Alt 505T (97.3 %) and Rhizobium pseudoryzae J3-A127T (97.0 %). The sequence similarity analysis of housekeeping genes recA, glnII, atpD and gyrB showed low levels of sequence similarity (,91.5 %) between strain L6-8T and other species of the genus Rhizobium with validly published names. The pH range for growth was 4.0-9.0 (optimum 6.0-7.0), and the temperature range for growth was 20-45 °C (optimum 30 °C). Strain L6-8T tolerated NaCl up to 2% (w/v) (optimum 1% NaCl). The predominant components of cellular fatty acids were C<inf>19: 0</inf> cyclo ω8c (31.32 %), summed feature 8 (C<inf>18: 1</inf>ω7c and/or C<inf>18: 1</inf>ω6c; 25.39 %) and C<inf>16: 0</inf> (12.03 %). The DNA G+C content of strain L6-8T was 60.4 mol% (T<inf>m</inf>). nodC and nifH were not amplified in strain L6-8T. DNA-DNA relatedness between strain L6-8T and R. borbori DN316T, R. oryzae Alt505T and R. pseudoryzae J3-A127T was between 11.2 and 18.3 %. Based on the sequence similarity analyses, phenotypic, biochemical and physiological characteristics and DNA-DNA hybridization, strain L6-8T could be readily distinguished from its closest relatives and represents a novel species of the genus Rhizobium, for which the name Rhizobium paknamense sp. nov. is proposed. The type strain is L6-8T (=NBRC 109338T=BCC 55142T). © 2013 IUMS.
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Antimalarial and antitubercular C-glycosylated benz[α]anthraquinones from the marine-derived Streptomyces sp. BCC45596
(2012-09-01)
Supong, Khomsan
;
Thawai, Chitti
;
Suwanborirux, Khanit
;
Choowong, Wilunda
;
Supothina, Sumalee
Three naturally new C-glycosylated benz[α]anthraquinone derivatives, urdamycinone E (1), urdamycinone G (2), dehydroxyaquayamycin (3) have been isolated from the marine Streptomycetes sp. BCC45596. Urdamycin E (4), the possible biosynthetic precursor of 1-3, has also been identified after a re-cultivation of the strain. These compounds (1-4) exhibited potent anti-Plasmodium palcifarum K1 strain with IC <inf>50</inf> values in a range of 0.0534-2.93 μg/mL and anti-Mycobacterium tuberculosis with minimum inhibition concentrations (MICs) in a range of 3.13-12.50 μg/mL. Cytotoxicity against KB, MCF-7, NCI-H187, and Vero cells was also evaluated. © 2012 Phytochemical Society of Europe.
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Dactylosporangium tropicum sp. nov., isolated from soil
(2011-10-01)
Thawai, Chitti
;
Tanasupawat, Somboon
;
Kudo, Takuji
Two novel actinomycete strains, designated KB2-4 T and KB9-2, were isolated from soil samples. Both isolates formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid; the whole-cell sugars were galactose, glucose, mannose, rhamnose, ribose and xylose. The diagnostic phospholipid was phosphatidylethanolamine. The predominant menaquinones were MK-9(H8) and MK-9(H6). Mycolic acids were not detected. The predominant cellular fatty acids (>20 %) were iso-C <inf>16: 0</inf> and anteiso-C <inf>17: 0</inf>. For both strains, the G+C content of the genomic DNA was about 72 mol%. The morphological and chemotaxonomic characteristics of the two strains were typical of members of the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that these strains should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium salmoneum NRRL B-16294 T (99.1% 16S rRNA gene sequence similarity). DNA-DNA relatedness and some physiological and biochemical properties indicated that the isolates could be readily distinguished from their closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, the isolates represent a novel species, for which the name Dactylosporangium tropicum sp. nov. is proposed. The type strain is strain KB2-4T (=BCC 34760 T =JCM 15673 T). © 2011 IUMS.
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Micromonospora caldifontis sp. nov., isolated from hot spring soil
(2019-05-01)
Thawai, Chitti
;
Tanasupawat, Somboon
;
Kudo, Takuji
A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora. The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H<inf>6</inf>) and MK-10(H<inf>4</inf>). The major cellular fatty acids were iso-C<inf>16: 0</inf>, anteiso-C<inf>17: 0</inf>, C<inf>17: 0 </inf>and anteiso-C<inf>15: 0</inf>. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2%), Micromonospora eburnea DSM 44814T (98.2%) and Micromonospora spongicola S3-1T (98.1%). The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species. Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
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Agromyces tropicus sp. nov., isolated from soil
(2011-03-01)
Thawai, Chitti
;
Tanasupawat, Somboon
;
Suwanborirux, Khanit
;
Kudo, Takuji
Two actinomycete strains, CM9-9T and AK2-48, which produced straight rod-shaped, non-motile cells, were isolated from soils in Chiang Mai and Phuket provinces, respectively, Thailand. The morphological and chemotaxonomic characteristics of the isolates coincided with those of the genus Agromyces. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the isolates were clearly separated from their closest relative, Agromyces aurantiacus YIM 21741T, and should be classified in the genus Agromyces. Furthermore, a combination of DNA - DNA hybridization results and physiological and biochemical properties indicated that the isolates could be distinguished from all recognized members of the genus Agromyces. The isolates therefore represent a novel species, for which the name Agromyces tropicus sp. nov. is proposed. The type strain is CM9-9T (=JCM 15672T =BCC 34764T). © 2011 IUMS Printed in Great Britain.

Thawai, Chitti

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