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    Deep neural networks (DNNs) chemical compositions estimation of fresh durian in-line via near infrared spectroscopy
    (2025-06-01)
    Posom, Jetsada
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    Saenphon, Chirawan
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    Ditcharoen, Sirirak
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    Pitak, Lakkana
    ;
    Near infrared (NIR) spectroscopy and deep neural network (DNN) models were adopted for evaluating the nutritional compositions in durian pulp. The quality inspection of durians through online channels remains challenging because consumers cannot directly touch or smell the fruit. This leads to issues with substandard durians, such as unripe ones or those infested with pests. One hundred and sixty durian samples of Mon Thong varieties were used in this experiment. Then, the collected NIR spectra were augmented to improve the generalization ability of regression models. Deep neural network (DNNs) regressions were developed. The results showed that deep neural network (DNN) regression model possessed the best prediction performance, which were provided performance index. Almost all the best performance models were developed from the second derivative, except for the fat content model, which was developed from raw spectra. The best total soluble solids (TSS) model provided the coefficient of determination of calibration (R<sup>2</sup>c) and root mean square error of calibration (RMSEc) were 0.84 and 2.25 % and coefficient of determination of calibration (R<sup>2</sup>p), root mean square error of prediction (RMSEp) and ratio of performance to deviation (RPD) were 0.72 2.92 % and 1.92, respectively, and those of dry matter content (DMC) were provided R<sup>2</sup>c and RMSEc were 0.997, 0.58 %, and for prediction set provided R<sup>2</sup>p, RMSEp and RPD were 0.94, 3.13 %, and 4.21, respectively. For fat content (FC), they have also provided R<sup>2</sup>c and RMSEc were 0.84, 0.36 (g/100 g), while it provided R<sup>2</sup>p, RMSEp and RPD were 0.86, 0.50 (g/100 g), and 2.72, respectively. Moreover, for total sugar content (TSC) value, it also gave high accuracy, which provided R<sup>2</sup>c and RMSEc were 0.93, 0.49 (g/100 g) and R<sup>2</sup>p, RMSEp, and RPD were 0.91, 0.81 (g/100 g), 3.44, and for starch content (SC) also provided R<sup>2</sup>c and RMSEc were 0.93, 0.49(g/100 g) and R<sup>2</sup>p, RMSEp, and RPD were 0.76, 2.79 (g/100 g), 2.09, respectively. Therefore, the proposed method offers an ultrasensitive and effective strategy for estimation of nutritional compositions.
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    Modified specific gravity method for estimation of starch content and dry matter in cassava
    (2021-07-01)
    Maraphum, Kanvisit
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    Saengprachatanarug, Khwantri
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    Wongpichet, Seree
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    Phuphuphud, Arthit
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    An empirical model for the estimation of starch content (SC) and dry matter (DM) in cassava tubers was developed as an alternative method to polarimetry and dry oven. These improved estimation equations were developed based on the specific gravity (SG) method. To improve accuracy, the one hundred-seventy-four sample were obtained from four commercial varieties of cassava in Thailand including KU50, CMR38-125-77, RY9 and RY11, respectively. The age of sample collected from four to twelve months after planting was used in this experiment. The empirical model was created from their relationships between SG obtained from small sample size (~100 g) and its SC and DM. The SG for cassava was strongly correlated with the SC and DM, with values for the coefficient of determination (R<sup>2</sup>) of 0.81 and 0.83, respectively. The SC showed a high correlation with the DM, with R<sup>2</sup> of 0.96. To confirm that the empirical model was effective when applied to other samples, unknown samples collected from another area were tested, and the results showed a standard error of prediction (SEP) of 1.02%FW and 3.49%, mean different (MD) of -0.66%FW, -0.89% for the SC and DM, respectively. Hence, our empirical equation based on a modified SG method could be used to estimate the SC and DM in cassava tubers. It can help breeders to reduce costs and time requirements. Moreover, breeders could be used the methods to evaluate the SC and DM from the tuber formation to harvesting stage and monitoring the changes in SC and DM during breeding.
