Polyorach, Sineenart

Preferred name

Alternative Name

Polyorach, S.

Main Affiliation

School of Agricultural Technology

sineenart.po@kmitl.ac.th

Now showing 1 - 5 of 5

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Improving the quality of sugarcane bagasse by urea and calcium hydroxide on gas production, degradability and rumen fermentation characteristics
(2017-12-01)
Gunun, N.
;
Gunun, P.
;
Wanapat, M.
;
Cherdthong, A.
;
Kang, S.
The objective of this study was to determine the influence of urea and whole soybean meal or calcium hydroxide [Ca(OH)<inf>2</inf>] treatment on sugarcane bagasse nutritive values and in vitro fermentation. The experimental design was a completely randomized design (CRD) and the dietary treatments were sugarcane bagasse treated with urea (0, 20, 40g/kg) and whole soybean meal or Ca(OH)<inf>2</inf> (0, 25, 50g/kg). It was found that the protein content of sugarcane bagasse was significantly increased by whole soybean meal and urea treatment, and the values ranged from 65-71 g/kg DM. On the other hand, treatment of Ca(OH)<inf>2</inf> 50 g/kg with urea 40 g/kg could reduce (p<0.05) fiber content of sugarcane bagasse and the values were 533, 514 and 104 g/kg DM for NDF, ADF and ADL, respectively. Gas production from the insoluble fraction (b), potential extent of gas production (a+b) and cumulative gas production were significantly increased (p<0.05) in sugarcane bagasse treated with urea 40 g/kg and urea 40 g/kg + whole soybean meal 25 g/kg (55.3, 53.5 and 50.1 ml), respectively. Urea and Ca(OH)<inf>2</inf> treatment could increase DM and OM degradability, true digestibility and microbial mass of sugarcane bagasse(p<0.05). Furthermore, the concentration of C3, C2 and C2:C3 were improved by urea and whole soybean meal or Ca(OH)<inf>2</inf> treatment. In addition, methane production was decreased in sugarcane bagasse treated with urea 40 g/kg in the present study. Based on this experiment, it could be concluded that treatment of urea and Ca(OH)<inf>2</inf> was an alternative method to improve the nutritive value of sugarcane bagasse, gas production, digestibility and ruminal fermentation characteristics.
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Optimal cultivation time for yeast and lactic acid bacteria in fermented milk and effects of fermented soybean meal on rumen degradability using nylon bag technique
(2016-09-01)
Polyorach, S.
;
Poungchompu, O.
;
Wanapat, M.
;
Kang, S.
;
Cherdthong, A.
The objectives of this study were to determine an optimal cultivation time for populations of yeast and lactic acid bacteria (LAB) co-cultured in fermented milk and effects of soybean meal fermented milk (SBMFM) supplementation on rumen degradability in beef cattle using nylon bag technique. The study on an optimal cultivation time for yeast and LAB growth in fermented milk was determined at 0, 4, 8, 24, 48, 72, and 96 h post-cultivation. After fermenting for 4 days, an optimal cultivation time of yeast and LAB in fermented milk was selected and used for making the SBMFM product to study nylon bag technique. Two ruminal fistulated beef cattle (410±10 kg) were used to study on the effect of SBMFM supplementation (0%, 3%, and 5% of total concentrate substrate) on rumen degradability using in situ method at incubation times of 0, 2, 4, 6, 12, 24, 48, and 72 h according to a Completely randomized design. The results revealed that the highest yeast and LAB population culture in fermented milk was found at 72 h-post cultivation. From in situ study, the soluble fractions at time zero (a), potential degradability (a+b) and effective degradability of dry matter (EDDM) linearly (p<0.01) increased with the increasing supplemental levels and the highest was in the 5% SBMFM supplemented group. However, there was no effect of SBMFM supplement on insoluble degradability fractions (b) and rate of degradation (c). In conclusion, the optimal fermented time for fermented milk with yeast and LAB was at 72 h-post cultivation and supplementation of SBMFM at 5% of total concentrate substrate could improve rumen degradability of beef cattle. However, further research on effect of SBMFM on rumen ecology and production performance in meat and milk should be conducted using in vivo both digestion and feeding trials.
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In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins
(2017-01-01)
Supapong, C.
;
Cherdthong, A.
;
Seankamsorn, A.
;
Khonkhaeng, B.
;
Wanapat, M.
