Thawai, Chitti

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Thawai, Chitti
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chitti.th@kmitl.ac.th

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    Micromonospora solifontis sp. nov., an actinobacterium isolated from hot spring soil
    (2023-05-01)
    Duangupama, Thitikorn
    ;
    Pansomsuay, Rawirat
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain, PPF5-17<sup>T</sup>, was isolated from hot spring soil collected from Chiang Rai province, Thailand. The strain exhibited morphological and chemotaxonomic properties similar to those of members of the genus Micromonospora. Colonies of PPF5-17<sup>T</sup> were strong pinkish red and turned black after sporulation in ISP 2 agar medium. Cells formed single spores directly on the substrate mycelium. Growth was observed from 15 to 45 °C and at pH 5–8. Maximum NaCl concentration for growth was 3% (w/v). PPF5-17<sup>T</sup> was found to have meso-diaminopimelic acid, xylose, mannose and glucose in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannosides were observed as the membrane phospholipids. MK-10(H<inf>6</inf> ), MK-9(H<inf>6</inf> ), MK-10(H<inf>4</inf> ) and MK-9(H<inf>4</inf> ) were the major menaquinones. The predominant cellular fatty acids were iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>17:0</inf> and iso-C<inf>16:0</inf> . PPF5-17<sup>T</sup> shared the highest 16S rRNA gene sequence similarity with Micromonospora fluminis LMG 30467<sup>T</sup> (99.3%). A genome-based taxonomic study revealed that PPF5-17<sup>T</sup> was closely related to Micromonospora aurantinigra DSM 44815<sup>T</sup> in the phylogenomic tree with an average nucleotide identity by blast (ANIb) of 87.7% and a digital DNA–DNA hybridization (dDDH) value of, 36.1% which were below the threshold values for delineation of a novel species. Moreover, PPF5-17<sup>T</sup> could be distinguished from its closest neighbours, M. fluminis LMG 30467<sup>T</sup> and M. aurantinigra DSM 44815<sup>T</sup>, with respect to a broad range of phenotypic properties. Thus, PPF5-17<sup>T</sup> represents a novel species, for which the name Micromonospora solifontis sp. nov. is proposed. The type strain is PPF5-17<sup>T</sup> (= TBRC 8478<sup>T</sup> = NBRC 113441<sup>T</sup>).
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    Identification and antimicrobial activity of Micromonospora strains from Thai peat swamp forest soils
    (2018-04-01)
    Thawai, Chitti
    ;
    Kanchanasin, Pawina
    ;
    Ohkuma, Moriya
    ;
    Kudo, Takuji
    ;
    Tanasupawat, Somboon
    The identification and antimicrobial activity of thirteen actinomycete strains isolated from peat swamp forest soils collected from Narathiwat, Patthaloong and Yala provinces, the southern part of Thailand were carried out. Based on the phenotypic and chemotaxonomic characteristics, all isolates were belonged to genus Micromonospora. They were separated into six groups based on 16S ribosomal RNA gene sequence analysis and were identified as M. narathiwatensis (Group 1, 5 isolates), M. humi (Group 4, 3 isolates), M. aurantiaca (Group 5, 2 isolates), one of each isolate as M. chalcea (Group 2) and M. maritima (Group 6). The isolate LK6-12 (Group 3) showed low similarity (99.16%) with the type strains of Micromonospora siamensis that will be the novel species of the genus Micromonospora. Meso-diaminopimelic acid (cell wall type II), xylose and arabinose (pattern D) were detected in their whole-cell hydrolysates. The major polar lipid was phosphatidylethanolamine (type II). The predominant cellular fatty acids were C<inf>17:0</inf>, C<inf>17:1</inf>ω8c, iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>15:0</inf>, and anteiso-C<inf>17:0</inf>. The predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>), or MK-10(H<inf>4</inf>). The DNA G+C contents of the isolates ranged from 71.6-72.6 mol%. On the primary screening, 2 isolates exhibited the antimicrobial activity against Bacillus subtilis ATCC 6633 and Kocuria rhizophila ATCC 9341.
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    Micromonospora caldifontis sp. nov., isolated from hot spring soil
    (2019-05-01)
    Thawai, Chitti
    ;
    Tanasupawat, Somboon
    ;
    Kudo, Takuji
    A single spore forming actinomycete, designated strain HSS6-8<sup>T</sup>, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora. The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H<inf>6</inf>) and MK-10(H<inf>4</inf>). The major cellular fatty acids were iso-C<inf>16: 0</inf>, anteiso-C<inf>17: 0</inf>, C<inf>17: 0 </inf>and anteiso-C<inf>15: 0</inf>. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8<sup>T</sup> was closely related to Micromonospora nigra DSM 43818<sup>T</sup> (98.2%), Micromonospora eburnea DSM 44814<sup>T</sup> (98.2%) and Micromonospora spongicola S3-1<sup>T</sup> (98.1%). The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8<sup>T</sup> from its related species. Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8<sup>T</sup> (=TBRC 8927<sup>T</sup>=JCM 17126<sup>T</sup>).
