Srikijkasemwat, Kanokrat

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Preferred name
Srikijkasemwat, Kanokrat
Alternative Name
Srikijkasemwat, K.
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Email
kanokrat.sr@kmitl.ac.th

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    Coexistent infection event of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and Pasteurella multocida in swine
    (2024-07-01)
    Thammakarn, C.
    ;
    Pham, H. S.H.
    ;
    Kaewhom, P.
    ;
    Klompanya, A.
    ;
    Srikijkasemwat, K.
    Result elucidated the instances of concurrent infections by crucial pathogens in pigs, which is adversely impacted the herd's health status on a farm located in the Rachaburi province of Thailand. The farm which accommodated a population of 700 sows had found its fattening pig units exhibited unsatisfactory growth performance and an inconsistent size appearance. The serum samples underwent the testing via enzyme linked immunosorbent assay, unveiled a substantial antibody response to the porcine reproductive and respiratory syndrome virus (PRRSV). The finding strongly indicated the prevalence of PRRSV infection within the farm. Furthermore, analysis of lymph node samples confirmed the presence of porcine circovirus type 2 (PCV2) through conventional polymerase chain reaction. The bacterial culture carried out on lung samples which detected an infection caused by Pasteurella multocida. In summary, the investigation is conclusively established the occurrence of a mixed infection involving both viruses and bacteria on this farm, specifically PRRSV, PCV2, and P. multocida. The report is illustrated the possibility of multiple infections by viruses and bacteria, which can potentially undermine the efficacy of vaccinations and cause severe clinical signs on the farm.
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    Antimicrobial resistance in fecal Escherichia coli from different pig production systems
    (2022-01-01)
    Mitchaothai, Jamlong
    ;
    Srikijkasemwat, Kanokrat
    Objective: The objective of the current study was to investigate the influences of conventional (CO) and deep litter (DE) systems on antimicrobial resistance in fecal Escherichia coli (E. coli). Methods: A crosssectional study was carried out to detect antimicrobial resistance to E. coli in swine fecal samples in CO and DE systems located in western and northeastern Thailand. Individual rectal swab samples were taken only from healthy pigs. A total of 215 individual and healthy pigs were randomly selected for isolation and antimicrobial susceptibility test of E. coli by the disc diffusion method. The test panel included amoxicillin (AMX), colistin, doxycycline (DOX), enrofloxacin, gentamicin (GEN), kanamycin, neomycin (NEO), and trimethoprimsulfamethoxazole (SXT). Results: There were significant (p<0.05) lower resistance levels for GEN, NEO, and SXT in the DE farms compared to those in the CO farms. There was a lower number of antimicrobial resistance agents (p<0.001) in the DE farms compared to those in the CO farms. This result was consistent with those in western (p<0.01) and northeastern (p<0.01) Thailand. Overall, antibiograms of AMXSXT and AMXDOXSXT were found in the CO (19.09% and 20.91%, respectively) and the DE (16.19% and 24.76%, respectively) farms. No antimicrobial resistance (5.71%) was found and AMX (13.33%) resistant pigs in the DE farms, whereas the pattern of AMXGENSXT (6.36%) and AMXDOXGENSXT (11.82%) resistant pigs was found in the CO farms. Conclusion: The DE system for pig farming was superior to conventional pig farming by lowering the resistance level of fecal E. coli to GEN, NEO, and SXT, with decreasing the number of antimicrobial resistance agents and inducing a small proportion of pigs to be free from antimicrobial resistance.