Thawai, Chitti

Preferred name

Thawai, Chitti

Main Affiliation

School of Science

chitti.th@kmitl.ac.th

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A novel diterpene agent isolated from Microbispora hainanensis strain CSR-4 and its in vitro and in silico inhibition effects on acetylcholine esterase enzyme
(2020-12-01)
Thawai, Chitti
;
Bunbamrung, Nantiya
;
Pittayakhajonwut, Pattama
;
Chongruchiroj, Sumet
;
Pratuangdejkul, Jaturong
An actinomycete strain CSR-4 was isolated from the rhizosphere soil of Zingiber montanum. Taxonomic characterization revealed strain CSR-4 was a member of the genus Microbispora. Whole-genome sequence analysis exhibited the highest average nucleotide identity (ANI) value (95.34%) and digital DNA–DNA hybridization (DDH) value (74.7%) between strain CSR-4 and the closest relative M. hainanensis DSM 45428T, which was in line with the assignment to same species. In addition, a new diterpene compound, 2α-hydroxy-8(14), 15-pimaradien-17, 18-dioic acid, and nine known compounds were isolated from the ethyl acetate crude extract of fermentation broth. Interestingly, a new diterpene displayed the suppressive effect on the recombinant human acetylcholinesterase (rhAChE) enzymes (IC<inf>50</inf> 96.87 ± 2.31 μg/ml). In silico studies based on molecular docking and molecular dynamics (MD) simulations were performed to predict a binding mode of the new compound into the binding pocket of the rhAChE enzyme and revealed that some amino acids in the peripheral anions site (PAS), anionic subsite, oxyanion site and catalytic active site (CAS) of the rhAChE have interacted with the compound. Therefore, our new compound could be proposed as a potential active human AChE inhibitor. Moreover, the new compound can protect significantly the neuron cells (% neuron viability = 88.56 ± 5.19%) from oxidative stress induced by serum deprivation method at 1 ng/ml without both neurotoxicities on murine P19-derived neuron cells and cytotoxicity against Vero cells.
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Micromonospora solifontis sp. nov., an actinobacterium isolated from hot spring soil
(2023-05-01)
Duangupama, Thitikorn
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Pansomsuay, Rawirat
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Pittayakhajonwut, Pattama
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Intaraudom, Chakapong
;
Suriyachadkun, Chanwit
An actinobacterium strain, PPF5-17T, was isolated from hot spring soil collected from Chiang Rai province, Thailand. The strain exhibited morphological and chemotaxonomic properties similar to those of members of the genus Micromonospora. Colonies of PPF5-17T were strong pinkish red and turned black after sporulation in ISP 2 agar medium. Cells formed single spores directly on the substrate mycelium. Growth was observed from 15 to 45 °C and at pH 5–8. Maximum NaCl concentration for growth was 3% (w/v). PPF5-17T was found to have meso-diaminopimelic acid, xylose, mannose and glucose in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannosides were observed as the membrane phospholipids. MK-10(H<inf>6</inf> ), MK-9(H<inf>6</inf> ), MK-10(H<inf>4</inf> ) and MK-9(H<inf>4</inf> ) were the major menaquinones. The predominant cellular fatty acids were iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>17:0</inf> and iso-C<inf>16:0</inf> . PPF5-17T shared the highest 16S rRNA gene sequence similarity with Micromonospora fluminis LMG 30467T (99.3%). A genome-based taxonomic study revealed that PPF5-17T was closely related to Micromonospora aurantinigra DSM 44815T in the phylogenomic tree with an average nucleotide identity by blast (ANIb) of 87.7% and a digital DNA–DNA hybridization (dDDH) value of, 36.1% which were below the threshold values for delineation of a novel species. Moreover, PPF5-17T could be distinguished from its closest neighbours, M. fluminis LMG 30467T and M. aurantinigra DSM 44815T, with respect to a broad range of phenotypic properties. Thus, PPF5-17T represents a novel species, for which the name Micromonospora solifontis sp. nov. is proposed. The type strain is PPF5-17T (= TBRC 8478T = NBRC 113441T).
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Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)
(2015-11-01)
Thanaboripat, Dusanee
;
Thawai, Chitti
;
Kittiwongwattana, Chokchai
;
Laosinwattana, Chamroon
;
Koohakan, Prommart
An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T).
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Asanoa endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Boesenbergia rotunda
(2016-03-01)
Niemhom, Nantawan
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Chutrakul, Chanikul
;
Suriyachadkun, Chanwit
;
Thawai, Chitti
