Saetiew, Kanjana

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Saetiew, Kanjana
Alternative Name
Saetiew, K.
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Email
kanjana.sa@kmitl.ac.th

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    Effect of ethyl methanesulfonate on growth and morphology of Doritis pulcherrima Lindl.
    (2026-01-01)
    Supreeworakij, S.
    ;
    Saetiew, K.
    ;
    Montri, N.
    Results showed that 0.5 mg/l BA and 1 mg/l NAA promoted the greatest seedling growth, with increased leaf number, leaf length, plant height, and root development. In the third experiment, protocorm-like bodies were treated with EMS at 0, 0.5, and 1.0% for 60 or 90 minutes. Survival declined with increasing concentration and time, with LD₅₀ values of 0.82% at 60 minutes and 0.54% at 90 minutes. Chlorophyll a, Chlorophyll b, and carotenoids were not significantly affected, but stomatal size changed. Stomatal width and length increased under 1.0% EMS, while density decreased from 220.17 to 149.47 stomata/mm². EMS treatment for 90 minutes showed an increased density relative to 60 min. RAPD analysis using 10 primers revealed high polymorphism indicating EMS induced genetic variation. Cluster analysis divided 22 samples into 9 groups at a similarity coefficient of 0.89, confirming genetic diversity despite no visible morphological changes.
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    Callus and hairy root induction of Melaleuca cajuputi Powell
    (2020-05-01)
    Prangthong, U.
    ;
    Montri, N.
    ;
    Saetiew, K.
    It is stated that the lowest percentage of contamination at 49.63% and highest percentage of survival at 37.95% after sterilized with 70% ethanol for 1 min and 1.8 % NaOCl for 30 min. The bud explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.02, 0.04, 0.06, 0.08 mg/l 2,4-Dinitrophenylhylhydrazine (2,4-D) combination with 0, 0.02, 0.04, 0.06, and 0.08 mg/l Thidiazuron (TDZ) for callus induction. The results showed that the explants were cultured on MS medium supplemented with 0.06 mg/l TDZ resulting to be suitable for callus induction with biggest size at 65.2x1. 22cm of width and length. Culturing buds on MS medium supplemented with 0.02 mg/l TDZ had the highest averaged shoot number. The roots were cultured in liquid MS medium supplemented with 0.04 mg/l TDZ, 0.04 mg/l 2.4-D gave the highest weight of hairy roots.
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    Effects of organic compound and plant growth regulators on seed germination and growth of Doritis pulcherrima Lindl. in vitro
    (2024-09-01)
    Supreeworakij, S.
    ;
    Saetiew, K.
    ;
    Rungkitwattananukul, T.
    ;
    Phanchompoo, P.
    ;
    Montri, N.
    Doritis pulcherrima Lindl. is one of the wild orchids of which the distribution has decreased in nature and endangered. In vitro seed culture by using plant tissue culture is a proper method to help increasing the numbers. The purpose of this research was to study the effect of organic compound and plant growth regulators on seed germination and growth of D. pulcherrima in vitro. Seeds were cultured on Vacin and Went (VW) basal medium. Experiments were performed in a 5×7 factorial in completely randomized design (factorial in CRD) 2 factors; Factor 1 capsule of D. pulcherrima with 5 treatments such as Pi102/1, Pi102/2, Pi119/1, Pi119/2 and Pu112. Factor 2 organic compound with 7 treatments such as no organic compound (control), 50, 100 g L<sup>-1</sup> raw namwa banana powder, 50, 100 g L<sup>-1</sup> cavendish banana homogenate, 50, 100 g L<sup>-1</sup> potato homogenate. After 13 weeks of culture, there were only 3 capsules growth seed germination (Pi119/2, Pi102/2 and Pu112/1) and the highest percentage of seed germination was Pi119/2 on VW medium supplemented with 50, 100 g L<sup>-1</sup> banana powder and 50 g L<sup>-1</sup> potato homogenate gave the highest germination percentage at level 4 (75-100%). From the results, organic compound is a suitable culture supplement for D. pulcherrima seed germination and development compared to no organic compound. In addition, the effect of plant growth regulators on growth and development of D. pulcherrima protocorm were cultured on VW medium supplemented with different concentrations of control, 1, 2 and 3 mg L<sup>-1</sup> BA and 0.01, 0.02 and 0.03 mg L<sup>-1</sup>TDZ. The result showed the control gave the highest number of leaves at 6.50, the number of protocorm at 3.67 and fresh weight of protocorm at 0.42 g. The protocorm and plantlet were induced after 10 of culture.