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    Deep neural networks (DNNs) chemical compositions estimation of fresh durian in-line via near infrared spectroscopy
    (2025-06-01)
    Posom, Jetsada
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    Saenphon, Chirawan
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    Ditcharoen, Sirirak
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    Pitak, Lakkana
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    Near infrared (NIR) spectroscopy and deep neural network (DNN) models were adopted for evaluating the nutritional compositions in durian pulp. The quality inspection of durians through online channels remains challenging because consumers cannot directly touch or smell the fruit. This leads to issues with substandard durians, such as unripe ones or those infested with pests. One hundred and sixty durian samples of Mon Thong varieties were used in this experiment. Then, the collected NIR spectra were augmented to improve the generalization ability of regression models. Deep neural network (DNNs) regressions were developed. The results showed that deep neural network (DNN) regression model possessed the best prediction performance, which were provided performance index. Almost all the best performance models were developed from the second derivative, except for the fat content model, which was developed from raw spectra. The best total soluble solids (TSS) model provided the coefficient of determination of calibration (R<sup>2</sup>c) and root mean square error of calibration (RMSEc) were 0.84 and 2.25 % and coefficient of determination of calibration (R<sup>2</sup>p), root mean square error of prediction (RMSEp) and ratio of performance to deviation (RPD) were 0.72 2.92 % and 1.92, respectively, and those of dry matter content (DMC) were provided R<sup>2</sup>c and RMSEc were 0.997, 0.58 %, and for prediction set provided R<sup>2</sup>p, RMSEp and RPD were 0.94, 3.13 %, and 4.21, respectively. For fat content (FC), they have also provided R<sup>2</sup>c and RMSEc were 0.84, 0.36 (g/100 g), while it provided R<sup>2</sup>p, RMSEp and RPD were 0.86, 0.50 (g/100 g), and 2.72, respectively. Moreover, for total sugar content (TSC) value, it also gave high accuracy, which provided R<sup>2</sup>c and RMSEc were 0.93, 0.49 (g/100 g) and R<sup>2</sup>p, RMSEp, and RPD were 0.91, 0.81 (g/100 g), 3.44, and for starch content (SC) also provided R<sup>2</sup>c and RMSEc were 0.93, 0.49(g/100 g) and R<sup>2</sup>p, RMSEp, and RPD were 0.76, 2.79 (g/100 g), 2.09, respectively. Therefore, the proposed method offers an ultrasensitive and effective strategy for estimation of nutritional compositions.
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    Evaluating limit of detection and quantification for higher heating value and ultimate analysis of fast-growing trees and agricultural residues biomass using NIRS
    (2023-10-09)
    Shrestha, Bijendra
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    Shrestha, Zenisha
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    Posom, Jetsada
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    Shrestha, Bim Prasad
    Accurate non-destructive assessment of biomass energy properties is essential for optimizing its use as an alternative fuel. In this study, 200 biomass samples were used to determine higher heating value (HHV) and 120 biomass samples for analyzing ultimate analysis parameters using near-infrared spectroscopy within the full wavenumber range of 12489.48 – 3594.87 cm<sup>-1</sup>. The samples were grounded, and five different types of partial least squares regression (PLSR) models were developed using traditional preprocessing, multi-preprocessing (MP) with 5 range, MP with 3 range, genetic algorithm, and successive projection algorithm. Limit of detection (LOD) and quantification (LOQ) were calculated using the best-performing model among five different PLSR models for HHV in kJ/kg, as well as the weight percentage (wt.%) of carbon (C), oxygen (O), hydrogen (H), and nitrogen (N). The LOD and LOQ for HHV were calculated as 622.42 kJ/kg and 1886.13 kJ/kg, respectively. Additionally, LOD and LOQ for ultimate analysis parameters, including C, O, H, and N were calculated as: 3.24 weight percentage (wt.%) and 9.81 wt.% for C, 2.04 wt.% and 6.18 wt.% for O, 0.35 wt.% and 1.05 wt.% for H, and 0.22 wt.% and 0.68 wt.% for N. The LOD and LOQ values for HHV, C, O, and H were lower than the minimum reference values used for model development, demonstrating the models’ high sensitivity and potential to reliably detect and precisely quantify these parameters. However, the LOD and LOQ values exceeded the minimum reference value used during model development for the N, indicating that the selected models have certain limitations in assessing the N content in biomass. The sample range should be expanded for wt.% of N to enhance the model’s performance, surpassing the LOD and LOQ values. This will improve the overall sensitivity of the model for reliable detection and quantification of N content in biomass samples.