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    Evaluating limit of detection and quantification for higher heating value and ultimate analysis of fast-growing trees and agricultural residues biomass using NIRS
    (2023-10-09)
    Shrestha, Bijendra
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    Shrestha, Zenisha
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    Posom, Jetsada
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    Shrestha, Bim Prasad
    Accurate non-destructive assessment of biomass energy properties is essential for optimizing its use as an alternative fuel. In this study, 200 biomass samples were used to determine higher heating value (HHV) and 120 biomass samples for analyzing ultimate analysis parameters using near-infrared spectroscopy within the full wavenumber range of 12489.48 – 3594.87 cm<sup>-1</sup>. The samples were grounded, and five different types of partial least squares regression (PLSR) models were developed using traditional preprocessing, multi-preprocessing (MP) with 5 range, MP with 3 range, genetic algorithm, and successive projection algorithm. Limit of detection (LOD) and quantification (LOQ) were calculated using the best-performing model among five different PLSR models for HHV in kJ/kg, as well as the weight percentage (wt.%) of carbon (C), oxygen (O), hydrogen (H), and nitrogen (N). The LOD and LOQ for HHV were calculated as 622.42 kJ/kg and 1886.13 kJ/kg, respectively. Additionally, LOD and LOQ for ultimate analysis parameters, including C, O, H, and N were calculated as: 3.24 weight percentage (wt.%) and 9.81 wt.% for C, 2.04 wt.% and 6.18 wt.% for O, 0.35 wt.% and 1.05 wt.% for H, and 0.22 wt.% and 0.68 wt.% for N. The LOD and LOQ values for HHV, C, O, and H were lower than the minimum reference values used for model development, demonstrating the models’ high sensitivity and potential to reliably detect and precisely quantify these parameters. However, the LOD and LOQ values exceeded the minimum reference value used during model development for the N, indicating that the selected models have certain limitations in assessing the N content in biomass. The sample range should be expanded for wt.% of N to enhance the model’s performance, surpassing the LOD and LOQ values. This will improve the overall sensitivity of the model for reliable detection and quantification of N content in biomass samples.
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    Total soluble solids, dry matter content prediction and maturity stage classification of durian fruit using long-wavelength NIR reflectance
    (2023-12-01)
    Saenphon, Chirawan
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    Ditcharoen, Sirirak
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    Malai, Chayuttapong
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    Saengprachatanarug, Khwantri
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    Wongpichet, Seree
    The DM and TSS of durian pulp moving on a conveyor belt were measured for their rapid and non-destructive qualities based on quantitative and qualitative measurements. The calibration set and prediction set equaled 209 and 69 pulps, respectively. The quantitative test compared the performance of PLS regression for DM and TSS prediction developed from full wavelength (860–1754 nm) and a few significant variables using SPA, GA, and VIP methods. The qualitative test identified the possibility of maturity stage classification by comparing three supervised machine learning classifiers, namely SVM, random forest (RF), and LDA. Effective models for DM and TSS prediction were developed from second derivatives spectra combined with the GA method, exhibiting r<sup>2</sup>, SEP, and RPD of 0.85, 4.50%, and 2.64, respectively for DM, and 0.66, 5.15%, and 1.60, respectively, for TSS. The model classifying samples into two distinct groups, namely “reject” and “pass,” utilizing the LDA algorithm, exhibited an impressive accuracy rate of 94.20%, making it a suitable choice for quality assurance purposes. This result indicates that the few effective variables were more efficient than full wavelength and improved model accuracy with greater model stability. Enhancing the classification model could involve data sample balancing in each group, leading to further improvements.
