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    In vitro rumen fermentation and methane production as affected by rambutan pee powder
    (2018-09-12)
    Gunun, Pongsatorn
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    Gunun, Nirawan
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    Cherdthong, Anusorn
    ;
    Wanapat, Metha
    ;
    The current study was aimed to determine the effect of rambutan peel powder (RPP) supplementation on in vitro gas production, rumen fermentation characteristics and methane production. The experimental design was a completely randomized design and the dietary treatments were different levels of RPP supplementation at 0, 4, 8, 12, 16 and 20 mg/0.5 g DM. Under this investigation, RPP supplementation did not affect gas production kinetics and in vitro digestibility (p >.05). The concentration of NH3-N decreased linearly with the increasing levels of RPP supplementation (p <.05). Propionate was increased (p <.05) when supplemented with RPP at 16 mg, while acetate and butyrate remained the same. On the other hand, supplementation of RPP decreased methane production (p <.05). This study indicated that RPP at 16 mg could be used as a rumen enhancer for manipulating rumen fermentation.
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    Improving the quality of sugarcane bagasse by urea and calcium hydroxide on gas production, degradability and rumen fermentation characteristics
    (2017-12-01)
    Gunun, N.
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    Gunun, P.
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    Wanapat, M.
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    Cherdthong, A.
    ;
    Kang, S.
    The objective of this study was to determine the influence of urea and whole soybean meal or calcium hydroxide [Ca(OH)<inf>2</inf>] treatment on sugarcane bagasse nutritive values and in vitro fermentation. The experimental design was a completely randomized design (CRD) and the dietary treatments were sugarcane bagasse treated with urea (0, 20, 40g/kg) and whole soybean meal or Ca(OH)<inf>2</inf> (0, 25, 50g/kg). It was found that the protein content of sugarcane bagasse was significantly increased by whole soybean meal and urea treatment, and the values ranged from 65-71 g/kg DM. On the other hand, treatment of Ca(OH)<inf>2</inf> 50 g/kg with urea 40 g/kg could reduce (p<0.05) fiber content of sugarcane bagasse and the values were 533, 514 and 104 g/kg DM for NDF, ADF and ADL, respectively. Gas production from the insoluble fraction (b), potential extent of gas production (a+b) and cumulative gas production were significantly increased (p<0.05) in sugarcane bagasse treated with urea 40 g/kg and urea 40 g/kg + whole soybean meal 25 g/kg (55.3, 53.5 and 50.1 ml), respectively. Urea and Ca(OH)<inf>2</inf> treatment could increase DM and OM degradability, true digestibility and microbial mass of sugarcane bagasse(p<0.05). Furthermore, the concentration of C3, C2 and C2:C3 were improved by urea and whole soybean meal or Ca(OH)<inf>2</inf> treatment. In addition, methane production was decreased in sugarcane bagasse treated with urea 40 g/kg in the present study. Based on this experiment, it could be concluded that treatment of urea and Ca(OH)<inf>2</inf> was an alternative method to improve the nutritive value of sugarcane bagasse, gas production, digestibility and ruminal fermentation characteristics.
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    Winged bean tuber as a novel alternative to corn meal in concentrate mixture in Thai native beef cattle diets
    (2025-12-01)
    Unnawong, Narirat
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    Cherdthong, Anusorn
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    Chankaew, Sompong
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    Rakvong, Teppratan
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    Suriyapha, Chaichana
    Background: In times of high feed costs and severe feed competition, it is crucial to explore alternative feedstuffs. Identifying alternative feed sources is essential for developing cost-effective and sustainable solutions to address challenges in livestock production and economic constraints. This study evaluates the effects of replacing corn with different levels of winged bean tuber (WBT) in concentrate mixtures on feed intake, feed utilization, and rumen fermentation in Thai native beef cattle. Animals were randomly assigned to a 4 × 4 Latin square design, with WBT replacing corn in the concentrate mixture at levels of 0, 33, 67, and 100%. Results: Replacing corn with WBT (0–100%) in the concentrate diet did not affect (p > 0.05) dry matter, organic matter, or crude protein intake. However, neutral detergent fiber (NDF) and acid detergent fiber (ADF) intake increased linearly (p < 0.05) at 67% and 100% replacement levels. Although nutrient digestibility remained unchanged, ruminal pH, ammonia-nitrogen concentration, and protozoal population increased linearly (p < 0.05) at 67% and 100% WBT replacement levels. However, WBT replacement did not significantly affect blood urea nitrogen (BUN) concentrations. As the level of WBT increased in the concentrate diet, the concentration of propionic acid (C3) increased, but the amount of acetic acid (C2) and the ratio of C2 to C3 decreased linearly (p < 0.05) without shifting the acetic concentration. Additionally, replacing corn with WBT in the concentrate diet had no effect on nitrogen balance. Conclusion: The WBT can be used as an alternative for corn grain up to 100% in ruminant diets without changing feed digestibility, the BUN, and nitrogen balance. It can also improve the fermentation characteristics in the rumen.
