Chutipaijit, Sutee

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Preferred name
Chutipaijit, Sutee
Alternative Name
Chutipaijit, S.
Main Affiliation
Email
sutee.ch@kmitl.ac.th

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    Item type:Publication,
    Enhancements of growth and metabolites of indica rice callus (Oryza sativa l. cv. pathumthani1) using tio2 nanoparticles (nano-tio2)
    (2020-04-01)
    Chutipaijit, S.
    ;
    Sutjaritvorakul, T.
    In this research, the effects of a various in concentration of TiO<inf>2</inf> nanoparticles (Nano-TiO<inf>2</inf>) were applied to study the cell growth, metabolites and antioxidant responses of callus in indica rice plants. Dose-dependent changes in growth performances, metabolite accumulations and antioxidant activities of callus were found. Mature seeds of indica rice cv. Pathumthani1 were cultured in callus induction medium with different concentrations of Nano-TiO<inf>2</inf> (0-600 mg L<sup>-1</sup>). After five weeks of cultivation, the callus formations were measured in fresh weights and dry weights, and the contents of phenolic compounds, flavonoids, and the antioxidant activities of callus extracts were assayed. The Nano-TiO<inf>2</inf> induced of fresh weights and dry weights in callus formations when compared with the control treatment (0 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>) were also observed. The flavonoid and phenolic compound contents in rice callus exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf> were 118% and 139%, respectively, higher than rice callus unexposed to Nano-TiO<inf>2</inf>. Moreover, the antioxidant activities of the callus metabolites studied shows that the percentages of inhibition were up-regulated (84%) when exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>, as a response to the high-level depletion of the free radicals in metabolites of rice callus. The results obtained from this research showed that the addition of Nano-TiO<inf>2</inf> had impacts on the callus growth and metabolite accumulations in rice callus. The results suggested that addition of Nano-TiO<inf>2</inf> at appropriate levels could role as an elicitor for biosynthesis of valuable metabolites and antioxidant properties for pharmaceutical applications in further research.
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    Physiological and metabolic modifications in response to nanocarbon in callus of Indica rice cultivar
    (2020-01-01)
    Sutjaritvorakul, Thanawat
    ;
    Chutipaijit, Sutee
    Nanocarbon has been shown to be implicated in the response of plants to an assortment of positive or negative impacts. In the present research, the effects of nanocarbon on cell growth and secondary metabolite production on rice callus were studied. Different concentrations of nanocarbon (0-1000 mgl<sup>−1</sup>) were added into callus induction media to investigate the effects on the growth of callus, and secondary metabolite production in indica rice callus (Oryza sativa L. cv. Pathumthani1). The growth of callus was measured in terms of the size and weight of callus, and secondary metabolite production was determined based on the levels of total phenolic compounds and flavonoid production. The results showed that the callus induction, the callus growth and the secondary metabolite production in rice callus after treatment with nanocarbon were significantly different when compared to control (without treatment with nanocarbon). The rice callus treated with low (100 and 200 mgl<sup>-1</sup>) and moderate (400 and 600 mgl<sup>-1</sup>) level of nanocarbon displayed an enhancement in callus growth, total phenolic compounds and flavonoid production. However, the induction of callus, cell growth, and secondary metabolite production were decreased when rice callus was treated with high concentrations of nanocarbon (800 and 1000 mgl<sup>-1</sup>). The results of this research showed that the nanocarbon application of 400-600 mgl<sup>-1</sup> had potential to induce the cell growth and secondary metabolite production. This research suggests further investigation in using different concentrations of nanocarbon on other plant species for potential pharmaceutical application.