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    Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the Gulf of Thailand
    (2013-09-01)
    Supong, Khomsan
    ;
    Suriyachadkun, Chanwit
    ;
    Pittayakhajonwut, Pattama
    ;
    Suwanborirux, Khanit
    ;
    Thawai, Chitti
    An actinomycete strain, S3-1 T, was isolated from marine sponge sample collected from the Gulf of Thailand. The strain is aerobic, Gram-positive and produced single spores at the tip of the substrate mycelium. Strain S3-1 T contained meso-diaminopimelic acid in the peptidoglycan, whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain S3-1 T consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipid and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain were identified as a member of the genus Micromonospora. Phylogenetic analysis based on 16S rRNA gene sequence analysis of the strain showed similarity to Micromonospora nigra DSM 43818 T (98.8%), Micromonospora yangpuensis FXJ6.011 T (98.7%) and Micromonospora narathiwatensis BTG4-1 T (98.6%). The DNA G+C content was 72.7 mol%. The phenotypic characteristics and DNA-DNA relatedness values supported that the classification of this strain as a novel species in the genus Micromonospora, for which the name Micromonospora spongicola sp. nov. (type strain S3-1 T =BCC 45595 T =NBRC 108779 T) is proposed. © 2013 Japan Antibiotics Research Association All rights reserved.
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    Micromonospora soli sp. nov., isolated from rice rhizosphere soil
    (2016-03-01)
    Thawai, Chitti
    ;
    Kittiwongwattana, Chokchai
    ;
    Thanaboripat, Dusanee
    ;
    Laosinwattana, Chamroon
    ;
    Koohakan, Prommart
    An actinomycete strain SL3-70<sup>T</sup> was isolated from a rice field and characterised using a polyphasic approach. The morphological and chemotaxonomical characteristics of strain SL3-70<sup>T</sup> indicate that it belongs to the genus Micromonospora. The phylogenetic analysis of the nearly complete 16S rRNA gene sequence revealed that strain SL3-70<sup>T</sup> is a member of the genus Micromonospora, and is closely related to Micromonospora echinaurantica DSM 43904<sup>T</sup> (99.1 % 16S rRNA gene sequence similarity) and Micromonospora kangleipakensis MBRL 34<sup>T</sup> (98.8 %). DNA–DNA relatedness between strain SL3-70<sup>T</sup> and its relatives ranged from 21.2 % ± 0.6 to 38.7 % ± 0.4. The results obtained from our study indicate that strain SL3-70<sup>T</sup> represents a novel species of the genus Micromonospora, for which the name Micromonospora soli sp. nov. is proposed. The type strain is SL3-70<sup>T</sup> (=BCC 67268<sup>T</sup>; =NBRC 110009<sup>T</sup>).
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    Micromonospora thermarum sp. nov., an actinobacterium isolated from hot spring soil
    (2023-04-01)
    Pansomsuay, Rawirat
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinomycete, designated strain HSS6-12<sup>T</sup>, was isolated from hot spring sediment collected from Ranong province, Thailand. The strain showed taxonomic characteristics consistent with those of members of the genus Micromonospora. HSS6-12<sup>T</sup> produced a single spore directly on the substrate mycelium, and no aerial mycelium was detected. The isomer of diamino acid presented in cell wall peptidoglycan was meso-diaminopimelic acid. Arabinose, xylose, glucose, and ribose were detected in whole-cell hydrolysates. MK-10(H<inf>4</inf>), MK-9(H<inf>4</inf>), and MK-10(H<inf>6</inf>) were major menaquinones. Major cellular fatty acids were iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf>, and iso-C<inf>17:0</inf>. Phospholipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylinositolmannosides. 16S rRNA gene analysis revealed that HSS6-12<sup>T</sup> shared the highest 16S rRNA gene sequence similarity with Micromonospora inositola DSM 43819<sup>T</sup> (99.3%). In contrast, the genome analysis showed that HSS6-12<sup>T</sup> formed a tight taxonomic position in a phylogenomic tree with Micromonospora endolithica DSM 44398<sup>T</sup>. Moreover, the average nucleotide identity-blast, the digital DNA-DNA hybridization, and the average amino acid identity values between HSS6-12<sup>T</sup> and M. inositola DSM 43819<sup>T</sup> and M. endolithica DSM 44398<sup>T</sup> were 83.1–84.0%, 27.5–28.7%, and 80.4–82.2%, respectively, indicating that HSS6-12<sup>T</sup> was different species with both closely related Micromonospora-type strains. In addition, HSS6-12<sup>T</sup> could be discriminated from its closely related type strains by many physiological and biochemical characteristics. Thus, HSS6-12<sup>T</sup> could be considered a novel species of the genus Micromonospora, and the name Micromonospora thermarum is proposed for the strain. The type strain is HSS6-12<sup>T</sup> (= BCC 41915<sup>T</sup> = JCM 17127<sup>T</sup>).