A novel Gram-stain-positive, non-motile, endophytic actinomycete, designated strain BR3-1T, which produced spore chains borne on the tips of short sporophores, was isolated from the rhizome of Boesenbergia rotunda collected from Udon Thani province, Thailand. This strain was investigated for its taxonomic position using a polyphasic approach. The strain contained 3-hydroxydiaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars comprised glucose, mannose, rhamnose, ribose and xylose. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides were found as the characteristic phospholipids. The predominant menaquinones were MK-10(H<inf>8</inf>) and MK-10(H<inf>6</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf> and anteiso-C<inf>15:0</inf>. The G+C content of the genomic DNA was 71.4 mol%. 16S rRNA gene sequence analysis revealed that strain BR3-1T belonged to the genus Asanoa and was most closely related to Asanoa ishikariensis (99.39%), Asanoa iriomotensis (99.31%), Asanoa siamensis (99.17%), Asanoa ferruginea (98.84%) and Asanoa hainanensis (98.71%). The DNA–DNA relatedness value between strain BR3-1Tand its phylogenetically closest relatives was in the range of 15.4%±1.2 to 45.8%±2.6. In addition, some physiological and biochemical properties indicated that strain BR3-1T could be readily distinguished from all type strains in the genus Asanoa. Thus, strain BR3-1T should be classified as a representative of a novel species, for which the name Asanoa endophytica sp. nov. is proposed. The type strain is BR3-1T(5BCC 66355T=NBRC 110002T).
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Description of Streptomyces siderophoricus sp. nov., a promising nocardamine-producing species isolated from the rhizosphere soil of Mangifera indica
(2024-11-01)
Duangupama, Thitikorn
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Pittayakhajonwut, Pattama
;
Intaraudom, Chakapong
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Suriyachadkun, Chanwit
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Tadtong, Sarin
An actinomycete, designated strain CH9-7T, was isolated from the rhizosphere soil of Mangifera indica. The morphological and chemotaxonomic properties, such as the production of spiral spore chains and the presence of LL-diaminopimelic acid in the peptidoglycan, showed that it belongs to the genus Streptomyces. Based on the 16S rRNA gene analysis, it was confirmed that strain CH9-7T was a member of the genus Streptomyces and revealed 99.9% 16S rRNA gene sequence similarity to its closest relative strains, Streptomyces lydicus NBRC 13058 T and Streptomyces chattanoogensis NBRC 12754 T. Although the strain showed high 16S rRNA gene sequence similarity values, however, genome relatedness indexes exhibited that the average nucleotide identity based on the MUMmer (ANIm) algorithm, the average amino acid identity (AAI), and the digital DNA–DNA hybridization values between strain CH9-7T and its closest phylogenomic relatives were below the threshold values for delineation of a novel species, (ANIm ranging from 87.5 to 88.6, AAI ranging from 80.6 to 84.6, and dDDH ranging from 28.4 to 31.7), respectively. A taxonomic position of strain CH9-7T in the phylogenomic tree showed that the closest relative strain was S. lydicus NBRC 13058 T. The comparative phenotypic studies between strain CH9-7T and its closest relatives revealed that strain CH9-7T could be classified as a novel species of the genus Streptomyces. Thus, the name Streptomyces siderophoricus sp. nov. is proposed for the strain. The type strain is CH9-7T (= TBRC 17833 T = NBRC 116426 T). The chemical investigation led to the isolation of four known compounds (compounds 1-4). Among these compounds, compound 1 was identified to be nocardamine, a promising bioactive substance.
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Jishengella zingiberis sp. Nov., isolated from root tissue of zingiber montanum
(2018-10-01)
Thawai, Chitti
;
He, Ya Wen
;
Tadtong, Sarin
A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose. The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol. The major menaquinone was MK-9 (H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>15: 0</inf> and C<inf>17: 1</inf> w8c. The G+C content of the genomic DNA was 70.6 mol%. 16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %). Based on the data of physiological and biochemical tests, including the result of DNA–DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengella zingiberis sp. nov. is proposed. The type strain is PLAI 1-1T (=TBRC 7644T =NBRC 113144T ).
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Dactylosporangium maewongense sp. nov., isolated from soil
(2010-05-01)
Chiaraphongphon, Suthamat
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Suriyachadkun, Chanwit
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Tamura, Tomohiko
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Thawai, Chitti
Morphological and chemotaxonomic characterization of actinomycete strain MW2-25T, isolated from tropical forest soil in Nakhon Sawan Province, Thailand, clearly demonstrated that this strain belongs to the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that this strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium aurantiacum IFO 12592T (99.3% sequence similarity). DNA-DNA hybridization values and some physiological and biochemical properties indicated that this strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Dactylosporangium maewongense sp. nov. is proposed. The type strain is MW2-25T (=BCC 34832T=JCM 15933T). © 2010 IUMS.
