Srikijkasemwat, Kanokrat

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Srikijkasemwat, Kanokrat
Alternative Name
Srikijkasemwat, K.
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Email
kanokrat.sr@kmitl.ac.th

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    Efficacy of two primer sets used in the sex identification of rufous-winged buzzard (Butastur liventer)
    (2021-01-01)
    Kaewhom, Paitoon
    ;
    Srikijkasemwat, Kanokrat
    Background: The rufous-winged buzzard (Butastur liventer) belongs to the order Accipitriformes, which is monomorphic, resulting in the difficulty to identify the gender. However, sex determination is important for predicting the sex ratio of this buzzard in nature in order to avoid its extinction. Aim: We aimed to develop a primer set that is able to sex the rufous-winged buzzard through polymerase chain reaction (PCR) amplification and compare the efficacy of the two sets of primers by using PCR technique. Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on the visual appearance of the bands. Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67, and 1 ng/µl. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the chromo-helicase DNA binding (CHD) gene of known gender buzzards using the PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds. Results: The lowest concentration of the DNA template where P2/NP/MP could amplify DNA fragments was 1 ng/µl, and this set of primers could identify the gender of all birds correctly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µl, and it had only 78% specificity. Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.
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    A molecular survey of Theileria spp. in Ruminants in the Thailand-Cambodia border region
    (2022-01-01)
    Kaewhom, P.
    ;
    Srikijkasemwat, K.
    Theileriosis is caused by Theileria spp. Parasites which transmitted by various species of ticks including Amblyomma, Haemaphysalis, Hyalomma and Rhipicephalus. This pathogen can be found in red blood cells and white blood cells of farmed animals, including cattle, buffaloes, goats and sheep. Theileria spp. leads to a high morbidity in small ruminants, economic loss in agricultural production and time spent on infection prevention. It is necessary to detect the infection in a farm, as there are generally no clearly recognisable clinical signs. Our results revealed that infected animals displayed 230 bp DNA fragments, which is the length of the V4 region of the 18S rRNA gene of Theileria spp. The overall prevalence of Theileria spp. in ruminants farmed in Sa Kaeo Province, the border between Thailand and Cambodia, was 9% (29/314). The prevalence of Theileria spp. in meat cattle (6%), buffaloes(6%), meat goats (6%) and crossbred meat sheep (6%). It was also found that the prevalence of Theileria spp. was linked to neither species nor gender (p>0.05). Polymerase Chain Reaction was used to analyse Theileria spp. since it is very sensitive, specific and fast. Our analysis can be used to prevent and control the spread of Theileria infection in ruminants. Furthermore, this information may guide implemention of a policy on animal movement within the border regions.