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    NIR Spectroscopy for Non-Destructive Prediction of Greenhouse Gas Emissions and Global Warming Potential by Biomass Combustion
    (2026-05-01) ;
    Gyawali, Prakash
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    Posom, Jetsada
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    Shrestha, Bim Prasad
    Greenhouse gas (GHG) emissions from biomass combustion include carbon dioxide (CO<inf>2</inf>), methane (CH<inf>4</inf>) and nitrous oxide (N<inf>2</inf>O), which cause climate change and global warming. By measuring GHG emissions by biomass combustion, a potent protocol for the calculation of global warming potential (GWP), which is how much the global temperature has risen due to combustion processes, can be achieved, contributing to determining the mean reduction in global temperature rise and fostering a transition towards more sustainable energy systems. Additionally, warning can be given of the GHG and GWP risks associated with different species of biomass. This review includes the GHG emissions and GWP of biomass combustion and their measurement and estimation directly through biomass sample combustion, using unmanned aerial vehicles (UAVs) and satellite measurements of radiation interacting with atmospheric gases, or satellite-derived data and calculations according to IPCC guidelines. In addition, the relationship of lignocellulosic compounds and elements in biomass to HHV and GHG emissions is described. The key mechanism of molecular vibration of hydrogen bonds in biomass caused by NIR radiation related to GHG emissions is revealed and recorded regarding the possibility of using NIR spectroscopy for the prediction of GHG emissions and GWP. Calculation examples for sugarcane bagasse and other biomass species are shown. The comparative advantages and limitations of NIR spectroscopy with respect to other methods are included. These factors lead to elucidation of the possibility of using NIR spectroscopy for non-destructive prediction of GHG emissions. In this review, the feasibility of using NIR spectroscopy to evaluate GHG emissions, GWP and emission factors (EFs) as an alternative to IPCC estimation methods related to climate change by biomass combustion is confirmed. NIR spectroscopy is a novel methodology for predicting GHG emissions and GWP directly from intact chip or powder biomass spectral data without explicit gas measurement. This article records the essential spectroscopic knowledge of biomass polymer valorization that is of value in polymer science.
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    Integrating Vis-SWNIR spectrometer in a conveyor system for in-line measurement of dry matter content and soluble solids content of durian pulp
    (2021-11-01) ;
    Sharma, Sneha
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    Leepaitoon, Kritsanaphon
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    Chunsri, Rashphon
    The prediction of dry matter content (DMC) and soluble solids content (SSC) in durian pulp were performed using a small laboratory scale in-line visible and short wave near infrared (Vis-SWNIR) spectroscopic system. The fiber optic diode array spectrometer with a charged coupled device (CCD) detector in a wavelength range of 450−1000 nm was used for spectral data acquisition. The spectra of the sample were acquired on the moving conveyor belt in two different orientations, including scanning in the upright position of pulps collected in 2018 and the stable position by scanning on the side of the pulps collected in 2019. Partial least squares regression (PLSR) was used to establish the relationship between the spectra and observed DMC and SSC values using the different wavelength ranges, including 450−1000, 700−1000, and 800−1000 nm for the comparison. The results showed that the durian pulp should be scanned in the upright position at the center of the pulp. Moving average smoothing preprocessing combined with the standard normal variate (SNV) for DMC and multiple scatter correction (MSC) for SSC gave the best result. The suitable wavelength range for model development to predict the DMC and SSC was 700−1000 nm and 800−1000 nm, respectively. After comparing the results, the optimum model showed the coefficient of determination of calibration (R<inf>C</inf><sup>2</sup>), and prediction (R<inf>P</inf><sup>2</sup>), root mean square error of prediction (RMSEP), bias, and the ratio of performance to interquartile distance (RPIQ) of 0.88, 0.83, 4.32 %, 1.25 %, and 3.52 for DMC and 0.70, 0.70, 4.0 %, 0.4 %, and 2.2 for SSC prediction.