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    Application of baby corn husk as a biological sustainable feedstock for the production of cellulase and xylanase by Lentinus squarrosulus Mont.
    (2023-02-01)
    Vichitraka, Asanee
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    Tantratian, Sumate
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    In an effort to use baby corn husk (BCH) as a sustainable feedstock for cellulase and xylanase production by the Lentinus squarrosulus Mont. isolate LS-YA (LSM-LS-YA), a suitable pretreatment method and fermentation strategies were developed. BCH pretreated with 1 M sodium hydroxide for 90 min, an alkaline pretreatment, exemplified an appropriate pretreatment method. In a 10-L external Venturi injector bioreactor, the highest cellulase and xylanase production was 4.12 ± 0.36 unit/mL and 6.15 ± 0.36 unit/mL, respectively, when 1 g/L diammonium hydrogen phosphate was used as the nitrogen source and the aeration rate was controlled at 0.2 vvm. This study provides an informative perspective on the production of cellulase and xylanase from agricultural lignocellulosic materials, which could reduce agricultural waste while supporting a zero-waste circular economy, and this fermentation process would be applicable to larger-scale production.
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    Analysis of the Pomelo Peel Essential Oils at Different Storage Durations Using a Visible and Near-Infrared Spectroscopic on Intact Fruit
    (2024-08-01) ;
    Duangchang, Jittra
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    Shrestha, Bim Prasad
    Pomelo fruit pulp mainly is consumed fresh and with very little processing, and its peels are discarded as biological waste, which can cause the environmental problems. The peels contain several bioactive chemical compounds, especially essential oils (EOs). The content of a specific EO is important for the extraction process in industry and in research units such as breeding research. The explanation of the biosynthesis pathway for EO generation and change was included. The chemical bond vibration affected the prediction of EO constituents was comprehensively explained by regression coefficient plots and x-loading plots. Visible and near-infrared spectroscopy (VIS/NIRS) is a prominent rapid technique used for fruit quality assessment. This research work was focused on evaluating the use of VIS/NIRS to predict the composition of EOs found in the peel of the pomelo fruit (Citrus maxima (J. Burm.) Merr. cv Kao Nam Pueng) following storage. The composition of the peel oil was analyzed by gas chromatography–mass spectrometry (GC-MS) at storage durations of 0, 15, 30, 45, 60, 75, 90, 105 and 120 days (at 10 °C and 70% relative humidity). The relationship between the NIR spectral data and the major EO components found in the peel, including nootkatone, geranial, β-phellandrene and limonene, were established using the raw spectral data in conjunction with partial least squares (PLS) regression. Preprocessing of the raw spectra was performed using multiplicative scatter correction (MSC) or second derivative preprocessing. The PLS model of nootkatone with full MSC had the highest correlation coefficient between the predicted and reference values (r = 0.82), with a standard error of prediction (SEP) of 0.11% and bias of 0.01%, while the models of geranial, β-phellandrene and limonene provided too low r values of 0.75, 0.75 and 0.67, respectively. The nootkatone model is only appropriate for use in screening and some other approximate calibrations, though this is the first report of the use of NIR spectroscopy on intact fruit measurement for its peel EO constituents during cold storage.
