Rungjindamai, Nattawut
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Preferred name
Rungjindamai, Nattawut
Alternative Name
Rungjindamai, N.
Main Affiliation
Email
nattawut.ru@kmitl.ac.th
13 results
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Item type:Publication, Bacterial cellulose production and application on a fat replacer on fat-reduced Chinese sausage(2023-05-01) ;Oonnom, P.; Bacterial cellulose (BC) produced by Acetobacter xylinum TISTR 976 strain was used as a fat replacer in Chinese sausage and the properties of this product were investigated in the present study. The BC was prepared by fermentation with a coconut medium for 10 days. To remove bacterial cells, the purified BC was soaked in 1% (w/v) NaOH. The bacterial cellulose powder (BCP) was prepared by two methods including soaking in NaOH (BCP-M1) and grinding and soaking in NaOH (BCP-M2). The BC was neutralized, squeezed and kept in -20°C. The frozen BC were freeze dried at -50°C for 8 h. The BC dried product was finally ground into fine powder (diameter 0.5 mm). The second method (BCP-M2) produced the better quality of BC by showing physical appearance, water holding capacity, oil holding capacity were not significant difference from commercial BCP. In addition, BCP-M2 presented non-cytotoxic effect to Vero cell by MTT cytotoxicity. BCP can be replaced for pork fat for Chinese sausage production. The fat contents of Chinese sausage were reduced significantly (1-2%) when BCP was incorporated into the Chinese sausage. Fiber contents were increased significantly in this product compared to those of control (containing no BCP and 24% fat). 1% BCP treatment and control treatment had the higher springiness scores than other samples. Textural hardness was significantly increased for BCP-added treatment. Moreover, the concentration of 1% BCP also increased acceptable sensory qualities. The product shelf life in the vacuum-package was studied at 4°C and room temperature (30±2°C) for 28 days. Thiobarbituric acid value was increased at room temperature storage. When it was stored at 4°C, there is a few changes of concentration of thiobarbituric acid. The result indicated that BCP could be used to replace fat in the production of Chinese sausage. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Efficacy of biopesticides and fungicides against brown rot on cherry (Prunus avium) and plum (Prunus domestica)(2025-01-01); ;Jeffries, PeterXu, XiangmingCherry and plum are prone to brown rot diseases. Synthetic fungicides are still the main management tool to control brown rot but there is a need to develop eco-friendly methods. Five commercial biopesticides including BioPK (Trichoderma viride, T. harzianum and T. longibranchum), BoniProtect (Aureobasidium pullulans), Trianum (T. harzianum), PreStop (Gliocladium catenulatum) and Serenade (Bacillus subtilis) along with Indar (fenbuconazole, a synthetic fungicide) were tested against Monilinia laxa which products applied before and after pathogen inoculation. Indar completely inhibited brown rot development. Serenade partially inhibited M. laxa when applied before pathogen inoculation. The efficacy of two indigenous strains (B. subtilis B91 and A. pullulans Y126) were evaluated under ambient conditions or 1°C; Indar and Serenade were used as control treatments. Cherry and plum were first treated with specific products, then wounded and inoculated with M. laxa. Indar completely inhibited M. laxa under both conditions. Under ambient conditions, only B91 reduced the incidence of disease. When stored at 1°C for 4 weeks, all biocontrol treatments reduced the incidence of disease. However, when fruits were left under ambient conditions for an additional 1 week, the incidence of disease did not differ between treatments. Post-harvest application of biopesticides under 1°C was studied with Rovral used as a control. Cherry and plum were submerged into individual treatments and stored at 1°C for 6 weeks. Rovral reduced the incidence of disease, whilst all biopesticides did not. In conclusion, Serenade, B91 and Y126 can partially inhibit M. laxa when applied before pathogen infection. The present study supports that these biocontrol proeducts should be used pre-harvest to reduce the extent of fruit latent infection by M. laxa, and an effective post-harvest cold chain management should be implemented to delay/minimise rot development. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Unravelling the hidden diversity of cave mycobiota in Thailand’s Satun Geopark(2023-12-01) ;Suetrong, Satinee ;Preedanon, Sita ;Kobmoo, Noppol ;Srihom, CharisaSomrithipol, SayanhKarst caves are distinctive ecosystems that have limited nutrients, darkness, low to moderate temperatures, and high moisture levels, which allow for a diverse range of fungal communities to thrive. Despite their significance, little is understood about the fungi found in karst caves in Thailand. In 2019, we studied the cultured mycobiota from five substrate types (air, water, rock, soil/sediment, and organic debris) in two karst caves (Le Stegodon and Phu Pha Phet Caves) of the Satun UNESCO Global Geopark, southern Thailand. A cumulative count of 829 distinct fungal morphological types was identified, encompassing 319 fungal culturable were observed. Based on preliminary analyses of the internal transcribed spacer (ITS) sequence using BLAST searches, the most common phylum among the fungal morphotypes was Ascomycota, harboring 282 species in 91 genera, 93.4% of which were distributed in the classes Eurotiomycetes, Sordariomycetes, and Dothideomycetes. The most common fungal genera identified in the two karst caves were Aspergillus, Penicillium, Cladosporium, Talaromyces, Xylaria, and Trichoderma, with 45, 41, 24, 14, 14, and 6 species identified, respectively. Discovering fungi in Thai karst caves highlights the extensive fungal diversity in the Satun UNESCO Global Geopark, implying undiscovered species, and emphasizing the need for comprehensive investigations in other unexplored Thai karst caves. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Utilization of Agricultural Waste for the Cultivation of Pleurotus Mushrooms in Thailand(2024-06-01); ;Trakunjarunkit, Kittipong ;Posalee, ThanutchaLimpanya, DuangkamolThailand is one of the major agricultural production countries in the world. Therefore, large amounts of agricultural waste are generated as by-products of the agroindustry. The wastes are usually discarded or burnt, resulting in environmental pollution. The main goal of this research was to evaluate the use of agricultural waste for mushroom cultivation. A total of 15 mushroom isolates were recovered from mother spawns and basidiocarps. They were screened for their cellulolytic enzyme activity on Czapek agar using carboxymethyl cellulose (CMC) as the sole carbon source. Two isolates of the oyster mushrooms, Pleurotus pulmonarius PP6 and Pleurotus ostreatus PO3, produced the best enzyme activities. To observe the mycelial growth on agricultural waste, the two oyster mushroom candidates were cultured in jars containing five different types of agricultural waste: corn husk, rice straw, coconut meal, coconut husk and sugarcane bagasse, and the jars were incubated at 25°C for six weeks. The results show that both isolates grew best on coconut meal, producing very densely packed mycelia. Meanwhile, corn husk and rice straw were also good sources for oyster mushroom cultivation. This study shows that these three substrates have the potential to be utilized in mushroom cultivation on a commercial scale. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation and identification of fungi with glucoamylase activity from loog-pang-khao-mak (A Thai Traditional Fermentation Starter)(2020-03-01) ;Roongrojmongkhon, Nawaphorn; ; Loog-pang-khao-mak is a Thai traditional fermentation starter that has been used for production of Thai fermented foods for decades. This research aimed to isolate and identify the fungi that produce effective glucoamylase but low ethanol content from the starter. A total of 166 isolates were screened from twelve samples of Loog-pang-khao-mak accumulated from 12 provinces in Thailand using dichloran rose bengal chloramphenicol agar (DRBC Agar). Seventy-nine isolates that effectively hydrolyze starch were selected for glucoamylase activity and alcohol production assay. Three yeast isolates exhibited high glucoamylase activity ranging from 139.14 to 140.94 unit/ml and lowest alcohol yield of 0.41% (v/v) were Saccharomycopsis fibuligera using morphological and molecular identification. The five isolates of mold exhibited high glucoamylase activity (149.20 to 152.60 unit/ml) were identified as Aspergillus Niger, Aspergillus oryzae and Amylomyces rouxii. These findings provide further knowledge on the fungi and their potential use as traditional inocula for fermentation of food products. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A Study of Oyster Mushrooms for Producing Cellulase Enzyme(2022-09-01) ;Numsiri, Jirapornpong ;Khithen, Teerawat ;Sukkasem, ApiwatThe objective of this research was to study three species of Pleurotus spp., are widely consumed edible mushrooms, consisting of P. sajor-caju, P. ostreatus and P. cystidiosus. Basidiocarp and spawn on sorghum seeds were collected from seven provinces in Central and Eastern of Thailand. Thirteen isolates were recovered from 15 samples which include P. sajor-caju, P. ostreatus and P. cystidiosus for 5, 6 and 2 isolates, respectively. The studies of their growth rates and enzymatic capability were performed on potato dextrose ager (PDA) and Czapek Dox Agar mixed with 1% carboxymethyl cellulose (CZ+CMC), respectively. Both media were incubated at 25ºC for seven days. The three most potential isolates were selected including P. sajor-caju PS5, P. ostreatus PO7 and P. cystidiosus PC1. Therefore, these mushrooms have potential for development in the future. Because they can grow rapidly and produce cellulase effectively. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characteristics and Antioxidant Activities of Kombucha from Black Tea and Roselle by a Mixed Starter Culture(2023-12-29) ;Sutthiphatkul, Tanyarat ;Mangmool, Supachoke; Kombucha is a functional beverage fermented by the symbiosis of bacteria and yeasts and is comprised mainly of bioactive compounds and acids that have beneficial health effects. Our research aimed to determine the optimal ratio of co-culture of the bacterium Acetobacter pasteurianus AJ605 and the yeast Zygosaccharomyces bailii YN403 for kombucha fermentation in order to improve the consistency of kombucha quality so that it can be mass produced. Microbial growth, chemical characteristics, and antioxidant activities of kombucha during fermentation were analyzed. The results showed that a co-culture ratio of 8:2 (v/v) of A. pasteurianus AJ605 and Z. bailii