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    Item type:Publication,
    Effect of Combined Non-Wood and Wood Spectra of Biomass Chips on Rapid Prediction of Ultimate Analysis Parameters Using near Infrared Spectroscopy
    (2024-01-01)
    Shrestha, Bijendra
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    Posom, Jetsada
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    Shrestha, Bim Prasad
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    Funke, Axel
    The ultimate analysis parameters, including carbon (C), hydrogen (H), nitrogen (N), and oxygen (O) content in biomass, were rarely found to be predicted by non-destructive tests to date. In this research, we developed partial least squares regression (PLSR) models to predict the ultimate analysis parameters of chip biomass using near-infrared (NIR) raw spectra of non-wood and wood samples from fast-growing tree and agricultural residue and nine different traditional spectral preprocessing techniques. These techniques include first derivative (sd1), second derivative (sd2), constant offset, standard normal variate (SNV), multiplicative scatter correction (MSC), vector normalization, min-max normalization, mean centering, sd1 + vector normalization, and sd1 + MSC. Additionally, we employed a genetic algorithm (GA), successive projection algorithm (SPA), multi-preprocessing (MP) 5-range, and MP 3-range to develop a PLSR model for rapid prediction. A dataset consisting of 120 chip biomass samples was utilized for model development in which the samples were non-wood samples of 65–67% and wood samples of 33–35%, and the model performance was evaluated and compared. The selection of the optimum performing model was mainly based on criteria such as the coefficient of determination in the prediction set (R<sup>2</sup><inf>P</inf>), root mean square error of the prediction set (RMSEP), and the ratio of prediction to deviation (RPD). The optimal model for weight percentage (wt.%) of C was obtained using GA–PLSR, yielding R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.6954, 1.1252 wt.%, and 1.8, respectively. Similarly, for wt.% of O, the most effective model was obtained using the multi-preprocessing PLSR–5 range method with R<sup>2</sup><inf>P</inf> of 0.7150, RMSEP of 1.3088 wt.%, and RPD of 1.9. For wt.% of N, the optimal model was obtained using the MP PLSR-3 range method, resulting in R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.6073, 0.1008 wt.%, and 1.6, respectively. However, wt.% of the H model provided R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.5162, 0.2322 wt.%, and 1.5, respectively. Notably, the limit of quantification (LOQ) values for C, H, and O were lower than the minimum reference values used during model development, indicating a high level of sensitivity. However, the LOQ for N exceeded the minimum reference value, implying the samples to be predicted by the model must be in the range of reference range in the calibration set. By scatter plot analysis, the effect of combined non-wood and wood spectra of biomass chips on rapid prediction of ultimate analysis parameters using NIR spectroscopy was investigated. To include different species in a model, the species have to be not only in the different values of the constituents to make a wider range for a robust model, but also must provide their trend line characteristics in the scatter plot, i.e., correlation coefficient (R), slope, and intercept (same slope and slope approached to 1, and intercept is same (no gap) and approached zero, high R approached to 1). The effect of the R, slope, and intercept to obtain the better-optimized model was studied. The results show that the different species affected the model performance of each parameter prediction in a different manner, and by scatter plot analysis, which of these species were affecting the model negatively and how the model could be improved was indicated. This is the first time the effect has been studied by the principle of a scatter plot.