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    Revealing the Power of Deep Learning in Quality Assessment of Mango and Mangosteen Purée Using NIR Spectral Data
    The quality control of fruit purée products such as mango and mangosteen is crucial for maintaining consumer satisfaction and meeting industry standards. Traditional destructive techniques for assessing key quality parameters like the soluble solid content (SSC) and titratable acidity (TA) are labor-intensive and time-consuming; prompting the need for rapid, nondestructive alternatives. This study investigated the use of deep learning (DL) models including Simple-CNN, AlexNet, EfficientNetB0, MobileNetV2, and ResNeXt for predicting SSC and TA in mango and mangosteen purée and compared their performance with the conventional chemometric method partial least squares regression (PLSR). Spectral data were preprocessed and evaluated using 10-fold cross-validation. For mango purée, the Simple-CNN model achieved the highest predictive accuracy for both SSC (coefficient of determination of cross-validation ((Formula presented.)) = 0.914, root mean square error of cross-validation (RMSE<inf>CV</inf>) = 0.688, the ratio of prediction to deviation of cross-validation (RPD<inf>CV</inf>) = 3.367) and TA ((Formula presented.) = 0.762, RMSE<inf>CV</inf> = 0.037, RPD<inf>CV</inf> = 2.864), demonstrating a statistically significant improvement over PLSR. For the mangosteen purée, AlexNet exhibited the best SSC prediction performance ((Formula presented.) = 0.702, RMSE<inf>CV</inf> = 0.471, RPD<inf>CV</inf> = 1.666), though the RPD<inf>CV</inf> values (<2.0) indicated limited applicability for precise quantification. TA prediction in mangosteen purée showed low variance in the reference values (standard deviation (SD) = 0.048), which may have restricted model performance. These results highlight the potential of DL for improving NIR-based quality evaluation of fruit purée, while also pointing to the need for further refinement to ensure interpretability, robustness, and practical deployment in industrial quality control.
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    Total soluble solids, dry matter content prediction and maturity stage classification of durian fruit using long-wavelength NIR reflectance
    (2023-12-01)
    Saenphon, Chirawan
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    Ditcharoen, Sirirak
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    Malai, Chayuttapong
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    Saengprachatanarug, Khwantri
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    Wongpichet, Seree
    The DM and TSS of durian pulp moving on a conveyor belt were measured for their rapid and non-destructive qualities based on quantitative and qualitative measurements. The calibration set and prediction set equaled 209 and 69 pulps, respectively. The quantitative test compared the performance of PLS regression for DM and TSS prediction developed from full wavelength (860–1754 nm) and a few significant variables using SPA, GA, and VIP methods. The qualitative test identified the possibility of maturity stage classification by comparing three supervised machine learning classifiers, namely SVM, random forest (RF), and LDA. Effective models for DM and TSS prediction were developed from second derivatives spectra combined with the GA method, exhibiting r<sup>2</sup>, SEP, and RPD of 0.85, 4.50%, and 2.64, respectively for DM, and 0.66, 5.15%, and 1.60, respectively, for TSS. The model classifying samples into two distinct groups, namely “reject” and “pass,” utilizing the LDA algorithm, exhibited an impressive accuracy rate of 94.20%, making it a suitable choice for quality assurance purposes. This result indicates that the few effective variables were more efficient than full wavelength and improved model accuracy with greater model stability. Enhancing the classification model could involve data sample balancing in each group, leading to further improvements.
