Sompornpailin, Kanokporn

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Sompornpailin, Kanokporn
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kanokporn.so@kmitl.ac.th

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    High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
    (2011-09-01)
    Chutipaijit, Sutee
    ;
    Cha-um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R <sup>2</sup> = 0.98) and anthocyanin (R <sup>2</sup> = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R <sup>2</sup> = 0.63) and anthocyanin (R <sup>2</sup> = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.
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    Differential accumulations of proline and flavonoids in indica rice varieties against salinity
    (2009-10-01)
    Chutipaijit, Sutee
    ;
    Cha-Um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Four varieties of indica rice differing in salt sensitivity were used for comparative study of defense systems in response to salinity. Rice seedlings were transferred into the photoautotrophic system containing NB medium with 100 mM NaCl as a salt stress treatment for 4 days. The physiological responses of jasmine rice (KDML105) and Sangyod (SY) varieties showed the better tolerance to salinity than those of Pathumthani 1 (PT1) and Black Sticky (BS) varieties. In salt stress treatment, the relative water content (RWC) of rice seedlings was decreased when compared to untreated seedlings. The RWC of salt-tolerant seedlings (KDML105 and SY) were decreased (0.33-0.53%) less than those of salt-sensitive seedlings (PT1 and BS), which exhibited 1.18-2.63% reductions. The salt-tolerant KDML105 and SY showed the lesser extent of membrane damage (lipid peroxidation; 7.94-19.26%) while enhanced the proline level (56.56-78.56%) and the flavonoid level (1.72-3.48%) more than those of the sensitive PT1 and BS varieties. Moreover, the positive correlation between proline or flavonoid accumulations and RWC was observed whereas the correlation between proline or flavonoid accumulations and MDA content was negative. These results suggest that high proline and flavonoids levels accumulated in each varieties of rice might be closely related to tolerant abilities indicating by physiological performances.
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    Increasing plant flavonoid biomaterials in response to UV-A light
    (2013-10-29)
    Kanthang, Supha
    ;
    Sompornpailin, Kanokporn
    Flavonoid biomaterials have a protecting function from various stresses. We examined the flavonoid biosynthesis in plant treated under visible light (VL) and additional UV-A light. The transgenic tobacco containing PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) cDNA, involved in flavonoid biosynthesis from Arabidopsis thaliana, were used for studying the flavonoid biosynthesis under both light conditions comparing to non transgenic tobacco. The flavonoid biomaterials were extracted with acidic methanol and water solvent from treated plant leaves. The absorbance of each biomaterial in the extract was measured under specific wavelength using a spectrophotometer. Additional UV-A radiated to non transgenic and transgenic tobacco affect the increasing of p-coumaric acid, naringenin, apigenin and kaempherol biomaterials from themselves grown under VL (approximately 120-130%). However, PAP1 transgenic tobaccos under additional UV-A radiation enhance the accumulation of these biomaterials up to160-180% higher than non transgenic tobaccos grown under VL condition. Moreover, PAP1 transgenic tobacco radiated with UVA light also significantly increased pelargonidin biomaterial. PAP1 transgenic tobaccos had a similar phenotype with non transgenic tobaccos but the color of fully expanding flower was more pink intensity than non transgenic. © (2013) Trans Tech Publications, Switzerland.
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    Property Comparison of Different Standard Wood Pallets for Possible Local Sourcing
    (2025-01-02)
    Manitayakul, Chutima
    ;
    Sompornpailin, Kanokporn
    The global expansion of freight transport across business types has impacted supply chain logistics in terms of coordinating the storage and delivery of goods and services across the supply chain. Wooden pallets are in high demand due to their price and environmental impact; however, the wood raw materials needed for making pallets are in short supply. This research was aimed at analyzing the possibility of using local wood instead of imported wood in the production of standard industrial pallets. Industrial pallets were designed using local acacia wood to replace some or all of the imported Radiata pine. This local acacia wood was utilized to prepare and construct pallets of the standard dimensions that were in accordance with the designs. Pallets made from the same type of wood and mixed woods were heat treated to meet the criteria of the ISPM 15 protocol for export. Flat pallet testing was conducted in accordance with industrial standards for the evaluation of the finished products. All types of pallets, including pallets containing local wood components, showed no visible damage during drop tests and compression strength assessments. However, the variability in diagonal span rates was influenced by the wood used in the fabrication process. Pallets frabricated from both partial and full acacia wood exhibited increased bending rates of 2081 and 2650 MPa, respectively, due to the higher levels of cellulose and lignin found in acacia wood. Our research suggests that there is an opportunity to manufacture standard pallets using locally sustainable wood resources, with some modifications to current industrial processes required.
