Parinthawong, Nonglak

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Preferred name
Parinthawong, Nonglak
Alternative Name
Parinthawong, N.
Main Affiliation
Email
nonglak.pa@kmitl.ac.th

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    Item type:Publication,
    Development of novel InDel markers by whole-genome sequence comparison and genetic diversity assessment of Thailand rice blast fungus populations
    (2025-12-01)
    Thamkirati, Napassorn
    ;
    Suktrakul, Worrawit
    ;
    Ngernmuen, Athipat
    ;
    Toojinda, Theerayut
    ;
    Katengam, Sureeporn
    InDel markers are commonly used to assess genetic relationships among populations. In this study, we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyricularia oryzae. We analyzed 152 whole-genome sequences of P. oryzae isolates from diverse global regions, including Brazil, Burundi, China, Colombia, Côte d'Ivoire, France, Ghana, Hungary, India, Japan, Korea, Laos, Madagascar, Mali, Morocco, Nepal, the Philippines, Portugal, Spain, Suriname, Thailand, the UK, the USA, and Zambia. Our analysis identified a total of 233,595 InDel loci distributed across the seven chromosomes of P. oryzae. From these, 82 loci were selected based on their high polymorphism across the 152 genome sequences. The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates, GUY11 (France) and KJ201 (Korea). Of the 82 InDel loci, 33 exhibited polymorphisms, with 2–4 alleles per locus and polymorphic information content (PIC) scores ranging from 0.04 to 0.67. Principal coordinate and structure analyses revealed two genetic subgroups among the Thai rice blast isolates, categorized according to host specificity. Genetic relationships highlighted disparities among rice blast populations based on their respective hosts: rice and grassy weeds. This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease. The newly developed InDel markers provide a valuable resource for future research in this field.
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    Item type:Publication,
    Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae
    (2022-09-01)
    Pradapphai, P.
    ;
    Kumlung, T.
    ;
    Parinthawong, N.
    Pyricularia oryzae causes rice blast disease and is one of the most significant plant pathogens worldwide. The rice blast fungus can produce a variety of phytotoxic secondary metabolites and has been found to be essential for host invasion. Two isolates, virulent isolate RBR55003 and non-virulent isolate CCO56003 were selected which based on their abilities to cause blast symptoms on rice leaves. Pyriculol and picolinic acid were detected in culture filtrate of both isolates during the exponential growth phase. Expression of genes involved in fungal pathogenicity and plant defense were analyzed. The expression of MoPks19 gene in isolate RBR55003 was higher than avirulent isolate CCO56003, indicating that MoPks19 may play a role in the pathogenesis of RBR55003. The expression of the OsWRKY30 gene that plays an important role in the regulation of a defense-related gene in rice was higher in CCO56003 inoculated than in RBR55003 inoculated rice sample, indicating the increased resistance of rice plants occurring in rice after non-virulent isolate infection, while the expression of the OsCPS4 gene involved in phytoalexin synthesis in rice was not different after isolate infections. Gene expression analysis revealed different responses according to plant-pathogen relationships.
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    Item type:Publication,
    Genetic mapping of leaf blast resistance gene in landrace rice cultivar ‘GS19769’
    (2015-08-31)
    Parinthawong, Nonglak
    ;
    Tansian, Pennapar
    ;
    Sreewongchai, Tanee
    The rice blast fungus, Magnaporthe oryzae, is highly varied and therefore overcomes resistance in a few years. Rice cultivars with the ability to resist different blast races are therefore required. In this study, a resistance gene of landrace rice cultivar ‘GS19769’ is identified using a mix of 19 blast isolates collected from several epidemic areas in Thailand. The selected cultivar was fertilised with the blast susceptible variety, Khao Dok Mali 105 (KDML105), to generate the mapping population. Segregation analysis in the F<inf>2</inf> population shows that ‘GS19769’ contains more than 1 resistance gene, as the Chi-square test for segregation of resistance and susceptibility does not fit the ratio of 3:1. The bulk segregant analysis by simple sequence repeat (SSR) markers shows that the identified resistance gene is linked to the SSR markers RM224 and RM144 on chromosome 11. Analysis of 15 F<inf>2</inf> plants susceptible to the blast was conducted. The gene was mapped to RM224 and RM144 at the same distance of 20 cM.