Choengchan, Nathawut

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Preferred name
Choengchan, Nathawut
Alternative Name
Choengchan, N.
Choengchan, Nuthawut
Main Affiliation
Email
nathawut.ch@kmitl.ac.th

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    Item type:Publication,
    Simple and fast fabrication of microfluidic paper-based analytical device by contact stamping for multiple-point standard addition assay: Application to direct analysis of urinary creatinine
    (2020-04-01)
    Mathaweesansurn, Arjnarong
    ;
    Thongrod, Suthathip
    ;
    Khongkaew, Putthiporn
    ;
    Phechkrajang, Chutima Matayatsuk
    ;
    Wilairat, Prapin
    In this work, a microfluidic paper-based analytical device (μPAD) for simultaneous multiple-point standard addition assay was fabricated by rubber stamping the hydrophobic barrier pattern onto a laboratory filter paper. The μPAD has a central zone from which an applied sample flows into eight surrounding narrow channels to which had been added the standard solutions. Each channel is connected to a circular area loaded with the reagent. The opposite end of this reagent zone is connected by second narrow channel to the final circular detection zone. The μPAD was applied to the measurement of creatinine in human urine. After addition of a urine sample, the orange-colored product arising from the Jaffé reaction is formed at the eight detection zones. A digital image of the μPAD is then recorded and the ratio of the red/green (R/G) intensity obtained using the ImageJ™ program is used in the quantitation of creatinine. The normal standard addition calibration line is constructed using the intensity ratio against the added creatinine concentrations (50–1000 mg L<sup>−1</sup>). Good linearity was achieved (r<sup>2</sup> ˃ 0.99). There were no significant differences between the creatinine content using an HPLC method (paired t-test at 95% confidence, t<inf>stat</inf> = 1.78, t<inf>critical</inf> = 2.26, n = 10). The use of simultaneous multiple-point standard addition calibration allows rapid determination of creatinine in urine with elimination of matrix interference.
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    Item type:Publication,
    Microfluidic paper-based analytical device for determination of sucrose in sugarcane juice using Benedict’s reagent
    (2022-11-01)
    Ratanawimarnwong, Nuanlaor
    ;
    Suksomphot, Vanlada
    ;
    Sornpipatpong, Khemika
    ;
    Lengwan, Supamit
    ;
    Donpudsa, Suchao
    This work presents a microfluidic paper-based analytical device (μPAD) for the determination of sucrose using the Benedict’s test. An asymmetric dumbbell-shaped hydrophobic barrier was produced by rubber stamping the barrier pattern onto a laboratory filter paper. Hydrochloric acid and solution containing sucrose were successively deposited onto the sample reservoir of the μPAD attached to a glass slide. The device was placed in a plastic bag and dipped into boiling water for accelerating the hydrolysis of sucrose into the reducing sugars. Then the Benedict’s reagent was added at the narrow straight channel connecting the two circular zones of the μPAD, which was replaced in the plastic bag and heated again for reduction of Cu(II) by the reducing sugars. Precipitate of brick-red copper(I) oxide was formed. The image of the μPAD was recorded by a smartphone. The ratio of the red to blue intensities gave linear correlation with the concentration of sucrose in the range of 0.5–10% w/v. The relative standard deviation of the measurement was less than 5% for 2 and 4% w/v sucrose (n = 10), with limit of determination, calculated using standard deviation of regression divided by slope of calibration, of 0.26% w/v sucrose. The method was successfully validated using the dinitrosalicylic acid method for sucrose measurement. Percent recoveries of sucrose were evaluated using ten sugarcane samples. The recoveries were in the range of 89 to 101%, demonstrating that there were no significant sample matrix effects on the quantification.