The aim of the study was to evaluate the effect of supplementation of Delonix regia (DR) seed meal containing tannins and saponins on gas kinetics, ammonia-nitrogen (NH3-N) content, pH, methane (CH4) production and dry matter (DM) digestibility using an in vitro gas production technique. The experimental design was completely randomized, and the dietary treatments included DR seed meal supplementation at levels of 0, 3.3, 5.0, 6.7, 8.3, 10, 11.7, 13.3, 15.0 and 16.7 mg DM added to 0.5 g of roughage and concentrate (70:30) mixture. The gas production was measured at several time points: 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h by a pressure transducer. The parameters of gas kinetics and cumulative gas production were not altered (P > 0.05), except for gas production rate constant for the insoluble fraction which was the highest at 11.7 mg DR seed meal inclusion. CH4 production and total protozoa counts linearly decreased with increasing DR seed meal levels (P < 0.05). DR seed meal addition caused quadratic increase of in vitro DM digestibility with the highest value at 11.7 mg DR seed meal inclusion. No significant difference in volatile fatty acid profile (P > 0.05) was stated between treatments except for propionic acid. In conclusion, supplementation of DR seed meal resulted in improved in vitro gas kinetics and DM digestibility up to 11.7 mg level, while CH4 production was reduced linearly. The further in vivo studies are necessary to examine practical of DR seed meal usage in animal production.
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Effects of microbial fermented liquid (MFL) supplementation on gas production kinetics and digestibility using in vitro gas production technique
(2018-12-01)
Nampukdee, R.
;
Polyorach, S.
;
Wanapat, M.
;
Kang, S.
;
Cherdthong, A.
The effects of microbial fermented liquid (MFL) supplementation on rumen fermentation and digestibility of dairy steer using in vitro gas production technique was studied. The two rumen fistulated dairy steers as a rumen fluid donor was used. The levels of source of microbes (yeast (Y) and microbial fermented liquid (MFL) were incorporated with the levels of supplementation (0, 10, 20 and 30 % of concentrate) was recorded. It was found that the intercept value (a) and IVDMD and IVOMD were interacted (p<0.01) between microbial source and supplement levels, while, supplement levels affected on a and the insoluble fraction (b), microbial source affected on b, potential extent of gas production (a+b) and cumulative gas production at 72 h and IVOMD. Moreover, supplementation of MFL with 20 % of concentrate were the highest (p<0.05) of b, c, a+b, cumulative gas production at 72 h, IVDMD and IVOMD. In conclusion, supplementation of MFL could improve nutritional digestibility and a possible productivity in ruminants.
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Effect of Delonix regia seed meal supplementation in Thai native beef cattle on feed intake, rumen fermentation characteristics and methane production
(2017-10-01)
Supapong, C.
;
Cherdthong, A.
;
Seankamsorn, A.
;
Khonkhaeng, B.
;
Wanapat, M.
The aim of this research was to investigate the effect of supplementing Delonix regia (DR) seed meal on feed intake, digestibility, rumen fermentation, nitrogen balance and CH<inf>4</inf> production in Thai native beef cattle fed on rice straw. Four Thai native beef cattle with the initial body weight (BW) of 100 ± 5.0 kg were randomly assigned according to a 4 × 4 Latin square design to receive DR seed meal supplementation at 0, 90, 180 and 270 g/d. The present results revealed that the total intake (g/kg BW<sup>0.75</sup>) was significantly increased with the inclusion of dry matter (DM) seed meal at 270 g (P < 0.05). DM and OM digestibility were decreased when increasing DR seed meal levels (P < 0.05). Ruminal NH<inf>3</inf>-N concentration increased in beef cattle receiving DR seed meal. Supplementation of DR seed meal did not alter fungal zoospores’ concentration (P > 0.05), whereas the protozoal population was at 0, 4 h post feeding, and the mean values reduced when increasing the levels of DR seed meal supplemented (P < 0.05). The concentration of propionic acid at 4 h post feeding and its average concentration were significantly highest when 270 g DR seed meal was supplemented (P < 0.05). Estimation of CH<inf>4</inf> concentrations and CH<inf>4</inf> per dry matter intake were found reduced when increasing its DR seed meal concentration. In addition, N absorption, N retention and proportion of N retention to N intake were enhanced when 270 g DR seed meal was supplemented (P < 0.05). Thus, the inclusion of DR seed meal at 270 g/d resulted in improving total feed intake, rumen fermentation and N balance whereas there was reduced DM digestibility, protozoal population and CH<inf>4</inf> production in beef cattle fed rice straw base.

Polyorach, Sineenart

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