YN403 gave the highest antioxidant activity after 10 days of fermentation, with a DPPH IC<inf>50</inf> value of 25.76 µl/mL and an ABTS IC<inf>50</inf> value of 8.84 µl/mL. The co-culture ratio of 8:2 showed antioxidant activity that was not significantly different (p>0.05) from that of SCOBY (symbiotic culture of bacteria and yeasts) alone. Additionally, during fermentation, the pH of kombucha decreased to 3.16, with increases in titratable acidity and alcohol content of 7.00 g/L and 7.96 g/L, respectively. To enhance the antioxidant activity and taste quality of kombucha, black tea and roselle were mixed at various ratios and inoculated with 10% (v/v) optimal co-culture. The results showed that a high antioxidant activity was obtained using an 8:2 (w/w) ratio of black tea and roselle, at day 10 of fermentation, with a DPPH IC<inf>50</inf> value of 23.88 µl/mL and ABTS IC<inf>50</inf> value of 6.11 µl/mL. Moreover, the results indicate that kombucha from black tea and roselle can be a functional beverage that has high antioxidant activity and sensory acceptability. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Ophiocordyceps asiana and Ophiocordyceps tessaratomidarum (Ophiocordycipitaceae, Hypocreales), two new species on stink bugs from Thailand(2021-03-01) ;Khao-ngam, Srikhun ;Mongkolsamrit, Suchada; ;Noisripoom, WasanaPooissarakul, WuttikornTwo new Ophiocordyceps species from Thailand, which belong to Ophiocordyceps nutans species complex and attack adult and nymph of stink bugs, are described: Ophiocordyceps asiana and O. tessaratomidarum. Molecular phylogenetic analyses of ITS and a combined sequence data of LSU, RPB1 and TEF1 revealed that these two new species are distinct from other known species in Ophiocordyceps nutans species complex. Based on the findings on morphological studies, they further support the naming of these two novel species. Ophiocordyceps asiana is parasitic on adult and nymph stink bugs, members of Coreidae and Pentatomidae which were recorded from China, Japan and Thailand, while O. tessaratomidarum is reported from Thailand for the first time and is only found as a parasite on adult stink bug, Tessaratoma papillosa (Tessaratomidae, Hemiptera). The fertile heads of O. asiana are shorter than reported for O. tessaratomidarum, and the colours of fertile heads producing teleomorph of these two species differs from each other by having strong reddish orange or light yellowish pink in O. asiana and pale yellow in O. tessaratomidarum. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review(2024-01-01); Jones, E. B.GarethA review of selected studies on fungal endophytes confirms the paucity of Basidiomycota and basal fungi, with almost 90% attributed to Ascomycota. Reasons for the low number of Basidiomycota and basal fungi, including the Chytridiomycota, Mucoromycota, and Mortierellomycota, are advanced, including isolation procedure and media, incubation period and the slow growth of basidiomycetes, the identification of non-sporulating isolates, endophyte competition, and fungus–host interactions. We compare the detection of endophytes through culture-dependent methods and culture-independent methods, the role of fungi on senescence of the host plant, and next-generation studies. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Schizophyllan Optimization and Production in Submerged Cultures of Different Schizophyllum commune Isolates Collected in Thailand(2026-05-01) ;Chaimongkol, Soravit ;Yingkulchao, Wittayothin; ;Toan, Nguyen TaiWerapan, BorwornTwenty strains of Schizophyllum commune from the BIOTEC culture collection were selected for this study. S. commune is characterized by white to gray fan-shaped caps with lobed margins and distinctive split gills. Phylogenetic analysis of combined LSU rDNA and ITS rDNA sequences data using maximum parsimony placed the fungi in a strongly supported clade with S. commune. All strains were primarily screened for exopolysaccharide (EPS) and biomass production using potato dextrose broth (PDB) and peptone yeast glucose medium (PYGM) in 250 mL flasks shaken at 200 rpm for 7 days. The results revealed three strains with high EPS production, each exceeding 2.3 g/L, namely MMCR00487, MMCR00474 and MMCR00256. These strains were selected for media optimization using a Plackett–Burman design. Among them, MMCR00256 exhibited the highest EPS yield of 8.34 ± 1.47 g/L, followed by MMCR00487 and MMCR00474. Therefore, the strain MMCR00256 was further optimized by central composite design. The results revealed that the optimized medium for MMCR00256 increased the production of EPS to 10.39 ± 1.69 g/L, with a biomass yield of 26.28 ± 1.63 g/L (395 mg/g). The 5 L bioreactor optimization tested two inoculum types (mycelial and pellet) and two media (CCD and estimated) using strain MMCR00256. The mycelial inoculum grown in the estimated medium produced the highest EPS yield of 8.37 ± 0.26 g/L after 3 days, with 13.56 ± 2.94 g/L biomass. In conclusion, this study demonstrates that S. commune MMCR00256, when cultivated using the estimated medium and mycelial inoculum, can achieve enhanced exopolysaccharide production with improved efficiency, highlighting its significant potential for the development of efficient and scalable schizophyllan production processes at the industrial scale. Furthermore, this study provides essential insights into the cultivation and optimization of schizophyllan in S. commune.
