Tangthirasunun, Narumon

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Tangthirasunun, Narumon
Alternative Name
Tangthirasunun, N.
Main Affiliation
Email
narumon.ta@kmitl.ac.th

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    Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan
    (2024-01-01)
    Hsu, Sheng Yu
    ;
    Xu, Yuan Cheng
    ;
    Lin, Yu Chen
    ;
    Chuang, Wei Yu
    ;
    Lin, Shiou Ruei
    Pestalotiopsis sensu lato, commonly referred to as pestalotiopsis-like fungi, exhibit a broad distribution and are frequently found as endophytes, saprobes and pathogens across various plant hosts. The taxa within pestalotiopsis-like fungi are classified into three genera viz. Pestalotiopsis, Pseudopestalotiopsis and Neopestalotiopsis, based on the conidial colour of their median cells and multi-locus molecular phylogenies. In the course of a biodiversity investigation focusing on pestalotiopsis-like fungi, a total of 12 fungal strains were identified. These strains were found to be associated with stromata of Beauveria, Ophiocordyceps and Tolypocladium in various regions of Taiwan from 2018 to 2021. These strains were evaluated morphologically and multi-locus phylogenetic analyses of the ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α) and tub2 (beta-tubulin) gene regions were conducted for genotyping. The results revealed seven well-classified taxa and one tentative clade in Pestalotiopsis and Neopestalotiopsis. One novel species, Pestalotiopsis manyueyuanani and four new records, N. camelliae-oleiferae, N. haikouensis, P. chamaeropis and P. hispanica, were reported for the first time in Taiwan. In addition, P. formosana and an unclassified strain of Neopestalotiopsis were identified, based on similarities of phylogeny and morphology. However, the data obtained in the present study suggest that the currently recommended loci for species delimitation of pestalotiopsis-like fungi do not deliver reliable or adequate resolution of tree topologies. The in-vitro mycelial growth rates of selected strains from these taxa had an optimum temperature of 25 °C, but growth ceased at 5 °C and 35 °C, while all the strains grew faster under alkaline than acidic or neutral pH conditions. This study provides the first assessment of pestalotiopsis-like fungi, associated with entomopathogenic taxa.
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    Diversity of Sordariales Fungi: Identification of Seven New Species of Naviculisporaceae Through Morphological Analyses and Genome Sequencing
    (2025-12-01)
    Tangthirasunun, Narumon
    ;
    Gautier, Valérie
    ;
    Lalanne, Christophe
    ;
    Bonometti, Lucas
    ;
    Cros-Arteil, Sandrine
    Thanks to next-generation sequencing (NGS) technologies, the diversity of fungi can now be investigated through the analysis of their genome sequences. Naviculisporaceae is a family within the Sordariales, whose diversity is not well-known, with only one genome sequence published for this family. Here, we report on the isolation and cultivation of 20 new strains of Naviculisporaceae. Their genome sequences, as well as those of the five commercially available strains, were determined, thus providing complete genome sequences for 25 new Naviculisporaceae strains. Species delimitation was conducted using a combination of (1) ITS + LSU phylogenetic analysis of the new isolates along with other known species of the family, (2) comparisons between DNA barcode sequences of the new strains with those of the known species, and (3) average genome-wide nucleotide identity calculation. We built a phylogenomic tree and studied the organization of the mating-type locus. In vitro fruiting was obtained for 16 strains, enabling the definition of seven new species, namely Pseudorhypophila gallica, Pseudorhypophila guyanensis Rhypophila alpibus, Rhypophila brasiliensis, Rhypophila camarguensis, Rhypophila reunionensis and Rhypophila thailandica, as well as two new combinations, namely Pseudorhypophila latipes and Pseudorhypophila oryzae. Eight strains for which in vitro fruiting was not obtained may belong to additional new species. These results expand the known diversity of the Naviculisporaceae and greatly enlarge the genomic data available for the family.
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    The Podospora anserina (Rabenh.) Niessl Species Complex in Metropolitan and Overseas France with Description of a New Species, Podospora reunionensis Silar, sp. nov.
    (2025-12-16)
    Silar, Philippe
    ;
    Gautier, Valérie
    ;
    Lalanne, Christophe
    ;
    Tangthirasunun, Narumon
    ;
    Arthur, Marvyn
    Podospora anserina (Rabenh.) Niessl is a model fungus that was shown to belong to a complex of seven species with morphologically indistinguishable sexual fruiting bodies. Through the morphological analyses and ITS (Intergenic Transcribed Spacer) sequences of 86 newly-isolated strains, as well as the genome sequences of five strains, we show that members of the P. anserina species complex present different distribution ranges in metropolitan and overseas France. Podospora anserina is present all-over metropolitan France, including Corsica, while P. comata Milovtz. is restricted to the Northern part and P. pauciseta (Ces.) Traverso to the Southern part of continental France. The Guadeloupe hosts P. pseudoanserina C.Boucher, T.S.Nguyen & P.Silar and La Réunion island a species new to science, described here as Podospora reunionensis Silar, sp. nov. This species is closely related to P. comata, but exhibit clear morphological and biological differences.