Montri, Nattaya

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Montri, Nattaya
Alternative Name
Montri, N.
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Email
nattaya.mo@kmitl.ac.th

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    Item type:Publication,
    Preharvest Salicylic Acid Application Enhances Pigments, Antioxidants, and Secondary Metabolites in Cannabis sativa L.
    (2026-03-01)
    Montri, Nattaya
    ;
    Tebdoie, Chachpon
    ;
    Kongchinda, Papitchaya
    ;
    Singhavorachai, Pornjarus
    ;
    Tontiworachai, Borworn
    Salicylic acid (SA) is a key signaling molecule regulating secondary metabolism and stress responses in plants, but its preharvest role as a low-cost elicitor in cannabis remains underexplored. This study evaluated the effects of preharvest foliar SA application at different concentrations and application intervals on pigments, antioxidants, and cannabinoids in Cannabis sativa L. leaves and inflorescences. In leaves, moderate SA (0.1 M) significantly enhanced total phenolic content, total flavonoid content, and antioxidant activity (%DPPH inhibition), while higher concentrations suppressed these responses, reflecting a regulated metabolic trade-off rather than irreversible tissue damage. A significant interaction between SA concentration and preharvest time was observed for chlorophyll a (p < 0.01), whereas chlorophyll b and total chlorophyll were not significantly influenced by the interaction. In inflorescences, short-term application of 0.1 M SA (1 h preharvest) maximized phenolics, flavonoids, antioxidant capacity, and pigment accumulation, whereas the untreated controls showed the lowest levels. Cannabinoids exhibited distinct responses: Δ<sup>9</sup>-tetrahydrocannabinol (Δ<sup>9</sup>-THC), total tetrahydrocannabinol (Total THC), and tetrahydrocannabinolic acid (THCA) peaked at 0.1 M SA applied 1 h preharvest, while cannabidiol (CBD) was less concentration-dependent, with maximum accumulation observed at 1.0 M SA applied 24 h preharvest. Preharvest SA elicitation strongly modulated cannabis secondary metabolism. Short-term application of moderate SA promoted total phenolic, total flavonoid, antioxidant, pigment, and THC-group cannabinoid accumulation, while CBD displayed broader tolerance to concentration and application timing. These findings highlighted the potential of SA as a preharvest elicitor to improve cannabis phytochemical quality.
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    Effect of BA and IBA on micropropagation of Cannabis sativa L. ‘Hang Kra Rog’
    (2024-09-01)
    Singhavorachai, P.
    ;
    Deewatthanawong, R.
    ;
    Montri, N.
    ‘Hang Kra Rog’ is an original Thai strain of cannabis (Cannabis sativa L.). Tissue culture methods can rapidly propagate disease-free plants. This study optimized the protocol for surface sterilization and in vitro plant regeneration from explant nodal segments for the propagation of cannabis on an industrial scale. Various Clorox concentrations and soaking periods were used for surface sterilization, with different concentrations of BA and IBA plant growth regulators added to the basal medium for micropropagation of C. sativa L. using nodal explant culture. Results showed that 10% Clorox for 20 min followed by 5% Clorox for 20 min gave the highest explant survival at 75%. Optimal shoot and root development occurred when enriched with 0.5 mg L<sup>-1</sup> IBA, resulting in 1.00 shoots, 3.10 roots, 9.97 leaves, and a shoot length of 4.74 cm. The 2 mg L<sup>-1</sup> BA yielded the highest number of shoots at 1.87 shoots and resulted in a large callus size of 1.19 cm and callus fresh weight of 0.32 g.