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Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.)
(2022-01-01)
Duangupama, Thitikorn
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Pittayakhajonwut, Pattama
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Intaraudom, Chakapong
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Suriyachadkun, Chanwit
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Sirirote, Pramote
A member of the genus Streptomyces, designated RCPT1-4T, was isolated from compost of Senna siamea (Lam.), collected from an agricultural area in Rayong province, Thailand. The spore morphology and the presence of ll-diaminopimelic acid in the peptidoglycan indicate that RCPT1-4T shows the typical properties of members of the genus Streptomyces. On the basis of the results of 16S rRNA gene sequence analysis, the strain should be classified as representing a member of the genus Streptomy-ces and was most closely related to Streptomyces fumigatiscleroticus NBRC 12999T with the highest 16S rRNA gene sequence similarity of 99.2%, followed by Streptomyces spiralis NBRC 14215T (99.0%). In addition, RCPT1-4T shared the highest average nucleotide identity by blast (ANIb) (86.0%), and digital DNA–DNA hybridization (dDDH) (32.1%) values with S. spiralis NBRC 14215T. Furthermore, several physiological and biochemical differences were observed between RCPT1-4T and the closely related type strains of species with validly published names. These taxonomic data indicated that RCPT1-4T could be considered to represent a novel species of the genus Streptomyces and the name Streptomyces sennicomposti sp. nov. is proposed for this strain. The type strain is RCPT1-4T (=TBRC 11260T=NBRC 114303T).
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Nocardia coffeae sp. nov., an endophytic actinobacterium isolatefrom the root of Coffea arabica (L.)
(2022-01-01)
Nammali, Achararak
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Duangupama, Thitikorn
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Suriyachadkun, Chanwit
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Tanasupawat, Somboon
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Thawai, Chitti
The polyphasic taxonomic study of a novel endophytic actinobacterium strain (CA2R105T) was carried out. The strain formed fragmented substrate mycelium and showed chemotaxonomic properties typical of members of the genus Nocardia, i.e. the presence of mycolic acid and MK-8 (H<inf>4ω-cycl</inf> ) in its cells. Strain CA2R105T exhibited the highest 16S rRNA gene sequence similarity to Nocardia jiangxiensis NBRC 101359T (99.2%). The genome-based taxonomic analysis revealed low average nucleotide identity-blast and digital DNA–DNA hybridization values (<93.7, and <65.2%, respectively) to its closest relative. Moreover, many different phenotypic characteristics were observed between strain CA2R105T and all related Nocardia-type strains. This taxonomic evidence suggested that strain CA2R105T should be judged as representing a novel species of the genus Nocardia and the name, Nocardia coffeae sp. nov. is proposed. The type strain is CA2R105T (=TBRC 11247T=NBRC 114292T).
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Identification and antimicrobial activity of Micromonospora strains from Thai peat swamp forest soils
(2018-04-01)
Thawai, Chitti
;
Kanchanasin, Pawina
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Ohkuma, Moriya
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Kudo, Takuji
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Tanasupawat, Somboon
The identification and antimicrobial activity of thirteen actinomycete strains isolated from peat swamp forest soils collected from Narathiwat, Patthaloong and Yala provinces, the southern part of Thailand were carried out. Based on the phenotypic and chemotaxonomic characteristics, all isolates were belonged to genus Micromonospora. They were separated into six groups based on 16S ribosomal RNA gene sequence analysis and were identified as M. narathiwatensis (Group 1, 5 isolates), M. humi (Group 4, 3 isolates), M. aurantiaca (Group 5, 2 isolates), one of each isolate as M. chalcea (Group 2) and M. maritima (Group 6). The isolate LK6-12 (Group 3) showed low similarity (99.16%) with the type strains of Micromonospora siamensis that will be the novel species of the genus Micromonospora. Meso-diaminopimelic acid (cell wall type II), xylose and arabinose (pattern D) were detected in their whole-cell hydrolysates. The major polar lipid was phosphatidylethanolamine (type II). The predominant cellular fatty acids were C<inf>17:0</inf>, C<inf>17:1</inf>ω8c, iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>15:0</inf>, and anteiso-C<inf>17:0</inf>. The predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>), or MK-10(H<inf>4</inf>). The DNA G+C contents of the isolates ranged from 71.6-72.6 mol%. On the primary screening, 2 isolates exhibited the antimicrobial activity against Bacillus subtilis ATCC 6633 and Kocuria rhizophila ATCC 9341.

Thawai, Chitti

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