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    NIR Spectroscopy for Non-Destructive Prediction of Greenhouse Gas Emissions and Global Warming Potential by Biomass Combustion
    (2026-05-01) ;
    Gyawali, Prakash
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    Posom, Jetsada
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    Shrestha, Bim Prasad
    Greenhouse gas (GHG) emissions from biomass combustion include carbon dioxide (CO<inf>2</inf>), methane (CH<inf>4</inf>) and nitrous oxide (N<inf>2</inf>O), which cause climate change and global warming. By measuring GHG emissions by biomass combustion, a potent protocol for the calculation of global warming potential (GWP), which is how much the global temperature has risen due to combustion processes, can be achieved, contributing to determining the mean reduction in global temperature rise and fostering a transition towards more sustainable energy systems. Additionally, warning can be given of the GHG and GWP risks associated with different species of biomass. This review includes the GHG emissions and GWP of biomass combustion and their measurement and estimation directly through biomass sample combustion, using unmanned aerial vehicles (UAVs) and satellite measurements of radiation interacting with atmospheric gases, or satellite-derived data and calculations according to IPCC guidelines. In addition, the relationship of lignocellulosic compounds and elements in biomass to HHV and GHG emissions is described. The key mechanism of molecular vibration of hydrogen bonds in biomass caused by NIR radiation related to GHG emissions is revealed and recorded regarding the possibility of using NIR spectroscopy for the prediction of GHG emissions and GWP. Calculation examples for sugarcane bagasse and other biomass species are shown. The comparative advantages and limitations of NIR spectroscopy with respect to other methods are included. These factors lead to elucidation of the possibility of using NIR spectroscopy for non-destructive prediction of GHG emissions. In this review, the feasibility of using NIR spectroscopy to evaluate GHG emissions, GWP and emission factors (EFs) as an alternative to IPCC estimation methods related to climate change by biomass combustion is confirmed. NIR spectroscopy is a novel methodology for predicting GHG emissions and GWP directly from intact chip or powder biomass spectral data without explicit gas measurement. This article records the essential spectroscopic knowledge of biomass polymer valorization that is of value in polymer science.
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    Huanglongbing (HLB) disease detection using drone imagery and deep learning neural networks for early management of HLB
    (2026-08-01)
    Kalbande, Vishal Dashrathrao
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    Sripinyowanich Jongyingcharoen, Jiraporn
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    In the modern world, machine learning and artificial intelligence have become the foundation of the digital revolution and have a significant role in daily life. It is adopted in various applications, such as object detection, recognition and classification. This research aimed to use deep learning algorithms for the detection of Huanglongbing (HLB)-infected disease, healthy and background patch in citrus orchard. HLB-infected patches need to be identified for appropriate treatments of spraying. However, farmers are unaware of infections on plant leaves and therefore adopt manual disease identification methods. This method results in loss of productivity as the infection spreads throughout the field. However, due to a lack of required facilities, instant identification needs to be improved in many aspects of the agricultural sector. To conduct this research, firstly, a dataset was created that contained drone images of citrus orchards that was categorized into three classes, specifically (i) Healthy, (ii) HLB-infected and (iii) Background. Under this research, 6000 image patches of citrus orchard were collected and categorized 2000 images in each class based on appropriate labels. The next step was to train the deep learning models to identify Healthy, HLB-infected and Background. In this research three models viz. EfficientNetV2B0, DenseNet-121 and ResNet-50 were trained to detect the HLB infection patch in the citrus orchard. The models exhibited exceptional performance, with ResNet-50 achieving the highest overall accuracy of 89%, followed by EfficientNet-V2B0 (87%) and DenseNet-121 (85%). ResNet-50 demonstrated superior balanced performance with macro-average precision of 90%, recall of 89%, and F1-score of 89%. The generated HLB detection map effectively visualized disease distribution patterns, identifying 31% of patches as HLB-infected, 7% as Healthy and 62% as Background across the orchard grid. This research establishes a reliable framework for large-scale, automated citrus disease monitoring that enables precision agriculture interventions, potentially reducing economic losses and optimizing resource allocation in citrus cultivation through early and accurate HLB disease detection.
