Chutipaijit, Sutee

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Chutipaijit, Sutee
Alternative Name
Chutipaijit, S.
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Email
sutee.ch@kmitl.ac.th

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    In vitro plant growth promotion by ZnO nanomaterials in indica rice seedlings (Oryza sativa L.)
    (2018-01-01)
    Chutipaijit, Sutee
    ;
    Whalley, Anthony J.S.
    ;
    Sutjaritvorakul, Thanawat
    This research focuses on zinc oxide nanomaterials (nano-ZnO) applied to agricultural fields. Plants were exposed to nanomaterials and responded differently in tolerance to the various concentrations and types of nanomaterials. Two cultivars (SP1 and SP90) of indica rice (Oryza sativa L.) seedlings were grown at 0-1,000 mg.L<sup>-1</sup> nano-ZnO and their morphological and physiological response was determined. Rice seedlings were harvested 5, 7, 10 and 14 days after treatments. Results demonstrated a significant increase in biomass, growth tolerance index (GTI), chlorophyll contents and antioxidant enzyme activities of the treated plant at 100 mg.L<sup>-1</sup> nano-ZnO but these parameters decreased with increasing nano-ZnO concentration in the treated plants at 300-1,000 mg.L<sup>-1</sup> nano-ZnO when compared to control plants (0 mg.L<sup>-1</sup> nano-ZnO). The high accumulations of biomass, GTI and chlorophyll contents were related to production of antioxidant enzyme activities (catalase and peroxidase) in plant cells when plants were exposed to a low dose of nano-ZnO. Further investigations will determine the effect of nano-ZnO on the plant productivity.
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    Antioxidant Properties derived from Stromata of Xylaria species collected from Tropical Forest in Thailand
    (2015-03-01)
    Orachaipunlap, Krittapong
    ;
    Suwannasai, Nuttika
    ;
    Chutipaijit, Sutee
    ;
    Whalley, Anthony J.S.
    ;
    Sihanonth, Prakitsin
    Fungi in the genus Xylaria are common wood decay fungi. They have been shown to be potential sources of novel natural products for utilization in medicine, agriculture and industry. In this study, stromata of Xylaria spp. were collected from Trat Agroforestry Research and Training Station. Fresh specimens of Xylaria spp. were cultured on Potato Dextrose Agar (PDA) for 14 days and then transferred to Malt Extract Broth (MEB). All isolates were cultured under static condition for 6 weeks and extracted with ethyl acetate. Antioxidant properties of stromatic Xylaria spp. were determined in vitro by ABTS and DPPH assays. Free radical scavenging was recorded by Trolox equivalent antioxidant capacity (TEAC) in ABTS assay and EC<inf>50</inf> in DPPH assay. The ethyl acetate extracts of four stromatic Xylaria spp. (TR25, TR34, TR46 and TR51) showed strong activities against both ABTS (TEAC 20.7±0.08, 15.16+0.03, 11 ±0.03 and 19.66±0.11 μM Trolox respectively) and DPPH assay (EC<inf>50</inf> 36.92±0.96, 70.53±1.04, 102±2.67 and 49.08 ±1.74 μL respectively). The correlation coefficients from regression analysis showed a positive correlation between total phenolic/flavonoid contents and TEAC for ABTS assay of all crudes extracted and showed a negative correlation between total phenolic/flavonoid contents and the EC<inf>50</inf> for DPPH radical scavenging. Moreover, GC/MS results are indicated the various phenolic compounds in selected crude extracts. In conclusion, cultures obtained from stromata of Xylaria spp. may have potential as natural antioxidant products.
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    Enzymatic degradation of modified gelatin and carboxymethyl cellulose scaffolds
    (2018-01-01)
    Sutjaritvorakul, Thanawat
    ;
    Wiwatwongwana, Fasai
    ;
    Imsuwan, Pattareewan
    ;
    Whalley, Anthony J.S.
    ;
    Chutipaijit, Sutee
    The modification of biological and synthetic biopolymer provides suitable properties for bone and tissue engineering. Modified gelatin and carboxymethyl cellulose (CMC) scaffolds are interesting biomaterials for applying as artificial dermal skin. The objective of this research was to investigate the enzymatic degradation of gelatin and carboxymethyl cellulose scaffolds. The samples were prepared in different compositions of gelatin and CMC (Gelatin:CMC) (w/w) such as 9:1, 8:2, 7:3 and 6:4. The enzymatic degradation test was undertaken using cellulase produced by Aspergillus niger. The results demonstrated that GC64 (Gelatin:CMC, 6:4) exhibited the highest degree of enzymatic degradation and the behavior of the degradation was predicated based on water absorption ability. Moreover, the morphology of degraded residues were investigated by scanning electron microscopy (SEM).