Montri, Nattaya

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Montri, Nattaya
Alternative Name
Montri, N.
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Email
nattaya.mo@kmitl.ac.th

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    Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand
    (2020-12-08)
    Deewatthanawong, R.
    ;
    Tontiworachai, B.
    ;
    Kongchinda, P.
    ;
    Chanapan, S.
    ;
    Sriwilaiwan, S.
    Annona is a genus of tropical fruit trees in the family Annonaceae. Fruit pulp of Annona muricata contained the highest amount of gamma-aminobutyric acid (GABA) among Annona species. GABA content results from many factors including genetic makeup and environment. Influences of genetic variation and geographical environment on GABA content in fruit pulp of A. muricata were determined. To study genetic variation, sequences of ITS2 and psbA-trnH were used to construct phylogenetic trees. Young leaves of A. muricata were collected from different geographical locations including Chumphon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan Provinces in Thailand. Sequences of ITS2 and psbA-tmH were aligned and data sets were analyzed using maximum likelihood (ML) and neighbor-joining (NJ) methods. ITS2 sequence variations were observed within and among samples from different locations. A. muricata fruit harvested from eight provinces, namely Chumpon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan contained 19.75, 35.02, 32.91, 28.18, 42.37, 32.17, 65.02, and 31.58 mg 100 g-1 FW GABA, respectively. Findings indicated that both geographical environment and genetic variation had significant effects on GABA content in A. muricata fruit.
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    Production of F1 Papilionanthe hookeriana (Rchb.f.) Schltr. homozygous and heterozygous: Amplified fragment length polymorphism (AFLP) analysis of flower colors and self and cross-pollination ability between different flower colors
    (2023-11-01)
    Tontiworachai, B.
    ;
    Deewatthanawong, R.
    ;
    Intasam, N.
    ;
    Buamas, S.
    ;
    Promvai, S.
    Results showed that seed germination of Papilionanthe hookeriana (Rchb.f.) Schltr. was not significantly different between self-pollination treatments, while the percentage of selfpollination ability and seedling numbers were significantly different at 0.01 and 0.05 levels, respectively. Pink and purple flowers had the highest self-pollination ability at 100% and the highest numbers of seedlings (1,295) for self-pollination of pink flowers. Percentages of crosspollination ability, pod size and weight, and seed weight were not significantly different between treatments, while percentages of seed germination and seedling numbers were significantly different at the 0.01 level. The PixPu hybrid showed 100% seed germination, whereas the PixWh hybrid yielded the highest number of seedlings (403.2). Amplified fragment length polymorphism (AFLP) technique was used to study the genetic relations between various flower colors of P. hookeriana (Rchb.f.) Schltr. and the F1 hybrids of self- and cross-pollinated plants were classified into two clusters by UPGMA methods.
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    Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
    (2026-05-01)
    Tebdoie, C.
    ;
    Thipphaaut, T.
    ;
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Singhavorachai, P.
    The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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    Non-destructive measurement of Tetrahydrocannabinol (THC) and Cannabidiol (CBD) using near-infrared spectroscopy
    (2023-11-01)
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Chanapan, S.
    ;
    Tontiworachai, B.
    ;
    Sakkhamduang, C.
    Tetrahydrocannabinol (THC) and cannabidiol (CBD) are cannabinoids which produced by cannabis plants and major compounds found in cannabis products. A predictive method for non-destructive quantification of THC and CBD using near infrared spectroscopy (NIR) technology is developed. The prediction model for THC estimation had coefficient of determination (R-squared) and root mean square error of calibration (RMSEC) values of 0.9994 and 0.1926, respectively. The correlation between THC values of HPLC measurement and NIR prediction showed a correlation coefficient of 0.9078. For CBD prediction, the R-squared and RMSEC values of CBD equation were 0.9995 and 0.0006, respectively. The predicted and measured concentrations of CBD showed good correlation with a regression correlation of 0.9413. The test indicated NIR could be a promising alternative method for THC and CBD evaluation.
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    Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.
    (2024-09-01)
    Tontiworachai, B.
    ;
    Deewatthanawong, R.
    ;
    Mitsuda, N.
    ;
    Sakamoto, S.
    ;
    Chung, K.
    Cannabinoids are important secondary metabolites found in Cannabis sativa L. that play a crucial medical role in pain relief and mood adjustment. The main cannabinoid substances in cannabis are delta-9-tetrahydrocannabinolic acid (THCA), and cannabidiolic acid (CBDA). THCA biosynthesis is regulated by the THCA synthase (THCAS) gene which is regulated by the THCAS promoter. However, a comprehensive understanding regarding the regulation of gene expression by transcription factors (TFs) in cannabis is lacking. This study identified the TFs involved in regulating the THCAS promoter in Cannabis sativa L. ‘Hang Kra Rog’ by first amplifying the nucleotide sequences in the promoter of the THCAS gene from cannabis plants using the PCR technique. Subsequently, the THCAS promoter was cloned into the pENTR-5’-TOPO vector for sequencing purposes and then transferred into the R4L1pDEST_HISi vector by the LR reaction for yeast one-hybrid (Y1H) screening to study the interaction between TF proteins from the transcription factor cDNA library and the THCAS promoter within yeast cells. The TF proteins involved in the THCAS promoter were identified into seven groups: ARID, B3, bZIP, CPP, GeBP, HD, and NAC with further verification of their functions in cannabis plant cells using transient effector-reporter analysis.