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    Effect of Combined Non-Wood and Wood Spectra of Biomass Chips on Rapid Prediction of Ultimate Analysis Parameters Using near Infrared Spectroscopy
    (2024-01-01)
    Shrestha, Bijendra
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    Posom, Jetsada
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    Shrestha, Bim Prasad
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    Funke, Axel
    The ultimate analysis parameters, including carbon (C), hydrogen (H), nitrogen (N), and oxygen (O) content in biomass, were rarely found to be predicted by non-destructive tests to date. In this research, we developed partial least squares regression (PLSR) models to predict the ultimate analysis parameters of chip biomass using near-infrared (NIR) raw spectra of non-wood and wood samples from fast-growing tree and agricultural residue and nine different traditional spectral preprocessing techniques. These techniques include first derivative (sd1), second derivative (sd2), constant offset, standard normal variate (SNV), multiplicative scatter correction (MSC), vector normalization, min-max normalization, mean centering, sd1 + vector normalization, and sd1 + MSC. Additionally, we employed a genetic algorithm (GA), successive projection algorithm (SPA), multi-preprocessing (MP) 5-range, and MP 3-range to develop a PLSR model for rapid prediction. A dataset consisting of 120 chip biomass samples was utilized for model development in which the samples were non-wood samples of 65–67% and wood samples of 33–35%, and the model performance was evaluated and compared. The selection of the optimum performing model was mainly based on criteria such as the coefficient of determination in the prediction set (R<sup>2</sup><inf>P</inf>), root mean square error of the prediction set (RMSEP), and the ratio of prediction to deviation (RPD). The optimal model for weight percentage (wt.%) of C was obtained using GA–PLSR, yielding R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.6954, 1.1252 wt.%, and 1.8, respectively. Similarly, for wt.% of O, the most effective model was obtained using the multi-preprocessing PLSR–5 range method with R<sup>2</sup><inf>P</inf> of 0.7150, RMSEP of 1.3088 wt.%, and RPD of 1.9. For wt.% of N, the optimal model was obtained using the MP PLSR-3 range method, resulting in R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.6073, 0.1008 wt.%, and 1.6, respectively. However, wt.% of the H model provided R<sup>2</sup><inf>P</inf>, RMSEP, and RPD values of 0.5162, 0.2322 wt.%, and 1.5, respectively. Notably, the limit of quantification (LOQ) values for C, H, and O were lower than the minimum reference values used during model development, indicating a high level of sensitivity. However, the LOQ for N exceeded the minimum reference value, implying the samples to be predicted by the model must be in the range of reference range in the calibration set. By scatter plot analysis, the effect of combined non-wood and wood spectra of biomass chips on rapid prediction of ultimate analysis parameters using NIR spectroscopy was investigated. To include different species in a model, the species have to be not only in the different values of the constituents to make a wider range for a robust model, but also must provide their trend line characteristics in the scatter plot, i.e., correlation coefficient (R), slope, and intercept (same slope and slope approached to 1, and intercept is same (no gap) and approached zero, high R approached to 1). The effect of the R, slope, and intercept to obtain the better-optimized model was studied. The results show that the different species affected the model performance of each parameter prediction in a different manner, and by scatter plot analysis, which of these species were affecting the model negatively and how the model could be improved was indicated. This is the first time the effect has been studied by the principle of a scatter plot.
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    Moisture content prediction in durian husk biomass via near infrared spectroscopy coupled with aquaphotomics and explainable machine learning
    (2025-12-15)
    Shrestha, Zenisha
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    Shrestha, Bijendra
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    Pun, Umed Kumar
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    Bajracharya, Tri Ratna
    Accurate determination of moisture content is essential for energy efficiency and biomass management for fuel materials such as durian husk. Traditional methods of determining biomass moisture content are time-consuming and require specialized expertise, posing challenges for continuous monitoring. To address this limitation, this study applies Near Infrared Spectroscopy (NIRS) combined with machine learning models to rapidly and accurately assess moisture content. Both linear Partial Least Squares Regression (PLSR) and non-linear approaches were used, including Support Vector Machines (SVM), Artificial Neural Networks (ANN), and Extreme Gradient Boosting (XGB). The application of preprocessing techniques, notably the Savitzky-Golay second derivative (SD) and Standard Normal Variate (SNV), significantly augmented the predictive performance, highlighting the importance of data preprocessing in spectral analysis. Synthetic spectral augmentation using Gaussian noise revealed that while SVM and ANN exhibited near-perfect performance, SVM demonstrated quantifiable reliability. This study also demonstrates SVM as the most sensitive and reliable method for detecting and quantifying moisture content in durian husk. This research contributes novel insights to biomass analysis, highlighting the benefits of integrating NIRS and feasibility of explainable machine learning techniques to identify water related spectral parameters to advance aquaphotomics, thereby advancing rapid and accurate biomass characterization.