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    Enhancement of plant regeneration efficiency from mature grains of thai indica rice (Oryza sativa L. cv. KDML105)
    (2012-08-01)
    Sompornpailin, Kanokporn
    ;
    Chutipaijit, Sutee
    In vitro protocol for efficient plant regeneration has been developed from Thai indica rice cultivar (Oryza sativa L. cv. KDML105). Embryogenic callus was initiated from mature seeds on NBMI medium. The highest frequency (96.00%) of callus induction was obtained on NBMI1 medium [2 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) without kinetin]. These calli were sub-cultured on NBM medium supplemented with various concentrations of BA or combination with supporting materials for plant regeneration. Plant regeneration from embryogenic callus was increased in the medium supplemented with 5 g L-1 Phytagel® in the regeneration medium (NBMR9 medium; 30.00%). In addition, the best regenerated frequency in this experiment was obtained by culturing the embryogenic callus to 4:4 ratios of Gro-lux tubes to fluorescent light tubes at 1000 lux light intensity (56.72%). The regenerated plants were successfully transplanted to soil. The results indicated that manipulation of medium supplements and the cultured condition leads to increase plant regeneration efficiency in KDML105 cultivar for genetic transformation in the future.
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    Proline accumulation and physiological responses of indica rice genotypes differing in tolerance to salt and drought stresses
    (2010-06-01)
    Chutipaijit, Sutee
    ;
    Suriyan, Cha Um
    ;
    Sompornpailin, Kanokporn
    The study determined the relationship between proline accumulation and physiological responses of indica rice genotypes to salt and drought stresses in photoautotrophic conditions. Two salt-tolerant genotypes of indica rice [cv. jasmine rice (KDML 105) and Dang], two drought-tolerant genotypes [Rice Department 6 (RD6) and Rice Department 15 (RD15)] and a salt-and drought-sensitive genotype [Pathumthani 1 (PT 1)] were exposed to 100 mM NaCl (salt stress) or 100 mM mannitol (drought stress) for 4 d. The relative water content (RWC) and malondialdehyde (MDA) accumulation in salt-and drought-tolerant genotypes did not significantly change. The proline accumulation in these genotypes greatly increased with stress. On the other hand, the salt-and drought-sensitive genotype which had lower proline accumulation demonstrated a significant decrease in RWC and increase in MDA content. Moreover, our results showed a strong correlation between proline concentrations and free radical scavenging by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The high concentration of proline solution presented high DPPH antioxidant activity. The higher proline accumulation in stressed seedlings of tolerant genotypes may help to maintain the RWC and MDA of rice plant, which could lead to preferable growth performance.
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    Novel Hybrid Spun Silk Yarn Developed from Eri and Mulberry Silks Under Industrial Level and Its Characterizations
    (2025-01-01)
    Kewcharoenwong, Preetanat
    ;
    Sompornpailin, Kanokporn
    To develop a novel hybrid spun silk yarn, the silk fibers have been analyzed in depth. This research aims to characterize the Mulberry and Eri silk fibers. The morphology of a single silk fiber was examined with an optical microscope and FESEM. The result of single-silk fiber properties showed that, Mulberry and Eri fibers have similar tensile strength (4.71 ± 0.98 and 4.93 ± 0.79 gram-force, respectively) but different in elongation percentage (13.43 ± 3.67 and 23.78 ± 4.90, respectively). Mulberry silk fibers are small, dense, compact, and non-porous, as for the Eri silk fibers, they are large, loose, and porous. Mulberry silk fibers have a smoother surface compared to Eri silk fibers. Mulberry and Eri silk fibers in different ratios were spun into silk yarn on an industrial scale, leading to the development of single and hybrid spun silk yarns. Under controlled yarn count and twist conditions, the hybrid silk yarns have an average tensile strength between that of single silk yarn. Adding more than 50% Mulberry silk fibers to spun silk yarn significantly reduced the percentage of yarn elongation. The synergistic effects of strength and elongation observed in Mulberry and Eri silk have resulted in novel yarns with new properties that can be applied in various textile industries.