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    Two different portables visible-near infrared and shortwave infrared region for on-tree measurement of soluble solid content of marian plum fruit
    (2020-01-01)
    Posom, Jetsada
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    Soonnamtiang, Navavit
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    Kotethum, Patcharapong
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    Konjun, Pakhpoom
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    The goal of this study was to predict the soluble solid content (SSC) of on-tree Marian plum fruit using two different wavelength range and algorithm. One of these was the commercial dispersion NIR spectrometer (MicroNIR 1700), providing shortwave infrared (SWIR), while the other was a making diode array spectrometer giving visible-near infrared (Vis-NIR). To search optimal model, the analytical ability of the two wavelength ranges spectrometers coupled with two algorithms: i.e. partial least squares regression (PLSR) and support vector machine regression (SVR), were investigated. Different spectral pre-processing methods were tested. The model providing the lowest root mean square errors of prediction (RMSEP) was selected. Overall, the proposed outcome was that the performance of SWIR was more accurate than Vis-NIR spectrometer, and that both SWIR and Vis-NIR coupled with PLSR algorithm had a higher accuracy than SVR algorithm. The best model for on-tree evaluation SSC was the SWIR constructed using the PLSR algorithm with the spectral pre-processing of the 2<sup>nd</sup> derivative, providing a coefficient of determination of calibration set (R<sup>2</sup>) of 0.81, a coefficient of determination of validation set (r<sup>2</sup>) of 0.76, RMSEP of 0.69 °Brix, and a relative standard error of prediction (RSEP) of 4.43%. The outcome showed that a portable SWIR spectrometer developed with PLSR could be used for monitoring the SSC of individual Marian plum fruit on-tree for quality assurance.
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    Near-infrared spectroscopy, hyperspectral, multispectral imaging principles and applications in energy properties of biomass
    (2023-08-21)
    Posom, Jetsada
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    Shrestra, Bijendra
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    Maraphum, Kanvisit
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    Pitak, Lakkana
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    Saengprachatanarug, Khwantri
    Biomass is renewable energy which is zero neutrality carbon energy. It is used for generating heat energy and electrical energy. Therefore, the use of biomass with high efficiency is important and the quality of biomass related to its energy should be measured before utilization and trading. The measurement of energy indexes of biomass is necessary to the thermal conversion process and the trading of biomass. However, the conventional measurement methods are laborious and take a long time, with a lot of costs. In recent years, near infrared spectroscopy (NIR) and imaging technologies (hyperspectral and multispectral images) have been widely investigated and applied as non-destructive, reliable and accurate techniques to monitor the quality and composition of biomass. This chapter contains the principle of NIR and imaging technique including essential component principles, NIR and imaging technique procedures, novel model development methods and applications. The non-destructive measurement of biomass quality as the real time and non- contact measurement will be represented. Moreover, this chapter will describe the application of NIR and imaging techniques for analysing the energy indexes of biomass, such as heating value or calorific value, proximate data, elemental composition, combustion index, pyrolysis characteristics, mechanical properties and so on.
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    Predicting biomass global warming potential with FT-NIR spectroscopy
    (2025-12-01)
    Gyawali, Prakash
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    Shrestha, Bijendra
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    Posom, Jetsada
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    This research is to predict the global warming potential (GWP) of biomass by using Fourier transform near-infrared (FT-NIR) spectroscopy. A partial least squares regression model of 197 biomass chip samples was developed for predicting GWP of fast-growing trees and agricultural residues. The reference value of GWP of biomass sample was calculated by the method provided by Intergovernmental Panel on Climate Change (IPCC). After applying different spectral pretreatments and variable selection methods, the best model for predicting GWP was found using the 1st derivative spectrum pretreatment and covariance method (COVM) based variable selection. The results indicate GWP model exhibit good predictive capabilities, where the model can be usable with caution for any purpose including research, by achieving a coefficient of determination for prediction set (R<sup>2</sup><inf>P</inf>) of 0.86, and ratio of prediction to deviation (RPD) of 2.6. Additionally, the RMSEP of 0.00063 suggests a low prediction error. This pioneering approach presents a swift and efficient means to determine GWP, the complex functionality parameter, which reveals an optimal relationship model, showcasing its efficacy in a significant advancement in the assessment of biomass functionality related to climate change issue. Additionally, the further research is recommended to integrate FT-NIR data with thermogravimetric analyser to simulate of different thermal conversion of biomass type where different emission gases are generated and with gas chromatography–mass spectrometry for evaluation of concentration of the generated gases for further refine GWP predictions which providing more comprehensive insights and exact content of emission gases affect global warming to support the IPCC.