Parinthawong, Nonglak
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Parinthawong, Nonglak
Alternative Name
Parinthawong, N.
Main Affiliation
Email
nonglak.pa@kmitl.ac.th
26 results
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Item type:Publication, Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)(2015-11-01); ; ; An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Development of novel InDel markers by whole-genome sequence comparison and genetic diversity assessment of Thailand rice blast fungus populations(2025-12-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation and expression analysis of the Flavanone 3-Hydroxylase genes in Lotus (Nelumbo nucifera gaertn.), Waterlily (Nymphaea sp.) and transient silencing in Waterlily(2017-04-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Tissue culture and transformation of the antisense DFR gene into lotus (Nelumbo nucifera Gaertn.) through particle bombardment(2013-09-24); ; Arunyanart, SumayThe effects of explant and plant growth regulators on callus induction and plant regeneration and particle bombardment-induced transformation were examined in lotus (Nelumbo nucifera Gaertn.) cv. Buntharik. The apical buds from embryos (~3. mm) formed an embryogenic callus when cultured on MS medium supplemented with 40. μM 1-napthaleneactic acid (NAA) and 0.5. μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) for 8 weeks. All of the embryogenic calluses were transferred to MS medium supplemented with 0, 40, 50 and 60. μM 6-benzyladenine (BA) and the highest number of shoots was achieved in the medium supplemented with 50. μM BA after 8 weeks of culture. The transformation of lotus using a particle bombardment device was examined with the shoot clusters from embryogenic apices. The pCAMBIA2301anti-DFR plasmid contained β. -glucuronidase (GUS) as a reporter gene, neomycin phosphotransferase (NPTII) as a selectable marker, and antisense dihydroflavonol 4-reductase (anti-. DFR) as the gene of interest. The particle bombardment procedure with a helium gas pressure of 1100. psi and a target distance of 6. cm yielded the highest number of blue spots (4.8 spots/shoot cluster). However, stable transformations, as confirmed by polymerase chain reaction and reverse transcription polymerase chain reaction analysis, were only achieved using a helium gas pressure of 1100. psi and a target distance of 9. cm. Our work illustrates that the stable transformation of lotus is possible, and we suggest that this methodology can be used to target and modify specific charactics of this plant. © 2013 Elsevier B.V. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae(2022-09-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Morphological and Molecular Identification of Pythium spp. from Hydroponically-Grown Lettuce(2022-01-01); Jaenaksorn, TanimnunHerein, hydroponic Pythium communities in Thailand were investigated. The 38 Pythium isolates from asymptomatic and symptomatic lettuce roots were identified using morphological and molecular features. The data indicated that P. aphanidermatum and P. myriotylum were the predominant species. Regarding the rDNA-ITS study, all isolates were identified as P. aphanidermatum, P. myriotylum, P. deliense, or an unidentified Pythium species. The reconfirmation of the three unidentified Pythium isolates using the cytochrome oxidase subunit I (COI) gene in comparison to the representative isolates was achieved using P. aphanidermatum and P. myriotylum. The COI phylogenetic trees were similar to that of the ITS tree. Additionally, pathogenicity of the Pythium representative isolates to plant seeds was evaluated in a laboratory assay. The seedlings suffered serious symptoms from P. myriotylum SR31 infection, with 90-100% disease severity. The other isolates presented disease severity of less than 20% when compared with uninoculated control. This study provides a comprehensive identification of Pythium root rot in lettuce grown on hydroponics in Thailand and provides information on beneficial microorganisms and a resistance inducer in lettuce root rot. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Mating type and genetic diversity analysis of Pyricularia oryzae collected from Thai rice varieties(2018-12-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Blast resistance evaluation and genetic inheritance of gene controlling leaf blast resistance in Dawk Pa-yawm Rai variety (GS23774)(2020-01-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Micromonospora oryzae sp. Nov., isolated from roots of upland rice(2015-11-01); ; ; An actinomycete strain, designated CP2R9-1<sup>T</sup>, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1<sup>T</sup> was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>) and MK-10(H<inf>4</inf>). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1<sup>T</sup> was closely related to Micromonospora haikouensis 232617<sup>T</sup> (99.32% similarity), Micromonospora carbonacea DSM 43168<sup>T</sup> (99.18%) and Micromonospora krabiensis MA-2<sup>T</sup> (99.16%). Strain CP2R9-1<sup>T</sup> was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3¡0.1– 41.7¡0.7%) and phenotypic differences. The results presented in this study showed that strain CP2R9-1<sup>T</sup> represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1<sup>T</sup> (5BCC 67266T5NBRC 110007<sup>T</sup>). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponics and its in vitro inhibitory effect on plant pathogenic fungi(2017-09-01); Jaenaksorn, TanimnunA series of experiments were conducted to investigate phytoalexin production from five varieties of lettuce in hydroponics using abiotic (2.5, 5% CuSO<inf>4</inf>; 0.5, 1% AgNO<inf>3</inf>) and biotic elicitors (non-pathogenic Pythium sp.) at different plant ages. It showed that phytoalexin was successfully induced in tested lettuce grown in hydroponics after elicitation with abiotic elicitors throughout the trial. Phytoalexin showed yellow fluorescent spot under 365 nm UV light with R<inf>f</inf> 0.45-0.48 and clear inhibition zone where Aspergillus niger failed to develop on TLC plate. For biotic elicitors, no yellow fluorescent spot on TLC plate was observed from tested lettuce varieties however inhibition zone at R<inf>f</inf> 0.9 was detected at 8-9 weeks from red oak, green oak, and red coral. Moreover, crude extract of lettuce elicited with abiotic elicitors possessed in vitro antifungal activity against C. gloeosporioides, C. lunata, F. oxysporum, and P. aphanidermatum probably due to phytoalexin (lettucenin A) in the extract.