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    An evaluation of water deficit tolerance screening in pigmented indica rice genotypes
    (2012-02-01)
    Chutipaijit, Sutee
    ;
    Cha-Um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Eight pigmented genotypes of indica subspecies were geminated and then treated by mannitol-induced water deficit stress. A change of growth characteristics, photosynthetic pigments, lipid peroxidation, DNA content, proline content and anthocyanin accumulation in stressed seedling (100 mM mannitol) and control plant (0 mM mannitol) were calculated. Growth performances, photosynthetic pigment concentrations, and DNA contents in all rice genotypes were dropped whereas proline, anthocyanin contents and the lipid peroxidation levels were enriched. The stabilization in total photosynthetic pigment concentrations of stressed-seedlings were positively correlated to the proline or anthocyanin accumulation. In contrast, MDA content, the increases in the percentages of drought-stressed seedlings were negatively correlated to the proline or anthocyanin accumulation. The changes in biochemical, physiological and growth parameters were subjected to Ward's cluster analysis for water deficit tolerance. These cultivars could be classified into two groups, water deficit sensitive, SY, KD, KLD and TD49 and water deficit tolerance, KS, KK1, KK2 and BSR.
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    Ozonation effectiveness on the mitigation of airborne microorganisms and particulate matter for enhanced indoor air quality
    (2026-01-01)
    Yooyen, Soemsak
    ;
    Pohsa, Akrom
    ;
    Hayeema, Asmin
    ;
    Fuengfung, Jakkarin
    ;
    Sookpordee, Pilun
    The efficacy of gaseous ozonation in eliminating indoor air pollutants has been investigated to improve indoor air quality. The ozone generator was operated until the air conditioning room attained ozone concentrations of 25, 50 and 75 ppm for 15 minutes. Rooms exposed to all ozone concentrations were efficacious in eradicating microorganisms and particulate matter (PM) within the air-conditioned room, with the level of elimination contingent upon the ozone concentration and duration of the test. Among these investigations, the 75 ppm ozone condition was the most efficacious in diminishing room microbe numbers. Ozone gas activity exerted a more pronounced effect on diminishing the number of PM. PMs within the 1.0–5.0 µm range were 75%–90% eliminated following the attainment of ozone levels of 75 ppm. This inquiry suggests that pre-treatment of the air with ozone can improve the indoor air quality and make it safer for occupants.
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    Effects of modulated concentration of ZnO nanoparticles on enhancing biosynthesis of metabolites and protecting plant membrane
    (2019-01-01)
    Chayaprasert, Wilailack
    ;
    Sompornpailin, Kanokporn
    Nowadays, nanoparticles are raising their applications around the world. ZnO nanoparticles are extensively used in the industrial and agricultural processes because of low price and less toxicity. Nevertheless, the pros and cons, effects of ZnO nanoparticles are still under discussing. This research aimed to study the protective effects of ZnO nanoparticles on plant physiology at the cellular level by analyzing biomaterial levels in relation to the stability of cellular membranes. Shoots of wild type and tt8 transgenic tobaccos were grown under tissue culture condition in the medium, adding 0, 10, 20 mg/L ZnO nanoparticles. Under media conditions adding 10, 20 mg/L ZnO nanoparticles, tobacco increased accumulation of plant metabolites (total soluble sugar and flavonoids). In these ZnO conditions, wild type and transgenic tobaccos enhanced total soluble sugar about 30-50% and 20-30%, respectively. The highest content of total soluble sugar was found in 20 mg/L ZnO nanoparticle condition. In similarity to the content of total soluble sugar, tobacco plants showed the highest average contents of flavone, flavonol and anthocyanin after growing in 20 mg/L ZnO nanoparticle conditions, 92.8%, 61.39% and 48.81% in wild type and 73.49%, 52.39% and 81.68% in transgenics, respectively. Tobaccos under 10 mg/L ZnO media condition increased the accumulation of total soluble sugar and flavonoid contents less than those under 20 mg/L ZnO media condition. However, these plants showed the lowest average percentage of cell membrane injury, 19.5% in wild type and 10-18% in transgenic tobaccos, following with plant under 20 mg/L ZnO nanoparticle and non ZnO nanoparticle conditions. Therefore, 10-20 mg/L ZnO nanoparticles in the media showed the induction of plant metabolites, especially in transgenics, and the enhancements of plant protection when compared with the same line tobacco under condition of non ZnO nanoparticles. Ten mg/L ZnO nanoparticle media is the best condition in protecting cellular membrane of tobacco.