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    Effects of microbial fermented liquid (MFL) supplementation on gas production kinetics and digestibility using in vitro gas production technique
    (2018-12-01)
    Nampukdee, R.
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    ;
    Wanapat, M.
    ;
    Kang, S.
    ;
    Cherdthong, A.
    The effects of microbial fermented liquid (MFL) supplementation on rumen fermentation and digestibility of dairy steer using in vitro gas production technique was studied. The two rumen fistulated dairy steers as a rumen fluid donor was used. The levels of source of microbes (yeast (Y) and microbial fermented liquid (MFL) were incorporated with the levels of supplementation (0, 10, 20 and 30 % of concentrate) was recorded. It was found that the intercept value (a) and IVDMD and IVOMD were interacted (p<0.01) between microbial source and supplement levels, while, supplement levels affected on a and the insoluble fraction (b), microbial source affected on b, potential extent of gas production (a+b) and cumulative gas production at 72 h and IVOMD. Moreover, supplementation of MFL with 20 % of concentrate were the highest (p<0.05) of b, c, a+b, cumulative gas production at 72 h, IVDMD and IVOMD. In conclusion, supplementation of MFL could improve nutritional digestibility and a possible productivity in ruminants.
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    Effect of Delonix regia seed meal supplementation in Thai native beef cattle on feed intake, rumen fermentation characteristics and methane production
    (2017-10-01)
    Supapong, C.
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    Cherdthong, A.
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    Seankamsorn, A.
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    Khonkhaeng, B.
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    Wanapat, M.
    The aim of this research was to investigate the effect of supplementing Delonix regia (DR) seed meal on feed intake, digestibility, rumen fermentation, nitrogen balance and CH<inf>4</inf> production in Thai native beef cattle fed on rice straw. Four Thai native beef cattle with the initial body weight (BW) of 100 ± 5.0 kg were randomly assigned according to a 4 × 4 Latin square design to receive DR seed meal supplementation at 0, 90, 180 and 270 g/d. The present results revealed that the total intake (g/kg BW<sup>0.75</sup>) was significantly increased with the inclusion of dry matter (DM) seed meal at 270 g (P < 0.05). DM and OM digestibility were decreased when increasing DR seed meal levels (P < 0.05). Ruminal NH<inf>3</inf>-N concentration increased in beef cattle receiving DR seed meal. Supplementation of DR seed meal did not alter fungal zoospores’ concentration (P > 0.05), whereas the protozoal population was at 0, 4 h post feeding, and the mean values reduced when increasing the levels of DR seed meal supplemented (P < 0.05). The concentration of propionic acid at 4 h post feeding and its average concentration were significantly highest when 270 g DR seed meal was supplemented (P < 0.05). Estimation of CH<inf>4</inf> concentrations and CH<inf>4</inf> per dry matter intake were found reduced when increasing its DR seed meal concentration. In addition, N absorption, N retention and proportion of N retention to N intake were enhanced when 270 g DR seed meal was supplemented (P < 0.05). Thus, the inclusion of DR seed meal at 270 g/d resulted in improving total feed intake, rumen fermentation and N balance whereas there was reduced DM digestibility, protozoal population and CH<inf>4</inf> production in beef cattle fed rice straw base.