Polyorach, Sineenart

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Polyorach, Sineenart
Alternative Name
Polyorach, S.
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Email
sineenart.po@kmitl.ac.th

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    Improving the quality of sugarcane bagasse by urea and calcium hydroxide on gas production, degradability and rumen fermentation characteristics
    (2017-12-01)
    Gunun, N.
    ;
    Gunun, P.
    ;
    Wanapat, M.
    ;
    Cherdthong, A.
    ;
    Kang, S.
    The objective of this study was to determine the influence of urea and whole soybean meal or calcium hydroxide [Ca(OH)<inf>2</inf>] treatment on sugarcane bagasse nutritive values and in vitro fermentation. The experimental design was a completely randomized design (CRD) and the dietary treatments were sugarcane bagasse treated with urea (0, 20, 40g/kg) and whole soybean meal or Ca(OH)<inf>2</inf> (0, 25, 50g/kg). It was found that the protein content of sugarcane bagasse was significantly increased by whole soybean meal and urea treatment, and the values ranged from 65-71 g/kg DM. On the other hand, treatment of Ca(OH)<inf>2</inf> 50 g/kg with urea 40 g/kg could reduce (p<0.05) fiber content of sugarcane bagasse and the values were 533, 514 and 104 g/kg DM for NDF, ADF and ADL, respectively. Gas production from the insoluble fraction (b), potential extent of gas production (a+b) and cumulative gas production were significantly increased (p<0.05) in sugarcane bagasse treated with urea 40 g/kg and urea 40 g/kg + whole soybean meal 25 g/kg (55.3, 53.5 and 50.1 ml), respectively. Urea and Ca(OH)<inf>2</inf> treatment could increase DM and OM degradability, true digestibility and microbial mass of sugarcane bagasse(p<0.05). Furthermore, the concentration of C3, C2 and C2:C3 were improved by urea and whole soybean meal or Ca(OH)<inf>2</inf> treatment. In addition, methane production was decreased in sugarcane bagasse treated with urea 40 g/kg in the present study. Based on this experiment, it could be concluded that treatment of urea and Ca(OH)<inf>2</inf> was an alternative method to improve the nutritive value of sugarcane bagasse, gas production, digestibility and ruminal fermentation characteristics.
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    Optimal cultivation time for yeast and lactic acid bacteria in fermented milk and effects of fermented soybean meal on rumen degradability using nylon bag technique
    (2016-09-01)
    Polyorach, S.
    ;
    Poungchompu, O.
    ;
    Wanapat, M.
    ;
    Kang, S.
    ;
    Cherdthong, A.
    The objectives of this study were to determine an optimal cultivation time for populations of yeast and lactic acid bacteria (LAB) co-cultured in fermented milk and effects of soybean meal fermented milk (SBMFM) supplementation on rumen degradability in beef cattle using nylon bag technique. The study on an optimal cultivation time for yeast and LAB growth in fermented milk was determined at 0, 4, 8, 24, 48, 72, and 96 h post-cultivation. After fermenting for 4 days, an optimal cultivation time of yeast and LAB in fermented milk was selected and used for making the SBMFM product to study nylon bag technique. Two ruminal fistulated beef cattle (410±10 kg) were used to study on the effect of SBMFM supplementation (0%, 3%, and 5% of total concentrate substrate) on rumen degradability using in situ method at incubation times of 0, 2, 4, 6, 12, 24, 48, and 72 h according to a Completely randomized design. The results revealed that the highest yeast and LAB population culture in fermented milk was found at 72 h-post cultivation. From in situ study, the soluble fractions at time zero (a), potential degradability (a+b) and effective degradability of dry matter (EDDM) linearly (p<0.01) increased with the increasing supplemental levels and the highest was in the 5% SBMFM supplemented group. However, there was no effect of SBMFM supplement on insoluble degradability fractions (b) and rate of degradation (c). In conclusion, the optimal fermented time for fermented milk with yeast and LAB was at 72 h-post cultivation and supplementation of SBMFM at 5% of total concentrate substrate could improve rumen degradability of beef cattle. However, further research on effect of SBMFM on rumen ecology and production performance in meat and milk should be conducted using in vivo both digestion and feeding trials.
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    Effects of microbial fermented liquid (MFL) supplementation on gas production kinetics and digestibility using in vitro gas production technique
    (2018-12-01)
    Nampukdee, R.
    ;
    Polyorach, S.
    ;
    Wanapat, M.
    ;
    Kang, S.
    ;
    Cherdthong, A.
    The effects of microbial fermented liquid (MFL) supplementation on rumen fermentation and digestibility of dairy steer using in vitro gas production technique was studied. The two rumen fistulated dairy steers as a rumen fluid donor was used. The levels of source of microbes (yeast (Y) and microbial fermented liquid (MFL) were incorporated with the levels of supplementation (0, 10, 20 and 30 % of concentrate) was recorded. It was found that the intercept value (a) and IVDMD and IVOMD were interacted (p<0.01) between microbial source and supplement levels, while, supplement levels affected on a and the insoluble fraction (b), microbial source affected on b, potential extent of gas production (a+b) and cumulative gas production at 72 h and IVOMD. Moreover, supplementation of MFL with 20 % of concentrate were the highest (p<0.05) of b, c, a+b, cumulative gas production at 72 h, IVDMD and IVOMD. In conclusion, supplementation of MFL could improve nutritional digestibility and a possible productivity in ruminants.